Data/hora: 02/06/2024 16:03:54

Provedor de dados: | 65
País: | Brazil
Título: | An improved method for genomic DNA extraction from strawberry leaves
Autores: | Nunes,Claudinéia Ferreira; Ferreira,Juliano Lino; Fernandes,Mônique Carolina Nunes; Breves,Sâmera de Souza; Generoso,Andressa Leal; Soares,Bárbara Dantas Fontes; Dias,Mário Sérgio Carvalho; Pasqual,Moacir; Borem,Aluízio; Cançado,Geraldo Magela de Almeida.
Data: | 2011-08-01
Ano: | 2011
Palavras-chave: | Fragaria ananassa; Genomic DNA; Polivinilpirrolidone; Lyophilization; PCR.
Resumo: | Several extraction methods of genomic DNA for identification and characterization of genetic diversity in different plant species are routinely applied during molecular analysis. However, the presence of undesirable compounds such as polyphenols and polysaccharides is one of the biggest problems faced during the isolation and purification of high quality DNA in plants. Therefore, achievement of fast and accurate methods for DNA extraction is crucial in order to produce pure samples. Leaves of strawberry genotypes (Fragaria ananassa) have high contents of polysaccharides and polyphenols which increase the sample viscosity and decrease the DNA quality, interfering with the PCR performance. Thereby, in this study we evaluated the quality and amount of genomic DNA extracted from young leaves of strawberry after tissue lyophilization and maceration in presence of polivinilpirrolidone (PVP). The CTAB method was used as reference procedure and it was modified to improve the DNA extraction. The modifications consisted of tissue lyophilization overnight until it was completely freeze-dried and addition of PVP during the tissue maceration in liquid nitrogen. The results showed the efficiency and reliability of the modified method compared to the unmodified method, indicating that combination of lyophilization and PVP improve the quality and amount of the DNA extracted from strawberry leaves.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000800014
Editor: | Universidade Federal de Santa Maria
Relação: | 10.1590/S0103-84782011000800014
Formato: | text/html
Fonte: | Ciência Rural  v.41 n.8 2011
Fonte: | info:eu-repo/semantics/openAccess