Data/hora: 13/06/2024 08:55:44

Provedor de dados: 	80	
País: 	Brazil	
Título: 	Crotamine in Crotalus durissus: distribution according to subspecies and geographic origin, in captivity or nature	
Autores: 	Tasima,Lídia J.; Serino-Silva,Caroline; Hatakeyama,Daniela M.; Nishiduka,Erika S.; Tashima,Alexandre K.; Sant’Anna,Sávio S.; Grego,Kathleen F.; Morais-Zani,Karen de; Tanaka-Azevedo,Anita M.	
Data: 	2020-01-01	
Ano: 	2020	
Palavras-chave: 	Rattlesnake; Snake venom; Toxins; Venom variation; Antivenom.	
Resumo: 	Abstract  Background: Crotalus durissus is considered one of the most important species of venomous snakes in Brazil, due to the high mortality of its snakebites. The venom of Crotalus durissus contains four main toxins: crotoxin, convulxin, gyroxin and crotamine. Venoms can vary in their crotamine content, being crotamine-negative or -positive. This heterogeneity is of great importance for producing antivenom, due to their different mechanisms of action. The possibility that antivenom produced by Butantan Institute might have a different immunorecognition capacity between crotamine-negative and crotamine-positive C. durissus venoms instigated us to investigate the differences between these two venom groups.  Methods: The presence of crotamine was analyzed by SDS-PAGE, western blotting and ELISA, whereas comparison between the two types of venoms was carried out through HPLC, mass spectrometry analysis as well as assessment of antivenom lethality and efficacy.  Results: The results showed a variation in the presence of crotamine among the subspecies and the geographic origin of snakes from nature, but not in captive snakes. Regarding differences between crotamine-positive and -negative venoms, some exclusive proteins are found in each pool and the crotamine-negative pool presented more phospholipase A2 than crotamine-positive pool. This variation could affect the time to death, but the lethal and effective dose were not affected.  Conclusion: These differences between venom pools indicate the importance of using both, crotamine-positive and crotamine-negative venoms, to produce the antivenom.	
Tipo: 	Info:eu-repo/semantics/article	
Idioma: 	Inglês	
Identificador: 	http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992020000100307	
Editor: 	Centro de Estudos de Venenos e Animais Peçonhentos	
Relação: 	10.1590/1678-9199-jvatitd-2019-0053	
Formato: 	text/html	
Fonte: 	Journal of Venomous Animals and Toxins including Tropical Diseases  v.26 2020	
Fonte: 	info:eu-repo/semantics/openAccess