Data/hora: 10/06/2024 07:58:02

Provedor de dados: | 88
País: | Brazil
Título: | Comparative uptake and metabolism of 2-[14C]-2,4-dichlorophenoxyacetic acid in callus cultures of monocot (Dioscorea spp.) and dicot (Nicotiana tabacum L.) plants
Autores: | Viana,Ana Maria; Mantell,Sinclair Hugh.
Data: | 1998-04-01
Ano: | 1998
Palavras-chave: | Dioscorea spp. Nicotiana tabacum plant tissue culture 2; 4-dichlorophenoxyacetic acid (2; 4-D)-uptake auxin metabolism radioactively labelled 2; 4-D.
Resumo: | (Comparative uptake and metabolism of 2-[14C]-2,4-dichlorophenoxyacetic acid in callus cultures of monocot (Dioscorea spp.) and dicot (Nicotiana tabacum L.) plants). The uptake and metabolism of 2-[14C]-2,4-dichlorophenoxyacetic acid (2,4-D) were investigated in leaf calluses of Nicotiana tabacum, tuber calluses of Dioscorea opposita and calluses derived from zygotic embryos, leaves and petioles of Dioscorea composita. Striking similarities were evident in the patterns of 2,4-D metabolites and their chemical characteristics in the three callus types of D. composita compared, but significant differences were detected among the patterns of rnetabolites in the three species studied. Preliminary investigations on the stability of various metabolites (separated using TLC) by hydrolysis showed that sugar esters appeared to be the major metabolites in tobacco whilst in yams (D. opposita) glycosides were shown to be the main ones, which indicated a similarity between plants of Gramineae and Dioscoreaceae in terms of 2,4-D metabolism. Release of 2,4-D from tobacco callus cells upon their transfer to 2,4-D-free medium was detected and the implications of this are discussed in relation to the cultural conditions necessary to induce morphogenesis in vitro.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84041998000100009
Editor: | Sociedade Botânica de São Paulo
Relação: | 10.1590/S0100-84041998000100009
Formato: | text/html
Fonte: | Brazilian Journal of Botany  v.21 n.1 1998
Fonte: | info:eu-repo/semantics/openAccess