Data/hora: 03/06/2024 18:09:10

Provedor de dados: | 74
País: | Brazil
Título: | Chromosomal localization of microsatellite loci in Drosophila mediopunctata
Autores: | Cavasini,Renato; Batista,Marcos Roberto Dias; Klaczko,Louis Bernard.
Data: | 2015-03-01
Ano: | 2015
Palavras-chave: | Tripunctata; SSR; Genetic chromosomal analysis; BLAST search; Muller’s elements.
Resumo: | Drosophila mediopunctata has been used as a model organism for genetics and evolutionary studies in the last three decades. A linkage map with 48 microsatellite loci recently published for this species showed five syntenic groups, which had their homology determined to Drosophila melanogaster chromosomes. Then, by inference, each of the groups was associated with one of the five major chromosomes of D. mediopunctata. Our objective was to carry out a genetic (chromosomal) analysis to increase the number of available loci with known chromosomal location. We made a simultaneous analysis of visible mutant phenotypes and microsatellite genotypes in a backcross of a standard strain and a mutant strain, which had each major autosome marked. Hence, we could establish the chromosomal location of seventeen loci; including one from each of the five major linkage groups previously published, and twelve new loci. Our results were congruent with the previous location and they open new possibilities to future work integrating microsatellites, chromosomal inversions, and genetic determinants of physiological and morphological variation.
Tipo: | Info:eu-repo/semantics/report
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100055
Editor: | Sociedade Brasileira de Genética
Relação: | 10.1590/S1415-475738138120140275
Formato: | text/html
Fonte: | Genetics and Molecular Biology  v.38 n.1 2015
Fonte: | info:eu-repo/semantics/openAccess