Data/hora: 02/06/2024 07:07:03

Provedor de dados: | 63
País: | Brazil
Título: | A simple method for DNA isolation from Xanthomonas spp.
Autores: | Gomes,Luiz Humberto; Duarte,Keila Maria Roncato; Andrino,Felipe Gabriel; Tavares,Flavio Cesar Almeida.
Data: | 2000-09-01
Ano: | 2000
Palavras-chave: | DNA; Xanthomonas; RAPD.
Resumo: | A simple DNA isolation method was developed with routine chemicals that yields high quality and integrity preparations when compared to some of the most well known protocols. The method described does not require the use of lysing enzymes, water bath and the DNA was obtained within 40 minutes The amount of nucleic acid extracted (measured in terms of absorbancy at 260 nm) from strains of Xanthomonas spp., Pseudomonas spp. and Erwinia spp. was two to five times higher than that of the most commonly used method.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162000000300028
Editor: | São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação: | 10.1590/S0103-90162000000300028
Formato: | text/html
Fonte: | Scientia Agricola  v.57 n.3 2000
Fonte: | info:eu-repo/semantics/openAccess