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Provedor de dados:  Scientia Agricola
País:  Brazil
Título:  Molecular identification of Aspergillus spp. isolated from coffee beans
Autores:  Magnani,Marciane
Fernandes,Thiago
Prete,Cássio Egidio Cavenaghi
Homechim,Martin
Ono,Elisabete Yurie Sataque
Vilas-Boas,Laurival Antonio
Sartori,Daniele
Furlaneto,Márcia Cristina
Fungaro,Maria Helena Pelegrinelli
Data:  2005-01-01
Ano:  2005
Palavras-chave:  ITS
Ribosomal DNA
Ochratoxin
Mycotoxin
Resumo:  Some species belonging to the genus Aspergillus are potential producers of ochratoxin A (OA), a mycotoxin with nephrotoxic, immunosuppressive, teratogenic and carcinogenic effects. The aim of the present study was to identify the species of Aspergillus that contaminate the inside of coffee beans collected in the stage of maturation and drying, from 16 producing areas located in the northern region of the State of Paraná, in the South of Brazil. A total of 108 isolates of Aspergillus spp. was identified at the species level, by sequencing the internal transcribed spacer (ITS1-5.8S-ITS2) of ribosomal DNA (rDNA). The results revealed the presence of potentially ochratoxigenic species in 82% of the geographic regions studied, among which Aspergillus niger was the species most frequently detected, followed by A. ochraceus and A. carbonarius. The presence of A. carbonarius in immature coffee fruits harvested from trees is reported for the first time.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000100009
Editor:  São Paulo - Escola Superior de Agricultura "Luiz de Queiroz"
Relação:  10.1590/S0103-90162005000100009
Formato:  text/html
Fonte:  Scientia Agricola v.62 n.1 2005
Direitos:  info:eu-repo/semantics/openAccess
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