| Registro completo |
| Provedor de dados: |
BJM
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| País: |
Brazil
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| Título: |
Identification of Lactobacillus plantarum, Lactobacillus pentosus and Lactobacillus fermentum from honey stomach of honeybee
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| Autores: |
Tajabadi,Naser
Mardan,Makhdzir
Saari,Nazamid
Mustafa,Shuhaimi
Bahreini,Rasoul
Manap,Mohd Yazid Abdul
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| Data: |
2013-09-01
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| Ano: |
2013
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| Palavras-chave: |
Honey bee
Honey stomach
Lactobacillus
Probiotic
16S rDNA gene sequences
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| Resumo: |
This study aimed to isolate and identify Lactobacillus in the honey stomach of honeybee Apis dorsata. Samples of honeybee were collected from A. dorsata colonies in different bee trees and Lactobacillus bacteria isolated from honey stomachs. Ninety two isolates were Gram-stained and tested for catalase reaction. By using bacterial universal primers, the 16S rDNA gene from DNA of bacterial colonies amplified with polymerase chain reaction (PCR). Forty-nine bacterial 16S rDNA gene were sequenced and entrusted in GenBank. Phylogenetic analysis showed they were different phylotypes of Lactobacillus. Two of them were most closely relevant to the previously described species Lactobacillus plantarum. Other two phylotypes were identified to be closely related to Lactobacillus pentosus. However, only one phylotype was found to be distantly linked to the Lactobacillus fermentum. The outcomes of the present study indicated that L. plantarum, L. pentosus, and L. fermentum were the dominant lactobacilli in the honey stomach of honeybee A. dorsata collected during the dry season from Malaysia forest area - specifically "Melaleuca in Terengganu".
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300008
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| Editor: |
Sociedade Brasileira de Microbiologia
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| Relação: |
10.1590/S1517-83822013000300008
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| Formato: |
text/html
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| Fonte: |
Brazilian Journal of Microbiology v.44 n.3 2013
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| Direitos: |
info:eu-repo/semantics/openAccess
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