| Registro completo |
| Provedor de dados: |
Agronomy
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| País: |
Brazil
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| Título: |
Developing an in vitro optimized protocol to sweet potato landraces conservation
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| Autores: |
Vettorazzi, Renato Gobbi
Carvalho, Virginia Silva
Sudré, Cláudia Pombo
Rodrigues, Rosana
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| Data: |
2017-05-17
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| Ano: |
2017
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| Palavras-chave: |
5.01.03.00-8 Ipomoea batatas
Minimal growth
Germplasm bank
Maintenance of diversity
Fitotecnia
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| Resumo: |
This study aimed to develop a protocol for the in vitro conservation of 30 accessions of sweet potato collected in the northern region of Rio de Janeiro State, Brazil, aiming to extend the period between subcultures for this species. An in vitro minimal growth experiment was established with accession UENF 1931 in a factorial arrangement consisting of four concentrations of mineral salts in MS medium and four concentrations of sucrose, at two temperatures. The others accessions were grown in medium containing 100% MS salts and 2% sucrose, which was the medium that allowed the in vitro conservation of accession UENF 1931 for a longer time period. For this second experiment, 30 accessions of sweet potato and two temperatures were used. Plant height, number of leaves, and survival rate were measured every 30 days for 12 months, in both experiments. In the last stage, the sweet potato plantlets originating from in vitro minimal growth were acclimatized and exhibited 100% survival. The recommended in vitro minimal growth conditions for the studied accessions are MS medium with 100% concentration of mineral salts and 2% sucrose, at a temperature of 27±2°C, with subculture performed every 180 days.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32700
10.4025/actasciagron.v39i3.32700
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| Editor: |
Universidade Estadual de Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32700/pdf
http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/downloadSuppFile/32700/12899
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Agronomy; v. 39, n. 3 (2017); 359-367
Acta Scientiarum. Agronomy; v. 39, n. 3 (2017); 359-367
1807-8621
1679-9275
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| Direitos: |
Direitos autorais 2017 Acta Scientiarum. Agronomy
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