Consulta: subjectFacets:"CDNA"
Registros recuperados: 14
Data/hora: 03/06/2024 04:36:28

Suppression subtractive hybridization PCR isolation of cDNAs from a Caribbean soft coral
Provedor de dados: 69
Autores: Lopez,Jose V; Ledger,Angela; Santiago-Vázquez,Lory Z; Pop,Mihai; Sommer,Dan D; Ranzer,Llanie K; Feldman,Robert A; Russell,G. Kerr.
Transcriptomic studies of marine organisms are still in their infancy. A partial, subtracted expressed sequence tag (EST) library of the Caribbean octocoral Erythropodium caribaeorum and the sea fan Gorgonia ventalina has been analyzed in order to find novel genes or differences in gene expression related to potential secondary metabolite production or symbioses. This approach entails enrichment for potential non-&amp;#8220;housekeeping&amp;#8221; genes using the suppression subtractive hybridization (SSH) polymerase chain reaction (PCR) method. More than 500 expressed sequence tags (ESTs) were generated after cloning SSH products, which yielded at least 53 orthologous groups of proteins (COGs) and Pfam clusters, including transcription factors (Drosophila...
Tipo: Journal article
Palavras-chave: CDNA; Erythropodium caribaeorum; EST; Gorgonia ventalina; Gorgonian; Sea fan.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100008

Molecular cloning, expression and characterization of a serine proteinase from Japanese edible mushroom, Grifola frondosa: solving the structure - function anomaly of a reported aminopeptidase
Provedor de dados: 69
Autores: Islam,Mohammed M.
The N-terminal amino acid sequence of an aminopeptidase from Japanese edible mushroom, Grifola frondosa, was reported to have high similarity with that of a serine proteinase from basidiomycete, Agaricus bisporous (Nishiwaki and Hayashi, 2001). The full-length cDNA and the corresponding genomic DNA of the enzyme were cloned, based on the reported N-terminal amino acid sequence. The predicted open reading frame (ORF) of the cloned cDNA, encoding a product of 379 amino acids, was expressed in E. coli using pET expression vector. The expressed pro-enzyme (40 kDa) underwent autolysis to produce the mature protein (30 kDa) and a pro-peptide (10 kDa). The mature protein and the pro-peptide remained tightly bound to each other and could not be separated by Ni-NTA...
Tipo: Journal article
Palavras-chave: 5'-RACE; CDNA; Genomic DNA; Maitake; Refolding.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400010

The lift pool method for isolation of cDNA clones from lambda phage libraries
Provedor de dados: 69
Autores: LeBlanc-Straceski,Janine; Sobrado,Pablo; Betz,Sharon; Zerfas,Julie; Morgan,Karen.
A PCR based strategy was developed to screen a Xenopus oocyte &amp;lambda;gt10 cDNA library. The PCR-based lift pool (LP) method follows the same two tiered strategy as conventional screening of phage libraries by filter hybridization. Two rounds of plating, one at high density to detect the clone, and one at low density to purify the clone to homogeneity, are performed. In the first round, lysates from high density plates, termed plate pools (PP), serve as template for PCR. In the second round, phage particles adhering to plaque lifts of low density plates are washed off nitrocellulose filters to create LPs, which are used as template for PCR. The integrity of the plaques on the low-density plates is preserved. Once a positive LP has been identified,...
Tipo: Journal article
Palavras-chave: CDNA; Lambda; Non-radioactive; PCR; Plaque; Screening.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400012

A growth hormone-based phylogenetic analysis of euteleostean fishes including a representative species of the Atheriniformes Order, Odontesthes argentinensis
Provedor de dados: 74
Autores: Marins,Luis F.; Levy,Jose A.; Folch,Josep M.; Sanchez,Armand.
The GH (growth hormone) cDNA sequence of the marine silverside fish Odontesthes argentinensis was obtained using the RACE protocol (Rapid Amplification of cDNA Ends). The marine silverside GH cDNA sequence is 928 nucleotides long and was found to encode a polypeptide of 204 amino acids, including a signal peptide of 17 amino acids. The 5' and 3' untranslated regions of the messenger are 109 and 204 nucleotides long, respectively. The deduced GH amino acid sequence was used to infer a phylogenetic tree with GH amino acid sequences from representative species belonging to the Euteleostei Subdivision using the maximum parsimony method. The topology found is according to the major phylogenetic grouping of euteleosts. The results corroborate the hypothesis that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Growth hormone; CDNA; RACE (Rapid Amplification of cDNA Ends); Phylogeny.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300013

Molecular characterization of MHC-DRB cDNA in water buffalo (Bubalus bubalis)
Provedor de dados: 74
Autores: Naskar,Soumen; Deb,Sitangsu M.; Niranjan,Saket K.; Kumar,Subodh; Sharma,Deepak; Sakaram,Durgam; Sharma,Arjava.
In the present study, water buffalo MHC (Bubu)-DRB cDNA was cloned and characterized. The 1022 base long-amplified cDNA product encompassed a single open reading frame of 801 bases that coded for 266 amino acids. The Bubu-DRB sequence showed maximum homology with the BoLA-DRB3*0101 allele of cattle. A total of seven amino acid residues were found to be unique for the Bubu-DRB sequence. The majority of amino acid substitutions was observed in the β1 domain. Residues associated with important functions were mostly conserved. Water buffalo DRB was phylogenetically closer to goat DRB*A.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Water buffalo; CDNA; DRB; MHC; Phylogeny.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100013

Three synonymous genes encode calmodulin in a reptile, the Japanese tortoise, Clemmys japonica
Provedor de dados: 74
Autores: Shimoda,Kouji; Miyake,Toshihiro; Kimura,Jun; Maejima,Kazuyoshi.
Three distinct calmodulin (CaM)-encoding cDNAs were isolated from a reptile, the Japanese tortoise (Clemmys japonica), based on degenerative primer PCR. Because of synonymous codon usages, the deduced amino acid (aa) sequences were exactly the same in all three genes and identical to the aa sequence of vertebrate CaM. The three cDNAs, referred to as CaM-A, -B, and -C, seemed to belong to the same type as CaMI, CaMII, and CaMIII, respectively, based on their sequence identity with those of the mammalian cDNAs and the glutamate codon biases. Northern blot analysis detected CaM-A and -B as bands corresponding to 1.8 kb, with the most abundant levels in the brain and testis, while CaM-C was detected most abundantly in the brain as bands of 1.4 and 2.0 kb. Our...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Reptile; CDNA; Synonymousgenes.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000100009

Molecular manipulation and modification of the genes encoding the G2 and G4 glycinin subunits
Provedor de dados: 74
Autores: Sammour,Reda H.
The genes encoding the glycinin subunits G2 and G4 were molecularly manipulated and modified to test the possibility of increasing the nutritional value of soybean seed proteins. The recombinant DNAs pSP65/G2HG4, pSP65/G4HG2, pSP65/248 Metl, pSP65/248 Met2,3 and pSP65/248 Metl.2,3 were used in in vitro translation to produce (i) chimeric proteins consisting of reciprocally exchanged acidic and basic G2 and G4 domains and (ii) Gy4 point mutants with an increased number of methionine residues. The ability of the recombinant proteins to assemble into proper quaternary structures was investigated using sucrose gradient fractionation. The data produced by this study could provide valuable clues for the potential improvement of genetically modified crops.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNA; Glycinin subunit G4; In vitro translation; Mutagenesis; Soybean.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300025

Sequence analysis of the cDNA encoding for SpCTx: a lethal factor from scorpionfish venom ( Scorpaena plumieri )
Provedor de dados: 80
Autores: Costa,Fábio L. S.; Lima,Maria Elena De; Figueiredo,Suely G.; Ferreira,Rafaela S.; Prates,Núbia S.; Sakamoto,Tetsu; Salas,Carlos E.
Abstract  Background: Lethal factors are multifunctional oligomeric proteins found in the venomous apparatus of Scorpaeniformes fish. These toxins elicit not only an array of biological responses in vitro but also cardiovascular disorders and strong hemolytic, nociceptive and edematogenic activities in vivo. This work describes the cloning and molecular identification of two toxin subunits, denominated Sp-CTx-α and Sp-CTx-β, from scorpionfish venom ( Scorpaena plumieri ).  Methods: The primary structures were deduced after cDNA amplification by PCR with primers from conserved sequences described in Scorpaeniformes toxins. Following DNA sequencing and bioinformatic analysis, the tridimensional structures of both subunits were modeled.  Results: The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: CDNA; Lethal factor; Scorpaena plumieri; Scorpionfish; Venom gland.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992018000100315

アズキ品種のアスコルビン酸ペルオキシダーゼの比較
Provedor de dados: 19
Autores: 小嶋, 道之; 齋藤, 優介; 山下, 慎司; Kojima, Michiyuki; Yamashita, Shinji; Saito, Yusuke.
アズキ(Vigna angularis (Willd.) Ohwi et H.Ohashi：品種はエリモショウズ)に含まれるアスコルビン酸ペルオキシダーゼ(APX)をコードするcDNAの塩基配列を決定した。APXはアスコルビン酸を基質として細胞毒である過酸化水素を水に無毒化する酵素である。アズキAPX遺伝子の塩基数は1,076bpで，予想されるタンパク質のアミノ酸数は250，分子量は約27,000Daであった。アズキAPXのアミノ酸配列は，ササゲ，ダイズおよびエンドウのそれらと高い相同性を示し，順に98%，95%および92%であった。また、アズキ5品種の初生葉からAPXをコードするcDNAを調製して，塩基配列を比較した。その結果として，(1)エリモショウズのAPX遺伝子ORFから6 –10番目の塩基配列は“TGCTA”であったが，他の4品種のそれは“AGTAC”であること，(2)APX遺伝子ORFから61番目の塩基はエリモショウズ，斑小粒系-1およびトヨミダイナゴンでは“T”であったが，アカネダイナゴンおよびサホロショウズでは“C”であることを明らかにした。
Palavras-chave: アスコルビン酸ペルオキシダーゼ; アスコルビン酸; CDNA; アズキ; Ascorbate peroxidase(APX); Ascorbie acid; CDNA; Adzuki bean.
Ano: 2006
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/95

Sequence Analysis of Three Major Antigens (P30, P23 and P22) of Virulent and Avirulent Strains of Toxoplasma gondii
Provedor de dados: 19
Autores: Maki, Yoshiyuki; Seng, Seyha; Kato, Mihoko; Hoshi, Yukihiro; Igarashi, Ikuo; Nagasawa, Hideyuki; Toyoda, Yutaka; Suzuki, Naoyoshi.
Palavras-chave: Toxoplasma gondii; SAG-1 (P30); CDNA.
Ano: 1996
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/250

Código de barras de ADN  de las especies cubanas de peces  dulceacuícolas.
Provedor de dados: 20
Autores: Lara Lorenzo, A.
En el presente trabajo se realizó el análisis del código de barras ADN de 24 especies de la  ictiofauna dulceacuícola cubana. Para ello,  se secuenciaron 652pb del gen mitocondrial  citocromo oxidasa I (COI) de 96 individuos provenientes de diferentes áreas geográficas y  representativos, en la mayoría de los casos, de la distribución espacial de cada especie. En  el análisis se utilizó la distancia genética Kimura 2 parámetros (K2P), considerando como  cota superior el valor de 3%, para identificar los límites de divergencia entre especies.  Complementariamente se llevó a cabo un análisis de agregación poblacional para  identificar grupos poblacionales  indicativos de linajes evolutivos independientes. El  estimado promedio de divergencia genética entre...
Tipo: Theses and Dissertations
Palavras-chave: CDNA; Genes; Fishes; Fish wastes.
Ano: 2008
URL: http://hdl.handle.net/1834/5514

RNA fingerprinting using RAP-PCR identifies an EBAF homologue mRNA differentially expressed in rat oviduct
Provedor de dados: 47
Autores: Valdecantos,Pablo A.; Argañaraz,Martín E.; Abate,Carlos M.; Miceli,Dora C.
As a step towards the identification of genes preferentially expressed in the oviduct during early rat embryo development, we isolated a cDNA fragment (Pr14) by using RNA arbitrarily primed PCR (RAP-PCR), being its expression restricted to oviduct and uterus; its mRNA is mainly expressed in oviduct during late luteal phase and early pregnancy. This fragment is 100% identical to a rat DNA sequence (Accession No. NW_047400) downstream the terminal exon of a Rattus norvegicus gene (Locus Link Accession No. LOC289316) similar to ebaf (endometrial bleeding-associated factor), a novel member of the Transforming Growth Factor superfamily. Northern analyses showed that this sequence hybridizes with 2.9 kb and 4.1 kb mRNAs in early pregnant rat oviducts. However,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Differential gene expression; Oviduct; RNA fingerprint; CDNA.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452004000300005

cDNA and deduced primary structure of basic phospholipase A2 with neurotoxic activity from the venom secretion of the Crotalus durissus collilineatus rattlesnake
Provedor de dados: 56
Autores: Fagundes,F.H.R.; Oliveira,M.; Huancahuire-Vega,S.; Romero-Vargas,F.F.; Ponce-Soto,L.A.; Marangoni,S.
To illustrate the construction of precursor complementary DNAs, we isolated mRNAs from whole venom samples. After reverse transcription polymerase chain reaction (RT-PCR), we amplified the cDNA coding for a neurotoxic protein, phospholipase A2 D49 (PLA2 D49), from the venom of Crotalus durissus collilineatus (Cdc PLA2). The cDNA encoding Cdc PLA2 from whole venom was sequenced. The deduced amino acid sequence of this cDNA has high overall sequence identity with the group II PLA2 protein family. Cdc PLA2 has 14 cysteine residues capable of forming seven disulfide bonds that characterize this group of PLA2 enzymes. Cdc PLA2 was isolated using conventional Sephadex G75 column chromatography and reverse-phase high performance liquid chromatography (RP-HPLC)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cdc PLA2; CDNA; Chick biventer cervicis; Crotalus durissus collilineatus; Mass spectrometry MALDI-TOF; Neurotoxic agents.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000300007

Brazilian coffee genome project: an EST-based genomic resource
Provedor de dados: 61
Autores: Vieira,Luiz Gonzaga Esteves; Andrade,Alan Carvalho; Colombo,Carlos Augusto; Moraes,Ana Heloneida de Araújo; Metha,Ângela; Oliveira,Angélica Carvalho de; Labate,Carlos Alberto; Marino,Celso Luis; Monteiro-Vitorello,Claúdia de Barros; Monte,Damares de Castro; Giglioti,Éder; Kimura,Edna Teruko; Romano,Eduardo; Kuramae,Eiko Eurya; Lemos,Eliana Gertrudes Macedo; Almeida,Elionor Rita Pereira de; Jorge,Érika C.; Albuquerque,Érika V. S.; Silva,Felipe Rodrigues da; Vinecky,Felipe; Sawazaki,Haiko Enok; Dorry,Hamza Fahmi A.; Carrer,Helaine; Abreu,Ilka Nacif; Batista,João A. N.; Teixeira,João Batista; Kitajima,João Paulo; Xavier,Karem Guimarães; Lima,Liziane Maria de; Camargo,Luis Eduardo Aranha de; Pereira,Luiz Filipe Protasio; Coutinho,Luiz Lehmann; Lemos,Manoel Victor Franco; Romano,Marcelo Ribeiro; Machado,Marcos Antonio; Costa,Marcos Mota do Carmo; Sá,Maria Fátima Grossi de; Goldman,Maria Helena S.; Ferro,Maria Inês T.; Tinoco,Maria Laine Penha; Oliveira,Mariana C.; Van Sluys,Marie-Anne; Shimizu,Milton Massao; Maluf,Mirian Perez; Eira,Mirian Therezinha Souza da; Guerreiro Filho,Oliveiro; Arruda,Paulo; Mazzafera,Paulo; Mariani,Pilar Drummond Sampaio Correa; Oliveira,Regina L.B.C. de; Harakava,Ricardo; Balbao,Silvia Filippi; Tsai,Siu Mui; Mauro,Sonia Marli Zingaretti di; Santos,Suzana Neiva; Siqueira,Walter José; Costa,Gustavo Gilson Lacerda; Formighieri,Eduardo Fernandes; Carazzolle,Marcelo Falsarella; Pereira,Gonçalo Amarante Guimarães.
Coffee is one of the most valuable agricultural commodities and ranks second on international trade exchanges. The genus Coffea belongs to the Rubiaceae family which includes other important plants. The genus contains about 100 species but commercial production is based only on two species, Coffea arabica and Coffea canephora that represent about 70 % and 30 % of the total coffee market, respectively. The Brazilian Coffee Genome Project was designed with the objective of making modern genomics resources available to the coffee scientific community, working on different aspects of the coffee production chain. We have single-pass sequenced a total of 214,964 randomly picked clones from 37 cDNA libraries of C. arabica, C. canephora and C. racemosa,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea; CDNA; EST; Transcriptome.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202006000100008