| Registro completo |
| Provedor de dados: |
Ciência e Agrotecnologia
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| País: |
Brazil
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| Título: |
Factors affecting the production and regeneration of protoplasts from Colletotrichum lindemuthianum
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| Autores: |
Ishikawa,Francine Hiromi
Barcelos,Quélen de Lima
Souza,Elaine Aparecida de
Dias,Eustáquio Souza
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| Data: |
2010-02-01
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| Ano: |
2010
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| Palavras-chave: |
Protoplast formation
Lytic enzyme
Anthracnose
Osmotic stabilizer
Filamentous fungi
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| Resumo: |
The present work reports factors affecting the production and regeneration of protoplasts from Colletotrichum lindemuthianum. The usefulness of protoplast isolation is relevant for many different applications and has been principally used in procedures involving genetic manipulation. Osmotic stabilizers, lytic enzymes, incubation time and mycelial age were evaluated in terms of their effects on protoplast yield. The optimal condition for protoplast production included the incubation of young mycelia (48 h) in 0.6 mol l-1 NaCl as the osmotic stabilizer, with 30 mg ml-1 Lysing Enzymes from Trichoderma harzianum for 3 h of incubation. In these conditions protoplasts production was higher than 10(6) protoplatos ml-1 in the digestion mixture, number suitable enough for experiments of transformation in fungi. Sucrose concentrations of 1.2 mol l-1 and 1 mol l-1 were the most suitable osmotic stabilizers for the regeneration after 48 h, with rates of 16.35% and 14.54%, respectively. This study produced an efficient method for protoplast production and reverted them into a typical mycelial morphology using a Colletotrichum lindemuthianum LV115 isolate.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000100009
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| Editor: |
Editora da Universidade Federal de Lavras
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| Relação: |
10.1590/S1413-70542010000100009
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| Formato: |
text/html
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| Fonte: |
Ciência e Agrotecnologia v.34 n.1 2010
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| Direitos: |
info:eu-repo/semantics/openAccess
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