Data/hora: 01/06/2024 02:49:48

Provedor de dados: | 86
País: | Brazil
Título: | Bioassay for detection of transgenic soybean seeds tolerant to glyphosate
Autores: | Torres,Antonio Carlos; Nascimento,Warley Marco; Paiva,Sonia Alessandra Vasconcelos; Aragão,Fernando Antonio Souza de.
Data: | 2003-09-01
Ano: | 2003
Palavras-chave: | Glycine max; Herbicide resistance; Transgenic plants; Biosynthesis.
Resumo: | Glyphosate is a systemic, nonselective, postemergence herbicide that inhibits growth of both weeds and crop plants. Once inside the plant, glyphosate interferes with biosynthesis of aromatic amino acids phenylalanine, tyrosine, and tryptophan, by inhibiting the activity of 5enolpyruvylshikimate-3-phosphate synthase (EPSPS), a key enzyme of the shikimate pathway. The objective of this work was to develop a simple, effective and inexpensible method for identification of transgenic soybean tolerant to glyphosate. This technique consisted in germinating soybean seeds in filter paper moistened with 100 to 200 muM of glyphosate. Transgenic soybean seeds tolerant to glyphosate germinated normally in this solution and, between 7 and 10 days, started to develop a primary root system. However non-transgenic seeds stopped primary root growth and emission of secondary roots.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2003000900005

Editor:

Embrapa Secretaria de Pesquisa e Desenvolvimento

Pesquisa Agropecuária Brasileira

Relação: | 10.1590/S0100-204X2003000900005
Formato: | text/html
Fonte: | Pesquisa Agropecuária Brasileira  v.38 n.9 2003
Fonte: | info:eu-repo/semantics/openAccess