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Provedor de dados:  Ciência Rural
País:  Brazil
Título:  Diversity of microsatellites linked to the FSHbeta gene, their usefulness for individual identification and association with reproductive performance
Autores:  Duarte,Letícia Becker Homrich
Moraes,José Carlos Ferrugem
Weimer,Tania de Azevedo
Data:  2005-02-01
Ano:  2005
Palavras-chave:  Microsatellites
Bovine genetic diversity
Individual identification
Reproductive performance
Marker-assisted selection
Resumo:  The genetic diversity of three microsatellites (ILSTS027, MBO22, BM4325) mapped on the bovine chromosome 15 and linked to the follicle-stimulating hormone beta gene (FSHß) was investigated in cows of a Brangus Ibagé herd and the efficiency of these markers for individual identification and parentage control was estimated. Possible associations between each molecular marker and cow reproductive performance were also analyzed. Six alleles were detected in BM4325 and ILSTS027 and 12 in MB022, the most frequent being BM4325*101, BM4325*103, ILSTS027*169 and MB022*229. Polymorphic information content ranged from 0.58 to 0.88 while expected heterozygosity ranged from 65% to 89%, with an average mean value of 77%. Although only three markers were studied, the combined values indicate a high power of exclusion of a false parentage (94%) and of individual identification (3.8 x 10-4). The association analyses based on statistics parameters [MB022 (n=104, CI=545.3±127.0, WFC=349.9±53.4), BM4325 (n=106, CI=542.2±124.9, WFC=350.5±54.4) and ILSTS027 (n =105, CI=543.4 ± 124.5, WFC=350.1±54.5)] indicated no positive association between each microsatellite and weight at first calving. Calving interval (CI) also seemed not to be influenced by the ILSTS027 or MB022 system. However, carriers of at least one BM4325*101 allele presented a CI about 54 days shorter than the other animals (P=0.04; n=106). This marker could be useful for marker-assisted selection, allowing the improvement of reproductive performance, at least in the Brangus Ibagé herd.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782005000100023
Editor:  Universidade Federal de Santa Maria
Relação:  10.1590/S0103-84782005000100023
Formato:  text/html
Fonte:  Ciência Rural v.35 n.1 2005
Direitos:  info:eu-repo/semantics/openAccess
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