| Registro completo |
| Provedor de dados: |
Genet. Mol. Biol.
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| País: |
Brazil
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| Título: |
Karyotypic evolution of ribosomal sites in buffalo subspecies and their crossbreed
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| Autores: |
Degrandi,Tiago Marafiga
Pita,Sebastian
Panzera,Yanina
Oliveira,Edivaldo Herculano C. de
Marques,José Ribamar Felipe
Figueiró,Marivaldo Rodrigues
Marques,Larissa Coêlho
Vinadé,Lucia
Gunski,Ricardo José
Garnero,Analía Del Valle
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| Data: |
2014-06-01
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| Ano: |
2014
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| Palavras-chave: |
18S rDNA
Bubalus bubalis
FISH
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| Resumo: |
Domestic buffaloes are divided into two group based on cytogenetic characteristics and habitats: the "river buffaloes" with 2n = 50 and the "swamp buffaloes", 2n = 48. Nevertheless, their hybrids are viable, fertile and identified by a 2n = 49. In order to have a better characterization of these different cytotypes of buffaloes, and considering that NOR-bearing chromosomes are involved in the rearrangements responsible for the karyotypic differences, we applied silver staining (Ag-NOR) and performed fluorescent in situ hybridization (FISH) experiments using 18S rDNA as probe. Metaphases were obtained through blood lymphocyte culture of 21 individuals, including river, swamp and hybrid cytotypes. Ag-NOR staining revealed active NORs on six chromosome pairs (3p, 4p, 6, 21, 23, 24) in the river buffaloes, whereas the swamp buffaloes presented only five NOR-bearing pairs (4p, 6, 20, 22, 23). The F1 crossbreed had 11 chromosomes with active NORs, indicating expression of both parental chromosomes. FISH analysis confirmed the numerical divergence identified with Ag-NOR. This result is explained by the loss of the NOR located on chromosome 4p in the river buffalo, which is involved in the tandem fusion with chromosome 9 in this subspecies. A comparison with the ancestral cattle karyotype suggests that the NOR found on the 3p of the river buffalo may have originated from a duplication of ribosomal genes, resulting in the formation of new NOR sites in this subspecies.
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| Tipo: |
Info:eu-repo/semantics/article
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| Idioma: |
Inglês
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| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300009
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| Editor: |
Sociedade Brasileira de Genética
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| Relação: |
10.1590/S1415-47572014000300009
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| Formato: |
text/html
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| Fonte: |
Genetics and Molecular Biology v.37 n.2 2014
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| Direitos: |
info:eu-repo/semantics/openAccess
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