Consulta: creatorFacets:"INOKUMA, Hisashi"
Registros recuperados: 77
Data/hora: 03/06/2024 18:53:29

Serological survey of Borrelia infection of dogs in Sapporo, Japan, where Borrelia garinii infection was previously detected
Provedor de dados: 19
Autores: UESAKA, Karin; MAEZAWA, Masaki; INOKUMA, Hisashi; 猪熊, 壽.
A serological survey of Borrelia infection of dogs was performed in Sapporo, Japan, where Borrelia garinii infection in dogs was detected in 2011. A total of 314 serum samples were collected from dogs that visited three animal hospitals in Sapporo from 2012 to 2014. The two-step evaluation method, involving screening ELISA followed by Western blot analysis, was used to detect antibodies against Borrelia species. A total of 34 samples were positive by ELISA. Among those 34 samples, 32 were positive for Borrelia spp. by Western blot. These findings suggest that the 32 dogs (10.2%) generated antibodies against Borrelia burgdorferi sensu lato, such as B. garinii or B. afzelii. Antibody positivity was 7.6% and 13.3% for dogs living in urban and rural areas,...
Palavras-chave: Borrelia burgdorferi sensu lato; Canine; Sapporo; Serosurvey.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4277

Molecular Survey of Babesia gibsoni Using Haemaphysalis longicornis Collected from Dogs and Cats in Japan
Provedor de dados: 19
Autores: IWAKAMI, Shinya; ICHIKAWA, Yasuaki; INOKUMA, Hisashi; 猪熊, 壽.
A nationwide survey of Babesia gibsoni using Haemaphysalis longicornis collected from dogs and cats in Japan was conducted using molecular methods. A total of 1,341 H. longicornis, including 305 females, 14 males, 332 nymphs and 690 larvae (153 pools) from 44 prefectures, were examined by B. gibsoni-targeted PCR. Partial sequence analysis revealed that 12 of 13 positive samples sequenced, including samples from Tottori, Hiroshima, Yamaguchi, Tokushima, Ehime and Oita prefectures (all in western Japan), were identical to B. gibsoni, and 1 sample from Kyoto Prefecture was most closely related to a Babesia species recently detected from feral raccoons in Hokkaido. H. longicornis is a candidate for transmission vector tick of the new Babesia species.
Palavras-chave: Babesia gibsoni; Distribution Haemaphysalis longicornis.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4013

ワンポイント質問 牛のてんかんについて
Provedor de dados: 19
Autores: 猪熊, 壽; INOKUMA, Hisashi.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4388

腹腔内に形成され腫瘍組織の脊柱管内直接浸潤により後躯麻痺を呈した牛白血病の1 例
Provedor de dados: 19
Autores: 松山, 雄喜; 神尾, 恭平; 村上, 智亮; 下田, 崇; 古林, 与志安; 古岡, 秀文; 松本, 高太郎; 猪熊, 壽; MATSUYAMA, Yuuki; KAMIO, Kyouhei; MURAKAMI, Tomoaki; SHIMODA, Takashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUMOTO, Kohtaro; INOKUMA, Hisashi.
5 歳6 カ月齢ホルスタイン種乳牛が，後躯麻痺による起立不能を呈した．浅頸リンパ節の腫大と直腸検査による腫瘤 の触知から牛白血病を疑ったが，血液検査とリンパ節の針吸引生検では確定診断できなかった．剖検では，60 × 40 × 20cm 大の多結節性腫瘤が背側の腹腔壁に接して存在していた．腫瘍組織は腸腰筋に浸潤し，一部は第5 および第6 腰 椎椎間孔より脊柱管内に連続的に波及し，脊髄および脊髄神経根を圧迫していた．腫瘍はCD3 陽性のT細胞性リンパ 腫で，牛白血病ウイルス抗体陰性であったため，散発型牛白血病と診断された． A Holstein cow (5 years and 3 months old) presented with hind limb paresis and was unable to stand. Bovine leukosis was suspected given the enlargement of cervical lymph nodes and existence of an abdominal mass detected by rectal palpation; however，it could not be confirmed by blood examination and fine needle aspiration of cervical lymph nodes. Pathological examination revealed a mu1tinodular mass (60 x 40 x 20 cm) on the dorsal abdomen that was invasive into the iliopsoas muscle. A portion of the...
Palavras-chave: 牛白血病; 脊髄浸潤; 後躯麻痺; Bovine leukosis; Spinal cord invasion; Hind limb paresis.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2809

Detection of the nonsense mutation of OPA3 gene in Holstein Friesian cattle with dilated cardiomyopathy in Japan
Provedor de dados: 19
Autores: HORIUCHI, Noriyuki; KUMAGAI, Daishiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安.
Bovine dilated cardiomyopathy (DCM) is an autosomal recessive genetic disorder causing congestive heart failure and subsequent death. Recently, a nonsense mutation c.343C&gt;T in the bovine optic atrophy 3 (OPA3) gene had been reported to cause the DCM in Holstein cattle in Switzerland. However, the mutation has not been confirmed in bovine DCM outside Switzerland. Nine Holstein Friesian cows that were macroscopically and histologically diagnosed with or suspected of DCM and 12 control cows kept in Japan were tested for the mutation. The mutation surrounding OPA3 DNA fragment was amplified by PCR and subjected to direct sequences. The homogeneous c.343C&gt;T mutation was proved to occur in all the affected cows and not in the control cows. The present...
Palavras-chave: Cattle; Dilated cardiomyopathy; Nonsense mutation; OPA3 gene.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4208

A Mechanism of Excessive Accumulation of Abomasal Gas in Vagotomized Cattle Determined using Fluoroscopy
Provedor de dados: 19
Autores: ITOH, Megumi; SASAKI, Naoki; KAWAMOTO, Satoshi; YAMADA, Haruo; INOKUMA, Hisashi; 佐々木, 直樹; 猪熊, 壽.
To better understand the mechanism of excessive gas accumulation in the abomasum in bovine abomasal displacement, we performed gastric fluoroscopy in vagotomized cattle. Fifteen 6-month-old Holstein steers were divided into three groups: a non-vagotomized control group (Group C; n=5), a ventral thoraco-vagotomized group (Group V; n=5), and a dorsal and ventral thoraco-vagotomized group (Group DV; n=5). These groups were examined by fluoroscopy before and during a 5-week observation period after surgery. In Group C, no change was observed throughout the observation period. In Group DV, immediately after surgery, reticuloruminal motility was completely absent and ruminal distention was seen. Two weeks after surgery, abnormal reticulum motility and increased...
Palavras-chave: Abomasum gas; Cattle; Displaced abomasum; Vagotomy.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3888

Survey on Tick-Borne Pathogens in Thoroughbred Horses in the Hidaka District, Hokkaido, Japan
Provedor de dados: 19
Autores: YBAÑEZ, Adrian Patalinghug; SATO, Fumio; NAMBO, Yasuo; FUKUI, Takashi; MASUZAWA, Toshiyuki; OHASHI, Norio; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; INOKUMA, Hisashi; 松本, 高太郎; 猪熊, 壽.
A total of 87 Thoroughbred horses and 10 ixodid ticks from a ranch in Hidaka district, Hokkaido were tested for tick-borne diseases. Using the indirect fluorescent antibody (IFA) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥80 against Anaplasma phagocytophilum, Rickettsia helvetica, and Borrelia garinii, respectively. This is the first report of infection with the 3 pathogens in horses in Japan. Using PCR, DNAs from the peripheral blood of all horses were found negative with any Anaplasma, Rickettsia and Borrelia spp., while those from Haemaphysalis megaspinosa ticks were found positive for Anaplasma sp. closely related to A. phagocytophilum in Japan, and A. bovis. B. japonica was also detected in an H. flava tick for the first time.
Palavras-chave: Anaplasma sp.; Borrelia sp.; Rickettsia sp.; Thoroughbred horse; Tick-borne diseases.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3840

Use of Contrast-Enhanced CT in the Diagnosis of Abscesses in Cattle
Provedor de dados: 19
Autores: LEE, Ki-Ja; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi; IWASAKI, Toshiroh; MIYAKE, Yoh-Ichi; YAMADA, Kazutaka; 古林, 与志安; 松本, 高太郎; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; 山田, 一孝.
We report here the non-contrast and contrast-enhanced computed tomography performed in two calves with brain abscess and multiple pulmonary abscesses with pharyngeal abscess, respectively. Contrast-enhanced computed tomography was useful in the diagnosis of these diseases in both calves. The diseases were confirmed by histopathological examination.
Palavras-chave: Abscess; Cattle; Contrast-enhanced CT; Diagnosis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3891

A Clinical Case of Severe Anemia in a Sheep Coinfected with Mycoplasma ovis and 'Candidatus Mycoplasma haemovis' in Hokkaido, Japan
Provedor de dados: 19
Autores: TAGAWA, Michihito; TAKEUCHI, Toshihiko; FUJISAWA, Tetsuro; KONNO, Yasuhiro; YAMAMOTO, Shinji; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
A 2-year-old East Friesian sheep imported from Australia exhibited severe anemia after contagious pustular dermatitis in Hokkaido, Japan. Hemoplasma infection was confirmed in blood smears. Both Mycoplasma ovis and ‘Candidatus Mycoplasma haemovis’ were detected by PCR and sequence analyses. In the epidemiological analysis, dual pathogens were detected in 6 of 12 (50.0%) sheep imported from Australia with the infected ewe at the same time, 1 of 5 (20.0%) sheep introduced from a domestic farm in Hokkaido, and in 1 of 16 (6.3%) sheep from an epidemiologically unrelated ranch. It is the first clinical case of sheep to confirm coinfection of these pathogens in Japan.
Palavras-chave: 'Candidatus Mycoplasma haemovis'; Case report; Japan; Mycoplasma ovis; Sheep.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3582

急性鼻出血を主訴とするホルスタイン種乳牛10症例における臨床所見の病態別比較
Provedor de dados: 19
Autores: 猪熊, 壽; 滄木, 孝弘; 松本, 高太郎; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; MATSUMOTO, Kotaro; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4145

Detection of monoclonal integration of bovine leukemia virus proviral DNA as a malignant marker in two enzootic bovine leukosis cases with difficult clinical diagnosis
Provedor de dados: 19
Autores: MIURA, Saori; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; KAWAZU, Shin-ichiro; INOKUMA, Hisashi.
Monoclonal integration of bovine leukemia virus (BLV) proviral DNA into bovine genomes was detected in peripheral blood from two clinical cases of enzootic bovine leukosis (EBL) without enlargement of superficial lymph nodes. A BLV-specific probe hybridized with 1 to 3 EcoRI and HindIII fragments in these 2 atypical EBL cattle by Southern blotting and hybridization, as well as in 3 typical EBL cattle. The probe also hybridized to a large number of EcoRI and HindIII fragments in 5 cattle with persistent leukosis. These results suggest that the detection of monoclonal integration of BLV provirus into the host genome may serve as a marker of monoclonal proliferation and malignancy in difficult to diagnose EBL cattle.
Palavras-chave: Atypical enzootic bovine leukosis; Diagnostic marker; Monoclonal integration.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4143

心内膜炎の併発がみられた心室中隔欠損のホルスタイン種成乳牛の1症例
Provedor de dados: 19
Autores: 岩上, 慎哉; 新谷, 紗代; 髙橋, 英二; 松本, 高太郎; 古岡, 秀文; 猪熊, 壽; MATSUMOTO, Kotaro; FURUOKA, Hidefumi; INOKUMA, Hisashi.
心室中隔欠損とは胎生期に中隔の形成過程が障害され る先天性心奇形である［１、５－７］。牛では最も多く見られる 心奇形で、臨床症状は欠損孔の大きさと位置により様々 であり、一般的には発育不良やチアノーゼを呈するが、 軽度なものでは無症状のまま成長する場合もある［１、５－７］。 一方、心内膜炎は弁膜表面に細菌が付着して炎症性変化 が起こる疾患であり、牛では、ほとんどが疣贅性で、特 に三尖弁と肺動脈弁に生じやすいとされている［２－４、６、７］。 今回、肺動脈弁と三尖弁に心内膜炎を併発した心室中隔 欠損のホルスタイン種成乳牛の１症例に遭遇したので、 その概要を報告する。
Palavras-chave: 心内膜炎; 心室中隔欠損; ホルスタイン.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4008

Comparison of Bacterial Endotoxin Lipopolysaccharide Concentrations in the Blood, Ovarian Follicular Fluid and Uterine Fluid: A Clinical Case of Bovine Metritis
Provedor de dados: 19
Autores: MAGATA, Fumie; ISHIDA, Yoshikazu; MIYAMOTO, Akio; FURUOKA, Hidefumi; INOKUMA, Hisashi; SHIMIZU, Takashi; 宮本, 明夫; 古岡, 秀文; 猪熊, 壽; 清水, 隆.
We investigated the concentration of the bacterial endotoxin lipopolysaccharide (LPS) in the blood, ovarian follicular fluid and uterine fluid of a clinical case of bovine metritis. A 2-year-old lactating Holstein cow exhibited continuous fever &gt;39.5°C for more than 2 weeks after normal calving. The cow produced a fetid, watery, red-brown uterine discharge from the vagina and was diagnosed with metritis. The LPS concentrations in plasma and uterine fluid were 0.94 and 6.34 endotoxin units (EU)/ml, respectively. One of seven follicles showed an extremely high level of LPS (12.40 EU/ml) compared to the other follicles (0.62–0.97 EU/ml). These results might suggest the presence of high concentration of LPS in follicles in cows with postpartum metritis.
Palavras-chave: Dairy cow; Follicle; Lipopolysaccharide; Metritis.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4043

血清チミジンキナーゼ活性の測定により早期摘発した地方病性牛白血病罹患牛の1例
Provedor de dados: 19
Autores: 坂本, 礼央; 大林, 哲; 古林, 与志安; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽; SAKAMOTO, Leo; OHBAYASHI, Tetsu; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi.
チミジンキナーゼはDNA合成に関わる酵素の一つであり，近年牛白血病の発症マーカーとして利用できることが報 告されている.今回，牛白血病ウイルス汚染農場において，本酵素の活性を測定することにより，牛白血病罹患牛の早期摘発を試みた.その結果，臨床症状は示さなかったものの，地方病性牛白血病に罹患していた10歳9カ月齢のホルスタイン種雌牛を摘発できた.牛白血病ウイルス汚染牛群における本酵素活性の測定が，地方病性牛白血病罹患牛の早期摘発に臨床上有用であることが本研究により示唆された. Thymidine kinase is an enzyme related to DNA　synthesis. Recently，serum thymidine kinase (STK) activity has been reported as a useful marker of bovine leukosis. In an effort to achieve earlier detection of enzootic bovine leukosis (EBL)，we measured STK activity in cows at a farm tainted by bovine leukemia virus (BLV). We found that a Holstein cow aged 10 years and 9 months without any clinical symptoms showed higher STK activity，and the cow was diagnosed as having EBL. Evaluation of STK activity in a herd contaminated...
Palavras-chave: 地方病性牛白血病; 早期摘発; 血清チミジンキナーゼ活性; Early case detection; Enzootic bovine leukosis; Serum thymidine kinase activity.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2942

Detection of the New Ehrlichia Species Closely Related to Ehrlichia ewingii from Haemaphysalis longicornis in Yonaguni Island, Okinawa, Japan
Provedor de dados: 19
Autores: MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi.
We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity...
Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581

Thymic Lymphosarcoma with Brain Involvement in a Holstein Heifer
Provedor de dados: 19
Autores: TAWFEEQ, Mohammad Monir; MIURA, Saori; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽.
An 8-month-old Holstein heifer with cervical enlargement was suspected of thymic lymphosarcoma given clinical signs of depression, tendency to lie down, cervical mass, jugular vein distension, conjunctival hyperemia, and ruminal tympany. Unilateral Horner’s syndrome was also observed. Increased serum total lactate dehydrogenase (LDH), LDH isozyme (LDH-2, and LDH-3) and serum thymidine kinase activity were observed. The findings of fine needle aspiration cytology of the cervical mass revealed large lymphoblasts with mitoses present. These findings strongly suggested the diagnosis of lymphosarcoma. Necropsy revealed a large mass in the cervical thymic region, which compressed the esophagus and trachea. Cranial masses in the frontal sinus and multiple...
Palavras-chave: Brain involvement; Cattle; Thymic lymphosarcoma.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4098

Congestive heart failure caused by an arteriovenous fistula between the brachiocephalic trunk and the vena cava cranialis in a Holstein cow
Provedor de dados: 19
Autores: 嘉陽, 静香; 二宮, 理紗; 古関, 博; 堀内, 雅之; 古林, 与志安; 猪熊, 壽; Horiuchi, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
2歳6カ月齢のホルスタイン種成乳牛が水様性下痢を主訴に受診した．第8病日には，胸垂の冷性浮腫および頸静脈怒張が発現し，収縮期駆出性雑音が聴取された．血液検査，心電図検査および心臓超音波検査を実施したが，生前にうっ血性心不全の原因は特定できなかった．病理解剖により，腕頭動脈と前大静脈の短絡が確認された．また，前縦隔において前大静脈に大型の静脈瘤（直径10cm）が形成されていた．本症例は，先天性動静脈瘻の結果，静脈瘤形成とうっ血性心不全を呈した成牛の稀な症例と思われた．
Palavras-chave: 先天性動静脈瘻; うっ血性心不全; 駆出性雑; 静脈瘤.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4146

膣内腫瘤の細胞診が臨床診断に有用であった黒毛和種繁殖牛の地方病性牛白血病の1症例
Provedor de dados: 19
Autores: 前澤, 誠希; 大戸, 千帆; 関塚, 次郎; 堀内, 雅之; 古林, 与志安; 猪熊, 壽; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
Palavras-chave: 膣内腫瘤; 細胞診; 地方病性牛白血病.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4259

Astasia and pyrexia related to Borrelia garinii infection in two dogs in Hokkaido, Japan.
Provedor de dados: 19
Autores: INOKUMA, Hisashi; MAETANI, Shigeki; FUJITSUKA, Jyunji; TAKANO, Ai; SATO, Kozue; FUKUI, Takashi; MASUZAWA, Toshiyuki; KAWABATA, Hiroki; 猪熊, 壽.
Two dogs that exhibited sudden astasia, anorexia and fever higher than 40°C were suspected of having Lyme disease in July 2011. Clinical symptoms gradually improved with antibiotic treatment in both cases. Polymerase chain reaction and sequence analysis revealed Borrelia garinii DNA fragments in the peripheral blood in the acute disease phase. Serological tests, including enzyme linked immunosorbent assay and Western blot analysis, showed an increased IgG antibody titer against Borrelia pathogens in one of the dogs. These findings suggested that diagnosis of the two dogs was Lyme disease related to B. garinii infection.
Palavras-chave: Borrelia garinii; Canine; Japan; Lyme disease.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4099

Optic pathway degeneration in Japanese black cattle
Provedor de dados: 19
Autores: CHIBA, Shiori; FUNATO, Shingo; HORIUCHI, Noriyuki; MATSUMOTO, Kotaro; INOKUMA, Hisashi; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古岡, 秀文; 古林, 与志安.
Degeneration of the optic pathway has been reported in various animal species including cattle. We experienced a case of bilateral optic tract degeneration characterized by severe gliosis in a Japanese black cattle without any obvious visual defects. To evaluate the significance, pathological nature and pathogenesis of the lesions, we examined the optic pathway in 60 cattle (41 Japanese black, 13 Holstein and 6 crossbreed) with or without ocular abnormalities. None of these animals had optic canal stenosis. Degenerative changes with severe gliosis in the optic pathway, which includes the optic nerve, optic chiasm and optic tract, were only observed in 8 Japanese black cattle with or without ocular abnormalities. Furthermore, strong immunoreactivity of...
Palavras-chave: Axonal degeneration; Gliosis; Japanese black cattle; Optic pathway degeneration.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4123

Retrospective study of eleven Holstein dairy cows in which bovine leukosis was suspected but ruled out
Provedor de dados: 19
Autores: 猪熊, 壽; 堀内, 雅之; 古林, 与志安; INOKUMA, Hisashi; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu.
臨床的に牛白血病を疑ったものの，牛白血病ではないと確定診断されたホルスタイン種乳牛11症例の臨床所見を回顧的に整理した．11症例は体表リンパ節腫大あるいは体腔内の腫瘤触知により牛白血病との鑑別診断が必要であったが，病理解剖により乳房炎，子宮蓄膿症，膿瘍，肺炎，腸炎またはアミロイド症と診断された．牛白血病ウイルス（Bovine leukemia virus: BLV）抗体陽性を呈した4頭のうち，リンパ球増多症を呈したものが2頭であった．またこれら2頭のうち1頭はリンパ節細胞診でも大型リンパ球優勢であった．しかし，血清チミジンキナーゼ（Tymidine kinase: TK），総乳酸脱水素酵素（Lactate dehydrogenase: LDH），LDHアイソザイム2および3の活性値は4頭とも基準値以内であり，牛白血病発症は否定的であった．BLV抗体陰性牛7頭のうち，リンパ節細胞診を実施した2頭は小型リンパ球優勢であり，また，リンパ球増多症を呈した症例はいなかった．1症例では血清TK活性値が基準値をわずかに超えており，また，他の2頭は総LDH，LDHアイソザイム2および3の活性値がいずれも基準値を超えていたが，他の検査項目は牛白血病について否定的であった．腫大リンパ節の細胞診，リンパ球数算定，血清TK，総LDH，LDHアイソザイム2および3の活性値測定といった牛白血病診断のための検査を複数実施することで，牛白血病を否定するための診断精度を高めることが可能と考えられた．
Palavras-chave: 牛白血病; 鑑別診断; 血清チミジンキナーゼ; LDH; LDHアイソザイム.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4367

種特異的ELISAによる日本の犬におけるNeospora caninumの血清疫学調査(寄生虫病学)
Provedor de dados: 19
Autores: 久保田, 直樹; 坂田, 義美; 宮崎, 直美; 板本, 和仁; 坂内, 天; 西川, 義文; 玄, 学南; 猪熊, 壽; NISHIKAWA, Yoshifumi; XUAN, Xuenan; INOKUMA, Hisashi.
組換抗原を用いた種特異的ELISAにより,犬のNeospora caninum感染について全国的血清疫学調査を実施した.1,206頭中全国30県から126頭(10.4%)が陽性を示した.陽性率は,雄よりも雌で有意に高く,また他品種と比較して有意に高い陽性率を示す品種がみられた.子宮蓄膿症および糖尿病の病歴を有する犬の陽性率は,他の疾病を有する犬または疾病のない犬の陽性率に比べて高値であった.  A seroepidemiological survey of Neospora caninum infection among dogs in Japan was conducted using species-specific enzyme-linked immunosorbent assay with recombinant surface antigen (Nc-SAG1t). Among 1,206 dogs examined, 126 dogs (10.4%) from 30 prefectures from Hokkaido to Okinawa were positive to N. caninum infections, which were more frequently detected in females than males. Siberian Huskies showed the highest positive rate compared with the other breeds. Dogs with pyometra and diabetes mellitus showed the higher positive rates than dogs with other...
Palavras-chave: Canine; Nbospora caninum; Species-specific ELISA.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4003

特異的抗体検出キットを利用した犬のEhrlichia canisおよびAnaplasma ρhagocytoρhilum感染に関する全国的血清疫学調査
Provedor de dados: 19
Autores: 坂田, 義美; 市川, 康明; 猪熊, 壽; SAKATA, Yoshimi; ICHIKAWA, Yasuaki; INOKUMA, Hisashi.
犬のE.canisおよびA.ρhagocytophilum感染状況を明らかにするため，従来法である蛍光抗体法(IFA)，および組換え主要膜表面蛋白質を抗原とした酵素抗体法を原理とする新規の特異抗体検出キットを組合せて全国的血清疫学調査を行った. IFAによるスクリーニング検査では1,206検体中64頭(5.3%)がE.canis 20倍以上の抗体価を示したが，これら64頭は特異抗体検出キットではすべてE.canis陰性であり，E. canis以外の近縁種に感染していたと考えられた.いっぽうIFAでA.ρhagocytophilumに対して20倍以上の抗体価を示したものは1,206検体中3頭(0.2%)であった.うち1頭は特異抗体検出キットでもA.phagocytophilum陽性を示し，日本にもA.phagocytophilumに感染した犬 がいることが示唆された.しかし，抗体陽性犬に臨床症状は認められず，病原性との関連は不明であった. A seroepidemiological survey of Ehrlichia canis and Anaplasma phagocytophilum infection in dogs in Japan was performed by using a combination of a classical immunofluorescence antibody assay (IFA) and a new enzyme immune assay (EIA) kit that uses a specific recombinant major surface protein of the pathogens. Although 64 (5.3%) of 1,206...
Palavras-chave: 犬; エールリッヒア; アナプラズマ; 血清疫学調査; Anaplasma phagocytophilum; Dog; Ehrlichia canis.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2818

Dual Presence of Anaplasma phagocytophilum and Its Closely Related Anaplasma sp. in Ixodid Ticks in Hokkaido, Japan, and Their Specific Molecular Detection
Provedor de dados: 19
Autores: YBAÑEZ, Adrian Patalinghug; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma phagocytophilum causes human granulocytic anaplasmosis (HGA) and tick-borne fever in ruminants. A closely related and potentially novel Anaplasma sp. in Japan was recently characterized. The aims of the study were to provide molecular evidence for the presence of these 2 species in Japan, and to develop a reliable PCR method based on the nucleotide differences within the citrate synthase (gltA) gene. DNA samples from 182 ixodid ticks (134 Ixodes persulcatus, 35 Haemaphysalis douglasii and 13 I. ovatus) collected from 2 sites in Hokkaido, Japan, were screened for A. phagocytophilum and its closely related Anaplasma sp. (herein designated as Anaplasma sp. Japan) using 16S rRNA PCR, revealing a combined prevalence rate of 27.5% (50 samples). The...
Palavras-chave: 16S rRNA; Anaplasma phagocytophilum; Anaplasma sp.; GltA; GroEL.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3839

ニホンジカに寄生するタイレリアにみられたp33/34遺伝子の変異(寄生虫病学)
Provedor de dados: 19
Autores: 猪熊, 壽; 永田, 美和; 細井, 栄嗣; 板本, 和仁; 奥田, 優; INOKUMA, Hisashi.
全国8地域のニホンジカに寄生するタイレリアの18S rRNAおよびp33/34遺伝子を解析した. 18S rRNA遺伝子の相同性は99.7-100%であったが,山口県産ニホンジカのタイレリアp33/34遺伝子の相同性は,他地域のものに比べて塩基配列で68.7〜70.1%,アミノ酸配列で64,1〜70.0%と低く,ニホンジカのタイレリアには遺伝的に異なる株が存在することが示唆された.  The 18S rRNA gene and the piroplasm major immunodominant protein gene (p33/34) of Theileria from various subspecies of sika deer in 8 different locations of Japan were analyzed. The similarity between 633 bp partial sequences of the 18S rRNA gene among various subspecies of sika deer was found to be between 99.7% and 100%. While the percent identities of the 412 bp partial p33/34 gene sequence and deduced amino acid sequences between Theileria of sika deer from Yamaguchi Prefecture and those found in deer from other Prefectures, were comparatively low, 68.7% to...
Palavras-chave: Cervue nipon; Divergence; Theileria sp.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4004

めん羊と山羊の主な疾病について
Provedor de dados: 19
Autores: 猪熊, 壽; INOKUMA, Hisashi.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4387

Slow recovery of blood glucose in the insulin tolerance test during the prepartum transition period negatively impacts the nutritional status and reproductive performance postpartum of dairy cows.
Provedor de dados: 19
Autores: LEE, Hsu-Hsun; KIDA, Katsuya; MIURA, Ryotaro; INOKUMA, Hisashi; MIYAMOTO, Akio; KAWASHIMA, Chiho; HANEDA, Shingo; MIYAKE, Yoh-Ichi; MATSUI, Motozumi; 猪熊, 壽; 宮本, 明夫; 川島, 千帆; 羽田, 真悟; 松井, 基純.
In peripartum dairy cows, insulin resistance (IR) increases to adjust the direction of energy to lactation after calving. To investigate the effect of prepartum IR on postpartum reproductive performance, the insulin tolerance test (ITT) was applied to 15 cows at 3 weeks (Pre21) and 10 days (Pre10) before the predicted calving date. Blood glucose area under the curve (AUCglu) within 120 min after administration of 0.05 IU/kg-BW insulin was calculated. The occurrence of first ovulation, days to first artificial insemination (AI) and first AI conception rate were recorded. Nutritional status postpartum was evaluated by blood chemical analysis. Based on AUCglu changes from Pre21 to Pre10, cows were classified into either the AUC-up group (AUCglu increase, n=5)...
Palavras-chave: Dairy cows; Glucose metabolism; Insulin tolerance test; Reproductive performance; Transition period.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4129

MDCT Images of the Head of a Horse with Malignant Melanoma
Provedor de dados: 19
Autores: SASAKI, Naoki; MINAMI, Takuto; YAMADA, Kazutaka; SATOH, Motoyoshi; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; YAMADA, Haruo; 佐々木, 直樹; 山田, 一孝; 猪熊, 壽; 古林, 与志安; 古岡, 秀文.
A 17-year-old, gray Thoroughbred gelding was presented with recurrent epistaxis following exercise for over one year. Multidetector-row computed tomographic (MDCT) examination of the head performed under general anesthesia indicated the presence of malignant melanoma. MDCT examination revealed the exact location and size of masses in the right guttural pouch first identified by endoscopy and in the left guttural pouch unidentified by endoscopy. MDCT examination revealed the presence of a blood clot that filled the right maxillary sinus. The horse was euthanized due to poor prognosis as a riding horse and the owner's decision, and MDCT findings were confirmed by postmortem examination. MDCT is suggested as a useful technique for diagnosis of head problems...
Palavras-chave: Equine; Malignant melanoma; MDCT.
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3104

ポータブルX線撮影装置を用いた牛の Computed Radiography 画像化因子が画質に与える影響
Provedor de dados: 19
Autores: 岸本, 海織; 住谷, 峻; 李, 奇子; 清水, 純一郎; 山川, 和宏; 滄木, 孝弘; 羽田, 真悟; 松本, 高太郎; 石井, 三都夫; 佐々木, 直樹; 猪熊, 壽; 山田, 一孝; KISHIMOTO, Miori; SUMIYA, Takashi; LEE, Ki-Ja; SHIMIZU, Junichiro; YAMAKAWA, Kazuhiro; AOKI, Takahiro; HANEDA, Shingo; MATSUMOTO, Kotaro; ISHII, Mitsuo; SASAKI, Naoki; INOKUMA, Hisashi; YAMADA, Kazutaka.
牛臨床におけるComputed Radiography (CR) の有用性を確立するため，管球-カセッテ間距離およびImaging Plate (IP) の読み取り時間が画質に与える影響について検討した.管球-カセッテ間距離は画質の視覚的スコア(粒状性，鮮鋭性)に影響しなかった. IPの読み取り時間は画質の鮮鋭性と相関しなかったが，粒状性と相関が認められた.しかし読み取り時聞が12時間以内の場合には問題にならない程度であると考えられた.管球-力セッテ間距離を再現よく保つことが難しく， IP読み取り時間のばらつきが想定される牛の臨床画像診断においてCRは有用なX線撮影システムであると考えられた. The purpose of this study was to investigate the effect of X-ray tube-cassette distance and image scanning time on image quality to establish the benefit of Computed Radiography (CR) in bovine clinical practice. The tube-cassette distance had no effect on the visual evaluation score (graininess and sharpness). The image scanning time correlated with graininess but not sharpness. From these results，it was concluded that accidental variations in the tube-cassette...
Palavras-chave: 牛; Computed Radiography (CR); フェーデイング; Imaging Plate (IP); ポータブルX線発生装置; Bovine; Computed Radiography (CR); Fading; Imaging Plate (lP); Portable x-ray generator.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2939

慢性子宮内膜炎を呈したホルスタイン種雌牛にみられた牛白血病の1症例
Provedor de dados: 19
Autores: 竹内, 俊彦; 吉本, 薫; 駒形, 真; 福中, 守人; 古林, 与志安; 松本, 高太郎; 猪熊, 壽; TAKEUCHI, Toshihiko; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; FUKUNAKA, Morito; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi.
5歳5カ月齢のホルスタイン種雌牛が，発熱，食欲不振及び胎盤停滞を呈した．悪露排出及び子宮の腫脹は治療で改善がみられなかった．体表リンパ節の腫大はみられなかったが，直腸検査で子宮の硬結を認めたため，牛白血病を疑った．病理解剖では腹腔内リンパ節の腫大，胃漿膜面の腫瘤形成及び子宮壁の肥厚が認められた．また，組織所見ではこれら組織へのリンパ球様腫瘍細胞の浸潤が認められた．腫瘍細胞はB細胞マーカー陽性であった．牛白血病ウイルス（BLV）抗体は陰性であったが，末梢血のBLVプロウイルス検査では陽性が確認され，本症例におけるBLVの関与が示唆された． A 5-year-5-month old Holstein cow was presented with fever, anorexia, and retained placenta. Postnatal vaginal discharge and enlargement of the uterus did not improve after treatment. Although the superficial lymph nodes were not enlarged, enzootic bovine leukosis was suspected because of the enlarged and indurated uterus. An autopsy revealed enlargement of most abdominal lymph nodes, tumor mass formation on the omasum and abomasum, and thickening of the uterine wall. Histologically, there...
Palavras-chave: 牛白血病ウイルス; 慢性子宮内膜炎; 地方病性牛白血病; Bovine leukosis virus; Chronic endometritis; Enzootic bovine leukosis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3555

ウマの胃排出能に対するクエン酸モサプリドの促進効果(内科学)
Provedor de dados: 19
Autores: OKAMURA, Koichi; SASAKI, Naoki; FUKUNAKA, Morito; YAMADA, Haruo; INOKUMA, Hisashi; 佐々木, 直樹; 猪熊, 壽.
The prokinetic effect of the 5-HT4 receptor agonist mosapride was evaluated in seven healthy thoroughbreds. Mosapride was orally administerd at doses of 0.5, 1.0 or 1.5 mg/kg. The breath 13CO2/12CO2 rate (Δ13CO2), an indirect indicator for the rate of gastric empting, was measured at appropriate points for 4 hr after drug administration. There was a significant increase compared with the control value at 15, 20 and 165 min for 0.5 mg/kg, 30 min at 1.0 mg/kg and 165 min for 1.5 mg/kg. The results suggest that mosapride may facilitate the gastric emptying in horses.
Palavras-chave: Equine; Gastric emptying rate; Mosapride citrate.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4019

Chronic interstitial nephritis with edema in a Holstein heifer
Provedor de dados: 19
Autores: 髙橋, 英二; 竹内, 俊彦; 丸山, 亮介; 古林, 与志安; 猪熊, 壽; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
1歳4カ月齢のホルスタイン種育成牛が発育不良を主訴に受診した．当該牛は体高110cmで，著しい削痩を呈していた．臨床検査により，非再生性貧血，高窒素血症，高クレアチニン血症，高リン血症，低カルシウム血症，低比重尿および蛋白尿が認められ，慢性腎不全が疑われた．輸液により治療を試みたが，第15病日には下顎と胸垂の浮腫が発現し，第16病日に死亡した．病理学的検索の結果，糸球体病変を伴う慢性間質性腎炎と診断されたが，原因は不明であった．
Palavras-chave: 慢性間質性腎炎; 浮腫; ホルスタイン育成牛.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4366

右心房内に血栓を認めた後大静脈血栓症の育成牛の1 例
Provedor de dados: 19
Autores: 猪熊, 壽; 吉林台; 下田, 崇; 富樫, 義彦; 古林, 与志安; 古岡, 秀文; 佐藤, 基佳; 石井, 三都夫; INOKUMA, Hisashi; Jilintai; SHIMODA, Takashi; TOGASHl, Yoshihiko; KOBAYASHl, Yoshiyasu; FURUOKA, Hidefumi; SATHO, Motoyoshi; ISHI, Mitsuo.
1 歳10 カ月のホルスタイン種育成雌牛が，初診時食欲不振，削痩，チアノーゼ，肺音粗励，発咳等の症状を呈した． さらに第14病日には頻脈，頸静脈拍動等の循環器症状が認められた．血液および血液生化学検査により慢性炎症像が， また心エコー検査により右心房内に三尖弁と付着しない高エコー占拠性病変が観察された．本症例は病理解剖により後 大静脈血栓症と診断されたが，血栓が心臓内において描出されたまれな症例と思われた． A 22-month-old，Holstein heifer was presented with anorexia，emaciation，cyanosis，rough vesicular breathing， and coughing on the first day. On day 14， cardiovascular symptoms such as tachycardia and jugular vein pulsation were found. A blood and blood biochemical examination revealed chronic inflammation. High echogenic obstruction without any attachment to the tricuspid valve was observed in the right atrium by echocardiograph. Pathological findings confirmed a diagnosis of caudal vena cava thrombosis. It was believed to be a rare case with thrombus in the...
Palavras-chave: 後大静脈血栓症; 心エコー検査; Caudal vena cava thrombosis; Echocardiography.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2805

完全大血管転位が認められたホルスタイン種牛の1症例
Provedor de dados: 19
Autores: 佐々木, 広美; 下田, 崇; 古林, 与志安; 猪熊, 壽; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
Palavras-chave: 完全大血管転位; 心室中隔欠損; ホルスタイン種.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4338

多発性乳房膿瘍に罹患したホルスタイン種乳牛にみられた左心系心内膜炎の１症例
Provedor de dados: 19
Autores: 猪熊, 壽; 秋月, 久美子; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi.
３歳１０カ月のホルスタイン種雌乳牛に、右後肢跛行、発熱及び多発性乳房腫瘤が認められた。抗菌薬と解熱 鎮痛剤による治療に反応せず、第１８病日には心雑音が発現した。心音図解析により心雑音は拡張期雑音と確認 され、また心エコー検査により大動脈弁の疣贅性心内膜炎が疑われた。病理解剖により、僧帽弁と大動脈弁の 疣贅性心内膜炎が認められた。肺及び乳房割面には多数の膿瘍を認め、右腎と脾臓には梗塞部が散在していた。 本症例では、左心系に疣贅性心内膜炎が生じた結果、多発性乳房膿瘍および腎臓と脾臓の梗塞が継発したと考 えられた。
Palavras-chave: 疣贅性心内膜炎; 僧帽弁; 大動脈弁; 多発性膿瘍; ホルスタイン種.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4032

Prevalence and Risk Factor Analysis of Bovine Hemoplasma Infection by Direct PCR in Eastern Hokkaido, Japan
Provedor de dados: 19
Autores: TAGAWA, Michihito; YBANEZ, Adrian P.; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
The prevalence and risk factors of 2 bovine hemoplasma species—Mycoplasma wenyonii (Mw) and “Candidatus Mycoplasma haemobos” (CMh)—were examined by direct PCR using whole blood samples collected from 343 cattle in Eastern Hokkaido, Japan. The sensitivity of the direct PCR assay was 10-fold higher than the standard PCR assay, detecting 5 copies of bovine hemoplasma DNA per reaction. An epidemiological survey of sampling location, living conditions, age and bovine leukemia virus antibody was performed to assess infection risk factors. The prevalence rates were 38.5% for Mw and 39.1% for CMh, respectively, with an overall prevalence rate of 64.7% for all bovine hemoplasma infections. Significant differences were found in sampling location, living conditions...
Palavras-chave: Bovine hemoplasma; Direct PCR; Japan; Risk factor.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3838

Evaluation of Gene Expression in Peripheral Blood Cells as a Potential Biomarker for Enzootic Bovine Leukosis
Provedor de dados: 19
Autores: TAWFEEQ, Mohammad Monir; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽.
Expression of six selective genes in peripheral blood cells was evaluated as diagnostic biomarkers for enzootic bovine leukosis (EBL) by using 10 EBL and 15 clinically healthy cattle. The clinically healthy cattle generally showed lower gene expression levels. Although wide variations of gene expression were found in some clinical cases of EBL, 4 and 5 among 10 EBL cattle showed higher expression of interleukin 2 receptor gene (IL2R) and Wilms’ tumor gene (WT1), respectively. Expression of IL2R in peripheral blood cells in EBL cattle was statistically increased; however, the lower sensitivity and higher variation in the gene expressions among clinical cases of EBL would be problems as diagnostic biomarkers.
Palavras-chave: Biomarkers; Enzootic bovine leukosis; Gene expression; QRT-PCR.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3868

脳幹部膿瘍により神経症状を呈したホルスタイン種子牛の1 例
Provedor de dados: 19
Autores: 松本, 高太郎; 村上, 智亮; 菅生, 樹春; 山田, 一孝; 古林, 与志安; 松井, 高峯; 猪熊, 壽; MATSUMOTO, Kotaro; MURAKAMI, Tomotaka; SUGO, Kiharu; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; MATSUI, Takane; INOKUMA, Hisashi.
17日齢のホルスタイン種雌子牛が，神経症状を示した. 病畜は頭部を左後方に折り曲げて伏臥姿勢を示し，時折自力で起立したがすぐに倒れ歩行不能であった.　神経学的検査では右の対光反射，威嚇瞬き反射，眼瞼反射， および音響耳介反射が消失していた.　ビタミンB1，およびデキサメサゾンの投与により第7病日に歩行可能となったものの左への斜頸・旋回が認められ，神経学的検査所見に改善は認められなかった.　第7病日に病理解剖を行い，肉眼的には橋の断面右側に直径1cm大の膿瘍を認め，嫌気培養にてFusobacterium necrophorumが検出された.　左側への斜頸，聴覚の消失等の脳神経疾状は，橋の右側の膿瘍により内耳神経等の脳神経核が圧迫された結果出現した聴覚障害，平衡障害のためと考えられた. A 17-day-0ld female Holstein calf exhibited the following neurological symptoms: the calf bent its neck to the left，turning its face backward and could stand alone，but would fall within a short time. Neurological examination further revealed losses of pupillary light reflex， menace response，palpebral reflex，and hearing on the right side. After administration of dexamethasone and vitamin B1，the calf was able to walk...
Palavras-chave: 脳幹部膿瘍; 牛; Fusobacterium necrophorum; Brain stem abscess; Cattle.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2945

三尖弁に大型疣贅物を形成したホルスタイン種乳牛の１症例
Provedor de dados: 19
Autores: 小島, 由夏; 上野, 拓; 三島, 貴吉備; 古林, 与志安; 猪熊, 壽; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
Palavras-chave: 疣贅性心内膜炎; ホルスタイン種; 心臓超音波検査.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4258

Overexpression of Interleukin 2 Receptor, Thymidine Kinase and Immunoglobulin-Associated Alpha-1 Messenger RNA in a Clinical Case of Enzootic Bovine Leukosis
Provedor de dados: 19
Autores: TAWFEEQ, Mohammad Monir; TAGAWA, Michihito; ITOH, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽.
A 49-month-old Holstein cow with anorexia, tachypnea, enlarged peripheral lymph nodes, and difficulty standing up was suspected of bovine leukosis. Hematological examination revealed lymphocytosis with the presence of neoplastic cells. Increased total lactate dehydrogenase (LDH) activity, isozymes of LDH-2 and LDH-3 activities and thymidine kinase activity were observed. Cytological findings of fine needle aspiration of subiliac lymph nodes indicated lymphosarcoma. Histopathology and antibody analysis confirmed the diagnosis of enzootic bovine leukosis, a B-cell bovine lymphoma caused by bovine leukemia virus. Gene expressions known as biomarkers of hematopoietic neoplasia in human were also examined in the present case. Increased messenger RNA expression...
Palavras-chave: Biomarker; Bovine leukosis; Gene expression.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4096

Clinical Case of a Brainstem Tumor in a Holstein Cow
Provedor de dados: 19
Autores: 三浦, 沙織; 千葉, 史織; 高橋, 裕之; 福田, 茂夫; 藤井, 貴志; 新井, 鐘蔵; 古林, 与志安; 猪熊, 壽; MIURA, Saori; CHIBA, Shiori; TAKAHASHI, Hiroyuki; FUKUDA, Shigeo; FUJII, Takashi; ARAI, Shozo; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
38カ月齢のホルスタイン種乳牛において，体全体が左側に傾くという神経症状が進行性に認められた．経過，左側対光反射と左側音響耳介反応の減弱，及び聴性脳幹誘発電位測定検査における左刺激時Ⅲ波-Ⅴ波の遅延所見より，延髄から橋における片側性圧迫性病変を疑った．病理解剖の結果，橋左側に帯黄色脆弱化病巣を認め，病理組織学的検索により，病変は腫瘍であり，迷入組織に由来する可能性があるものの，原発組織は確定できなかった．
Palavras-chave: 聴性脳幹誘発電位; 脳幹; 腫瘍; ホルスタイン.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4010

北海道東部の放牧場における小型ピロプラズマ感染リスク低減要因の検討
Provedor de dados: 19
Autores: 猪熊, 壽; 横山, 直明; INOKUMA, Hisashi; YOKOYAMA, Naoaki.
北海道東部(道東)の放牧地における牛の小型ピロプラズマ感染りスク低減要因について調査した。道東ではシュルツェマダニ，ヤマトマダニ，ダグラスチマダニが採取され， PCRにより小型ピロプラズマ遺伝子断片が検出され，これらのマダニがベクターとなりうる可能性が考えられた。エゾシカからは多数のマダニが回収され，小型ピロプラズマのベクターを牛の放牧地内へ持ち込むという意味で小型ピロプラズマ感染リスクに関わる動物と考えられた。また，放牧管理体制との関連では，野生動物侵入防止柵の設置，あるいは牧柵周辺の下草刈りが小型ピロプラズマ感染リスク低減に有効である可能性が考えられた。 Factors to decrease risk for Theileria orientalis infection of cattle in pastureland in Eastern Hokkaido were investigated. Ixodes persulcatus，Ixodes ovatus and Haemaphysalis douglusii were collected from the vegetation of the pastureland，and DNA　fragments of T. orientalis were detected from these ticks by using PCR. The results suggested that these tick species may be vectors for T. orientalis in this area. Many ticks have been recovered from wild sika deer，and it was thought...
Palavras-chave: 道東; リスク低減要因; Theileria orientalis; Eastern Hokkaido; Factors to decrease risk.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2790

Effect of Chronic Hemoplasma Infection on Cattle Productivity
Provedor de dados: 19
Autores: TAGAWA, Michihito; YAMAKAWA, Kazuhiro; AOKI, Takahiro; MATSUMOTO, Kotaro; ISHII, Mitsuo; INOKUMA, Hisashi; 滄木, 孝弘; 松本, 高太郎; 石井, 三都夫; 猪熊, 壽.
The present study evaluated the effect of hemoplasmosis on cattle productivity. Prevalence of bovine hemoplasma was examined by polymerase chain reaction (PCR) using whole blood samples collected from 93 breeding cows and their 71 calves in Hokkaido, Japan. Monthly milk production records and other clinical data were compared between Mycoplasma wenyonii (Mw)-infected, “Candidatus Mycoplasma haemobos” (CMh)-infected, co-infected and PCR-negative groups. Blood chemical parameters were obtained from the 93 cows and 64 calves. PCR results showed that 89.2% (83/93) of cows and 14.1% (10/71) of calves were positive for bovine hemoplasma. Based on productivity data obtained from the 93 cows, Mw-infected, CMh-infected and co-infected cows had significantly lower...
Palavras-chave: Cattle; Direct PCR; Hemoplasma; Productivity.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3870

Periventricular leukomalacia in a neonatal calf
Provedor de dados: 19
Autores: KOYAMA, Kenji; FUJITA, Riku; MAEZAWA, Masaki; FUKUMOTO, Natsuko; HORIUCHI, Noriyuki; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 猪熊, 壽; 古林, 与志安.
A 10-day-old, Japanese Black, female calf had shown astasia since just after birth. Focal symmetrical periventricular malacic lesions of the cerebrum and suppurative arthritis of the left hip joint were observed in macroscopic examination. Histologically, the cerebral lesions were confirmed as periventricular leukomalacia (PVL). The location and histological features of the lesions were similar to PVL in humans, caused by neonatal ischemia/hypovolemia. This is the first report of PVL in a neonatal calf.
Palavras-chave: Ischemia; Neonatal calf; Periventricular leukomalacia.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4324

Molecular Epidemiological Survey of Theileria orientalis in Thua Thien Hue Province, Vietnam
Provedor de dados: 19
Autores: KHUKHUU, Altangerel; LAN, Dinh Thi Bich; LONG, Phung Thang; UENO, Akio; LI, Yan; LUO, Yuzi; MACEDO, Alan Caine Costa de; MATSUMOTO, Kotaro; INOKUMA, Hisashi; KAWAZU, Shin-Ichiro; IGARASHI, Ikuo; XUAN, Xuenan; YOKOYAMA, Naoaki; 松本, 高太郎; 猪熊, 壽; 河津, 信一郎; 五十嵐, 郁男; 玄, 学南; 横山, 直明.
Theileria orientalis is a benign bovine protozoan parasite that occasionally causes serious economic loss in the livestock industry. We report the findings of a molecular epidemiological survey of T. orientalis in 94 Vietnamese yellow cattle, 43 water buffaloes, 21 sheep, 21 goats and 85 blood-sucking ticks of cattle in the Thua Thien Hue province of Vietnam. The major piroplasm surface protein (MPSP) gene of T. orientalis was detected using polymerase chain reaction from 13 cattle (13.8%), 11 water buffaloes (25.6%), 1 sheep (4.8%) and 9 ticks (10.6%). Phylogenetic analysis using MPSP gene sequences showed the presence of seven genotypes, four previously categorized genotypes (Types 1, 3, 5 and 7) and three new genotypes (Types N-1, N-2 and N-3).
Palavras-chave: Cattle; Genotype; MPSP; Theileria orientalis; Vietnam.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3585

Brain Abscess in a Japanese Black Calf : Utility of Computed Tomography (CT)
Provedor de dados: 19
Autores: EL-KHODERY, Sabry; YAMADA, Kazutaka; AOKI, Daisuke; KAMIO, Kyouhei; KISHIMOTO, Miori; SHIMIZU, Junichiro; KOBAYASHI, Yoshiyasu; ISHII, Mitsuo; INOKUMA, Hisashi; YAMAUCHI, Sen-ichi; MATSUI, Takane; 山田, 一孝; 古林, 与志安; 石井, 三都夫; 猪熊, 壽.
Computed tomography (CT) was used for diagnosis of brain abscess in a 6-month-old, Japanese black calf presented with neurological dysfunction, compulsive circling and vision disturbance. CT images showed asymmetric lateral ventricles, and presence of intra-cranial multiple low absorption lesions surrounded by capsule suggestive of abscess in the right cerebral hemisphere. Postmortem examination revealed marked swelling of right cerebral hemisphere and olfactory bulb. Multilocular large abscess containing creamy pus was found to occupy most area of periventricular and lateral ventricle. Fusobacterium necrophrum was isolated from the abscess contents as the causative agent. These results demonstrate that CT is useful tool for tentative diagnosis of bovine...
Palavras-chave: Bovine; Brain abscess; CT.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3926

左右房室の疣贅性心内膜炎を認めたホルスタイン種乳牛の1症例
Provedor de dados: 19
Autores: 上坂, 花鈴; 藤田, 理公; 水島, 仁人; 堀内, 雅之; 古林, 与志安; 川本, 恵子; 猪熊, 壽; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; KAWAMOTO, Keiko; INOKUMA, Hisashi.
3歳6カ月齢のホルスタイン種乳牛が、分娩2カ月後に食欲不振を主訴に受診した。第6病日には肺音粗励 と心音混濁が確認され、心内膜炎を疑った。第13病日の血液生化学検査では慢性炎症像が確認され、また心音 心電図解析の結果、Ⅰ音分裂と延長、および二峰性P波がみられた。さらに心臓超音波検査では、僧帽弁の疣 贅物と僧帽弁逆流、左心室と左心房の拡張が認められた。第16病日に実施された病理解剖では、僧帽弁に3× 2×2cm 大、三尖弁に3×1×1cm大の疣贅物がそれぞれ確認されたほか、腎梗塞および左肩と左膝の関節 炎がみられた。細菌学的検索では疣贅物、肺、腎臓、関節からは細菌が分離されず、心内膜炎とその他病変と の関係性を解明することができなかった。
Palavras-chave: 疣贅性心内膜炎; 三尖弁; 僧帽弁.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4278

乳牛の血様液貯留を特徴とする特発性心嚢水腫症例と心膜炎症例の臨床病理学的検査所見の比較
Provedor de dados: 19
Autores: 猪熊, 壽; 滄木, 孝弘; 山川, 和宏; 竹内, 俊彦; 松本, 高太郎; 石井, 三都夫; 古林, 与志安; 古岡, 秀文; INOKUMA, Hisashi; AOKI, Takahiro; YAMAKAWA, Kazuhiro; TAKEUCHI, Toshihiko; MATSUMOTO, Kotaro; ISHII, Mitsuo; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi.
病理学的に血様液貯留を特徴とする特発性心嚢水腫と診断された乳牛8症例（血様心嚢水貯留群）の臨床病理学的検査所見を回顧的に取りまとめ，心膜炎7症例（心膜炎群）の所見と比較した．血様心嚢水貯留群では冷性浮腫を呈する症例が心膜炎群に比べて多かったが，その他の臨床症状には両群で差がなかった．心エコー検査では血様心嚢水貯留群では，心膜炎でみられる心嚢内の顕著なフィブリン析出はなかった．血液及び生化学検査では，血様心嚢水貯留群では炎症像が弱く，心膜炎群と比較して白血球数，分葉核好中球割合，A/G比，アルブミン濃度，γグロブリン割合に有意差がみられた．両群でALP及びGGTの高値がみられたが，特に血様心嚢水貯留群のGGT増加は顕著であった．血様心嚢水貯留症例の診断は臨床症状だけでは困難であるが，心エコー検査所見，弱い炎症像，ALP及びGGTの高値などが心膜炎との鑑別診断に有用と考えられた． Clinicopathological findings of eight cases of idiopathic hydropericardium characterized by bloodhlike fluid (HBFhgroup) that were confirmed by necropsy were retrospectively summarized and compared with those of seven cows with pericarditis. Although the numbers of cows that showed edema in the HBFhgroup were larger than those in the pericarditishgroup, the frequency of...
Palavras-chave: 特発性心餒水腫; 心膜炎; 血様心嚢水; 牛; Idiopathic hydropericardium; Pericarditis; Blood-like pericardium; Cow.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3557

犬 Babesia gibsoni 感染症の発生状況に関する全国アンケート調査
Provedor de dados: 19
Autores: 猪熊, 壽; 田井, 貴子; 市川, 康明; INOKUMA, Hisashi; TAI, Takako; ICHIKAWA, Yasuaki.
全国47都道府県の小動物診療施設を対象に2009年（09年）と2010年（10年）の犬Babesia gibsoni感染症発生状況に関するアンケート調査を実施した．9,513施設か6,746 の回答が得られ（回収率70.9 ％），うち859施設（12.7 ％）が本症を経験していた．確定症例数は09年3,802，10年3,625で，うち東日本では09年87，10年75であった．栃木，茨城，群馬，埼玉，東京，長野の関東以北6都県では，西日本への移動又は旅行歴のない，闘犬以外の品種9頭に確定症例が認められ，関東以北でのB. gibsoni自然感染が示唆された．西日本では769 施設で09年3,715，10年3,550の確定症例があり，特に香川，熊本，徳島，山口，福岡，宮崎，兵庫各県で，年間200を超える症例が報告された． A nationwide questionnaire was conducted on the prevalence of the Babesia gibsoni infection in dogs in 2009 and 2010. The questionnaire was sent to 9,513 animal hospitals in all 47 prefectures, and 6,746 answers (70.9%) were received. Among the respondents, 859 animal hospitals (12.7%) have diagnosed B. gibsoni infection in dogs. The numbers of patients were 3,802 and 3,625 in 2009 and 2010,...
Palavras-chave: Babesia gibsoni; 犬; 日本; アンケート調査; 発生状況; Dogs; Japan; Questionnaire; Surveillance.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3556

クリイロコイタマダニの唾液腺抽出物が犬末梢血リンパ球の免疫グロブリンクラス産生能におよぼす影響(短報)
Provedor de dados: 19
Autores: MATSUMOTO, Koutaro; INOKUMA, Hisashi; OHNO, Koichi; ONISHII, Takafumi; 松本, 高太郎; 猪熊, 壽.
Effects of salivary gland extract(SGE) form Rhipicephalus sanguineus on immunoglobulin class productivity of canine peripheral blood lymphocytes(PBL)in vitro were studied. The detectable limit of the ELISA for canine total immunoglobulin.IgM and A was at least 1, 1 and 15 ng/ml, respectively, and it seems to be useful for the evaluation of non-specific immunoglobulin class productivity in vitro. SGE from R. sanguineus suppressed pokeweed mitogen-or lipopolysaccharide total immunoglobulin and IgA productivity of canine PBL although IgM productivity was not suppressed. These results suggested that the suppression was caused partly by the direct effect of SGE on B lymphocytes.
Palavras-chave: Canine; Immunoglobulin class productivity; Rhipicephalus sanguineus.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4174

Molecular Diagnosis of Infective Endocarditis Caused by Streptococcus canis in a Dog
Provedor de dados: 19
Autores: 田川, 道人; 大橋, 英二; 本村, 絹代; 千葉, 史織; 堀内, 雅之; 三好, 雅史; 猪熊, 壽; 松本, 高太郎; TAGAWA, Michihito; OHASHI, Eiji; MOTOMURA, Kinuyo; CHIBA, Shiori; HORIUCHI, Noriyuki; MIYOSHI, Masahumi; INOKUMA, Hisashi; MATSUMOTO, Kotaro.
10歳の雄の雑種犬が，3週間前から持続する発熱及び元気消失を主訴に帯広畜産大学動物医療センターを受診した．心雑音及び心エコー検査において僧帽弁の疣贅物が確認され，抗生物質等により加療したが，第7病日より心不全の悪化を認め，第10病日に肺水腫により斃死した．病理解剖では，軽度の心拡大及び僧帽弁における多中心性疣贅物が認められた．血液培養検査では，Staphylococcus haemolyticusが検出されたが，疣贅物を用いたPCR検査ではStreptococcus canisと100％一致する塩基配列が得られた．血液培養分離菌はコンタミネーションであった可能性が高く，本症例の心内膜炎はStreptococcus canisによるものと考えられた．血液培養結果と実際の心内膜炎起因菌が異なる場合があることを考慮する必要がある．
Palavras-chave: 心内膜炎; PCR; Streptococcus canis.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4232

Evaluation of S100B in cerebrospinal fluid as a potential biomarker for neurological diseases in calves
Provedor de dados: 19
Autores: KOJIMA, Yuka; CHIBA, Shiori; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 猪熊, 壽.
S100B in cerebrospinal fluid (CSF-S100B) was measured in calves with 20 neurologic and 21 non-neurologic diseases to clarify its utility as a biomarker for neurologic diseases. The median CSF-S100B value in the neurologic disease group (43.0 ng/ml) was significantly higher than that in the non-neurologic disease group (10.2 ng/ml). As CSF-S100B levels in calves with neurologic diseases widely differed, the utility of CSF-S100B as a diagnostic marker for neurologic diseases in cattle remains inconclusive.
Palavras-chave: Calf; Cerebrospinal fluid; S100B.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4142

Prevalence and Molecular Analyses of Hemotrophic Mycoplasma spp. (Hemoplasmas) Detected in Sika Deer (Cervus nippon yesoensis) in Japan
Provedor de dados: 19
Autores: TAGAWA, Michihito; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Hemotropic mycoplasmas (hemoplasmas) are cell-wall deficient, epierythrocytic bacteria that cause infectious anemia in several mammalian species. The prevalence of hemoplasma species was examined by screening and species-specific PCR using blood samples collected from 51 sika deer in Hokkaido, Japan. Molecular analyses were performed for the 16S rRNA, 23S rRNA and RNase P RNA (rnpB) gene sequences. A total of 23/51 (45%) deer DNA samples were positive for hemoplasmas in the screening PCR. Using species-specific PCR, 12 and 17 samples were positive for ‘Candidatus Mycoplasma haemocervae’ and ‘Candidatus M. erythrocervae’, respectively. Sequencing and phylogenetic trees of those three genes indicate that the ‘Candidatus M. haemocervae’ and ‘Candidatus M....
Palavras-chave: Cervus nippon yesoensis; Hemoplasma; Japan; Phylogeny.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4012

Zygomycotic Mediastinal Lymphadenitis in Beef Cattle with Ruminal Tympany
Provedor de dados: 19
Autores: NISHIMURA, Maki; TOYOTA, Yumi; ISHIDA, Yoshikazu; NAKAYA, Hideki; KAMEYAMA, Kyohko; NISHIKAWA, Yoshifumi; MIYAHARA, Kazuro; INOKUMA, Hisashi; FURUOKA, Hidefumi; 西川, 義文; 猪熊, 壽; 古岡, 秀文.
A 9-month-old steer was autopsied due to recurrent ruminal tympany. A macroscopic examination found an enlarged caudal mediastinal lymph node, and a section of the lymph node revealed necrosis with marked calcification, similar to tuberculous lymphadenitis. Histopathologically, the lesion consisted of multiple coagulative necrotic foci and fibrosis with macrophage, lymphocyte, eosinophil and multinucleated giant cell infiltration. Non-uniform width hyphae were detected in the necrotic area and within the cytoplasm of the multinucleated giant cells, and they were found to be anti-Rhizopus arrhizus antibody positive in an immunohistochemical examination. Therefore, the steer was diagnosed with necrotic caudal mediastinal lymphadenitis due to zygomycetes...
Palavras-chave: Cattle; Necrotic lymphadenitis; Ruminal tympany; Zygomycosis.
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3943

Specific Molecular Detection and Characterization of Anaplasma marginale in Mongolian Cattle
Provedor de dados: 19
Autores: YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; BATTSETSEG, Badgar; BATTUR, Banzragch; ALTANGEREL, Khukhuu; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma marginale is an etiologic agent of bovine anaplasmosis. This study aimed to molecularly detect and characterize A. marginale that is prevalent in Mongolian cattle populations. A highly specific and sensitive nested PCR (nPCR) method based on the Msp5 gene was developed to detect A. marginale (Msp5 nPCR). The method detected A. marginale from the positive DNA samples obtained from different countries, while no amplicons were observed from DNA samples of several other bovine blood pathogens tested. The detection limit of Msp5 nPCR was determined to be 2 copies/μl. The method was tested against field blood DNA samples prepared from 300 Mongolian cattle in 2010. Results indicated a prevalence rate of 8.7% (26 of 300). On the other hand, partial DNA...
Palavras-chave: Anaplasma marginale; Cattle; Mongolia; Msp5; PCR.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3867

マダニとマダニ媒介性疾患 : アップデート
Provedor de dados: 19
Autores: 猪熊, 壽; INOKUMA, Hisashi.
特集：第9回大動物臨床研究会特別セミナー 再興疫病 ピロプラズマ病を考える
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4386

Glomus tumor of the liver in a cow
Provedor de dados: 19
Autores: HORIUCHI, Noriyuki; KOMAGATA, Makoto; SHITAMURA, Keiichi; CHIBA, Shiori; MATSUMOTO, Kotaro; INOKUMA, Hisashi; MATSUI, Takane; KOBAYASHI, Yoshiyasu; 堀内, 雅之; 松本, 高太郎; 猪熊, 壽; 古林, 与志安.
An 11-year-old Holstein-Friesian cow exhibited anorexia and jaundice. A large mass was found in the liver during necropsy. Macroscopically, the mass was composed of dark red multilobular tissue and a centrally located abscess, which was connected to the hepatic duct. Histologically, the mass consisted of proliferation of small neoplastic cells and was demarcated from the hepatic parenchyma by a thick region of granulation tissue. The neoplastic cells were predominantly arranged in solid sheets, but they also formed blood-filled cancellous structures, and proliferating foci were seen around blood vessels. Periodic acid-Schiff reaction demonstrated that a fine basement membrane-like structure surrounded the neoplastic cells. Immunohistochemically, the...
Palavras-chave: Cholangitic abscess; Cow; Glomus tumor.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4172

Three Cases of Atypical Bovine Leukosis in Holstein Cows
Provedor de dados: 19
Autores: 田川, 道人; 下田, 崇; 富樫, 義彦; 渡辺, 由紀; 古林, 与志安; 古岡, 秀文; 石井, 三都夫; 猪熊, 壽; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; ISHII, Mitsuo; INOKUMA, Hisashi.
体表リンパ節の腫脹, 末梢血中における異型リンパ球出現を伴うリンパ球数の著増などの典型的な牛白血病症状を示していなかったことから, それぞれアミロイドーシス, 胸膜炎, 心疾患が疑われた3頭のホルスタイン種乳雌牛を病理解剖したところ, いずれも牛白血病と診断された. 血液検査および身体検査において著変が観察されない場合でも, 難診断時には鑑別診断リストに牛白血病を加えるべきであると考えられた.
Palavras-chave: 非典型的牛白血病; 鑑別診断.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4005

難診断感染症診断法確立を目指した新しい産業動物臨床教育研究システムの構築
Provedor de dados: 19
Autores: 猪熊, 壽; 古岡, 秀文; 度会, 雅久; 佐々木, 直樹; 石井, 三都夫; INOKUMA, Hisashi; FURUOKA, Hidefumi; SASAKI, Naoki; ISHII, Mitsuo.
プロジェクト研究の紹介 平成18〜19年度帯広畜産大学教育研究改革・改善プロジェクト研究の概要
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4158

First Molecular Characterization of Anaplasma marginale in Cattle and Rhipicephalus (Boophilus) microplus Ticks in Cebu, Philippines
Provedor de dados: 19
Autores: YBAÑEZ, Adrian Patalinghug; SIVAKUMAR, Thillaiampalam; YBAÑEZ, Rochelle Haidee Daclan; RATILLA, Jowarren Catingan; PEREZ, Zandro Obligado; GABOTERO, Shirleny Reyes; HAKIMI, Hassan; KAWAZU, Shin-ichiro; MATSUMOTO, Kotaro; YOKOYAMA, Naoaki; INOKUMA, Hisashi; 河津, 信一郎; 松本, 高太郎; 横山, 直明; 猪熊, 壽.
Anaplasma marginale has been detected in the Philippines only by peripheral blood smear examination and serological methods. This study generally aimed to molecularly detect and characterize A. marginale in cattle and ticks in Cebu, Philippines. A total of 12 bovine blood samples and 60 Rhipicephalus (Boophilus) microplus ticks were collected on the Cebu Island in 2011. 16S rRNA-based screening-PCR and DNA sequencing revealed 8 cattle (66.7%) and 8 ticks (13.3%) to be positive for A. marginale, and 1 tick (1.7%) to be positive for A. centrale. Selected positive DNA samples were further characterized based on 16S rRNA (longer sequence), Msp5, Msp1α,gltA and groEL genes for phylogenetic analyses. Sequence identities of partial DNA fragments of A. marginale...
Palavras-chave: Anaplasma marginale; Cattle; PCR; Philippines; Rhipicephalus (Boophilus) microplus.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3866

A Clinical Case of Maxillary Sinus Abscess in a Holstein Cow
Provedor de dados: 19
Autores: 髙橋, 英二; 山田, 一孝; 古林, 与志安; 猪熊, 壽; TAKAHASHI, Eiji; YAMADA, Kazutaka; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
7歳6カ月齢のホルスタイン種乳牛が顕著ないびき音を呈し，さらに左側頬骨部の一部で波動感を有する腫瘤が触知された．頭部X線検査により上顎洞内への液体貯留と上顎第3後臼歯の歯槽骨の融解が認められ，歯性上顎洞炎に継発する大型膿瘍であることが推察された．病理解剖により病変は上顎洞内膿瘍であることが明らかとなった．牛における頭部X線検査が上顎洞内膿瘍の診断及び病態の把握に有用であった．
Palavras-chave: ホルスタイン; 上顎洞内膿瘍; 歯性上顎洞炎.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4257

Utility of Serum Thymidine Kinase Activity Measurements for Cases of Bovine Leukosis with Difficult Clinical Diagnoses
Provedor de dados: 19
Autores: TAWFEEQ, Mohammad Monir; MIURA, Saori; HORIUCHI, Noriyuki; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 堀内, 雅之; 古林, 与志安; 古岡, 秀文; 猪熊, 壽.
This study evaluated the clinical usefulness of serum thymidine kinase (TK) activity for diagnosing bovine leukosis cases for which clinical diagnosis was difficult (‘BL with difficult diagnosis’). Median TK activity values in 24 ‘BL with difficult diagnosis’ and 36 cattle for which BL was clinically confirmed by cytology findings of enlarged superficial lymph nodes (‘clinically confirmed BL’) were 36.8 and 39.4 U/l, respectively (no significant difference). The percentage with positive TK activity (&gt;5.4 U/l) was also similar in both groups (83.3% for ‘BL with difficult diagnosis’ and 97.2% for ‘clinically confirmed BL’). TK activity was significantly higher in cows with ‘BL with difficult diagnosis’ compared to those with other tumors (N=13) and those...
Palavras-chave: Atypical bovine leukosis; Diagnosis; Thymidine kinase.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3869

脊柱管内膿瘍により後駆麻痺を呈したホルスタイン育成牛の１症例
Provedor de dados: 19
Autores: 竹内, 俊彦; 寒川, 彰久; 下タ村, 圭市; 下田, 崇; 古林, 与志安; 松本, 高太郎; 猪熊, 壽; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4102

ホルスタイン育成牛にみられた上衣芽腫の1 例
Provedor de dados: 19
Autores: 田川, 道人; 谷, 夏深; 梶原, 綾乃; 古林, 与志安; 古岡, 秀文; 松井, 高峯; 石井, 三都夫; 猪熊, 壽; TAGAWA, Michihito; TANI, Natsumi; KAJIWARA, Ayano; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; MATSUMOTO, Kohtaro; ISHII, Mitsuo; INOKUMA, Hisashi.
10 カ月齢のホルスタイン種雌育成牛が急性に斜頸，旋回，後弓反張，意識混濁の症状を呈した．大脳皮質壊死症を疑 い，ビタミンB1 製剤，コルチコステロイドおよび抗生物質による治療を実施したが反応せず，予後不良と判定された． 病理解剖により小脳に直径35. cm 大の腫瘤が確認され，左右側脳室および中脳水道の拡張がみられた．本症例は小脳腫 瘍による周辺部の圧迫および腫瘍の二次的圧迫による脳脊髄液流出障害により発生した内水頭症により，小脳徴候だけ でなく大脳および脳幹症状も出現したものと考えられた．病理組織学的検索の結果，本症例は上衣芽腫と診断された． A 10-month-old female Holstein heifer showed a sudden onset of clinical symptoms of head tilt，circling， increased extensor tone of all legs. and decreased level of consciousness. Cerebrocortical necrosis was suspected， and an administration of vitamin B1， corticosteroids，and anti-biotic was performed without a successful response. At necropsy，a neoplastic mass with a diameter of 3.5 cm in the cerebellum，dilated lateral ventricles on both sides and enlargement of the aqueducts cerebra...
Palavras-chave: 牛; 上衣芽腫; 神経症状; Cattle; Ependymoblastoma; Neurological signs.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2807

The Phylogenetic Position of Anaplasma bovis and Inferences on the Phylogeny of the Genus Anaplasma
Provedor de dados: 19
Autores: Adrian Patalinghug YBAÑEZ; SASHIKA, Mariko; INOKUMA, Hisashi; 猪熊, 壽.
The present study aimed to determine the complete citrate synthase (gltA) and heat-shock protein (groEL) gene sequences of Anaplasma bovis and to infer phylogenetic relationships within the genus Anaplasma. Multiple alignments from single and concatenated sequences of the 16S rRNA, gltA and groEL genes of the genus Anaplasma were subjected to phylogenetic analyses. Percent identities of A. bovis nucleotide sequences were found highest with A. phagocytophilum in gltA (65.4%) and groEL (79.8%). Single gene phylogenetic tree results assumed similar phylogenetic positions within the genus Anaplasma, except for A. bovis. However, consensus and concatenated sequence phylogenetic trees showed similar results, revealing 2 subgroups within the genus.
Palavras-chave: 16S rRNA gene; Anaplasma bovis; Citrate synthase gene (gltA); Heat-shock operon gene (groEL); Phylogeny.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3895

ホルスタイン種乳牛の鼻腔内骨肉腫の1例
Provedor de dados: 19
Autores: 吉本, 薫; 駒形, 真; 千葉, 史織; 弘, 雅信; 松本, 高太郎; 古林, 与志安; 猪熊, 壽; YOSHIMOTO, Kaoru; KOMAGATA, Makoto; CHIBA, Shiori; HIRO, Masahiro; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; INOKUMA, Hisashi.
5歳6カ月齢のホルスタイン種乳牛が鼻出血，眼球突出および黒色便を呈したため，地方病性牛白血病を疑った．し かし，生前の臨床検査では牛白血病の確定診断はできず，病理組織学的検査により鼻腔内骨肉腫と診断された．本症例では，明瞭な呼吸器症状や顔面の変形といった鼻腔内腫瘍を疑わせる所見が乏しく，生前診断が困難であった． Enzootic bovine leukosis was suspected in a Holstein cow aged 5-years and 6-months, as it displayed symptoms of epistaxis, proptosis, and melena. However, clinicopathological findings failed to confirm a diagnosis of bovine leukosis, and histopathological findings revealed a nasal osteosarcoma. As the case lacked typical findings of nasal tumor such as sneezing and facial deformity, a nasal tumor diagnosis would have been difficult.
Palavras-chave: ホルスタイン; 鼻腔内腫瘍; 骨肉腫; Holstein; Nasal tumor; Osteosarcoma.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3554

牛ウイルス性下痢ウイルス感染に関連するミエリン形成不全により先天性小脳症状を呈したホルスタイン種子牛の５症例
Provedor de dados: 19
Autores: 猪熊, 壽; 小嶋, 由夏; 千葉, 史織; 古林, 与志安; 高垣, 勝仁; INOKUMA, Hisashi; KOBAYASHI, Yoshiyasu.
2011年12月〜2012年３月に十勝管内の１牧場から神経症状を呈するホルスタイン種新生子牛５頭が帯広畜産大学に搬入された．いずれの症例も振戦，起立不能ないし難渋，開脚姿勢，歩様蹌踉，企図振戦などの神経症状を呈しており，生前には小脳病変が疑われた．血液および生化学検査では低ガンマグロブリン血症および低コレステロール血症が認められた他は，顕著な異常はみられなかった．安楽殺後または死亡後の病理解剖では，いずれの症例においても白質硬化がみられたが，小脳低形成などの肉眼的形態異常は認められなかった．病理組織学的検索では，広範な中枢神経領域の白質におけるミエリン形成不全とアストログリアの増殖が共通所見として認められた．血液を材料としたRT-PCR検査により牛ウイルス性下痢ウイルス（BVDV）を検索したところ，すべての症例からBVDV-1型遺伝子が検出され，２症例からはBVDVウイルスも分離された．本症例群でみられた先天性小脳症状は，胎子期のBVDV感染の症状としては稀なミエリン形成不全により生じた可能性が高いと考えられた．
Palavras-chave: 牛ウイルス性下痢ウイルス（BVDV）; 小脳症状; 白質硬化; ミエリン形成不全.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3892

片側性胸水を伴う血様心囊水貯留の乳牛の1症例
Provedor de dados: 19
Autores: 山川, 和宏; 杉崎, 義一; 吉林台; 古林, 与志安; 古岡, 秀文; 佐々木, 直樹; 石井, 三都夫; 猪熊, 壽; YAMAKAWA, Kazuhiro; SUGISAKI, Giichi; Jilintai; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; SASAKI, Naoki; ISHII, Mitsuo; INOKUMA, Hisashi.
2歳5カ月齢ホルスタイン雌乳牛が胸垂冷性浮腫，頸静脈拍動を呈し，心音聴取困難および心電図各波低電位所見から拡張型心筋症が疑われた．右胸腔には赤色変性性漏出液が多量に貯留しており，また超音波検査により心嚢水貯留を認めた．病理解剖の結果，心嚢は直径60cm大に腫大して血様心嚢水の貯留を認めたが，炎症あるいは腫瘍は認められず，血様心嚢水貯留の原因については不明であった．ただし右肩部に壊死巣を認めたことから，転倒等胸腔に強い衝撃を受けた可能性があると考えられた．心嚢水貯留が認められる場合には，心嚢腔内への出血も考慮すべき疾病のひとつであることが考えられた． A Holstein cow (two years and five months old) with jugular vein distension and non-inflammatory edema in the brisket showed decreased heart sound and abnormal waves on an electrocardiogram. Dilated cardiomyopathy was suspected. Unilateral pleural effusion was recovered from the right pleural cavity and found to be degenerated transudations. An ultrasonic cardiogram detected pericardial fluid. A pathological examination revealed that the pericardial cavity was enlarged with a...
Palavras-chave: 牛; 血様心嚢水; 片側性胸水貯留; Cow; Blood-like pericardium; Unilateral pleural efftusion.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2804

A PCR Based Survey of Babesia ovata in Cattle from Various Asian, African and South American Countries
Provedor de dados: 19
Autores: SIVAKUMAR, Thillaiampalam; ASADA, Masahito; BATTSETSEG, Badgar; HUANG, Xiaohong; LAN, Dinh Thi Bich; INPANKAEW, Tawin; P. YBAÑEZ, Adrian; ALHASSAN, Andy; THEKISOE, Oriel M. M.; DE MACEDO, Alan Caine Costa; INOKUMA, Hisashi; IGARASHI, Ikuo; YOKOYAMA, Naoaki; 猪熊, 壽; 五十嵐, 郁男; 横山, 直明.
Babesia ovata is a tick-transmitted hemoprotozoan parasite that infects cattle. In our study, bovine blood samples (n=2,034) were collected from 10 different countries (Brazil, China, Ghana, Japan, Mongolia, the Philippines, South Africa, Sri Lanka, Thailand and Vietnam) and DNA extracted. The DNA samples were screened using an established and specific polymerase chain reaction (PCR) assay targeting the Apical membrane antigen 1 (AMA-1) gene. Parasite DNA was detected among samples collected from Japan, Mongolia and Thailand. Sequence analyses confirmed that the PCR assay detected only B. ovata AMA-1, and that amplicons from different geographical locations were conserved. Our findings highlight the importance of designing adequate strategies to control B....
Palavras-chave: AMA-1; Babesia ovata; Cattle; Mongolia; Thailand.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4016

起立不能を呈した育成牛における非定型散発性牛白血病の1例
Provedor de dados: 19
Autores: 松山, 雄喜; 村上, 智亮; 大林, 哲; 古林, 与志安; 松本, 高太郎; 松井, 高峯; 猪熊, 壽; MATSUYAMA, Yuuki; MURAKAMI, Tomoaki; OHBAYASHI, Tetsu; KOBAYASHI, Yoshiyasu; MATSUMOTO, Kotaro; MATSUI, Takane; INOKUMA, Hisashi.
8カ月齢ホルスタイン種乳牛が起立不能を呈した.右下顎リンパ節相当部に腫瘤を認めたため，午白血病を疑い血液検査および針吸引細胞診を実施したが，確定診断に至らなかった.病理解剖においては，環椎および胸椎にも腫瘤が存在し脊髄を圧迫していた.病理組織学的に，腫瘍細胞はBLA-36抗原陽性であり，B細胞性リンパ腫と診断した. BLV抗体は陰性であった.これらのことから，本症例は非定型若齢牛の散発性牛白血病と考えられた. An 8-month-old Holstein heifer presented with difficulty in standing. Bovine leukosis was suspected because of the enlargement of the right mandibular lymph node; however，this could not be confirmed by fine needle aspiration of the lymph node and blood examination. A postmortem examination revealed that neoplastic masses compressed the spinal cord and nerves at the atlas and thoratic vertebra. A histopathological examination revealed the mass to be B-cell lymphoma based on BLA-36 positivity，but antibodies against bovine leukemia virus were not detected....
Palavras-chave: 非定型; 散発性牛白血病; 起立不能; Atypical form; Sporadic bovine leukosis; Astasia.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2943

心室腔拡張と心室壁菲薄化を伴わないホルスタイン種乳牛の心筋症の１症例
Provedor de dados: 19
Autores: 池川, 晃世; 新谷, 紗代; 松本, 高太郎; 古岡, 秀文; 木田, 克弥; 猪熊, 壽; MATSUMOTO, Kotaro; FURUOKA, Hidefumi; INOKUMA, Hisashi.
４歳２カ月齢のホルスタイン種乳牛が分娩２カ月後に、食欲不振、乳量減少を主訴に初診を受けた。初診時の 身体検査では、頸静脈の怒張・拍動が認められ、聴診ではギャロップ音が聴取された。心電図検査でP とT 波 の増高が認められたが、著しい低電位はみられなかった。心音図検査では 音が確認された。また、心臓超音 波検査では、肺動脈と右心房の拡張が認められたが、心室腔拡張、収縮率低下および心室壁菲薄化はみられな かった。血液検査では炎症像は認められず、生前の確定診断には至らなかった。病理解剖では、肉眼的に心筋の 褪色、両心室壁の軽度肥厚が認められたが、心室腔拡張と心室壁菲薄化はみられなかった。病理組織学的には 両心室壁および中隔で心筋線維の肥大・大小不同、空胞変性および間質の線維化所見から心筋症と診断された。
Ano: 2014
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4009

Calf Form Bovine Leukosis with Lameness in a Holstein Heifer
Provedor de dados: 19
Autores: TAWFEEQ, Mohammad Monir; MIURA, Saori; NAKANISHI, Yuuki; SUGIMOTO, Kazuya; KOBAYASHI, Yoshiyasu; FURUOKA, Hidefumi; INOKUMA, Hisashi; 古林, 与志安; 猪熊, 壽.
A 12-month-old Holstein heifer with anorexia, lameness, and enlargement of peripheral lymph nodes was suspected of having bovine leukosis. Although lymphocytosis was not observed, cytology of fine needle aspirate from a superficial cervical node, and increased serum lactate dehydrogenase and thymidine kinase activities, strongly suggested lymphosarcoma. Increased numbers of mononuclear cells as well as mitotic cells were observed in synovial fluid collected from swollen joints. Pathological examination confirmed B-cell calf form bovine leukosis and joint swelling related to neoplastic cell infiltration. Both interleukin-2 receptor and thymidine kinase 1 genes were highly expressed in cells from superficial cervical lymph node aspirate.
Palavras-chave: Calf form bovine leukosis; Gene expression; Joint infiltration.
Ano: 2012
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4097

Cerebellar Cortical Degeneration in a Japanese Black Bull
Provedor de dados: 19
Autores: 田川, 道人; 千葉, 史織; 岡松, 弘之; 小嶋, 由夏; 松本, 高太郎; 古林, 与志安; 猪熊, 壽; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
黒毛和種牛が出生翌日より横臥，後弓反張を呈した．脳挫傷を疑い，抗生物質，ビタミンB1製剤，消炎鎮痛剤により加療され，いったんは改善したが同様の症状が間欠的に認められた．歩様異常は継続して認められたが，一般状態は良好で8カ月齢まで飼養された．しかし，その後開脚姿勢，測定過大を伴う歩様異常，威嚇瞬き反応の低下が進行した．病理組織学的検索により小脳にプルキンエ細胞の顕著な変性と脱落及び顆粒細胞の減数を認め，小脳皮質変性症と診断された．
Palavras-chave: 牛; 小脳皮質変性症; 黒毛和種.
Ano: 2013
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4101

Genotypic Diversity of Theileria orientalis Detected from Cattle Grazing in Kumamoto and Okinawa Prefectures of Japan
Provedor de dados: 19
Autores: YOKOYAMA, Naoaki; UENO, Akio; MIZUNO, Daisuke; KUBOKI, Noritaka; KHUKHUU, Altangerel; IGARASHI, Ikuo; MIYAHARA, Tokuji; SHIRAISHI, Takashi; KUDO, Ryuta; OSHIRO, Mamoru; ZAKIMI, Satoshi; SUGIMOTO, Chihiro; MATSUMOTO, Kotaro; INOKUMA, Hisashi; 横山, 直明; 五十嵐, 郁男; 松本, 高太郎; 猪熊, 壽.
Theileria orientalis is a benign protozoan species that is widely distributed in Japan, yet sometimes causes serious economic losses in the livestock industry. In this study, we conducted a molecular survey based on genes encoding the major piroplasm surface protein (MPSP) and p23 for T. orientalis detected in cattle grazing in southern areas of Japan, consisting of 2 farms in Kumamoto prefecture (Aso and Kuma districts) and 3 farms in Okinawa prefecture (Ishigaki, Iriomote, and Yonaguni Islands). High prevalence rates of T. orientalis infection were shown in all the cattle populations using the diagnostic MPSP- and p23-PCR assays. Phylogenetic analyses revealed 4 MPSP genotypes and 3 p23 genotypes. Furthermore, MPSP genotype-specific PCR methods were...
Palavras-chave: Cattle; Genotype-specific PCR; Japan; MPSP gene; Theileria orientalis.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3584

重度雄性化を伴う褐毛和種フリーマーチン肥育牛にみられた腎後性腎不全の1例
Provedor de dados: 19
Autores: 吉本, 薫; 海老名, 千尋; 高橋, 英二; 古岡, 秀文; 松本, 高太郎; 松井, 基純; 三宅, 陽一; 猪熊, 壽; YOSHIMOTO, Kaoru; EBINA, Chihiro; TAKAHASHI, Eiji; FURUOKA, Hidefumi; MATSUMOTO, Kotaro; MATSUI, Motozumi; MIYAKE, Yo-ichi; INOKUMA, Hisashi.
排尿異常を認める21カ月齢の褐毛和種フリーマーチン肥育牛が，臨床的に水腎症および腎不全と診断された. 病理学的検査において，泌尿生殖器の重度雄性化，腎膿瘍および尿路結石が明らかとなった. 本症例は異常な尿道形態から尿路感染症と尿石症を継発し，最終的に腎後性腎不全に陥ったまれなフリーマーチン症例である. A Japanese Brown freemartin (21 months old) was clinically diagnosed with hydroneprosis and renal failure. A pathological examination revealed severe masculinization of the urogenital organs， pyonephritis and urolithiasis. These findings suggested that it was a rare case of a freemartin with a post-renal failure and morphological anomaly of the urinary tract，complicated by a bacterial infection and urolithiasis. 一一一Keywords :fr eemartin，po st-renal failure，se vere masculinization
Palavras-chave: フリーマーチン; 腎後性腎不全; 重度雄性化; Freemartin; Post-renal failure; Severe masculinization.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2944

帯広畜産大学 第3回公開総合臨床学実習(馬専攻・牛専攻)の概要
Provedor de dados: 19
Autores: 佐々木, 直樹; 山田, 一孝; 石井, 三都夫; 松井, 基純; 羽田, 真悟; 松本, 高太郎; 古岡, 秀文; 古林, 与志安; 猪熊, 壽; SASAKI, Naoki; YAMADA, Kazutaka; ISHII, Mitsuo; Matsui, Motozumi; HANEDA, Shingo; Matsumoto, Kotaro; FURUOKA, Hidefumi; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4378

ホルスタイン種搾乳牛にみられた心室中隔欠損の1症例
Provedor de dados: 19
Autores: 角田, 浩之; 中川, 大輔; 梶原, 綾乃; 松本, 高太郎; 古林, 与安志; 猪熊, 壽; MATSUMOTO, Kotaro; KOBAYASHI, Yoshiyasu; INOKUMA, Hisashi.
２歳５カ月齢のホルスタイン種搾乳牛が、分娩約３カ月半後に食欲不振を呈した。聴診により、左右胸壁から全収縮期雑音が聴取されたことから心内膜炎が疑われた。しかし、冷性浮腫や頸静脈怒張等のうっ血心不全症状はなく、また血液および血液生化学検査にて炎症像はみられなかった。心臓超音波検査により、心室中隔欠損、大動脈騎乗、右心室壁肥厚、大動脈および左右心室の拡張が認められた。病理解剖により、心臓超音波検査でみられた異常に加えて、後大静脈と肺動脈の著しい拡張が明らかとなった。心室中隔欠損を伴う牛が分娩後３カ月半経過してから症状を発現したまれな症例と思われた。
Palavras-chave: 心室中隔欠損; 搾乳牛; ホルスタイン種.
Ano: 2015
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4180