Consulta: subjectFacets:"Bromoindirubin oxime"
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Data/hora: 03/06/2024 01:49:08

6-Bromoindirubin-3’-oxime promotes osteogenic differentiation of canine BMSCs through inhibition of GSK3β activity and activation of the Wnt/β-catenin signaling pathway
Provedor de dados: 42
Autores: ZHAO,XIAO-E; YANG,ZHENSHAN; GAO,ZHEN; GE,JUNBANG; WEI,QIANG; MA,BAOHUA.
﻿﻿This﻿﻿ study aimed to investigate how 6-bromoindirubin-3’-oxime (BIO) increases the osteogenic differentiation of canine bone mesenchymal stem cells (BMSCs) and the role of the Wnt/β-catenin signaling pathway in this process. We mimicked the effect of Wnt by adding BIO to the culture medium of BMSCs and examined whether canonical Wnt signaling positively affects the differentiation of these cells into osteoblasts. Canine BMSCs were cultured with 0.5 and 1.0 μM BIO under osteogenic conditions and then differentiation markers were investigated. It was found that BIO significantly increased the activity of alkaline phosphatase (ALP), the number of ALP-positive cells, the mineralization level and calcium deposits. Moreover, cells cultured with 0.5 and 1.0 μM...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bone mesenchymal stem cells; Bromoindirubin oxime; Osteogenetic differentiation; Canine.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000100610