Consulta: subjectFacets:"Biosensors"
Registros recuperados: 7
Data/hora: 15/06/2024 16:31:34

02SRC154
Provedor de dados: 8
Autores: Allen, David W.; Coles, Simon J.; Hursthouse, Michael B.
Tipo: Crystal Structure Data Holding
Palavras-chave: C~33~H~~25~BrNO~2~P; Organic; Biosensors; Polar compounds organic; Electronic materials.
Ano: 2002
URL: http://ecrystals.chem.soton.ac.uk/878/

The application of polymerized lipid vesicles as colorimetric biosensors for real-time detection of pathogens in drinking water
Provedor de dados: 69
Autores: Villalobos,Patricio; Chávez,María Isabel; Olguín,Yusser; Sánchez,Elizabeth; Valdés,Erika; Galindo,Raúl; Young,Manuel E.
The inadequate treatments given to the served waste water which are disposal to the rivers and sea coast are the major sources of faecal Microorganisms and enteric bacterial pathogens. They are among the most serious effects of water pollution bringing risks on public health. None of the current methods for detection of pathogens offer real-time on site solutions, are capable of delivering a simple visual detection signal, or can be easily instrumented as an indicator of the presence of a pathogen in water. The use of lipid vesicles incorporating Polydiacetylenes (PDAs) for the development of biosensors for &amp;#147;real-time&amp;#148; detection of pathogens has become an alternative, due to its potential for simple colorimetric response against harmful...
Tipo: Journal article
Palavras-chave: Bacterial detection; Biosensors; Lipid vesicles; PDA.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000100004

Assessment of molecular recognition element for the quantification of human epidermal growth factor using surface plasmon resonance
Provedor de dados: 69
Autores: Rosti,Ira Amira; Ramanan,Ramakrishnan Nagasundara; Ling,Tau Chuan; Ariff,Arbakariya B.
Background: A method for the selection of suitable molecular recognition element (MRE) for the quantification of human epidermal growth factor (hEGF) using surface plasmon resonance (SPR) is presented. Two types of hEGF antibody, monoclonal and polyclonal, were immobilized on the surface of chip and validated for its characteristics and performance in the quantification of hEGF. Validation of this analytical procedure was to demonstrate the stability and suitability of antibody for the quantification of target protein. Results: Specificity, accuracy and precision for all samples were within acceptable limit for both antibodies. The affinity and kinetic constant of antibodies-hEGF binding were evaluated using a 1:1 Langmuir interaction model. The model...
Tipo: Journal article
Palavras-chave: Antibody; BIAcore; Biosensors; Epidermal growth factor; Validation.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000600015

DNA hybridization mechanism in an interfacial environment: What hides beneath first order k (s(-1)) kinetic constant?
Provedor de dados: 5
Autores: Lazerges, M.; Perrot, H.; Rabehagasoa, N.; Compere, Chantal; Dreanno, Catherine; Mucio Pedroso, M.; Faria, R. C.; Bueno, P. R.
The scientific question addressed in this work is: what hides beneath first order kinetic constant k (s(-1)) measured for hybridization of a DNA target on a biosensor surface. Kinetics hybridization curves were established with a 27 MHz quartz microbalance (9 MHz, third harmonic) biosensor, constituted of a 20-base probe monolayer deposited on a gold covered quartz surface. Kinetics analysis, by a known two-step adsorption-hybridization mechanism, is well appropriate to fit properly hybridization kinetics curves, for complementary 20-base to 40-base targets over two concentration decades. It was found that the K-1 (M-1) adsorption constant, relevant to the first step, concerns an equilibrium between non hybridized targets and hybridized pre-complex and...
Tipo: Text
Palavras-chave: Biosensors; DNA; Hybridization kinetics; Quartz crystal microbalance.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00101/21195/19415.pdf

Enzymes immobilized in Langmuir-Blodgett films: Why determining the surface properties in Langmuir monolayer is important?
Provedor de dados: 42
Autores: CASELI,LUCIANO.
ABSTRACT In this review we discuss about the immobilization of enzymes in Langmuir-Blodgett films in order to determine the catalytic properties of these biomacromolecules when adsorbed on solid supports. Usually, the conformation of enzymes depends on the environmental conditions imposed to them, including the chemical composition of the matrix, and the morphology and thickness of the film. In this review, we show an outline of manuscripts that report the immobilization of enzymes as LB films since the 1980’s, and also some examples of how the surface properties of the floating monolayer prepared previously to the transfer to the solid support are important to determine the efficiency of the resulting device.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Langmuir-Blodgett; Enzymes; Biosensors; Air-water interface.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652018000200631

Electrochemical biosensors in pharmaceutical analysis
Provedor de dados: 60
Autores: Gil,Eric de Souza; Melo,Giselle Rodrigues de.
Given the increasing demand for practical and low-cost analytical techniques, biosensors have attracted attention for use in the quality analysis of drugs, medicines, and other analytes of interest in the pharmaceutical area. Biosensors allow quantification not only of the active component in pharmaceutical formulations, but also the analysis of degradation products and metabolites in biological fluids. Thus, this article presents a brief review of biosensor use in pharmaceutical analysis, focusing on enzymatic electrochemical sensors.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biosensors; Pharmaceutical analysis; Electrochemical sensors; Enzymes.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502010000300002

Use of Enzymatic Biosensors as Quality Indices: A Synopsis of Present and Future Trends in The Food Industry
Provedor de dados: 117
Autores: Serna Cock,Liliana; Zetty Arenas,Ana María; Ayala Aponte,Alfredo.
Biosensors are an important alternative in the food industry to ensure the quality and safety of products and process controls with effective, fast and economical methods. Their technology is based on a specific biological recognition element in combination with a transducer for signal processing. The use of enzymatic biosensor technology in food processing, quality control and on-line processes is promising compared to conventional analytical techniques, as it offers great advantages due to size, cost, specificity, fast response, precision and sensitivity. This article reviews the development and use of some enzyme biosensors in the food industry, describes the most important application areas and analyzes the current situation and future possibilities....
Tipo: Journal article
Palavras-chave: Biosensors; Enzymes; Analysis in food; Safety; Quality; Control processes.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392009000200017