| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Scale up of dextran production from a mutant of Pediococcus pentosaceus (SPAm) using optimized medium in a bioreactor
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| Autores: |
Patel,Seema
Kothari,Damini
Shukla,Rishikesh
Das,Debasish
Goyal,Arun
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| Data: |
2011-12-01
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| Ano: |
2011
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| Palavras-chave: |
Pediococcus pentosaceus
Dextran
Plackett-Burman Design
Central Composite Design
Response Surface Methodology
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| Resumo: |
The mutant of Pediococcus pentosaceus (SPAm) produced earlier by UV-mutagenesis exhibiting higher dextransucrase activity as compared to wild-type was used. The generated mutant SPAm gave 12.2 mg/ml, a 20% higher dextran than wild-type. Response surface methodology was carried out for further enhancement of dextran production. To enhance dextran production by the mutant SPAm, Plackett-Burman Design and a 2² full factorial Central Composite Design was employed. After response optimization, the optimum concentration of sucrose and yeast extract was 5.115% (w/v) and 0.635% (w/v), respectively. The experimental values of dextran 36.0 mg/ml at flask level and 35.0 mg/ml at bioreactor level were in good agreement with the predicted value of 40.8 mg/ml. The increase in dextran production by the mutant SPAm using the optimized medium was 3 fold higher as compared to unoptimized medium.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000600007
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132011000600007
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.54 n.6 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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