Consulta: creatorFacets:"Suzuki, Hiroshi"
Registros recuperados: 17
Data/hora: 10/06/2024 13:52:36

Emergence of multi-acaricide resistant Rhipicephalus ticks and its implication on chemical tick control in Uganda
Provedor de dados: 19
Autores: Vudriko, Patric; Okwee-Acai, James; Tayebwa, Dickson Stuart; Byaruhanga, Joseph; Kakooza, Steven; Wampande, Edward; Omara, Robert; Muhindo, Jeanne Bukeka; Tweyongyere, Robert; Owiny, David Okello; Hatta, Takeshi; Tsuji, Naotoshi; Umemiya-Shirafuji, Rika; Xuan, Xuenan; Kanameda, Masaharu; Fujisaki, Kozo; Suzuki, Hiroshi.
Background: Acaricide failure has been on the rise in the western and central cattle corridor of Uganda. In this study, we identified the tick species associated with acaricide failure and determined their susceptibility to various acaricide molecules used for tick control in Uganda. Methods: In this cross sectional study, tick samples were collected and identified to species level from 54 purposively selected farms (from 17 districts) that mostly had a history of acaricide failure. Larval packet test was used to screen 31 tick populations from 30 farms for susceptibility at discriminating dose (DD) and 2 x DD of five panels of commercial acaricide molecules belonging to the following classes; amidine, synthetic pyrethroid (SP), organophosphate (OP) and...
Palavras-chave: Ticks; Rhipicephalus appendiculatus; Rhipicephalus (Boophilus) decoloratus; Acaricide; Resistance; Amitraz; Synthetic pyrethroids; Organophosphates.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4437

The epidemiological survey for atovaquone resistant related gene of Babesia gibsoni in Japan
Provedor de dados: 19
Autores: Iguchi, Aiko; Soma, Takehisa; Suzuki, Hiroshi; Xuan, Xuenan.
In 73 gDNA samples from Babesia gibsoni-infected dogs, the M121I variant population was measured by using allele-specific real-time PCR. Although the mechanism of atovaquone against B. gibsoni has not been clearly identified, it is reported that the mitochondria cytochrome b gene of the atovaquone-resistant B. gibsoni had a single-nucleotide substitution at nt363 (G to T), which resulted in the substitution of methionine with isoleucine (M121I). In this study, 3/73 samples showed over 5% M121I variant population. Although the M121I variant population is a low percentage, it runs the risk of spreading drug-resistant parasites. It is important to prevent the spread of drug-resistance, so we need to gather information about this at regular intervals.
Palavras-chave: Allele-specific real-time PCR; Babesia gibsoni; Drug resistance; M121I variant population.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4421

Enzyme-linked immunosorbent assays using recombinant TgSAG2 and NcSAG1 to detect Toxoplasma gondii and Neospora caninum-specific antibodies in domestic animals in Turkey
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Sevinc, Ferda; Sevinc, Mutlu; Ceylan, Onur; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Suzuki, Hiroshi; Nishikawa, Yoshifumi; Xuan, Xuenan.
Considering the scarce information on occurrences of Toxoplasma gondii and Neospora caninum in domestic animals from Turkey, the aim of this study was to investigate the seroprevalence of these parasite infections in cattle, horses, sheep, goats and dogs in Turkey. The specific antibodies against T gondii and N. caninum were detected by iELISAs based on the recombinant TgSAG2 or NcSAG1 in a total of 2,039 serum samples from eleven provinces. The seroprevalence of T. gondii infections was 46.3%, 4.0%, 20.0%, 12.9% and 19.8%, that of N. caninum infections was 0.3%, 7.4%, 2.1%, 3.2% and 16.6% in the horses, cattle, sheep, goats and dogs, respectively. These results indicated that T gondii and N. caninum infections are prevalent in Turkish domestic animals.
Palavras-chave: ELISA; Neospora caninum; Toxoplasma gondii; Turkey.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4422

TRANSIENT EXPRESSION OF A GREEN FLUORESCENT PROTEIN GENE IN BABESIA BOVIS
Provedor de dados: 19
Autores: Fujii, Kei; Yokoyama, Naoaki; Takabatake, Noriyuki; Suzuki, Hiroshi; Xuan, Xuenan; Igarashi, Ikuo; 横山, 直明; 鈴木, 宏志; 玄, 学南; 五十嵐, 郁男.
In hemoprotozoa，gene transfer technology provides an important tool to aid in the functional study of parasite genes. In this study，a transfer vector containing the enhanced green fluorescent protein (EGFP) gene laid between the Toxoplasma gondii GRAI promoter region and the GRA2 polyA signal region was constructed and transfected into the in vitro culture of Babesia boνis by the electroporation method.On the first and second days post-transfection，clear positive fluorescences were detected in some parasite bodies， indicating the successful expression of the EGFP gene controlled by the GRA-1 promoter in B. bovis. However， the positive parasites were not ubiquitous and finally disappeared until the forth day post-transfection. This is the first time that...
Ano: 2003
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/631

Prime-boost immunization with DNA followed by a recombinant vaccinia virus expressing P50 induced protective immunity against Babesia gibsoni infection in dogs.
Provedor de dados: 19
Autores: Fukumoto, Shinya; Tamaki, Yoh; Okamura, Masashi; Bannai, Hiroshi; Yokoyama, Natsuko; Suzuki, Tomoko; Igarashi, Ikuo; Suzuki, Hiroshi; Xuan, Xuenan.
A heterologous prime-boost immunization regime with priming DNA followed by recombinant vaccinia virus expressing relevant antigens has been shown to induce effective immune responses against several infectious pathogens. In this study, we constructed a recombinant plasmid and vaccinia virus, both of which expressed P50 of Babesia gibsoni, to investigate the immunogenicity and protective efficacy of a heterologous prime-boost immunization against canine babesiosis. The dogs immunized with the prime-boost regime developed a significantly high level of specific antibody against P50 when compared with the control groups, and the antibody level was strongly increased after a booster immunization with a recombinant vaccinia virus. The prime-boost immunization...
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/812

Identification of a novel gene encoding a secreted antigen 1 of Babesia gibsoni and evaluation of its use in serodiagnosis
Provedor de dados: 19
Autores: Jia, Honglin; Zhou, Jinlin; Ikadai, Hiromi; Matsuu, Aya; Suzuki, Hiroshi; Fujisaki, Kozo; Xuan, Xuenan; 鈴木, 宏志; 五十嵐, 郁男; 玄, 学南.
Serum from a dog immunized with blood plasma from a B. gibsoni-infected dog, putatively containing secreted antigens, was used to screen a cDNA expression library. A novel gene encoding BgSA1 was identified from the isolated clones. The serum raised in mice immunized with the recombinant BgSA1 expressed in Escherichia coli could recognize a native parasite protein with a molecular mass of 59 kDa. Comparing with the previously established ELISA with recombinant P50 as antigen, the ELISA with recombinant BgSA1 as the antigen was more sensitive when they were used to detect field samples. Moreover, a sandwich ELISA with anti-BgSA1 antibodies could detect the circulating BgSA1 in a serial blood plasma from a dog experimentally infected with B. gibsoni. These...
Palavras-chave: Babesia gibsoni; Secreted Antigen 1; Identification; Serodiagnosis.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1655

Incidence of apoptotic cells after vitrification in canine ovarian tissues
Provedor de dados: 19
Autores: Hariya, Madoka; Suzuki, Hiroshi.
Although vitrification and follicular survival subsequent to xenotransplantation of canine ovarian tissues have been reported, the degree of cryoinjury after the vitrification has not yet been fully examined. Since cryoinjury after vitrification of ovaries has been reported in bovine and humans, this study evaluated canine ovarian tissues after vitrification by immunohistochemistry and TUNEL assay. Ovaries were collected from immature bitches. The ovarian tissue cubes were pretreated with 1 M DMSO. Subsequently, DAP 213 solution (2 M DMSO, 1 M acetamide, 3 M propylene glycol) was added. The cryotubes containing the ovarian tissues were placed on ice for 5 min, then plunged directly into liquid nitrogen. After warming with 0.25 M sucrose, cryopreserved...
Palavras-chave: Cryopreservation; Dog; Follicle; Ovary.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4535

CLONING AND EXPRESSION OF AN ANTIGEN OF BABESIA GIBSONI IN ESCHERICHIA COLI AND ITS USE FOR THE IMMUNODIAGNOSIS OF CANINE BABESIOSIS
Provedor de dados: 19
Autores: Fukumoto, Shinya; Sekine, Yukiko; Kimbita, Elikira; Huang, Xiaohong; Xuan, Xuenan; Inoue, Noboru; Yokoyama, Naoaki; Igarashi, Ikuo; Fujisaki, Kozo; Sugimoto, Chihiro; Nagasawa, Hideyuki; Mikami, Takeshi; Suzuki, Hiroshi; 福本, 晋也; 玄, 学南; 井上, 昇; 横山, 直明; 五十嵐, 郁男; 鈴木, 宏志.
Palavras-chave: Babesia gibsoni; Babesia canis; CDNA library; ELISA; P30 gene.
Ano: 2002
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/624

動物の原虫感染に対する抵抗性遺伝子の発生工学的研究
Provedor de dados: 19
Autores: 鈴木, 宏志; Suzuki, Hiroshi.
発行年は推定
Ano: 2007
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3505

Molecular identification and antigenic characterization of a merozoite surface antigen and a secreted antigen of Babesia canis (BcMSA1 and BcSA1)
Provedor de dados: 19
Autores: Zhou, Mo; Cao, Shinuo; Luo, Yuzi; Liu, Mingming; Wang, Guanbo; Moumouni, Paul Franck Adjou; Jirapattharasate, Charoonluk; Iguchi, Aiko; Vudriko, Patrick; Terkawi, Mohamad Alaa; Lowenstein, Mario; Kern, Angela; Nishikawa, Yoshifumi; Suzuki, Hiroshi; Igarashi, Ikuo; Xuan, Xuenan.
Background: Babesia canis is an apicomplexan tick-transmitted hemoprotozoan responsible for causing canine babesiosis in Europe and west Asia. Despite its importance, there is no known rapid diagnostic kit detection of B. canis infection in dogs. The present study identified two novel antigens of B. canis and used the recombinant antigens to establish a rapid, specific and sensitive serodiagnostic technique for detection of B. canis infection. Methods: A complementary DNA (cDNA) expression library was constructed from the mRNA of B. canis and immunoscreened using B. canis-infected dog sera. The cDNAs encoding a merozoite surface antigen and a secreted antigen protein were identified and designated as BcMSA1 and BcSA1, respectively. The recombinant BcMSA1...
Palavras-chave: Babesia canis; Canine babesiosis; BcMSA1; BcSA1; ELISA; Immunochromatographic tests.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4420

Effect of Intraperitoneal Administration of Desialylated Erythropoietin on the Follicular Survival in Cryopreserved Canine Ovaries after Xenotransplantation
Provedor de dados: 19
Autores: Hirayama, Yukari; Inoue, Kou-suke; Suzuki, Hiroshi; 鈴木, 宏志.
The effect of intraperitoneal administration of desialylated erythropoietin (asialo EPO) on the follicular reserve of cryopreserved canine ovarian tissues was examined using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed under the dorsal skin of mice, and were then treated with saline or 400 or 4,000 U/kg/day of asialo EPO by intraperitoneal injection from one day before transplantation for 5 days. Transplanted tissues were removed and subjected to a histological examination 4 weeks after the transplantation. Without asialo EPO treatment, the mean number of primordial follicles in the cryopreserved ovarian tissues had decreased from 4.3/mm2 to 0.2/mm2 at 4 weeks after...
Palavras-chave: Canine; Cryopreservation; Ovary; Transplantation.
Ano: 2011
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3856

アズキ各部位から誘導した培養細胞の脂質の化学的特性
Provedor de dados: 19
Autores: 小嶋, 道之; 鈴木, 裕志; 大西, 正男; 伊藤, 精亮; Kojima, Michiyuki; Suzuki, Hiroshi; Ohnishi, Masao; Ito, Seisuke.
Palavras-chave: 培養細胞; グリセロ脂質; スフィンゴ脂質; 分子種; アズキ.
Ano: 1990
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/1628

The Role of Scavenger Receptor Type A (SR-A) during Infection with Babesia microti
Provedor de dados: 19
Autores: Kamada, Takenori; Igarashi, Ikuo; Inoue, Noboru; Nagasawa, Hideyuki; Suzuki, Hiroshi; Kodama, Tatsuhiko; Suzuki, Naoyoshi; Toyoda, Yutaka; 五十嵐, 郁男; 井上, 昇; 長澤, 秀行; 鈴木, 宏志.
Palavras-chave: Babesiosis; Protective immune response; Knock-out mouse; SRKO.
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/265

Probucol dramatically enhances dihydroartemisinin effect in murine malaria
Provedor de dados: 19
Autores: Kume, Aiko; Dang Trinh Minh Anh; Shichiri, Mototada; Ishida, Noriko; Suzuki, Hiroshi.
Background: Artemisinin-based combination therapy (ACT) has been adopted as national policy for the first-line treatment in large number of malaria-endemic regions. However, artemisinin-resistant parasites have emerged and are spreading, with slow-cleaning parasites being reported in patients treated with ACT. It means that more parasites are exposed to the partner drug alone and the risk of developing resistant parasites against the partner drug is increasing. Therefore, the development of a new method to enhance the effect of artemisinin is required. In this study, the potential effect of probucol as a combination drug of dihydroartemisinin (DHA), an artemisinin derivative, was examined. Methods: C57BL/6 J mice infected with Plasmodium yoelii XL-17 were...
Palavras-chave: C57BL/6 J mice; Dihydroartemisinin; Malaria; Oxidative stress; Probucol; Plasmodium yoelii XL-17; Vitamin E.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4436

Identification and characterization of cross-reactive antigens from Neospora caninum and Toxoplasma gondii
Provedor de dados: 19
Autores: Liao, Min; Xuan, Xuenan; Huang, Xiaohong; Shirafuji, Hiroaki; Fukumoto, Shinya; Hirata, Haruyuki; Suzuki, Hiroshi; Fujisaki, Kozo.
Murine monoclonal antibodies (mAbs) against Neospora caninum tachyzoites were produced to identify the cross-reactive antigens between N. caninum and Toxoplasma gondii. Ten mAbs recognizing cross-reactive antigens of both parasites were obtained and tentatively classified into 6 different groups based on their reactivity patterns in an indirect fluorescent antibody test and Western blot analysis. Three mAbs in group I recognized antigens located on the surface of parasites with molecular masses ranging from 28 to 76 kDa; one mAb in group 2 recognized antigens located on interior organelles of parasites with a molecular mass of 50 kDa; one mAb in group 3 recognized antigens located on interior organelles of parasites with molecular masses of 35 kDa and 14...
Palavras-chave: Neospora caninum; Toxoplasma gondii; Cross-reactive antigen; Protein disulfide isomerase; Heat-shock protein; Ribosomal protein 1.
Ano: 2005
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/816

Follicular Loss of the Cryopreserved Canine Ovary after Xenotransplantation
Provedor de dados: 19
Autores: Ishijima, Takako; Abe, Yasuyuki; Suzuki, Hiroshi; 鈴木, 宏志.
The effect of cryopreservation and subsequent xenotransplantation on the follicular reserve of the canine ovary by using non obese diabetic-severe combined immunodeficient (NOD-SCID) mice was examined. Vitrified-warmed canine ovarian tissues were placed into the ovarian bursa of mice, and then were removed and subjected to histological examination at 4 weeks after the transplantation. Over 30% of primordial follicles and 65% of early primary follicles survived after cryopreservation. However, regardless of breed or age, percentages of survived primordial follicles and early primary follicles after the transplantation ranged from 0-7% and from 0-15%, respectively. These results indicate that the majority of primordial follicles and early primary follicles...
Palavras-chave: Canine; Cryopreservation; Ovary; Transplantation.
Ano: 2009
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2802

優良盲導犬の育成に関する発生･生殖工学的研究 : イヌの性格はDNAに刻まれているか？
Provedor de dados: 19
Autores: 鈴木, 宏志; Suzuki, Hiroshi.
Palavras-chave: イヌ; 遺伝子　; 胚.
Ano: 2010
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2611