Data/hora: 03/06/2024 11:43:45

Provedor de dados: | 80
País: | Brazil
Título: | A novel Kv1.1 potassium channel blocking toxin from the venom of Palamneus gravimanus (Indian black scorpion)
Autores: | More,S. S.; Mirajkar,K. K.; Gadag,J. R.; Menon,K. S.; Mathew,M. K.
Data: | 2005-09-01
Ano: | 2005
Palavras-chave: | HKv1.1; K+ channel; PGT; TEVC; Palamneus gravimanus; Xenopus laevis oocytes; Indian black scorpion.
Resumo: | A peptide toxin was isolated from the venom of Palamneus gravimanus, the Indian black scorpion, to block human Kv1.1 channels expressed in Xenopus laevis oocytes. A 4.5 kD peptide (toxin), as confirmed by SDS-PAGE, was purified to homogeneity by ion exchange chromatography using CM-Sephadex C-25 followed by Sephadex G-50 gel filtration. Palamneus gravimanus toxin (PGT) selectively blocks the human cloned voltage-gated potassium channel hKv1.1 in a two-electrode voltage-clamp (TEVC) technique. The results obtained indicate that the toxin blocks the hKv1.1 channel at a nanomolar concentration range (Ki value of 10 nM) of the peptide to the external side of the cell. The blockage seems to be voltage-dependent. Comparative structure of PGT (a 4.5 kD peptide) with BTK-2 suggests a close relationship; therefore this toxin can be employed to investigate the hKv1.1 channel structure.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000300009
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992005000300009
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.11 n.3 2005
Fonte: | info:eu-repo/semantics/openAccess