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Provedor de dados:  Genet. Mol. Biol.
País:  Brazil
Título:  Chromosomal localization of the 18S-28S and 5S rRNA genes and (TTAGGG)n sequences of butterfly lizards (Leiolepis belliana belliana and Leiolepis boehmei, Agamidae, Squamata)
Autores:  Srikulnath,Kornsorn
Uno,Yoshinobu
Matsubara,Kazumi
Thongpan,Amara
Suputtitada,Saowanee
Apisitwanich,Somsak
Nishida,Chizuko
Matsuda,Yoichi
Data:  2011-01-01
Ano:  2011
Palavras-chave:  Chromosomal mapping
FISH
Interstitial telomeric site
Leiolepidinae
Ribosomal RNA gene
Telomeric sequence
Resumo:  Chromosomal mapping of the butterfly lizards Leiolepis belliana belliana and L. boehmei was done using the 18S-28S and 5S rRNA genes and telomeric (TTAGGG)n sequences. The karyotype of L. b. belliana was 2n = 36, whereas that of L. boehmei was 2n = 34. The 18S-28S rRNA genes were located at the secondary constriction of the long arm of chromosome 1, while the 5S rRNA genes were found in the pericentromeric region of chromosome 6 in both species. Hybridization signals for the (TTAGGG)n sequence were observed at the telomeric ends of all chromosomes, as well as interstitially at the same position as the 18S-28S rRNA genes in L. boehmei. This finding suggests that in L. boehmei telomere-to-telomere fusion probably occurred between chromosome 1 and a microchromosome where the 18S-28S rRNA genes were located or, alternatively, at the secondary constriction of chromosome 1. The absence of telomeric sequence signals in chromosome 1 of L. b. belliana suggested that its chromosomes may have only a few copies of the (TTAGGG)n sequence or that there may have been a gradual loss of the repeat sequences during chromosomal evolution.
Tipo:  Info:eu-repo/semantics/article
Idioma:  Inglês
Identificador:  http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400009
Editor:  Sociedade Brasileira de Genética
Relação:  10.1590/S1415-47572011005000042
Formato:  text/html
Fonte:  Genetics and Molecular Biology v.34 n.4 2011
Direitos:  info:eu-repo/semantics/openAccess
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