Consulta: creatorFacets:"Hirooka,Elisa Yoko"
Registros recuperados: 11
Data/hora: 05/06/2024 10:54:41

Development of ic-Elisa for the screening of aflatoxin contamination in the peanut production chain
Provedor de dados: 86
Autores: Silva,André Ribeiro da; Zanin,Lívia Montanheiro Médici; Ishikawa,Angélica Tieme; Yamashita,Cassia Reika Takabayashi; Fracalossi,Felipe Pedote; Amorim,Thaís Marques; Itano,Eiko Nakagawa; Kawamaura,Osamu; Hirooka,Elisa Yoko.
Abstract: The objective of this work was to standardize and validate an indirect competitive enzyme-linked immunosorbent assay (ic-Elisa), as a low-cost tool, to monitor the presence of aflatoxin in common and blanched peanuts (Arachis hypogaea) in the production chain. The presence of aflatoxin B1, moisture content, and water activity were analyzed in 60 samples of the peanut cultivar Runner IAC 886, from the 2014/2015 and 2015/2016 harvests of the region of Alta Paulista, in the state of São Paulo, Brazil. The validation showed an adequate linearity (R2 = 0.999), and limits of detection and quantification of 1.13 and 3.59 μg kg-1, respectively. Recovery rates of 104, 102, and 107% at the concentrations of 4, 10, and 20 μg kg-1 aflatoxin B1, respectively,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aspergillus; Aflatoxin B1; Blanched peanuts; Contamination; Mycotoxin.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300361

Chemical characterization of wheat kernels naturally contaminated by deoxynivalenol-DON when cultivated under nitrogen management strategies
Provedor de dados: 124
Autores: Souza,Thiago Montagner; Prando,André Mateus; Takabayashi-Yamashita,Cassia Reika; Zucareli,Claudemir; Hirooka,Elisa Yoko.
ABSTRACT Chemical composition and contamination of wheat kernels (Triticum aestivum L.) directly affect the quality of the flour obtained from them, determining its acceptability and use by industry. Field trials were conducted to evaluate the effect of agricultural practices on wheat kernel quality (chemical composition and contamination). Cultivation was carried out following maize or soybean (crop succession), with application of nitrogen doses in topdressing (0, 30, 60, 90, and 120 kg ha-1), using seeds inoculated or not inoculated with Azospirillum brasilense. Data were subjected to analysis of variance (ANOVA), comparison of means by Tukey's test (&lt;0.05), and regression to nitrogen doses. Crop succession resulted in changes in kernel chemical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Triticum aestivum (L.); Crop succession; Nitrogen doses; Azospirillum brasilense; Kernel composition.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902019000400650

Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A
Provedor de dados: 59
Autores: Oliveira,Tereza Cristina R.M. de; Hirooka,Elisa Yoko.
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0, 8, 24, 59, 62 and 67. The amount of toxin at each injection was 5, 6, 20, 50, 100 and 200&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, respectively. The total amount of toxin was 381&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, which corresponded to a reduction of 107&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 1:60 and using ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxins; Detection; Immunization.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200006

Fusarium verticillioides strains isolated from corn feed: characterization by fumonisin production and RAPD fingerprinting
Provedor de dados: 52
Autores: Ono,Elisabete Yurie Sataque; Fungaro,Maria Helena Pelegrinelli; Sofia,Silvia Helena; Miguel,Tatiana de Ávila; Sugiura,Yoshitsugu; Hirooka,Elisa Yoko.
In this study a total of 16 Fusarium verticillioides strains isolated from corn feed samples were characterized by fumonisin (FB) production and random amplified polymorphic DNA (RAPD). All the strains produced FB1 and FB2 with levels ranging from 2.41 to 3996.36 µg/g, and from 1.18 to 1209.91 µg/g, respectively. From the 16 F. verticillioides strains, four were identified as low (3.59 to 1289.84 µg/g), eight as intermediate (&gt;1289.84 to 3772.44 µg/g) and four strains as high (&gt;3772.44 µg/g) fumonisin producers. From the total of 105 loci amplified, 60 (57.14%) were polymorphic. RAPD analysis showed very similar patterns among low, moderate and high fumonisin-producing strains. Although RAPD markers were capable of discriminating the different F....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fumonisin; Fusarium verticillioides; Genetic variability; RAPD.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400026

Penicillium expansum versus antagonist yeasts and patulin degradation in vitro
Provedor de dados: 52
Autores: Coelho,Alexandre Rodrigo; Celli,Marcos Giovani; Ono,Elisabete Yurie Sataque; Wosiacki,Gilvan; Hoffmann,Fernando Leite; Pagnocca,Fernando Carlos; Hirooka,Elisa Yoko.
Taking into account the preliminary antagonistic/biodegradation property showed by Pichia membranifaciens and Sporobolomyces roseus, which decreased the initial patulin concentration of 588.4 to 290.0 µg/mL, ability of P. ohmeri 158 in biocontrol against Penicillium expansum and patulin decrease in vitro was performed. The culture supernatant of P. ohmeri 158 was effective against 66.17% micelial growth, indicating antibiosis related with the killer phenomenon. The initial patulin concentration of 223 µg in the presence of P. ohmeri 158 cells was decreased over 83% of the original concentration, when incubated at 25ºC/2 days and &gt; 99% after 5 days incubation time, with undetectable patulin level after 15 days. The initial pH 4.0 decreased to pH 3.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Penicillium expansum; Antagonist yeasts; Biocontrol; Patulin; Degradation.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000400019

A comparison between enzyme immunoassay and HPLC for ochratoxin A detection in green, roasted and instant coffee
Provedor de dados: 52
Autores: Fujii,Simone; Ono,Elisabete Yurie Sataque; Ribeiro,Ricardo Marcelo Reche; Assunção,Fernanda Garcia Algarte; Takabayashi,Cássia Reika; Oliveira,Tereza Cristina Rocha Moreira de; Itano,Eiko Nakagawa; Ueno,Yoshio; Kawamura,Osamu; Hirooka,Elisa Yoko.
An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) for ochratoxin A (OTA) detection in green, roasted and instant coffees was developed using anti-OTA monoclonal antibody. Immunological reagents prepared were OTA-BSA (4.76 mg/mL), anti-OTA.7 MAb (2x10³-fold dilution) and HRP-anti IgG (10³-fold dilution). The detection limit was 3.73 ng OTA/g and correlation coefficients (r) between this immunoassay and high performance liquid chromatography were 0.98 for green coffee, 0.98 for roasted and 0.86 for instant. OTA levels detected by ic-ELISA were higher than by HPLC, with ELISA/HPLC ratio of 0.66 - 1.46 (green coffee), 0.96 - 1.11 (roasted) and 0.93 - 1.82 (instant). ELISA recoveries for OTA added to coffee (5 - 70 ng/g) were 81.53 % for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ochratoxin; Immunoassay; HPLC; Monoclonal antibody; Coffee.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132007000200020

Comparison of thin-layer chromatography, spectrofluorimetry and bright greenish-yellow fluorescence test for aflatoxin detection in corn
Provedor de dados: 52
Autores: Ono,Elisabete Yurie Sataque; Silva,Marcelo da; Ribeiro,Ricardo Marcelo Reche; Ono,Mario Augusto; Hayashi,Luciana; Garcia,Glauco Tironi; Hirooka,Elisa Yoko.
In this study the bright greenish-yellow fluorescence test, widely used by the corn milling industry, was compared to the thin-layer chromatography (TLC) and spectrofluorimetry methods for aflatoxin detection in 40 corn samples naturally contaminated by the Aspergillus section Flavi. According to the corn processing industry criteria, all the samples were adequate for human and animal consumption by the bright greenish-yellow fluorescence test, but TLC and spectrofluorimetry analysis detected aflatoxins above the maximum tolerated limit (20 µg/kg) in 7 and 8 samples, respectively. Aflatoxins were detected in 16 (40%) corn samples by TLC, with levels ranging from 4.0 to 54.0 µg/kg (mean 19.97 ± 15.97 µg/kg), and in 25 (62.5%) samples by spectrofluorimetry,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Thin-Layer Chromatography; Spectrofluorimetry; BGYF; Aflatoxins; Corn; Mycotoxins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000300025

Biomonitoring of microcystin and aflatoxin co-occurrence in aquaculture using immunohistochemistry and genotoxicity assays
Provedor de dados: 52
Autores: Hashimoto,Elisabete Hiromi; Kamogae,Márcia; Vanzella,Tatiana Perez; Cólus,Ilce Mara Syllus; Bracarense,Ana Paula Frederico Rodrigues Lourenço; Bittencourt-Oliveira,Maria do Carmo; Itano,Eiko; Kuroda,Emília Kiyomi; Kato,Hajime; Nagata,Satoshi; Ueno,Yoshio; Harada,Ken-Ichi; Hirooka,Elisa Yoko.
This work investigated the effects of co-occurring aflatoxin B1 (AFB1) and microcystin (MC) in aquaculture, using immunohistochemistry and genotoxicity methods. Tilapia (Oreochromis niloticus) were exposed to AFB1 by intraperitoneal and MC (cell extract of Microcystis aeruginosa) by intraperitoneal and immersion routes. The interaction of MC-AFB1 was evaluated co-exposing the intraperitoneal doses. Blood samples were collected after 8, 24, and 48h to analyze the micronucleus frequency and comet score. The interaction of MC-AFB1 showed a synergic mutagenic response by higher micronucleus frequency of co-exposed group. A slight genotoxic synergism was also observed in the comet score. Immunohistochemistry detected MC in al lthe fish liver tissues exposed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Microcystis aeruginosa; Mycotoxin and tilapia.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100019

Culture medium for amylase production by toxigenic fungi
Provedor de dados: 52
Autores: Figueira,Edson Luiz Zangrando; Hirooka,Elisa Yoko.
Mycelial growth and amylase production by a mycotoxigenic strain of Fusarium moniliforme and Aspergillus flavus were evaluated in a culture medium containing starch, glycerol, wheat bran or corn. With emphasis on corn, different fractions composed by germ, degermed seed, starch, milky stage corn and the respective starch or supernatant fraction were analyzed for F. moniliforme growth . The medium composed of milky stage corn supernatant promoted the best mycelial growth (p&lt;0.05), and it was used to prepare amylase production medium in the next step. The medium composed with 2% ground corn in milky stage corn supernatant (350g of milky stage corn blended with 250mL water and centrifuged) promoted the highest amylase production, which was at the 10th day...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amylase; Fusarium moniliforme; Aspergillus flavus.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000500003

Fumonisins in corn: correlation with Fusarium sp. count, damaged kernels, protein and lipid content
Provedor de dados: 52
Autores: Ono,Elisabete Yurie Sataque; Biazon,Luciana; Silva,Marcelo da; Vizoni,Édio; Sugiura,Yoshitsugu; Ueno,Yoshio; Hirooka,Elisa Yoko.
Natural fungal and fumonisin contamination were evaluated in 109 freshly harvested corn samples from Paraná State and correlated to damaged kernels (%). In addition, healthy and damaged kernels of 24 corn samples were selected in order to compare the mycoflora profile and fumonisin levels. The correlation among protein/lipid content and fumonisin levels was also analyzed in the 15 most frequently cultivated corn hybrids. Total fungal colony count in 109 freshly harvested corn samples ranged from 1.9x10(4) to 3.5x10(6) CFU/g, Fusarium sp. count from 1.0x10³ to 2.2x10(6) CFU/g, and fumonisin levels from 0.13 to 20.38 µg/g. Total fungal colony/Fusarium sp. count and fumonisin levels showed positive correlation (p &lt; 0.05). In addition, there was a positive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoflora; Toxigenic fungi; Mycotoxins; Fumonisins; Corn quality.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132006000100008

Blackberry (Rubus spp.): influence of ripening and processing on levels of phenolic compounds and antioxidant activity of the 'Brazos' and 'Tupy' varieties grown in Brazil
Provedor de dados: 65
Autores: Zielinski,Acácio Antonio Ferreira; Goltz,Caroline; Yamato,Maira Akemi Casagrande; Ávila,Suelen; Hirooka,Elisa Yoko; Wosiacki,Gilvan; Nogueira,Alessandro; Demiate,Ivo Mottin.
Fruits from temperate and tropical climates which have high levels of antioxidant compounds are the source of numerous studies concerning the correlation with benefits to human health. The objectives of this study were to quantify the anthocyanins and phenolic compounds and also to measure the antioxidant activity (ferric reducing antioxidant power - FRAP) of blackberries from two varieties grown in southern Brazil ('Brazos' and 'Tupy') at three stages of ripening; unripe, semi-ripe, ripe and their products (pulp and fermented products). During fruit ripening it was observed that weight, size, diameter and sugars increase significantly and acidity decreased significantly. The anthocyanin content ranged from 4.19 (semi-ripe 'Tupy' variety) to 205.75mg...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rubus spp.; Antioxidant activity; Ripening stage; Processing.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000400744