| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Xylanase production by Aspergillus awamori under solid state fermentation conditions on tomato pomace
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| Autores: |
Umsza-Guez,Marcelo A.
Díaz,Ana B.
Ory,Ignacio de
Blandino,Ana
Gomes,Eleni
Caro,Ildefonso
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| Data: |
2011-12-01
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| Ano: |
2011
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| Palavras-chave: |
Solid state fermentation (SSF)
Tomato pomace
Hydrolytic enzymes
Plate-type bioreactor
Xylanase production
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| Resumo: |
In this work, tomato pomace, a waste abundantly available in the Mediterranean and other temperate climates agro-food industries, has been used as raw material for the production of some hydrolytic enzymes, including xylanase, exo-polygalacturonase (exo-PG), cellulase (CMCase) and α-amylase. The principal step of the process is the solid state fermentation (SSF) of this residue by Aspergillus awamori. In several laboratory experiments, maximum xylanase and exo-PG activities were measured during the first days of culture, reaching values around 100 and 80 IU/gds (international units of enzyme activity per gram of dried solid), respectively. For CMCase and α-amylase production remained almost constant along fermentation, with average values of 19 and 21.5 IU/gds, respectively. Experiments carried out in a plate-type bioreactor at lab scale showed a clear positive effect of aeration on xylanase and CMCase, while the opposite was observed for exo-PG and α-amylase. In general, xylanase was the enzyme produced in higher levels, thus the optimum conditions for the determination of the enzyme activity was characterized. The xylanase activity shows an optimum pH of 5 and an optimum temperature of 50 ºC. The enzyme is activated by Mg2+, but strongly inhibited by Hg2+ and Cu2+. The enzymatic activity remains quite high if the extract is preserved in a range of pH from 3 to 10 and a temperature between 30 ºC to 40 ºC.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400046
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822011000400046
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.42 n.4 2011
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| Direitos: |
info:eu-repo/semantics/openAccess
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