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Provedor de dados:  BABT
País:  Brazil
Título:  Degradation of monoaromatics by Bacillus pumilus MVSV3
Autores:  Surendra,Sheeba Varma
Mahalingam,Brinda Lakshmi
Velan,Manickam
Data:  2017-01-01
Ano:  2017
Palavras-chave:  Bacillus pumilus MVSV3
Catechol 1
2-dioxygenase
Ortho pathway
BTEX.
Resumo:  ABSTRACT Monoaromatics, such as benzene, toluene, ethylbenzene and xylene (BTEX), are simple aromatic compound that are highly toxic due to their high solubility nature. Many chemical and physical methods for their degradation and breakup into nontoxic products are available, but still use of microorganism is preferred over these processes. In this present study Bacillus pumilus MVSV3 (Accession number JN089707), a less explored bacteria in the field of BTEX degradation, isolated from petroleum contaminated soil is utilized for BTEX degradation. At optimized conditions the isolate degraded 150 mg/L of BTEX completely within 48 h. GC-MS analysis revealed that the microorganism produces catechol and muconic acid during degradation indicating an ortho pathway of degradation. Enzyme assays were carried out to identify and characterize catechol 1, 2- dioxygenase (C12D). The optimal temperature and pH for the enzyme activity was identified as 35 (C and 7.5, respectively. SDS-PAGE revealed the molecular weight of the enzyme to be approximately 35,000 Da. Zymography analysis indicated the presence of three isoforms of the enzyme. Hence Bacillus pumilus MVSV3 and the isolated C12D, proved to be efficient in degrading the toxic aromatic compounds.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132017000100408
Editor:  Instituto de Tecnologia do Paraná - Tecpar
Relação:  10.1590/1678-4324-2017160319
Formato:  text/html
Fonte:  Brazilian Archives of Biology and Technology v.60 2017
Direitos:  info:eu-repo/semantics/openAccess
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