| Registro completo |
| Provedor de dados: |
R. Bras. Zootec.
|
| País: |
Brazil
|
| Título: |
Obtaining tetraploid plants of ruzigrass (Brachiaria ruziziensis)
|
| Autores: |
Timbó,Ana Luiza de Oliveira
Souza,Patrícia Nirlane da Costa
Pereira,Roselaine Cristina
Nunes,Juliane Dornellas
Pinto,José Eduardo Brasil Pereira
Souza Sobrinho,Fausto de
Davide,Lisete Chamma
|
| Data: |
2014-03-01
|
| Ano: |
2014
|
| Palavras-chave: |
DNA content
Forage plant breeding
Number of chromosomes
Polyploidy induction
Pollen viability
|
| Resumo: |
The objective of the present work was to induce chromosome doubling in genotypes of Brachiaria ruziziensis with potential for production of good-quality biomass and tolerance/resistance to stress caused by aluminum and spittlebugs. Germinated seeds of B. ruziziensis were immersed in 0.1% colchicine solution for 2 or 3 hours. Ploidy level was determined by flow cytometry and confirmed by chromosome count. Fertility of the tetraploid plants was assessed through evaluation of pollen viability by staining. There was no statistical difference with regard to seedling survival and number of duplicate seedlings between the treatments. Survival rate was 8%, of which 11.45% were tetraploid genotypes. The mean viability of pollen grains from the evaluated tetraploid plants ranged from 51.75 to 55.50%. So, the tetraploids plants obtained from genotypes of a bred population of ruzigrass produce fertile pollen, in percentages that render crosses viable in genetic breeding programs.
|
| Tipo: |
Info:eu-repo/semantics/article
|
| Idioma: |
Inglês
|
| Identificador: |
http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982014000300004
|
| Editor: |
Sociedade Brasileira de Zootecnia
|
| Relação: |
10.1590/S1516-35982014000300004
|
| Formato: |
text/html
|
| Fonte: |
Revista Brasileira de Zootecnia v.43 n.3 2014
|
| Direitos: |
info:eu-repo/semantics/openAccess
|
