Data/hora: 13/06/2024 03:42:42

Provedor de dados: | 74
País: | Brazil
Título: | A porcine gene, PBK, differentially expressed in the longissimus muscle from Meishan and Large White pig
Autores: | Yonggang,Liu; Sumei,Zhao; Hongbin,Pan; Shizheng,Gao.
Data: | 2009-01-01
Ano: | 2009
Palavras-chave: | Pig; PBK; MRNA differential display; RACE.
Resumo: | An investigation of differences in gene expression in the longissimus muscle of Meishan and Large White pigs was undertaken, using the mRNA display technique. A fragment of one differentially expressed gene was isolated and sequenced, whereupon the complete cDNA sequence was then obtained by using the rapid amplification of cDNA ends (RACE). The nucleotide sequence of the gene is not related to any known porcine gene. Sequence analysis revealed that the open reading frame of this gene encodes a protein with 322 amino acids, thus displaying high sequence identity with the PDZ binding kinase (PBK) of eleven other animal species - dog, horse, cattle, human, chimpanzee, crab-eating macaque, rhesus monkey, rat, mouse, gray short-tailed opossum and platypus, so it can be defined as the porcine PBK gene. This gene was finally assigned GeneID:100141310. Phylogenetic tree analysis revealed that the swine PBK gene has a closer genetic relationship with the PBK gene of platypus. Gene expression analysis of eight tissues of a Meishan x Large White cross showed that the porcine PBK gene is differentially expressed in various tissues. Our experiment established the primary foundation for further research on this gene.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400017
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-47572009000400017
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.32 n.4 2009
Fonte: | info:eu-repo/semantics/openAccess