Consulta: creatorFacets:"Fukumoto, Shinya"
Registros recuperados: 4
Data/hora: 04/06/2024 00:14:23

Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs.
Provedor de dados: 19
Autores: Fukumoto, Shinya; Tamaki, Yoh; Okamura, Masashi; Bannai, Hiroshi; Yokoyama, Natsuko; Suzuki, Tomoko; Igarashi, Ikuo; Suzuki, Hiroshi; Xuan, Xuenan.
A heterologous prime-boost immunization regime with priming DNA followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed P50 of Babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibody against P50 when compared with the control groups, and the antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. The prime-boost immunization...
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/812

Dextran sulfate inhibits acute Toxoplama gondii infection in pigs
Provedor de dados: 19
Autores: Kato, Kentaro; Murata, Yuho; Horiuchi, Noriyuki; Inomata, Atsuko; Terkawi, Mohamad Alaa; Ishiwa, Akiko; Ogawa, Yohsuke; Fukumoto, Shinya; Matsuhisa, Fumikazu; Koyama, Kenji.
Background: Toxoplasma gondii is a highly prevalent protozoan that can infect all warm-blooded animals, including humans. Its definitive hosts are Felidae and its intermediate hosts include various other mammals and birds, including pigs. It is found in the meat of livestock which is a major source of human infection. Hence the control of toxoplasmosis in pigs is important for public health. We previously showed that dextran sulfate (DS), especially DS10 (dextran sulfate MW 10 kDa), is effective against T. gondii infection both in vitro and in mice. In this study, we asked whether DS affects T. gondii infection of pigs, one of the main animal sources of toxoplasmosis transmission to humans. Methods: Fourteen-day-old male pigs (n = 10) were infected with T....
Palavras-chave: Dextran sulfate; Intermediate host; Pig; Public health; Toxoplasma gondii.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4446

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: 19
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
Provedor de dados: 19
Autores: Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo.
Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816