| Registro completo |
| Provedor de dados: |
BABT
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| País: |
Brazil
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| Título: |
Jacaratia corumbensis O. Kuntze a new vegetable source for milk-clotting enzymes
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| Autores: |
Duarte,Ana Rodrigues
Duarte,Débora Maria Rodrigues
Moreira,Keila Aparecida
Cavalcanti,Maria Taciana Holanda
Lima-Filho,José Luiz de
Porto,Ana Lúcia Figueiredo
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| Data: |
2009-02-01
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| Ano: |
2009
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| Palavras-chave: |
Jacaratia corumbensis O. kuntze
Milk clotting enzyme
Characterization
Purification
Vegetable enzyme
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| Resumo: |
The partial characterization and purification of milk clotting enzyme obtained from the (root latex) of Jacaratia corumbensis O. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. The ammonium sulphate precipitate showed five fractions (AS1- 0-20%; AS2 - 20-40%; AS3 - 40-60%; AS4 - 60-80%; AS5 - 80-100%) and among the fractions obtained, the 40-60% fraction (AS3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. This fraction when applied on Mono Q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. The optimal pH for the crude and partially purified extract was 6.5 and 7.0, respectively. The maximum milk-clotting activity was at 55ºC for the both crude and partially purified extracts. The enzyme was inhibited by iodoacetic acid which suggested that this enzyme was a cysteine protease, with molecular weight of 33 kDa.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100001
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| Editor: |
Instituto de Tecnologia do Paraná - Tecpar
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| Relação: |
10.1590/S1516-89132009000100001
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| Formato: |
text/html
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| Fonte: |
Brazilian Archives of Biology and Technology v.52 n.1 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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