Data/hora: 12/06/2024 14:21:16

Provedor de dados: | 80
País: | Brazil
Título: | A glycoprotein with anti-inflammatory properties secreted by an Aspergillus nidulans modified strain
Autores: | Queiroz,J. C. F.; Teixeira,C. F. P.; Lebrun,I.; Spencer,P. J.; Zucchi,T. M. A. D.
Data: | 2007-01-01
Ano: | 2007
Palavras-chave: | Aspergillus nidulans; Protein purification and characterization; Glycoprotein; Anti-inflammatory activity.
Resumo: | Total RNA from lipopolysaccharide (LPS)-stimulated rat macrophages used to treat protoplasts from an Aspergillus nidulans strain originated the RT2 regenerated strain, whose culture supernatant showed anti-inflammatory activity in Wistar rats. The protein fraction presenting such anti-inflammatory activity was purified and biochemically identified. The screening of the fraction responsible for such anti-inflammatory property was performed by evaluating the inhibition of carrageenan-induced paw edema in male Swiss mice. Biochemical analyses of the anti-inflammatory protein used chromatography, carbohydrates quantification of the protein sample, amino acids content analysis and sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE). Total sugar quantification revealed 32% glycosylation of the protein fraction. Amino acid analysis of such fraction showed a peculiar pattern presenting 29% valine. SDS-PAGE revealed that the protein sample is pure and its molecular weight is about 40kDa. Intravenous injection of the isolated substance into mice significantly inhibited carrageenan-induced paw edema. The isolated glycoprotein decreased carrageenan-induced paw edema in a prostaglandin-dependent phase, suggesting an inhibitory effect of the isolated glycoprotein on prostaglandin synthesis.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000200010
Editor: | Centro de Estudos de Venenos e Animais Peçonhentos
Relação: | 10.1590/S1678-91992007000200010
Formato: | text/html
Fonte: | Journal of Venomous Animals and Toxins including Tropical Diseases  v.13 n.2 2007
Fonte: | info:eu-repo/semantics/openAccess