| Registro completo |
| Provedor de dados: |
ArchiMer
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| País: |
France
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| Título: |
DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
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| Autores: |
Lazerges, M.
Perrot, H.
Rabehagasoa, N.
Compere, Chantal
Dreanno, Catherine
Mucio Pedroso, M.
Faria, R. C.
Bueno, P. R.
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| Data: |
2012-08
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| Ano: |
2012
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| Palavras-chave: |
Biosensors
DNA
Hybridization kinetics
Quartz crystal microbalance
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| Resumo: |
The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and increases with DNA target length. It was established that k(2) (s(-1)), relevant to irreversible formation of a stable duplex, varies in an opposite way to K-1 with DNA target length. (C) 2012 Published by Elsevier B.V.
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| Tipo: |
Text
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| Idioma: |
Inglês
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| Identificador: |
http://archimer.ifremer.fr/doc/00101/21195/19415.pdf
DOI:10.1016/j.snb.2012.05.023
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| Editor: |
Elsevier Science Sa
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| Relação: |
http://archimer.ifremer.fr/doc/00101/21195/
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| Formato: |
application/pdf
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| Fonte: |
Sensors And Actuators B-chemical (0925-4005) (Elsevier Science Sa), 2012-08 , Vol. 171 , P. 522-527
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| Direitos: |
2012 Published by Elsevier B.V.
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