Consulta: creatorFacets:"Gitahy,Patrícia de Medeiros"
Registros recuperados: 2
Data/hora: 10/06/2024 16:05:57

A Brazilian Bacillus thuringiensis strain highly active to sugarcane borer Diatraea saccharalis (Lepidoptera: Crambidae)
Provedor de dados: 58
Autores: Gitahy,Patrícia de Medeiros; Souza,Marlene Teixeira de; Monnerat,Rose Gomes; Arrigoni,Enrico de Beni; Baldani,José Ivo.
The control of the major sugarcane pest, Diatraea saccharalis, is limited by the stem location of the caterpillar. As part of a long-term project towards the development of an alternative and efficient delivery system of Cry proteins to control the sugarcane borer, the current work describes the selection and characterization of a Brazilian B. thuringiensis strain with prominent activity towards D. saccharalis. Strain S76 was eleven-fold more active than the HD-1 Lepidoptera-standard strain, as estimated by the LC50 of 13.06 µg/L and 143.88 µg/L, respectively. We observed bipiramidal and cuboidal crystals similar to those found in other B. thuringiensis strains with entomopathogenic activity against Lepidoptera and Diptera. In addition, smaller and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biological control; Bacillus thuringiensis kurstaki; Cry toxins; Spherical crystal; Diatraea saccharalis.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300028

Use of endophytic diazotrophic bacteria as a vector to express the cry3A gene from Bacillus thuringiensis
Provedor de dados: 58
Autores: Salles,Joana Falcão; Gitahy,Patrícia de Medeiros; Skøt,Leif; Baldani,José Ivo.
The goal of this study was to evaluate the potential of endophytic diazotrophic bacteria as a vector to express a cry gene from Bacillus thuringiensis, envisaging the control of pests that attack sugarcane plants. The endophytic nitrogen-fixing bacteria Gluconacetobacter diazotrophicus strain BR11281 and Herbaspirillum seropedicae strain BR11335 were used as models. The cry3A gene was transferred by conjugation using a suicide plasmid and the recombinant strains were selected by their ability to fix nitrogen in semi-solid N-free medium. The presence of the cry gene was detected by Southern-blot using an internal fragment of 1.0 kb as a probe. The production of delta-endotoxin by the recombinant H. seropedicae strain was detected by dot blot while for G....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gluconacetobacter diazotrophicus; Herbaspirillum seropedicae; Endophytic bacteria; Diazotrophic; Cry gene.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300001