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Provedor de dados:  BJM
País:  Brazil
Título:  The improvement of bioactive secondary metabolites accumulation in Rumex gmelini Turcz through co-culture with endophytic fungi
Autores:  Ding,Chang-hong
Wang,Qian-Bo
Guo,Shenglei
Wang,Zhen-yue
Data:  2018-06-01
Ano:  2018
Palavras-chave:  Rumex gmelini Turcz (RGT)
Endophytic fungi
Aspergillus sp.
Seedlings
Bioactive secondary metabolites
Resumo:  Abstract Aspergillus sp., Fusarium sp., and Ramularia sp. were endophytic fungi isolated from Rumex gmelini Turcz (RGT), all of these three strains could produce some similar bioactive secondary metabolites of their host. However the ability to produce active components degraded significantly after cultured these fungi alone for a long time, and were difficult to recover. In order to obtain more bioactive secondary metabolites, the co-culture of tissue culture seedlings of RGT and its endophytic fungi were established respectively, and RGT seedling was selected as producer. Among these fungi, Aspergillus sp. showed the most significant enhancement on bioactive components accumulation in RGT seedlings. When inoculated Aspergillus sp. spores into media of RGT seedlings that had taken root for 20 d, and made spore concentration in co-culture medium was 1 × 104 mL-1, after co-cultured for 12 d, the yield of chrysophaein, resveratrol, chrysophanol, emodin and physcion were 3.52-, 3.70-, 3.60-, 4.25-, 3.85-fold of the control group. The extreme value of musizin yield was 0.289 mg, which was not detected in the control groups. The results indicated that co-culture with endophytic fungi could significantly enhance bioactive secondary metabolites production of RGT seedlings.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200362
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1016/j.bjm.2017.04.013
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.49 n.2 2018
Direitos:  info:eu-repo/semantics/openAccess
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