Data/hora: 06/06/2024 03:41:21

Provedor de dados: | 65
País: | Brazil
Título: | Surubim-do-Paraíba oocytes viability after being exposed to different cryoprotectants
Autores: | Lopes,Taís da Silva; Sanches,Eduardo Antônio; Caneppele,Danilo; Romagosa,Elizabeth.
Data: | 2018-01-01
Ano: | 2018
Palavras-chave: | Fish reproduction; Fish oocytes; Toxicity; Concentrations of cryoprotecntants; Steindachneridion parahybae.
Resumo: | ABSTRACT: To know the non-toxic cryoprotectants to fish oocytes is of extreme importance for tests that aim to increase oocyte resistance to cold, thus allowing more advanced studies in cryopreservation. Therefore, commonly used cryoprotectants such as methanol, dimethyl sulfoxide, ethylene glycol, propylene glycol, sucrose and fructose were studied. Immature oocytes from the initial to vitelogenic (diameter &lt;1.7 mm) and mature (diameter &gt;1.8 mm) stages of Steindachneridion parahybae were evaluated. Four distinct experiments were performed, three using immature oocytes and one using oocytes at the mature stage. For each oocyte stage, the best maintenance solution to be used: Hank or 50% L15 and; viability after baths for 30min (room temperature) at cryoprotectant concentrations ranging from 0.25 to 4M were evaluated. Different tests were used to evaluate oocyte viability: in vitro maturation followed by observation of germinal vesicle breakdown (only for immature oocytes), Trypan Blue staining (all stages) and fertilization and hatching rates (mature stage only). Results showed that the toxic effect of cryoprotectants on oocytes generally increases with increasing concentrations. Sensitivity of oocytes to cryoprotectants increases according to the stage of development, with mature oocytes being more sensitive. Sucrose, fructose, methanol, propylene glycol and dimethyl sulfoxide can be used as cryoprotectants for S. parahybae oocytes.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000600650
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/0103-8478cr20170667
Formato: | text/html
Fonte: | Ciência Rural  v.48 n.6 2018
Fonte: | info:eu-repo/semantics/openAccess