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Provedor de dados:  BJM
País:  Brazil
Título:  Evaluation of the Diramic system for urine cultures
Autores:  Higuchi,Cristian Kendi
Corrêa,Carlos Roberto
Rall,Vera Lucia Mores
Fernandes Junior,Ary
Data:  2006-09-01
Ano:  2006
Palavras-chave:  Diramic
Calibrated loop
Urinary tract infection
Urine cultures
Resumo:  The use of the Diramic system in microbiological diagnosis of urinary tract infections (UTI) was evaluated. This system was developed at the National Center for Scientific Research of Cuba, and it reads turbidimetric changes of microbial growth in culture media at 37ºC incubated for 4 hours. A total of 396 urine specimens were tested in the Laboratory of Microbiology of the School of Medicine - UNESP, Brazil using the Diramic system and the counting of colony forming units per urine millimeter (calibrated loop) as the reference method. The coincidence rate between the two methods was 96.46% (382 urine samples), and the differences in results were not significant (p<0.05). Sensitivity and specificity rates were 84.37% and 98.80%, respectively. False negative and false positive rates were 2.50% and 1.01%, respectively. The microorganisms isolated from positive urines were: Escherichia coli (68.75%); Klebsiella pneumoniae (10.94%); yeast (6.25%); Pseudomonas aeruginosa (4.69%); Enterobacter cloacae (3.12%); Proteus mirabilis, coagulase negative Staphylococcus, Morganella morganii, and Citrobacter freundii (1.56% each). The Diramic system was effective as screening method for urine cultures, however restrictions in the UTI diagnosis caused by yeasts and patients undergoing antibiotic therapy were negative characteristics of the system.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300005
Editor:  Sociedade Brasileira de Microbiologia
Relação:  10.1590/S1517-83822006000300005
Formato:  text/html
Fonte:  Brazilian Journal of Microbiology v.37 n.3 2006
Direitos:  info:eu-repo/semantics/openAccess
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