Consulta: subjectFacets:"Arabidopsis thaliana"
Registros recuperados: 14
Data/hora: 10/06/2024 06:35:41

Effect of cryopreservation on the efficiency of exogenous gene, genetic transformation and expression level of Arabidopsis thaliana
Provedor de dados: 69
Autores: Li,Zhongai; Du,Yaqiong; Wang,Zicheng.
Background: Cryopreservation refers to the storage of a living organism at ultra-low-temperature for long-term preservation of plant germplasm. The effect of cryopreservation on the efficiency of exogenous gene genetic transformation and expression level were studied herein. In this work, transgenic Arabidopsis thaliana were successfully conserved in vitro by cryopreservation methods. Results: The effects of osmotic stress due to cryoprotectants during pretreatment and of storage at -196ºC on the stability, the efficiency of genetic transformation and the expression level of exogenous gene were analyzed in Arabidopsis. The results showed that there had not any significant increasing in the efficiency of genetic transformation after cryopreservation, and...
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Cryopreservation; Real-time PCR; Transformation efficiency.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600012

Network analysis of ABA-dependent and ABA-independent drought responsive genes in Arabidopsis thaliana
Provedor de dados: 74
Autores: Liu,Shiwei; Lv,Zongyou; Liu,Yihui; Li,Ling; Zhang,Lida.
Abstract Drought is one of the most severe abiotic factors restricting plant growth and yield. Numerous genes functioning in drought response are regulated by abscisic acid (ABA) dependent and independent pathways, but knowledge of interplay between the two pathways is still limited. Here, we integrated transcriptome sequencing and network analyses to explore interplays between ABA-dependent and ABA-independent pathways responding to drought stress in Arabidopsis thaliana. We identified 211 ABA-dependent differentially expressed genes (DEGs) and 1,118 ABA-independent DEGs under drought stress. Functional analysis showed that ABA-dependent DEGs were significantly enriched in expected biological processes in response to water deprivation and ABA stimulus,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drought stress; Abscisic acid; RNA-seq; Gene expression; Protein-protein interaction network; Arabidopsis thaliana.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000400624

Mutations in retrotransposon AtCOPIA4 compromises resistance to Hyaloperonospora parasitica in Arabidopsis thaliana
Provedor de dados: 74
Autores: Wang,Yi-Hong; Warren Jr.,James T.
Retrotransposons (RTEs) are a principal component of most eukaryotic genomes, representing 50%-80% of some grass genomes. RTE sequences have been shown to be preferentially present in disease resistance gene clusters in plants. Arabidopsis thaliana has over 1,600 annotated RTE sequences and 56 of these appear to be expressed because of the exact expressed sequence tag (EST) matches and the presence of intact open reading frames. Of the 22 represented in the Affymetrix ATH1 array, AtCOPIA4 was found to be expressed at a higher level than all other RTEs across different developmental stages. Since AtCOPIA4 is located in the RPP5 gene cluster and is adjacent to RPP4 which confers resistance to the downy mildew oomycete Hyaloperonospora parasitica isolate...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis thaliana; Retrotransposon; Downy mildew resistance; Knockout.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100023

The impact of mechanical compression on cortical microtubules in Arabidopsis: a quantitative pipeline
Provedor de dados: 5
Autores: Louveaux, Marion; Rochette, Sebastien; Beauzamy, Lena; Boudaoud, Arezki; Hamant, Olivier.
Exogenous mechanical perturbations on living tissues are commonly used to investigate whether cell effectors can respond to mechanical cues. However, in most of these experiments, the applied mechanical stress and/or the biological response are described only qualitatively. We developed a quantitative pipeline based on microindentation and image analysis to investigate the impact of a controlled and prolonged compression on microtubule behaviour in the Arabidopsis shoot apical meristem, using microtubule fluorescent marker lines. We found that a compressive stress, in the order of magnitude of turgor pressure, induced apparent microtubule bundling. Importantly, that response could be reversed several hours after the release of compression. Next, we tested...
Tipo: Text
Palavras-chave: Mechanical stress; Indenter; Microtubules; Compression; Bundling; Arabidopsis thaliana; Technical advance.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00347/45829/45479.tiff

Flowering-time genes modulate meristem determinacy and growth form in Arabidopsis thaliana
Provedor de dados: 16
Autores: Melzer, S.; Lens, F.; Gennen, J.; Vanneste, S.; Rohde , A.; Beeckman, T.
Plants have evolved annual and perennial life forms as alternative strategies to adapt reproduction and survival to environmental constraints. In isolated situations, such as islands, woody perennials have evolved repeatedly from annual ancestors1. Although the molecular basis of the rapid evolution of insular woodiness is unknown, the molecular difference between perennials and annuals might be rather small, and a change between these life strategies might not require major genetic innovations2,3. Developmental regulators can strongly affect evolutionary variation4 and genes involved in meristem transitions are good candidates for a switch in growth habit. We found that the MADS box proteins SUPPRESSOR OF OVEREXPRESSION OF CONSTANS 1 (SOC1) and FRUITFULL...
Tipo: Article / Letter to the editor
Palavras-chave: Flowering-time; Meristem determinacy; Growth form; Arabidopsis thaliana; Insular woodiness; Secondary growth; Annual life forms; 42.56.
Ano: 2008
URL: http://www.repository.naturalis.nl/record/429531

Stem anatomy supports Arabidopsis thaliana as a model for insular woodiness
Provedor de dados: 16
Autores: Lens, F.; Smets, E.; Melzer, S.
The soc1 ful double mutant of A. thaliana produced substantial secondary growth throughout all aboveground stems, whereas in the Col accession only a few cell layers of wood were produced at the base of old stems. This increased wood formation may be linked to inactivation of the flowering time genes SOC1 and FUL (Melzer et al., 2008). These two genes could play a crucial regulatory role in triggering genetic mechanisms that lead to insular woodiness outside A. thaliana, and this ‘simple’ two-gene loss could explain why many nonrelated herbaceous families have independently evolved into woody insular lineages throughout the world. However, SOC1 and FUL might not be the only suppressors of cambium formation; other upstream, downstream or parallel-acting...
Tipo: Article / Letter to the editor
Palavras-chave: Stem anatomy; Arabidopsis thaliana; Insular woodiness; Brassicaceae; Wood formation; 42.56.
Ano: 2012
URL: http://www.repository.naturalis.nl/record/429528

Soybean cultivar BR-16-AHAS tolerance to the herbicide imazapyr
Provedor de dados: 86
Autores: Kiihl,Tammy Aparecida Manabe; Arias,Carlos Alberto Arrabal.
The objective of this work was to evaluate the effect of the transgenic soybean BR-16-AHAS genetic constitution on the tolerance to the herbicide imazapyr. BR-16-AHAS was crossed with ten other genotypes. The experimental design was a complete randomized block, in a 2x12x3 factorial arrangement, with two sowing periods (winter and summer), twelve crossing groups and three plant positions (upper, mid and lower), with three replicates. The plants were treated with 100 g ha-1 a.i. of imazapyr at the V3/V4 stage. For each position of the plant (upper, mid and lower), the following variables were assessed: number of pods, number of seeds, seed weight, number of seeds per pod and the 100 seeds weight. The effect of the herbicide varied according to the more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis thaliana; Glycine max; Epistasis; Genetic transformation.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2008000800012

Aplicación del análisis de rango reescalado R/S para la predicción de genes en el genoma vegetal
Provedor de dados: 109
Autores: Almanza Pinzón,Martha Isabel; López López,Karina; Téllez Villa,Carlos Eduardo.
La predicción de genes es en la actualidad uno de los principales desafíos de la genómica. La predicción permite realizar experimentos con alta probabilidad de encontrar genes de interés y comparar regiones de ADN de importancia agronómica entre genomas; además, ayuda a restringir los espacios de búsqueda en las bases de datos. Un procedimiento estadístico con base en el análisis R/S y el coeficiente de Hurst fue desarrollado para caracterizar y predecir genes y los componentes estructurales de estos (exones e intrones) en los genomas eucariotas completos de Arabidopsis thaliana, Oriza sativa y Mus musculus. Algoritmos en lenguaje de programación Python fueron desarrollados para extraer, filtrar y modelar más del 80% de las secuencias de genes registradas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genómica comparativa; Predicción de genes; Análisis R/S; Coeficiente de Hurst; Arabidopsis thaliana; Oryza sativa; Mus musculus.
Ano: 2010
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122010000400012

Evidencias empíricas de regularidades estadísticas y leyes de potencia en los genomas de Arabidopsis thaliana, Oriza sativa y Mus musculus
Provedor de dados: 109
Autores: Almanza P.,Martha I; López-López,Karina; Moreno,Pedro A; Téllez T.,Carlos E.
La masiva cantidad de datos biológicos provenientes de las disciplinas "ómicas" y su aprovechamiento en el mejoramiento genético vegetal requiere de nuevos abordajes teóricos y estadísticos que describan de forma satisfactoria principios generales en los genomas. El total de secuencias de los genes de los genomas vegetales de Arabidopsis thaliana y Oriza sativa y del genoma animal Mus musculus fueron extraídas y depuradas de la base de datos pública del Genebank mediante el diseño de algoritmos en lenguaje de programación Python. Se analizaron las distribuciones de las variables frecuencia de uso y tamaño de los genes, exones e intrones por cromosoma y entre genomas. Los resultados señalaron que las variables presentan patrones de comportamiento no...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genómica; Leyes de potencia; Arabidopsis thaliana; Oriza sativa; Mus musculus.
Ano: 2010
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122010000300002

Expression analysis of cell wall assembly and remodelling-related genes in Arabidopsis roots subjected to boron stress and brassinosteroid at different developmental stages
Provedor de dados: 39
Autores: İşkil,Rabia; Surgun-Acar,Yonca.
ABSTRACT Plant cell walls are affected by many biotic and abiotic stress conditions. The aim of this study is to determine the effects of 24-Epibrassinolide (EBL) on some cell wall-related genes in root tissue of five- and ten-week-old Arabidopsis thaliana plants exposed to boron (B) deficiency (0 µM) or toxicity (3000 µM) at the transcriptional level. Expressions of the genes that encode cellulose synthase (CESA1, CESA4, CESA6 and CESA8), cellulose synthase-like (CSLB5), expansin (EXPA5, EXPA8 and EXPA14) and cell wall protein (SEB1) decreased under conditions of B deficiency and toxicity. EBL treatments, in general, led the expressions of these genes to reduce significantly. Expressions of xyloglucan endotransglucosylase/hydrolase genes (XTH21 and XTH23)...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 24-Epibrassinolide; Arabidopsis thaliana; Boron; Cellulose synthase; Gene expression; Expansin; Pectin methylesterase; Xyloglucan endotransglucosylase/hydrolase.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062018000400546

Efeitos da radiação ultravioleta-B sobre a morfologia foliar de Arabidopsis thaliana (L.) Heynh. (Brassicaceae)
Provedor de dados: 39
Autores: Boeger,Maria Regina Torres; Poulson,Mary.
A redução da camada de ozônio resulta no aumento da radiação ultravioleta que atinge a superfície terrestre, especialmente a radiação ultravioletaB (UV-B). O aumento da radiação poderá induzir a mudanças estruturais e fisiológicas nas plantas, influenciando no seu crescimento e desenvolvimento. O objetivo deste trabalho foi determinar os efeitos da radiação UV-B ambiente sobre a morfologia das folhas de Arabidopsis thaliana desenvolvidas em condições controladas. As sementes de A. thaliana cresceram em câmaras de crescimento, com 300 µmol m-2s-1 de radiação fotossinteticamente ativa (PAR) com ou sem 6 kJ m-2 s-1 de radiação UV-Bbe (UV-Bbe; UV-B biologicamente efetiva). Após 21 dias, 10 folhas de cada tratamento (com e sem radiação UV-B) foram coletadas...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arabidopsis thaliana; Radiação UV-B; Morfologia foliar; Compostos fenólicos.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062006000200008

The Ca2+ pump inhibitor, thapsigargin, inhibits root gravitropism in Arabidopsis thaliana
Provedor de dados: 48
Autores: URBINA,DANIELA C; SILVA,HERMAN; MEISEL,LEE A.
Thapsigargin, a specific inhibitor of most animal intracellular SERCA-type Ca2+ pumps present in the sarcoplasmic/endoplasmic reticulum, was originally isolated from the roots of the Mediterranean plant Thapsia gargancia L. Here, we demonstrate that this root-derived compound is capable of altering root gravitropism in Arabidopsis thaliana. Thapsigargin concentrations as low as 0.1 µM alter root gravitropism whereas under similar conditions cyclopiazonic acid does not. Furthermore, a fluorescently conjugated thapsigargin (BODIPY FL thapsigargin) suggests that target sites for thapsigargin are located in intracellular organelles in the root distal elongation zone and the root cap, regions known to regulate root gravitropism
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Ca2+-ATPases; Calcium; Gravitropism; Thapsigargin; Root.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200011

Arabidopsis thaliana: A model host plant to study plant-pathogen interaction using Chilean field isolates of Botrytis cinerea
Provedor de dados: 48
Autores: GONZÁLEZ,JUAN; REYES,FRANCISCA; SALAS,CARLOS; SANTIAG,MARGARITA; CODRIANSKY,YAEL; COLIHEUQUE,NELSON; SILVA,HERMAN.
One of the fungal pathogens that causes more agriculture damage is Botrytis cinerea. Botrytis is a constant threat to crops because the fungus infects a wide range of host species, both native and cultivated. Furthermore, Botrytis persists on plant debris in and on the soil. Some of the most serious diseases caused by Botrytis include gray mold on vegetables and fruits, such as grapes and strawberries. Botrytis also causes secondary soft rot of fruits and vegetables during storage, transit and at the market. In many plant-pathogen interactions, resistance often is associated with the deposition of callose, accumulation of autofluorescent compounds, the synthesis and accumulation of salicylic acid as well as pathogenesis-related proteins. Arabidopsis...
Tipo: Journal article
Palavras-chave: Arabidopsis thaliana; Botrytis cinerea; Plant-pathogen interaction; PR genes.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602006000200004

Metabolite and light regulation of metabolism in plants: lessons from the study of a single biochemical pathway
Provedor de dados: 56
Autores: Oliveira,I.C.; Brenner,E.; Chiu,J.; Hsieh,M.-H.; Kouranov,A.; Lam,H.-M.; Shin,M.J.; Coruzzi,G.
We are using molecular, biochemical, and genetic approaches to study the structural and regulatory genes controlling the assimilation of inorganic nitrogen into the amino acids glutamine, glutamate, aspartate and asparagine. These amino acids serve as the principal nitrogen-transport amino acids in most crop and higher plants including Arabidopsis thaliana. We have begun to investigate the regulatory mechanisms controlling nitrogen assimilation into these amino acids in plants using molecular and genetic approaches in Arabidopsis. The synthesis of the amide amino acids glutamine and asparagine is subject to tight regulation in response to environmental factors such as light and to metabolic factors such as sucrose and amino acids. For instance, light...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amino acids; Carbon; Ammonium; Nitrogen assimilation; Gene expression; Arabidopsis thaliana.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000500003