Consulta: creatorFacets:"Chen,Xin"
Registros recuperados: 3
Data/hora: 13/06/2024 18:56:48

Effect of nickel chloride on Arabidopsis genomic DNA and methylation of 18S rDNA
Provedor de dados: 69
Autores: Li,Zhongai; Chen,Xin; Li,Suoping; Wang,Zicheng.
Background In recent years, nickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution. In this study, the effect of nickel chloride (NiCl2) with different concentrations on Arabidopsis genomic stability and DNA methylation has been demonstrated. The nucleolus variation and 18S rDNA methylation after NiCl2 treatment have been analyzed. Results The results are as follows: (1) The NiCl2 could result in heritable genomic methylation variations. The genomic DNA methylation variations have been detected by methylation-sensitive amplified polymorphism (MSAP) molecular markers, and the result showed that after NiCl2 treatment, there was methylation variation in T0 generation seedlings, and...
Tipo: Journal article
Palavras-chave: DNA methylation; Heavy metal; Methylation sensitive amplified polymorphism (MSAP); Nickel.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100010

EFFECT OF NITROGEN FERTILIZER AND MAIZE STRAW INCORPORATION ON NH4+15N AND N0(3) -15N ACCUMULATION IN BLACK SOIL OF NORTHEAST CHINA AMONG THREE CONSECUTIVE CROPPING CYCLES
Provedor de dados: 126
Autores: Lu,Caiyan; Ma,Jian; Chen,Xin; Zhang,Xudong; Shi,Yi; Huang,Bin.
A pot experiment was conducted to evaluate the effect of nitrogen (N) fertilizer and maize straw incorporation on the accumulation of NH4+-15N and N0(3)--15N in soil inorganic N pool among three consecutive cropping cycles, aimed to search for an effective N management practice to decrease superfluous accumulation of soil inorganic N and fertilizer N losses. The results showed that the amounts of soil NH4+-15N, N0(3)-15N and inorganic 15N, and their percent to applied 15N-labeled fertilizer declined significantly with sampling time (p &amp;#8804; 0.001).Compared to low N application rate (44.64 mg N kg-1 soil), high N application rate (89.28 mg N kg-1 soil) enhanced significantly the amounts of soil NH4+-15N, N0(3)--15N and inorganic 15N by 238.6%, 132.9%...
Tipo: Journal article
Palavras-chave: N fertilizer; Maize straw; NH4+-15N; N0(3)--15N accumulation; Black soil.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-95162010000200005

Small RNA sequencing reveals microRNAs related to neuropathic pain in rats
Provedor de dados: 56
Autores: Dai,Dawei; Wang,Junyu; Jiang,Ying; Yuan,Lei; Lu,Youming; Zhang,Aijun; Zou,Dongdong; Chen,Xin.
The present study aimed to identify microRNAs (miRNAs) that are involved in neuropathic pain and predict their corresponding roles in the pathogenesis and development process of neuropathic pain. The rat model of neuropathic pain caused by spared nerve injury (SNI) was established in Sprague-Dawley male rats, followed by small RNA sequencing of the L3–L6 dorsal root ganglion. Real-time PCR was performed to validate the differently expressed miRNAs. Functional verification was performed by intrathecally injecting the animals with miRNA agomir. A total of 72 differentially expressed miRNAs were identified in the SNI rats, including 33 upregulated and 39 downregulated miRNAs. The results of qPCR further verified the expression levels of rno-miR-6215...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MicroRNAs; Neuropathic pain; Spared nerve injury; Dorsal root ganglion.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2019001000602