| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Plant or fungal sequences? An alternative optimized PCR protocol to avoid ITS (nrDNA) misamplification
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| Autores: |
Miranda,Vitor Fernandes Oliveira de
Martins,Vanderlei Geraldo
Furlan,Antonio
Bacci Jr.,Maurício
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| Data: |
2010-02-01
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| Ano: |
2010
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| Palavras-chave: |
Polymerase
DNA
Drosera
Fungi
Phylogeny
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| Resumo: |
The nuclear ribosomal DNA internal transcribed spacers (ITS1 and ITS2) from leaves of Drosera (Droseraceae) were amplified using "universal" primers. The analysis of the products demonstrated most samples were a molecular mixture as a result of unsuccessful and non-specific amplifications. Among the obtained sequences, two were from Basidiomycota fungi. Homologous sequences of Basidiomycota were obtained from GenBank database and added to a data set with sequences from Drosera leaves. Parsimony analysis demonstrated that one sequence was amplified from an Ustilaginomycetes fungus, and another from a Heterobasidiomycetes. Possibly these fungi were associated to leaves of Drosera, and not because of samples contamination. In order to provide optimization and a better specificity of PCR (polymerase chain reaction), a very successful method was demonstrated using dimethyl sulfoxide (DMSO) and bovine serum albumin (BSA) in reactions.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100018
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132010000100018
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.53 n.1 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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