Data/hora: 04/06/2024 14:26:27

Provedor de dados: | 96
País: | Brazil
Título: | Artificial fertilization of oocytes and sperm activation in pacu: effects of the spermatozoa:oocyte ratio, water volume, and in natura semen preservation
Autores: | Sanches,Eduardo Antônio; Baggio,Diego Mendes; Piana,Pitágoras Augusto; Souza,Bruno Estevão de; Bombardelli,Robie Allan.
Data: | 2011-01-01
Ano: | 2011
Palavras-chave: | Fish; Gametes; Insemination doses; Myleinae; Reproduction.
Resumo: | The objective of this work was to investigate artificial fertilization and the duration of sperm motility in pacu with different insemination doses, water volume, and in natura semen preservation. It was carried out four experimentsfor evaluation of insemination doses (7x10³, 7x10(4), 7x10(5), 7x10(6), and 7x10(7) spermatozoa oocytes-1) on the artificial fertilization of oocytes; the effect of water volume (0.5, 15.0, 30.0, 45.0, and 60.0 mL water mL-1 of oocyte) with insemination doses of 105,481 and 210,963 spermatozoa oocytes-1; the effect of semen dilutions (0.005, 0.05, 0.5, and 5.0 µL semen mL-1 of water) on sperm motility duration; and the effect of storage at 15ºC for 9h on sperm motility duration and sperm survival ratio. The highest results obtained were: insemination doses from 7x10³ to 7x10(7) spermatozoa oocytes-1; from 15 to 60mL water mL-1 of oocytes; semen dilution of 0.005 µL semen/mL water and 98.65% sperm survival until 2h45min 36s preservation time. Preservation at 15ºC for 9h does not influence sperm motility duration. The highest fertilization rates can be observed by using 0.27 to 270 µL semen mL-1 of oocytes with 15 at 60 mL water for activation.
Tipo: | Info:eu-repo/semantics/article
Idioma: | Inglês
Identificador: | http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011000100001
Editor: | Sociedade Brasileira de Zootecnia
Relação: | 10.1590/S1516-35982011000100001
Formato: | text/html
Fonte: | Revista Brasileira de Zootecnia  v.40 n.1 2011
Fonte: | info:eu-repo/semantics/openAccess