Data/hora: 03/06/2024 04:27:10

Provedor de dados: | 74
País: | Brazil
Título: | LNO3 AND L3 Are Associated With Antiproliferative And Pro-Apoptotic Action In Hepatoma Cells
Autores: | Zanelatto,Leonardo Campos; Silva,Patrícia Benites Gonçalves da; Sartori,Daniele; Panis,Carolina; Lepri,Sandra; Fátima,Ângelo de; Mantovani,Mário Sérgio.
Data: | 2016-06-01
Ano: | 2016
Palavras-chave: | Thalidomide analogues; Antiproliferative activity; Cytotoxicity; Free radicals; Apoptosis.
Resumo: | Abstract The identification of antitumoral substances is the focus of intense biomedical research. Two structural analogues of thalidomide, LNO3 and L3, are two synthetic compounds that might possess such antitumor properties. We evaluated the toxicological effects of these substances, including cytotoxicity, genotoxicity and induction of apoptosis in HTC cells. Additionally, the production of free radicals (nitric oxide and superoxide) was investigated, and the expression of caspases genes 3, 8, and 9 were determined by RT-qPCR. The compounds exhibited cytotoxic effects that resulted in inhibited cell proliferation. LNO3 showed to be more effective and toxic than L3 in all assays. LNO3 stimulated the release of NO and superoxide, which was accompanied by the formation of peroxynitrite. Apoptosis was induced in a dose-dependent manner by both compounds; however, the expression of caspases 3, 8 and 9 was unchanged. These results suggested that L3 and LNO3 possess antiproliferative and pro-apoptotic effects in HTC cells. Additionally, although they exhibited cytotoxicity, L3 and LNO3 might be useful coadjuvants in tumor treatment studies.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000200270
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/1678-4685-gmb-2015-0184
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.39 n.2 2016
Fonte: | info:eu-repo/semantics/openAccess