Data/hora: 12/06/2024 13:46:05

Provedor de dados: | 79
País: | Brazil
Título: | Proteolytic activity of africanized honeybee (Apis mellifera: hymenoptera, apidae) venom
Autores: | LIMA,P. R. M. DE; BROCHETTO-BRAGA,M. R.; CHAUD-NETTO,J.
Data: | 2000-01-01
Ano: | 2000
Palavras-chave: | Venom; Africanized bee; Apis mellifera; Proteolytic activity; Gel filtration chromatography.
Resumo: | Some properties of a Africanized honeybee venom proteases were determined by enzymatic assays in solution, electrophoresis in SDS-PAGE, and gel filtration. Bee venom extracts were obtained by reservoir disruption, selective dialysis (cut off 12 kDa) to eliminate small components, such as the protease inhibitor present in the venom, and then fractionation of the dialyzed extract by gel filtration on a Sephadex G-100 column. The optimal conditions for the caseinolytic assays were pH 9.5, 2-hour digestion at 37 °C, and 1% casein concentration. The proteolytic activity was also determined by electrophoresis in SDS-PAGE with co-polymerized gelatin with three major bands of 66.0, 41.6, and 25.1 kDa. A principal serine-protease-like mechanism was revealed in the enriched fraction of proteolytic activity.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79302000000100004
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos - CEVAP, Universidade Estadual Paulista - UNESP
Relação: | 10.1590/S0104-79302000000100004
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins  v.6 n.1 2000
Fonte: | info:eu-repo/semantics/openAccess