Data/hora: 10/06/2024 13:46:10

Provedor de dados: | 82
País: | Brazil
Título: | Morphological regional differences of epithelial cells along the midgut in Diatraea saccharalis Fabricius (Lepidoptera: Crambidae) larvae
Autores: | Pinheiro,Daniela O.; Quagio-Grassiotto,Irani; Gregório,Elisa A.
Data: | 2008-08-01
Ano: | 2008
Palavras-chave: | Morphology; Sugarcane borer; Histochemistry; Scanning electron microscopy; Light microscopy.
Resumo: | The sugarcane borer, Diatraea saccharalis Fabricius, is a pest to sugarcane and many other crops. This work aims to characterize morphological variability in the epithelial cells (columnar, goblet and regenerative) along the midgut of D. saccharalis larvae. Fragments of the midgut (anterior, middle and posterior regions) were fixed and processed by light and scanning electron microscopy. There are both cytochemical and ultrastructural differences in the morphology of the epithelial cells, depending on their localization along the midgut. The apical surface of columnar cells shows an increase in both number and size of the apical protrusions from the anterior to the posterior midgut regions. There is an increase in the amount of PAS-positive (Periodic Acid-Schiff Reaction) granules detected in the cytoplasm of both the columnar and regenerative cells, from the anterior to the posterior region. The goblet cell apical surface is narrow in the anterior region, and enlarged in the posterior midgut; the chamber's cytoplasm extrusion are small and thin at the apical cavity surface, being thicker, longer and more numerous at the basal portion of the cavity. Our results suggest that the sugarcane borer midgut has two morphologically different regions, the anterior and the posterior; the middle region is a transitional region.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2008000400009
Editor: | Sociedade Entomológica do Brasil
Relação: | 10.1590/S1519-566X2008000400009
Formato: | text/html
Fonte: | Neotropical Entomology  v.37 n.4 2008
Fonte: | info:eu-repo/semantics/openAccess