| Registro completo |
| Provedor de dados: |
Biological Sciences
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| País: |
Brazil
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| Título: |
Analytical methods for evaluation of the fatty acid metabolism in rat liver
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| Autores: |
Gilglioni, Eduardo Hideo
Campos-Shimada, Lilian Brites
Martins Maciel, Elismari Rizato
Ishii-Iwamoto, Emy Luiza
Salgueiro-Pagadigorria, Clairce Luzia
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| Data: |
2018-11-30
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| Ano: |
2018
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| Palavras-chave: |
2.08.03.00-1 Metabolismo e Bioenergética liver
Fatty acid/metabolism
Fatty liver
Hepatic steatosis. Bioquímica
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| Resumo: |
The liver is an essential organ for body energy homeostasis, controlling the biosynthesis, uptake and the disposal of carbohydrates and lipids. The hepatic steatosis is a common condition frequently associated with metabolic diseases and is characterized by the excessive accumulation of triglycerides in the liver. In recent years, many efforts have been devoted to prevent and treat the hepatic steatosis, but it remains being pointed out as the major cause for chronic hepatic diseases in Western countries. A considerable part of the knowledge about the physiopathology of hepatic steatosis, the effects of diets and drugs on the metabolic capacity of the liver to metabolize fatty acids, as well as the potential therapeutic approaches for hepatic steatosis derived from experimental animal models using rodents. Here, in this article, we present the details of some of the most common techniques used to evaluate fatty acid metabolism in liver of rats, including quantification of total lipid content, measurement of fatty acid oxidation in isolated subcellular fractions and procedures to measure the activities of important lipogenic enzymes. Classical protocols previously described to be performed using samples from other tissues were adapted to liver samples and different techniques with equivalent aims were compared. The principles and the advantages in terms of reliability and costs were discussed and the procedures here described can be applied for a low-cost broad evaluation of the fatty acid metabolism in liver of rats submitted to different experimental conditions.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40040
10.4025/actascibiolsci.v40i1.40040
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| Editor: |
Universidade Estadual De Maringá
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| Relação: |
http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/40040/pdf
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| Formato: |
application/pdf
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| Fonte: |
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e40040
Acta Scientiarum. Biological Sciences; v. 40 (2018): Publicação Contínua; e40040
1807-863X
1679-9283
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| Direitos: |
Direitos autorais 2018 Acta Scientiarum. Biological Sciences
http://creativecommons.org/licenses/by/4.0
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