Data/hora: 02/06/2024 14:32:28

Provedor de dados: | 69
País: | Chile
Título: | Simple method to prepare DNA templates from a slice of peanut cotyledonary tissue for Polymerase Chain Reaction
Autores: | Yu,Shu Tao; Wang,Chuan Tang; Yu,Shan Lin; Wang,Xiu Zhen; Tang,Yue Yi; Chen,Dian Xu; Zhang,Jian Cheng.
Data: | 2010-07-01
Ano: | 2010
Palavras-chave: | Cotyledonary tissue; DNA extraction; Groundnut; PCR; Peanut.
Resumo: | An efficient DNA extraction method was developed for peanut seed, where only 3-5 mg cotyledonary tissue was enough for more than 50 PCR reactions with a reaction volume of 15 &amp;#956;l. Both low copy number and high copy number DNA sequences were successfully amplified. Processing one seed sample only took about half an hour. Sampling had no significant effects on germination and development. The DNA extraction method makes it possible to identify transformants and conduct molecular marker studies prior to sowing, and thus may greatly hasten research progress.
Tipo: | Journal article
Idioma: | Inglês
Identificador: | http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000400012
Editor: | Pontificia Universidad Católica de Valparaíso
Formato: | text/html
Fonte: | Electronic Journal of Biotechnology  v.13 n.4 2010