| Registro completo |
| Provedor de dados: |
Scientia Agricola
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| País: |
Brazil
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| Título: |
Marker assisted identification of tospovirus resistant tomato genotypes in segregating progenies
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| Autores: |
Nascimento,Ildon Rodrigues do
Maluf,Wilson Roberto
Figueira,Antônia Reis
Menezes,Cícero Beserra
Resende,Juliano Tadeu Vilela de
Faria,Marcos Ventura
Nogueira,Douglas Willian
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| Data: |
2009-06-01
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| Ano: |
2009
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| Palavras-chave: |
Lycopersicon esculentum
Sw-5
Vírus resistance
Molecular marker
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| Resumo: |
The SCAR (Sequence Characterized Amplified Region) 'Sw-421' molecular marker is located at 1.0 cM from the Sw-5 allele, originated from Lycopersicon peruvianum (L.), which confers resistance to the tomato spotted wilt virus (TSWV). However, it had not been tested yet in advanced tomato populations. The goal of this study was to distinguish resistant homozygotes (Sw-5/Sw-5) and heterozygotes (Sw-5/Sw-5+) from susceptible (Sw-5+/Sw-5+) plants in crossing populations with the Stevens cultivar and advanced backcrossing populations by using 'Sw421' SCAR marker. The amplification of 940 bp and 900 bp bands characterized the resistant homozygotes and susceptible controls, respectively. A two band pattern (900 bp and 940 bp) was observed in heterozygote genotypes (Sw-5/Sw-5+), which confirmed the co-dominant inheritance mechanism of the marker. Fifty seven plants from the isogenic progenies were characterized based on bands pattern: 18 plants (31.6%) were identified as resistant homozygotes, 8 plants (14.0%) as resistant heterozygotes and 31 plants (54.4%) were characterized as susceptible. The SCAR 'Sw-421' marker is an important tool for selection and pyramid resistance alleles, mainly when other resistance sources to the TSWV are available, such as the Rey de los Tempranos source.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162009000300003
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| Editor: |
São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
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| Relação: |
10.1590/S0103-90162009000300003
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| Formato: |
text/html
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| Fonte: |
Scientia Agricola v.66 n.3 2009
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| Direitos: |
info:eu-repo/semantics/openAccess
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