Consulta: creatorFacets:"Quoirin,Marguerite"
Registros recuperados: 12
Data/hora: 14/06/2024 21:20:03

Xyloglucans from Hymenaea courbaril var. stilbocarpa seeds affect Arabidopsis thaliana seedling growth by enhancing lateral root development
Provedor de dados: 88
Autores: Salamoni,Adriana Tourinho; Sierakowski,Maria-Rita; El Jaziri,Mondher; Quoirin,Marguerite.
The effect of crude xyloglucan (XG) preparations from jatobá (Hymenaea courbaril var. stilbocarpa (Hayne) Y. T. Lee &amp;amp; Langenh.) seeds on Arabidopsis thaliana (L.) Heynh. root system development was investigated. The XG extracts exerted a dual effect on root system development by slowing down root growth and improving lateral root formation. These observed morphological changes were not due to oligosaccharides that could be generated following hydrolysis of the XG polymers, since XG hydrolysate induced a drastic inhibition of the overall growth process of the Arabidopsis thaliana seedlings. Histochemical test of GUS gene expression assay performed on seven and 14-days-old transgenic Arabidopsis thaliana plants carrying the CycB1;1-GUS fusion...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jatobá; Rhizogenesis; Xyloglucan activity.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042010000400003

Produção de etileno e atividade da enzima ACCoxidase em frutos de maracujá-amarelo (Passiflora edulis f. flavicarpa Deg.)
Provedor de dados: 94
Autores: Winkler,Larissa Macedo; Quoirin,Marguerite; Ayub,Ricardo; Rombaldi,César; Silva,Jorge.
O maracujá-amarelo (Passiflora edulis f. flavicarpa Deg.) é uma das principais espécies cultivadas do gênero Passiflora. Seus frutos são ricos em minerais, vitaminas e apresentam aroma e sabor agradáveis. O presente trabalho teve como objetivos determinar a produção de etileno e atividade enzimática da ACCoxidase (ACCO) em maracujá-amarelo colhido em diferentes estádios de maturação. A determinação do estádio de maturação foi realizada com o auxílio de um colorímetro, que, por meio da radiação ultravioleta, estabeleceu valores absolutos da cor dos frutos de cada um dos grupos (I, II e III). A produção de etileno e a atividade da ACCoxidase foram realizadas por cromatografia gasosa. Os frutos do grupo I são predominantemente verdes, de acordo com os valores...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Maracujá-amarelo; Etileno; ACCO.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300014

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis
Provedor de dados: 63
Autores: Dibax,Roberson; Eisfeld,Cristiane de Loyola; Cuquel,Francine Lorena; Koehler,Henrique; Quoirin,Marguerite.
Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L-1 NAA and 4.44 µmol L-1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Forest species; Culture media; Organogenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400016

Organogenesis and transient genetic transformation of the hybrid Eucalyptus grandis × Eucalyptus urophylla
Provedor de dados: 63
Autores: Alcantara,Giovana Bomfim de; Bespalhok Filho,João Carlos; Quoirin,Marguerite.
The hybrid Eucalyptus grandis x Eucalyptus urophylla presents high levels of productivity and potential for use in paper, cellulose and fiber industries. The bud organogenesis from leaf explants of two clones of E. grandis × E. urophylla was studied in order to verify the effect of several factors: subculture duration on multiplication medium, type of explants, entire and half leaves: basal and apical portions, and duration of the culture on a regeneration medium. Differences in organogenic capacity of the two clones tested were observed. The explant most recommended for organogenesis is the basal section of the leaf collected from shoot clusters subcultured every 17 days. Moreover, the leaf explants must be transferred to a fresh bud induction medium...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Agrobacterium tumefaciens; Micropropagation; Tissue culture; Transgenic plant; Woody species.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000200016

Somatic embryogenesis in Acrocomia aculeata Jacq. (Lodd.) ex Mart using the thin cell layer technique
Provedor de dados: 39
Autores: Padilha,João Henrique Delfrate; Ribas,Luciana Lopes Fortes; Amano,Érika; Quoirin,Marguerite.
Considering the necessity of Acrocomia aculeata propagation for large-scale production, the aim of this study was to establish a somatic embryogenesis protocol using the thin cell layer (TCL) technique. Aerial parts of in vitro plants were transversally cut at the base into eight TCLs and placed in a culture medium for callus induction. The induction medium was composed of Y3 salts and Morel´s vitamins and supplemented with 150, 300 or 600 μM picloram. After 12 weeks the calli were transferred to a medium supplemented with BAP or 2-iP (12.5 or 25 μM). After 18 weeks, the somatic embryo clusters were transferred to a conversion medium (plant growth regulator-free medium). Primary callus induction rate was higher in the first three TCLs and in media...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2-iP; Callus induction; Histology; Macaw palm; Picloram.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000400516

Anatomia comparada das folhas e raízes de Cymbidium Hort. (Orchidaceae) cultivadas ex vitro e in vitro
Provedor de dados: 39
Autores: Mayer,Juliana Lischka Sampaio; Ribas,Luciana Lopes Fortes; Bona,Cleusa; Quoirin,Marguerite.
Na fase de cultivo in vitro, as plantas são mantidas em ambiente com alta umidade relativa do ar, baixa luminosidade e trocas gasosas restritas, o que resulta em taxa de transpiração reduzida. Portanto, quando essas mudas são expostas ao meio ex vitro, sofrem estresse que pode causar a morte. O objetivo desse trabalho foi comparar a estrutura anatômica das mudas de Cymbidium 'Joy Polis' cultivadas ex vitro (planta matriz e aclimatizada) e in vitro e verificar se a estrutura anatômica das plantas in vitro influencia no processo de aclimatização. As plantas ex vitro foram mantidas em casa-de-vegetação, em vasos individuais com o substrato fibra de coco em pó combinada com fibra de coco, e as plantas in vitro foram mantidas em meio de cultura MS. Para a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orquídea; Cultura in vitro; Morfologia; Aclimatização.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062008000200003

Induction of leafy galls in Acacia mearnsii De Wild seedlings infected by Rhodococcus fascians
Provedor de dados: 52
Autores: Quoirin,Marguerite; Bona,Cleusa; Souza,Elizandra F. de; Schwartsburd,Pedro B.
Plantlets of blackwattle (Acacia mearnsii De Wild) were inoculated with the bacterium Rhodococcus fascians and cultured in vitro. Leafy galls appeared at the cotyledonary nodes in 75% of the infected plants. The galls were separated from the plants and cultured on a medium containing three-quarters-strength MS salts (Murashige and Skoog, 1962), MS vitamins, 2% sucrose and an antibiotic (cephalothin), supplemented with or without 0.2% activated charcoal. Histological studies conducted from the sixth to the twenty-second day after plant infection revealed the presence of newly formed meristematic centers, first in the axillary region, then on the petioles and lamina of the leaflets around the apical meristem. Approximately 37% of the galls developed one...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Black wattle; Legume tree; Micropropagation.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132004000300002

Inheritance and linkage relationships of allozyme variants of ilex paraguariensis St. Hil
Provedor de dados: 52
Autores: Wendt,Simone Neumann; Sousa,Valderês Aparecida de; Sebbenn,Alexandre Magno; Sturion,José Alfredo; Luíz,Felipe; Santos,Elisa Caroline da Silva; Quoirin,Marguerite.
Eighteen enzyme systems were examined in Ilex paraguariensis St. Hil. using starch gel electrophoresis. Seven out of 12 active isozyme systems revealed one or more polymorphic loci (PGI, GOT, MR, G-6PDH, MDH, NDH, and 6-PGDH). However, the segregation and linkage analyses were performed only for PGI, GOT, G-6PDH and 6-PGDH systems. Gene segregation at these loci was regular, except for a few trees that showed segregation distortion. Weak linkage disequilibrium between loci was detected, but it was not enough to influence the multilocus estimate.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Erva-mate (matê); Electrophoresis; Loci; Allele; Segregation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600016

Selective agent and A. tumefaciens overgrowth-control antibiotics in Eucalyptus camaldulensis Cotiledonary culture
Provedor de dados: 52
Autores: Quisen,Regina; Oliveira,Yohana de; Pileggi,Marcos; Cuquel,Francine; Quoirin,Marguerite.
The objectives of the present work were to establish the minimal lethal dose of the selective agent to determine the type and concentration of appropriate antibiotics for the elimination of Agrobacterium tumefaciens inoculated explants, without interfering with the regenerative potential of the E. camaldulensis cotyledonary explants. Non-transformed explants were cultivated in medium supplemented with kanamycin. The results showed that the antibiotic was suitable for the selection of transformed cells in the concentration of 9 mg L-1 as it inhibited the growth of non-transformed cells. Cotyledons infected with A. tumefaciens were cultivated in MS N/2 medium supplemented with BAP, ANA, Km and cefotaxime or AugmentinÒ . The highest average of regenerated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Morphogenesis; Kanamycin; Cefotaxime; Augmentin; Agrobacterium tumefaciens; Eucalypt.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000600020

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis dehn and histological study of organogenesis in vitro
Provedor de dados: 52
Autores: Dibax,Roberson; Quisen,Regina Caetano; Bona,Cleusa; Quoirin,Marguerite.
The present work aimed at regenerating plants of Eucalyptus camaldulensis from the cotyledonary explants and describing the anatomy of the tissues during callogenesis and organogenesis processes, in order to determine the origin of the buds. The cotyledonary leaves of E. camaldulensis were cultured in Murashige and Skoog (MS), WPM and JADS media supplemented with 2.7 µM NAA and 4.44 µM BAP. The best results for bud regeneration were obtained on MS and WPM media (57.5 and 55% of calluses formed buds, respectively). Shoot elongation and rooting (80%) were obtained on MS/2 medium (with half-strength salt concentration) with 0.2% activated charcoal. Acclimatization was performed in the growth chamber for 48 h and then the plants were transferred to a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious buds; Callogenesis; Organogenesis; Tissue culture; Anatomical study.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000200009

MISTURAS VITAMÍNICAS NA REGENERAÇÃO DO MARACUJAZEIRO AMARELO (Passiflora edulis f. flavicarpa Deg.)
Provedor de dados: 65
Autores: Ribas,Alessandra Ferreira; Denis,Francine; Quoirin,Marguerite; Ayub,Ricardo Antonio.
Cotilédones obtidos a partir de sementes germinadas in vitro foram usados como explantes com o objetivo de avaliar o efeito de dois complexos vitamínicos e de duas concentrações de 6-benzilaminopurina (BAP) na organogênese direta do maracujá amarelo (Passiflora edulis f. flavicarpa Deg.). Empregou-se o meio Murashige &amp;amp; Skoog (1962) suplementado com as vitaminas MS ou B5 (vitaminas de GAMBORG et al., 1968) e 1 ou 2 mg.&lt;img SRC="http:/img/fbpe/cr/v32n2/a09img01.gif"&gt; -1 de BAP. Foram testados quatro tratamentos, sendo cada um constituído de 60 explantes. O experimento foi inteiramente casualizado, com dez repetições. Os dados, analisados pelo teste de Tukey, demonstraram que os meios de cultura suplementados com o complexo vitamínico B5...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Organogênese; Cultura de tecidos; Citologia; Passiflora edulis f. flavicarpa Deg.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782002000200009

Pré-aclimatização in Vitro de abacaxi-ornamental
Provedor de dados: 64
Autores: Oliveira,Yohana de; Anselmini,Justina Inês; Cuquel,Francine Lorena; Pinto,Fernanda; Quoirin,Marguerite.
O abacaxi ornamental (Ananas bracteatus Schult. f.) é bastante utilizado em composições paisagísticas para delimitar áreas ou canteiros. A micropropagação permite obter altas taxas de multiplicação, podendo ser uma alternativa viável e vantajosa de propagação vegetativa, visto que a demanda por esta espécie vem aumentando, tanto no mercado nacional quanto no internacional. Neste trabalho objetivou-se avaliar a influência de diferentes irradiâncias e concentrações de sacarose durante o enraizamento in vitro, sobre a sobrevivência das mudas na fase de aclimatização. Para tanto, foram testadas diferentes concentrações de sacarose (0, 10, 20 e 30 g L-1) no meio de enraizamento, bem como duas irradiâncias (22,9 e 66,9 mmol m-2 s-1) em sala de crescimento. O...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas bracteatus; Micropropagação; Planta ornamental.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542010000700010