Consulta: creatorFacets:"Li,Zhongai"
Registros recuperados: 3
Data/hora: 03/06/2024 05:07:04

Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana
Provedor de dados: 69
Autores: Li,Zhongai; Du,Yaqiong; Wang,Zicheng.
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods. Results: The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis. The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and...
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Cryopreservation; Real-time PCR; Transformation efficiency.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600012

DNA demethylation during Chrysanthemum floral transition following short-day treatment
Provedor de dados: 69
Autores: Li,Zhongai; Li,Jie; Yanhua,Liu; Wang,Zicheng.
Background: Analytical techniques such as methylation-sensitive amplification polymorphism and high-performance liquid chromatography were used to detect variation in DNA methylation of mature Chrysanthemum leaves during the floral transition induced by short-day (SD) treatment. Results: For both early- and late-flowering cultivars, the time from the date of planting to the appearance of the capitulum bud and early blooming were significantly shorter than those of the control. The capitulum development of the early-flowering cultivar was significantly accelerated compared to the control, unlike the late-flowering cultivar. The DNA methylation percentage of leaves was significantly altered during flower development. For the early-flowering cultivar, DNA...
Tipo: Journal article
Palavras-chave: Chrysanthemum; DNA; Methylation; Floral transition; Short day.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000300009

Effect of nickel chloride on Arabidopsis genomic DNA and methylation of 18S rDNA
Provedor de dados: 69
Autores: Li,Zhongai; Chen,Xin; Li,Suoping; Wang,Zicheng.
Background In recent years, nickel (Ni) has been widely applied in industrial and agricultural production and has become a kind of environmental pollution. In this study, the effect of nickel chloride (NiCl2) with different concentrations on Arabidopsis genomic stability and DNA methylation has been demonstrated. The nucleolus variation and 18S rDNA methylation after NiCl2 treatment have been analyzed. Results The results are as follows: (1) The NiCl2 could result in heritable genomic methylation variations. The genomic DNA methylation variations have been detected by methylation-sensitive amplified polymorphism (MSAP) molecular markers, and the result showed that after NiCl2 treatment, there was methylation variation in T0 generation seedlings, and...
Tipo: Journal article
Palavras-chave: DNA methylation; Heavy metal; Methylation sensitive amplified polymorphism (MSAP); Nickel.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000100010