Data/hora: 03/06/2024 03:41:51

Provedor de dados: 	74	
País: 	Brazil	
Título: 	C-banding and fluorescent in situ hybridization with rDNA sequences in chromosomes of Cycloneda sanguinea Linnaeus (Coleoptera, Coccinellidae)	
Autores: 	Maffei,Eliane Mariza Dortas; Pompolo,Silvia das Graças; Petitpierre,Eduard.	
Data: 	2004-01-01	
Ano: 	2004	
Palavras-chave: 	In situ hybridization; RDNA sequences; Sequential FISH/Ag-NOR staining; C-banding; Cycloneda sanguinea.	
Resumo: 	The aim of this study was to describe mitotic and meiotic chromosomes of Cycloneda sanguinea using C-banding, fluorescent in situ hybridization (FISH) rDNA probes, and sequential FISH/Ag-NOR staining. The chromosome number was 2n = 18 + XX for females and 2n = 18 + Xy for males. The X chromosome was metacentric and the Y chromosome was very small. During meiosis, the karyotypic meioformula was n = 9 + Xy p, and sex chromosomes configured a parachute at metaphase I. At the beginning of pachytene, bivalents were still individualized, and sex chromosomes were associated end-to-end through the heteropycnotic region of the X chromosome. Later in pachytene, further condensation led to the formation of a pseudo-ring by the sex bivalent. All chromosomes showed pericentromeric heterochromatin. FISH and sequential FISH/Ag-NOR staining evidenced the location of the nucleolar organizer region in one pair of autosomes (at spermatogonial metaphase). During meiosis, these genes were mapped to a region outside the sex vesicle by FISH, although Xy p was deeply stained with silver at metaphase I. These results suggest that these argyrophilic substances are of a nucleolar protein nature, and seem to be synthesized by a pair of autosomes and imported during meiosis (prophase I) to the sex pair, during the association of the sex chromosomes.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572004000200011	
Editor: 	Sociedade Brasileira de Genética	
Relação: 	10.1590/S1415-47572004000200011	
Formato: 	text/html	
Fonte: 	Genetics and Molecular Biology  v.27 n.2 2004	
Fonte: 	info:eu-repo/semantics/openAccess