Consulta: subjectFacets:"Genetic diversity"
Registros recuperados: 293
Data/hora: 01/06/2024 02:37:08

Diversidad genética, potencial agronómico y de calidad en maíces pigmentados de Valles Altos de México.
Provedor de dados: 32
Autores: García Perea, Marco Antonio.
México cuenta con una gran diversidad genética de maíz que es manejada y conservada por pequeños productores que cultivan tradicionalmente un mosaico de variantes, entré estas se encuentran maíces con granos de color azul y rojo, a los que se les han conferido características nutracéuticas por su contenido de antocianinas y calidad de proteína, además de su sabor. Las estrategias de conservación y aprovechamiento in situ de la diversidad genética del maíz requieren valorar tanto la diversidad como al potencial para incrementar la producción y calidad bajo el esquema de la agricultura tradicional, como punto de referencia. Se consideró estudiar la diversidad genética particularizando en las variantes de maíz nativo con granos de color azul y rojo de los...
Palavras-chave: Zea mays; Diversidad genética; Maíces nativos pigmentados; Potencial agronómico; Calidad de proteína; Valles Altos; Genetic diversity; Pigmented native maize; Agronomic potential; Protein quality; High valleys; Genética; Doctorado.
Ano: 2012
URL: http://hdl.handle.net/10521/1842

Diversidad de maíz potencial agronómico y perspectivas para su conservación y desarrollo in situ, en el sureste del estado de México
Provedor de dados: 32
Autores: González González, Miguel.
Para diseñar estrategias de conservación in situ de la diversidad genética del maíz, se requiere de valorar tanto a la diversidad como al potencial para incrementar la producción bajo el esquema de la agricultura tradicional como punto de referencia. Dado que el intercambio de semilla se da principalmente dentro de las comunidades rurales, se consideró al municipio de Ayapango, Edo. de Méx., para colectar las poblaciones de maíz que se manejan en aproximadamente el 20% de los hogares. Se colectaron 97 poblaciones de la raza Chalqueño, y dos de Cacahuacintle. Dentro de Chalqueño hubo poblaciones del tipo de grano cremoso (49), del conocido localmente como Palomo (19), del azul (24), del rojo (4) denominado localmente como “xitocle”, y una población de...
Tipo: Tesis
Palavras-chave: Diversidad genética; Maíz; Chalqueño; Conservación in situ; Isoenzimas; Doctorado; Genética; Genetic diversity; Maize; Chalqueño; In situ conservation; Isozymes.
Ano: 2007
URL: http://hdl.handle.net/10521/1585

Diversidad genética del maíz, perspectivas para su conservación y desarrollo en una comunidad mixteca de oaxaca: Santa María Tataltepec.
Provedor de dados: 32
Autores: Muñozcano Ruiz, Máximo.
Valorar la diversidad genética y evaluar el potencial agronómico de las poblaciones de maíz nativas de Santa María Tataltepec, Oaxaca, fueron los principales objetivos de esta investigación, como una vía para fomentar el desarrollo regional. Se colectaron 69 poblaciones de maíz nativo (criollo), 65 de Tataltepec y 4 de otras comunidades de la Mixteca; de las 65 poblaciones locales (en 75% de los hogares), 44 fueron de grano blanco, 11 de azul y 10 de amarillo. En el verano del 2010 se establecieron experimentos de campo con repeticiones, en condiciones de temporal. Para valorar la diversidad se caracterizaron las poblaciones mediante siete, diez y cinco variables morfológicas de planta, mazorca y grano, respectivamente y se aplicaron análisis de...
Palavras-chave: Zea mays L.; Bolita; Diversidad genética; Maíces nativos; Pepitilla; Potencial agronómico; Genetic diversity; Native populations (Landraces); Genética; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/596

Resistencia de clones de papa al tizón tardío (Phytophthora infestans) y su caracterización morfológica en el Valle de Toluca.
Provedor de dados: 32
Autores: Rivadeneira Ruales, Jorge Esteban.
El rendimiento, la resistencia a enfermedades y la calidad del tubérculo son las características más importantes en el mejoramiento genético de la papa (Solanum tuberosum); por ello, el objetivo de la presente investigación fue estudiar los componentes de varianza y heredabilidad en sentido estrecho (h2) de la respuesta a Phytophthora infestans, así como la caracterización morfológica de clones resistentes, así como seleccionar los clones con resistencia y buenas características agronómicas. Se evaluaron 198 clones en primera generación clonal de 20 cruzas, y 13 de los 20 progenitores en el campo experimental del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), en el valle de Toluca, Estado de México. Más del 30% de clones...
Palavras-chave: Solanum tuberosum; Phytophthora infestans; Resistencia; Tizón tardío; Heredabilidad; Diversidad genética; Clones; Cruzas; Progenitor; Resistance; Late blight; Heritability; Genetic diversity; Clones; Crosses; Parent; Maestría; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/505

Diversidad del jitomate nativo de Puebla y Veracruz : valoración morfo-agronómica y germinación en medio salino.
Provedor de dados: 32
Autores: Estrada Trejo, Victoria.
México ha avanzado poco en el estudio, conservación, aprovechamiento sistemático y mejoramiento genético del jitomate nativo mexicano. También hace falta conocer las respuestas de este germoplasma a condiciones de algún tipo de estrés ya sea biótico o abiótico. Con tal fin en la primera parte de este estudio, el objetivo fue conocer la diversidad agronómica y morfológica del jitomate nativo mexicano, con miras a su incorporación en programas de mejoramiento genético. Se caracterizaron morfológica y agronómicamente 34 poblaciones nativas de jitomate, provenientes de los estados de Puebla y Veracruz, México. Los resultados de los estimadores de componentes de varianza indicaron que de las 43 variables, únicamente 16 mostraron repetibilidad, y 27...
Palavras-chave: Solanum lycopersicum L.; Salinidad; Cloruro de sodio; Diversidad genética; Recursos genéticos de jitomate; Salinity; Sodium chloride; Genetic diversity; Tomato genetic resources; Producción de semillas; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2127

Diversidad genética y potencial de poblaciones criollas de maíz del centro - sur de Tamaulipas, México
Provedor de dados: 32
Autores: Pecina Martínez, José Agapito.
Se ha demostrado el potencial que tienen las poblaciones criollas de maíz de Tamaulipas;  sin embargo, éstas no se han aprovechado eficientemente, debido al escaso trabajo de  investigación, especialmente en mejoramiento genético realizado en el Estado. Por lo tanto,  es necesario preservar y explorar aún más la diversidad genética y seguir generando  conocimiento que permita definir estrategias y acciones para evitar su erosión y su posible  contaminación con maíces transgénicos que se introduzcan al país. Con base en lo anterior,  desde el 2001, en la Unidad Académica Multidisciplinaria de Agronomía y Ciencias de la  Universidad Autónoma de Tamaulipas se implementó un programa de rescate, conservación  y manejo de germoplasma criollo de maíz. De más de...
Palavras-chave: Maíz criollo de Tamaulipas; Diversidad genética; Aprovechamiento de la diversidad; Mejoramiento de maíz; Indicadores de respuesta ambiental. landraces of maize from Tamaulipas; Genetic diversity; Use of the diversity; Maize breeding; Environmental response indicators.
Ano: 2012
URL: http://hdl.handle.net/10521/876

Variabilidad genética del mildiu (Bremia lactucae Regel) en Salamanca y San Miguel de Allende, Guanajuato, mediante RAPD e ISSR
Provedor de dados: 32
Autores: Martínez Martínez, Susana.
Bremia lactucae Regel causa una de las enfermedades de mayor importancia en la lechuga a nivel mundial. Debido a que este pseudohongo presenta dos tipos de reproducción: sexual y asexual, su diversidad genética es amplia y puede dar origen a nuevas razas. Dicha situación se desconoce en México, por lo que es importante conocer este aspecto en las zonas productoras de esta hortaliza, así como también la influencia que ejercen los factores ambientales sobre la incidencia y severidad del patógeno. Por ello, fueron determinadas la incidencia y severidad de B. lactucae en 15 variedades de lechuga, además se registraron variables ambientales como la temperatura, humedad relativa, humedad de la hoja y precipitación en los municipios de Salamanca y San Miguel de...
Tipo: Tesis
Palavras-chave: Bremia lactucae; Mildiu; Diversidad genética; Microsatélites; Maestría; Fitopatología; Mildew; Genetic diversity; Microsatellite.
Ano: 2008
URL: http://hdl.handle.net/10521/1675

Caracterización, rendimiento de maíces nativos y descripción de las unidades de producción en el Municipio de Molcaxac, Pue.
Provedor de dados: 32
Autores: Angeles Gaspar, Elizabeth.
El maíz (Zea mays L.) en México es importante social, cultural y económicamente. En el municipio de Molcaxac, Pue. la mayor parte de los productores utilizan poblaciones locales de maíz. Sin embargo, no existen estudios en la zona que describan la diversidad y potencial de las poblaciones locales de maíz, y las condiciones socioeconómicas en que se da la producción de este grano. La presente tesis se dividió en dos partes. La primera  tuvo como objetivos evaluar el rendimiento de grano y caracterizar agronómicamente la diversidad de las poblaciones locales de maíz existentes en el municipio de Molcaxac. La segunda parte tuvo como objetivo realizar una descripción general de las principales características de los productores de maíz, así como de su unidad...
Palavras-chave: Zea mays L.; Poblaciones locales; Diversidad genética; Sistmemas de producción; Local populations; Genetic diversity; Production systems; Maestría; Estrategías para el Desarrollo Agrícola Regional; EDAR.
Ano: 2010
URL: http://hdl.handle.net/10521/177

Caracterización agronómica y denominación de poblaciones locales de maíz (Zea mays L.) de la comunidad de Cualac, Guerrero.
Provedor de dados: 32
Autores: Jiménez Tapia, Silvino.
La mayoría de la superficie agrícola en Cualac, estado de Guerrero, es sembrada con poblaciones locales de maíz, las cuales, hasta el momento no han sido caracterizadas a nivel de comunidad. El objetivo del presente trabajo, fue  colectar muestras representativas de la diversidad de maíz de la comunidad de Cualac y su caracterización agronómica. En 2007, se evaluaron 25 genotipos, 23 fueron poblaciones de Cualac y dos tipos mejorados, el H-507 y VS-535. Se utilizó un diseño de látice 5 x 5, con cuatro repeticiones. Los caracteres evaluados, mostraron diferencias entre poblaciones (p&lt;0.05), lo que muestra riqueza en diversidad. En rendimiento de grano, existen poblaciones nativas que igualan (P&lt;0.05) a los materiales comerciales recomendados para la...
Palavras-chave: Zea mays; Poblaciones locales; Diversidad genética; Color de grano; Local populations; Genetic diversity; Grain color; EDAR; Desarrollo Sostenible de Zonas Indígenas; Maestría Tecnológica.
Ano: 2010
URL: http://hdl.handle.net/10521/304

Diversidad genética y patrón de cruzamiento en poblaciones naturales de Pseudotsuga menziesii (Mirb.) Franco
Provedor de dados: 32
Autores: Cruz Nicolas, Jorge.
Se determinó la estructura de la diversidad genética y el patrón de cruzamiento en poblaciones naturales de Pseudotsuga menziesii (Mirb.) Franco en México con base en información de isoenzimas. Para evaluar la diversidad genética se utilizó una muestra de 11 poblaciones naturales distribuidas en diferentes regiones geográficas (noroeste, noreste y centro) del país; el patrón de cruzamiento y su efecto sobre el nivel de endogamia sólo se evaluó en una muestra de tres poblaciones, representativas de cada región geográfica. Se encontró una amplia diversidad genética a nivel de especie (83.3% de loci polimórficos y un promedio de 2.9 alelos por locus), pero reducida a nivel de poblaciones (28.3 % de loci polimórficos y 1.52 alelos por locus), especialmente en...
Tipo: Tesis
Palavras-chave: Abeto Douglas; Distancias genéticas; Diversidad genética; Endogamia; Isoenzimas; Polimorfismo; Polinización cruzada; Maestría; Genética; Cross-pollination; Douglas-fir; Genetic distances; Genetic diversity; Inbreeding; Isozymes; Polymorphism.
Ano: 2007
URL: http://hdl.handle.net/10521/1587

Valoración agronómica y de calidad de fruto en poblaciones nativas de jitomate mexicano
Provedor de dados: 32
Autores: Flores Sánchez, Ignacio Darío.
México cuenta con una amplia diversidad en recursos fitogenéticos de poblaciones nativas de jitomate (Lycopersicon esculentum Mill.). El objetivo de la investigación fue evaluar la variabilidad morfológica en estado de plántula, características agronómicas relevantes y calidad de fruto, de 18 poblaciones nativas cultivadas en invernadero e hidroponía, provenientes de siete estados de la República y de la región Puebla-Hidalgo; como testigo se utilizó el jitomate comercial Sun-7705 (Nunhems®, tipo saladette). Se realizó en dos ciclos, en el primero, se evaluaron características morfológicas en plántula, con base en nueve grupos de origen. Se utilizó un diseño experimental completamente al azar con cinco repeticiones. Los datos se analizaron mediante...
Palavras-chave: Lycopersicum esculentum; Diversidad genética; Calidad de fruto; Invernadero; Hidroponia; Genetic diversity; Fruit quality; Greenhouse; Hydroponics; Genética; Maestría.
Ano: 2012
URL: http://hdl.handle.net/10521/1683

Caracterización, rendimiento de maíces nativos y descripción de las unidades de producción en el Municipio de Molcaxac, Pue.
Provedor de dados: 32
Autores: Ángeles Gaspar, Elizabeth.
El maíz (Zea mays L.) en México es importante social, cultural y económicamente. En el municipio de Molcaxac, Pue. la mayor parte de los productores utilizan poblaciones locales de maíz. Sin embargo, no existen estudios en la zona que describan la diversidad y potencial de las poblaciones locales de maíz, y las condiciones socioeconómicas en que se da la producción de este grano. La presente tesis se dividió en dos partes. La primera  tuvo como objetivos evaluar el rendimiento de grano y caracterizar agronómicamente la diversidad de las poblaciones locales de maíz existentes en el municipio de Molcaxac. La segunda parte tuvo como objetivo realizar una descripción general de las principales características de los productores de maíz, así como de su unidad...
Palavras-chave: Zea mays L.; Poblaciones locales; Diversidad genética; Sistmemas de producción; Local populations; Genetic diversity; Production systems; Maestría; Estrategías para el Desarrollo Agrícola Regional; EDAR.
Ano: 2010
URL: http://hdl.handle.net/10521/177

Diversidad genética y potencial de poblaciones criollas de maíz del centro - sur de Tamaulipas, México
Provedor de dados: 32
Autores: Pecina Martínez, José Agapito.
Se ha demostrado el potencial que tienen las poblaciones criollas de maíz de Tamaulipas;  sin embargo, éstas no se han aprovechado eficientemente, debido al escaso trabajo de  investigación, especialmente en mejoramiento genético realizado en el Estado. Por lo tanto,  es necesario preservar y explorar aún más la diversidad genética y seguir generando  conocimiento que permita definir estrategias y acciones para evitar su erosión y su posible  contaminación con maíces transgénicos que se introduzcan al país. Con base en lo anterior,  desde el 2001, en la Unidad Académica Multidisciplinaria de Agronomía y Ciencias de la  Universidad Autónoma de Tamaulipas se implementó un programa de rescate, conservación  y manejo de germoplasma criollo de maíz. De más de...
Palavras-chave: Maíz criollo de Tamaulipas; Diversidad genética; Aprovechamiento de la diversidad; Mejoramiento de maíz; Indicadores de respuesta ambiental. landraces of maize from Tamaulipas; Genetic diversity; Use of the diversity; Maize breeding; Environmental response indicators.
Ano: 2012
URL: http://hdl.handle.net/10521/823

Valoración del mejoramiento genético participativo in situ en poblaciones de maíz (Zea mays L.) criollo en el sureste del Estado de México.
Provedor de dados: 32
Autores: Zambrano Zambrano, Eddie Ely.
La selección de semilla es un proceso ancestral y cotidiano en el cultivo de poblaciones de maíz nativo (criollo); se ha planteado que mediante el fortalecimiento de ese proceso con elementos técnicos del mejoramiento genético se puede conservar la diversidad genética del maíz y acrecentar el potencial productivo. De aplicarse de manera generalizada, se impactaría en el minifundio y en más del 80% de la superficie cultivada con maíz en México (8.5 millones de hectáreas). En el área de Genética del Colegio de Postgraduados se ha practicado mejoramiento participativo desde 1995, mediante selección masal in situ en poblaciones de maíz nativo con agricultores del sureste del Estado de México. En las localidades de Montecillo y Ayapango, Estado de México,...
Palavras-chave: Zea mays; Riqueza genética; Mejoramiento participativo; Respuesta a la selección; Polimorfismo isoenzimático; Genetic diversity; Participatory genetic improvement; Selection response; Isoenzyme polimorphism; Genética; Maestría.
Ano: 2013
URL: http://hdl.handle.net/10521/1863

Diversidad genética y potencial de poblaciones criollas de maíz del centro - sur de Tamaulipas, México
Provedor de dados: 32
Autores: Pecina Martínez, José Agapito.
Se ha demostrado el potencial que tienen las poblaciones criollas de maíz de Tamaulipas;  sin embargo, éstas no se han aprovechado eficientemente, debido al escaso trabajo de  investigación, especialmente en mejoramiento genético realizado en el Estado. Por lo tanto,  es necesario preservar y explorar aún más la diversidad genética y seguir generando  conocimiento que permita definir estrategias y acciones para evitar su erosión y su posible  contaminación con maíces transgénicos que se introduzcan al país. Con base en lo anterior,  desde el 2001, en la Unidad Académica Multidisciplinaria de Agronomía y Ciencias de la  Universidad Autónoma de Tamaulipas se implementó un programa de rescate, conservación  y manejo de germoplasma criollo de maíz. De más de...
Tipo: Tesis
Palavras-chave: Maíz criollo de Tamaulipas; Diversidad genética; Aprovechamiento de la diversidad; Mejoramiento de maíz; Indicadores de respuesta ambiental.; Landraces of maize from Tamaulipas; Genetic diversity; Use of the diversity; Maize breeding; Environmental response indicators.
Ano: 2008
URL: http://hdl.handle.net/10521/1192

Caracterización clásica y molecular del huitlacoche [Ustilago maydis D.C. (Corda)], hongo de importancia social y económica en la región central de México.
Provedor de dados: 32
Autores: Calderón Fernández, María Leticia.
Las secuencias de ADNr han sido útiles para clasificar a muchos basidiomicetos, debido a que se pueden caracterizar en muchos niveles taxonómicos en base a sus regiones conservadas y variables. Tal es el caso de los Ustilaginales, los cuales tienen importancia por sus implicaciones económicas adversas, por lo que han sido estudiados mediante algunas de estas técnicas, especialmente con la región ITS del ADNr, ya que muestran diferencias a nivel de nucleótidos, permitiendo establecer la diversidad genética de hongos a nivel inter e intra-específico. En este contexto se tiene a Ustilago maydis, el cual es un hongo que infecta al maíz produciendo el “huitlacoche” o “carbón del maíz” del cual existen estudios limitados sobre su características morfológicas y...
Palavras-chave: Diversidad genética; Región ITS del ADNr; Ustilago maydis; Recursos genéticos; Genetic diversity; Huitlacoche; ITS-rDNA region; Genetic resources; Doctorado; EDAR; Estrategías para el Desarrollo Agrícola Regional.
Ano: 2010
URL: http://hdl.handle.net/10521/319

Huella genética de siete razas de maíz de Valles Altos de México.
Provedor de dados: 32
Autores: Rocandio Rodríguez, Mario.
Dentro de la diversidad del maíz (Zea mays L.) de México, uno de los grupos de mayor interés es el del complejo piramidal que se cultiva en Valles Altos centrales de México. Con la finalidad de caracterizar la diversidad genética de siete razas de maiz de Valles Altos, se realizó un estudio utilizando variables morfológicas y marcadores moleculares (SSRs). Se hizo, además, un análisis simultáneo de los datos disponibles sobre dos líneas de evidencia, para hacer clasificaciones más robustas. Se incluyeron entre 8 y 26 accesiones que han sido consideradas por diferentes autores como representativas de las razas Arrocillo Amarillo, Cónicos, Elotes Cónicos, Palomero Toluqueño, Chalqueño y Purépecha. La adquisición de semillas se realizó mediante solicitud a...
Palavras-chave: Zea mays L.; Diversidad genética; Marcadores moleculares; Teocintle; Análisis conjunto; Genetic diversity; Molecular markers; Teosinte; Joint analysis; Genética; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2092

Resistencia de clones de papa al tizón tardío (Phytophthora infestans) y su caracterización morfológica en el Valle de Toluca.
Provedor de dados: 32
Autores: Rivadeneira Ruales, Jorge Esteban.
El rendimiento, la resistencia a enfermedades y la calidad del tubérculo son las características más importantes en el mejoramiento genético de la papa (Solanum tuberosum); por ello, el objetivo de la presente investigación fue estudiar los componentes de varianza y heredabilidad en sentido estrecho (h2) de la respuesta a Phytophthora infestans, así como la caracterización morfológica de clones resistentes, así como seleccionar los clones con resistencia y buenas características agronómicas. Se evaluaron 198 clones en primera generación clonal de 20 cruzas, y 13 de los 20 progenitores en el campo experimental del Instituto Nacional de Investigaciones Forestales, Agrícolas y Pecuarias (INIFAP), en el valle de Toluca, Estado de México. Más del 30% de clones...
Palavras-chave: Solanum tuberosum; Phytophthora infestans; Resistencia; Tizón tardío; Heredabilidad; Diversidad genética; Clones; Cruzas; Progenitor; Resistance; Late blight; Heritability; Genetic diversity; Clones; Crosses; Parent; Maestría; Genética.
Ano: 2011
URL: http://hdl.handle.net/10521/505

Tracking the Genetic Effects of Global Warming: Drosophila and Other Model Systems
Provedor de dados: 7
Autores: Scheiner, Samuel M; National Science Foundation; sscheine@nsf.gov.
Current efforts to study the biological effects of global change have focused on ecological responses, particularly shifts in species ranges. Mostly ignored are microevolutionary changes. Genetic changes may be at least as important as ecological ones in determining species' responses. In addition, such changes may be a sensitive indicator of global changes that will provide different information than that provided by range shifts. We discuss potential candidate systems to use in such monitoring programs. Studies of Drosophila subobscura suggest that its chromosomal inversion polymorphisms are responding to global warming. Drosophila inversion polymorphisms can be useful indicators of the effects of climate change on populations and ecosystems. Other...
Tipo: Peer-Reviewed Reports
Palavras-chave: Biodiversity conservation; Biological indicator; Chromosomal inversion polymorphism; Climate change; Drosophila; Evolutionary ecology; Genetic diversity; Global warming; Microevolution.
Ano: 1998

Repetitive element palindromic PCR (rep-PCR) as a genetic tool to study interspecific diversity in Euphorbiaceae family
Provedor de dados: 69
Autores: Rampadarath,Sillma; Puchooa,Daneshwar; Bal,Subhasisa.
Background The classification of diversity in germplasm collections is important for plant breeding. The repetitive element palindromic-polymerase chain reaction (rep-PCR) technique was used to investigate inter-specific diversity within 17 species from the Euphorbiaceae family using REP and BOX primers. Results The agglomerative cluster analysis was used to evaluate the scoring data. BOX and REP gave amplification with polymorphism of 98.84% and 100% respectively. REP marker demarcated between the subgenus peltatae. Both markers confirmed Jatropha tanjorensis as a natural hybrid between Jatropha gossypifolia and Jatropha curcas. Five random sequences from the rep-PCR gels were chosen, cloned and sequenced. The blast results demonstrated that the amplified...
Tipo: Journal article
Palavras-chave: Euphorbiaceae; Genetic diversity; Jatropha; Mitochondrial genome; Rep-PCR.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000600005

Application of microsatellite markers for breeding and genetic conservation of herds of Pantaneiro sheep
Provedor de dados: 69
Autores: Crispim,Bruno do Amaral; Seno,Leonardo de Oliveira; Egito,Andréa Alves do; Vargas Junior,Fernando Miranda de; Grisolia,Alexéia Barufatti.
Background The aim of the present study was to assess the genetic diversity of Pantaneiro sheep, using microsatellite markers, in order to assist maintenance and management plans, enhance mating systems and reduce the inbreeding rate. A total of 127 animals were genotyped at eight microsatellite loci. They belonged to populations from the Experimental Farm of the Universidade Federal da Grande Dourados (UFGD) (Dourados/MS/Brazil) and Embrapa Pantanal (Corumbá/MS/Brazil). Results The population of Pantaneiro sheep from the UFGD exhibited a high mean number of alleles (11.13) and allelic richness (10.66). The polymorphic information content was highly informative in the locus studied, resulting in a mean value of 0.71. Observed heterozygosity was lower than...
Tipo: Journal article
Palavras-chave: Animal genetic resources; Genetic diversity; Kinship; Locally adapted breed; STR.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000600010

Assessment of genetic diversity in sesame (Sesamum indicum L.) detected by Amplified Fragment Length Polymorphism markers
Provedor de dados: 69
Autores: Ali,Ghulam M; Yasumoto,Sirato; Seki-Katsuta,Masumi.
Sesame (Sesamum indicum L.) is one of the oldest oil crops and is widely cultivated in Asia and Africa. To determine the level of genetic diversity in relation to geographical origins and morphological characteristics, a total of 96 accessions have been collected from different parts of the world and were analyzed using AFLP techniques. Twenty-one primer pairs generated a total of 445 bands and among them 157 (35%) were polymorphic. Using UPGMA clustering analysis method based on the similarity coefficient, accessions were separated into two major groups. The first group mostly consists of Eastern Asian origin and another group consists of South Asian origin. Sub-clusters separated the accessions and form distinct diversity among groups. Considering the...
Tipo: Journal article
Palavras-chave: AFLP; Genetic diversity; Geographical origin; Morphology; Sesame.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000100002

Genetic differentiation between Cinta Senese and commercial pig breeds using microsatellite
Provedor de dados: 69
Autores: Scali,Monica; Vignani,Rita; Bigliazzi,Jacopo; Paolucci,Elisa; Bernini,Andrea; Spiga,Ottavia; Niccolai,Neri; Cresti,Mauro.
Background: Cinta Senese (CS) is an autochthonous Tuscan breed, which risked extinction since the ‘60s. Results: Monitoring the genetic variability of the actual population by use of DNA molecular markers is essential to address a correct breeding policy, finalized to obtain the race preservation and its fitness in the future. 17 SSRs autosomal markers and 1 associated to the X chromosome were used to genotype 86 individuals belonging to the CS and 12 belonging to two main white races Landrace (L), Large White (LW) and crosses between LW and L and L and CS widespread in Tuscany and used in the recent past to obtain hybrids with the CS. Conclusions: A dendrogram of similarity measures the relative genetic distance between individuals in the population. Data...
Tipo: Journal article
Palavras-chave: Cinta Senese; Genetic diversity; Genotyping; Microsatellites; Sus scrofa.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200001

Assessment of genetic structure in Mexican charolais herds using microsatellite markers
Provedor de dados: 69
Autores: Sifuentes-Rincón,Ana María; Puentes-Montiel,Herlinda; Parra-Bracamonte,Gaspar Manuel.
Knowledge of livestock genetic diversity is an essential step to respond to commercial demands and reach production objectives in different environments and production systems. The evaluation of animal genetic diversity is achieved by using molecular markers. Microsatellites are the most used markers for studies of this type. Eleven microsatellites were used to evaluate the genetic variation from three populations of Charolais cattle located in northeast Mexico. The studied populations exhibited a high allelic variability with a mean heterozygosity of 0.5. A moderate genetic differentiation between the Charolais populations (F ST = 0.079; P &lt; 0.001) was observed. This suggests subdivisions in Charolais breed established in Mexico, due to genetic...
Tipo: Journal article
Palavras-chave: Charolais; Genetic diversity; Microsatellite.
Ano: 2007
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582007000400002

Genetic diversity associated with agronomic traits using microsatellite markers in Pakistani rice landraces
Provedor de dados: 69
Autores: Pervaiz,Zahida Hassan; Rabbani,M. Ashiq; Khaliq,Ishtiaq; Pearce,Stephen R; Malik,Salman A.
Genetic diversity underlies the improvement of crops by plant breeding. Land races of rice (Oryza sativa L.) can contain some valuable alleles not common in modern germplasm. The aim here was to measure genetic diversity and its effect on agronomic traits among rice land-race genotypes grown in Pakistan. Diversity was measured using thirty-five microsatellite markers and seventy-five genotypes. Among the markers used a total of 142 alleles were detected at 32 polymorphic SSR loci, while three loci were monomorphic in Pakistani rice landraces. The number of alleles identified by each marker ranged from 2 to 13 with a mean of 4.4. Size differences between the smallest and largest alleles varied from 11bp to 71bp. Polymorphism information content ranged from...
Tipo: Journal article
Palavras-chave: Genetic diversity; Landraces; Microsatellite markers; Oryza sativa L.; Pakistan; Rice.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300004

Soft computing model on genetic diversity and pathotype differentiation of pathogens: A novel approach
Provedor de dados: 69
Autores: Gürüler,Hüseyin; Peker,Musa; Baysal,Ömür.
Background Identifying and validating biomarkers' scores of polymorphic bands are important for studies related to the molecular diversity of pathogens. Although these validations provide more relevant results, the experiments are very complex and time-consuming. Besides rapid identification of plant pathogens causing disease, assessing genetic diversity and pathotype formation using automated soft computing methods are advantageous in terms of following genetic variation of pathogens on plants. In the present study, artificial neural network (ANN) as a soft computing method was applied to classify plant pathogen types and fungicide susceptibilities using the presence/absence of certain sequence markers as predictive features. Results A plant pathogen,...
Tipo: Journal article
Palavras-chave: Computational biology; Genetic diversity; Molecular markers; Plant pathogens; Predictive information; Soft computing.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000500004

Assessment of genetic diversity in Venezuelan rice cultivars using simple sequence repeats markers
Provedor de dados: 69
Autores: Ghneim Herrera,Thaura; Posso Duque,Duina; Pérez Almeida,Iris; Torrealba Núñez,Gelis; Pieters,Alejandro J; Martinez,César P; Tohme,Joe M.
In Venezuela, pedigree analyses indicate that the rice varieties currently under cultivation are closely related. Effective breeding programs, based on knowledge of the genetic diversity of cultivars, are needed to broaden the genetic bases of rice germplasm in the country. In this study, we used a set of 48 simple-sequence-repeat (SSR) markers to assess the genetic diversity of 11 Venezuelan rice cultivars, released by the National Rice Breeding Program between 1978 and 2007. A total of 203 alleles were detected, the number of alleles (NA) per marker ranged from 2 to 9, with an average of 4.23. The average genic diversity (H) over all SSR loci for the 18 genotypes was 0.524, ranging from 0.105 to 0.815. Positive correlations were found between H at each...
Tipo: Journal article
Palavras-chave: DNA fingerprinting; Genetic diversity; Rice; SSR.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000500003

Inbreeding evidence in a traditional channel catfish (Ictalurus punctatus) hatchery in Mexico
Provedor de dados: 69
Autores: Parra-Bracamonte,Gaspar Manuel; Sifuentes-Rincón,Ana María; Rosa-Reyna,Xochitl Fabiola De la; Arellano-Vera,Williams; Sosa-Reyes,Brenda.
Background: Channel catfish are one of the most important aquaculture species raised for food purposes in Mexico. Two temporal samples were obtained from the largest channel catfish breeding hatchery in Mexico to identify changes in genetic diversity and inbreeding that are promoted by traditional hatchery management. Results: The genetic parameter analysis of 11 microsatellite loci showed no significant change in genetic diversity (p &amp;gt; 0.05). However, a significant heterozygosis deficiency was detected (p &lt; 0.001), and genetic structure analysis indicated moderate differentiation between the temporally divided populations (F ST = 0.08). A moderate level of inbreeding and a slight increase of the inbreeding coefficient from 0.23 to 0.27 were the...
Tipo: Journal article
Palavras-chave: Channel catfish; Effective population size; Genetic diversity; Inbreeding.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000600011

Genetic polymorphism of the &amp;#945;s1-casein locus in five populations of goats from Mexico
Provedor de dados: 69
Autores: Torres-Vázquez,José Antonio; Vázquez Flores,Felícitas; Montaldo,Hugo H; Ulloa-Arvizu,Raúl; Valencia Posadas,Mauricio; Gayosso Vázquez,Amanda; Alonso Morales,Rogelio Alejandro.
With the objective of estimating allele frequencies, and testing for population divergence for the CSN1S1 locus, genotypes of animals from five goat populations; Saanen (n = 97), Alpine (n = 81) Toggenburg (n = 92), local goats with external appearance similar to the Murciana-Granadina breed from Central Mexico (n = 26) and heterogeneous local animals denominated Mosaico Lagunero (n = 30), from Northern Mexico, were identified using PCR and Xmn1 PCR-RFLP methodology. For Saanen, Alpine and Toggenburg, the sum of E and F alleles had the largest frequencies (from 0.468 to 0.789), while for the groups local Murciana-Granadina and Mosaico Lagunero the sum of the most frequent allelic groups (A* and B*), were 0.385 and 0.533 respectively. Both local...
Tipo: Journal article
Palavras-chave: CSN1S1; Gene frequencies; Genetic diversity; Goat milk; PCR-RFLP.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300007

Assessment of genetic diversity among Pakistani wheat (Triticum aestivum L.) advanced breeding lines using RAPD and SDS-PAGE
Provedor de dados: 69
Autores: Ahmed,Muhammad Farooq; Iqbal,Muhammad; Masood,Muhammad Shahid; Rabbani,Malik Ashiq; Munir,Muhammad.
Genetic diversity was assessed among 32 advanced wheat breeding lines included in the National Uniform Wheat Yield Trials (2006-07) of Pakistan using molecular (DNA) and biochemical (SDS-PAGE) markers. Of the 72 RAPD primers used for initial screening, 15 were found polymorphic. A total of 140 bands (61.4% polymorphic) were generated by the 15 random decamer primers. Genetic similarity coefficients ranged from 0.81 to 0.94 for rainfed and from 0.70 to 0.93 for the normal seeding date group. Cluster analysis using the unweighted pair group method of arithmetic averages (UPGMA) clustered the 32 advanced wheat breeding lines into one major and three small groups. Maximum level of polymorphism (90%) was observed for the primer OPA-05. Lines N9 and N11 showed...
Tipo: Journal article
Palavras-chave: Genetic diversity; Molecular markers; Polymorphism; RAPD; SDS-PAGE; Wheat.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000300001

Genetic diversity of Brassica oleracea var. capitata gene bank accessions assessed by AFLP
Provedor de dados: 69
Autores: Faltusová,Zuzana; Kuera,Ladislav; Ovesná,Jaroslava.
The genetic diversity of 20 cabbage (Brassica oleracea var. capitata, including sub.var. alba and rubra) cultivars and landraces from the Gene bank of Crop Research Institute was estimated using amplified fragment length polymorphism (AFLP) marker technology. Two cultivars of Brassica pekinensis (syn. Brassica rapa var. pekinensis) were used as outliers in the study. Thirty AFLP primer combinations produced a total of 1084 fragments. A total of 806 fragments, 364 (45%) of them polymorphic, were found across 20 Brassica oleracea var. capitata accessions. The accessions were clustered into two main groups. Special subgroups, reflecting place of origin, were observed within these groups. Ten selective primer pairs were found to be most informative because...
Tipo: Journal article
Palavras-chave: Amplified fragment length polymorphism; Brassica oleracea var. capitata; Cabbage; Discriminatory power; Genetic diversity.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000300011

Cotton genetic diversity study by AFLP markers
Provedor de dados: 69
Autores: Murtaza,Naveed.
Amplified fragmentlength polymorphism (AFLP) markers have been used to ascertain the intensity of inherent diversity and relatedness in cotton (Gossypium spp.) plants. The effectiveness of this method to distinguish inter and intra specific difference in cotton could be handy in cultivar recognition and in marker assisted parental selection tool for plant breeders. Twenty cotton cultivars belonging to Gossypium hirsutum L., and G. arborium L. from the Pakistan and US origin were used for AFLP based genetic diversity estimates. The objective of this study was to assess the level of genetic variation among some cotton cultivars belonging to the old and new world species of cotton. Four EcoRI-MseI primer-pair combinations were used forthe AFLP analysis. The...
Tipo: Journal article
Palavras-chave: AFLP; Cluster analysis; Cotton; Genetic diversity; PCR.
Ano: 2006
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582006000400013

Efficiency of RAPD, ISSR, AFLP and ISTR markers for the detection of polymorphisms and genetic relationships in camote de cerro (Dioscorea spp.)
Provedor de dados: 69
Autores: Velasco-Ramírez,Ana Paulina; Torres-Morán,Martha Isabel; Molina-Moret,Sandy; Sánchez-González,José de Jesús; Santacruz-Ruvalcaba,Fernando.
Background At present, species known as camote de cerro (Dioscorea spp.) are found only in the wilderness in Mexico, but their populations are extremely depleted because they are indiscriminately collected, it is urgent to evaluate the conservation status of these plants in order to design conservation genetics programs. In this study, genetic diversity parameters along with cluster analysis based on Jaccard's coefficient were estimated with the objective to assess the efficiency of Random Amplified Polymorphic DNA (RAPD), Inter Simple Sequence Repeat (ISSR), Amplified Fragment Length Polymorphism (AFLP) and Inverse Sequence Tagged Repeat (ISTR) molecular DNA markers in the Dioscorea genus. Results The polymorphic information contents were quite similar...
Tipo: Journal article
Palavras-chave: Dioscoreaceae; Genetic diversity; Molecular markers; Tuber.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000200002

Microsatellite markers in candidate genes for wood properties and its application in functional diversity assessment in Eucalyptus globulus
Provedor de dados: 69
Autores: Acuña,Cintia V; Villalba,Pamela V; García,Martín; Pathauer,Pablo; Hopp,H. Esteban; Marcucci Poltri,Susana N.
Background: Functional genetic markers have important implications for genetic analysis by providing direct estimation of functional diversity. Although high throughput sequencing techniques for functional diversity analysis are being developed nowadays, the use of already well established variable markers present in candidate genes is still an interesting alternative for mapping purposes and functional diversity studies. SSR markers are routinely used in most plant and animal breeding programs for many species including Eucalyptus. SSR markers derived from candidate genes (SSR-CG) can be used effectively in co-segregation studies and marker-assisted diversity management. Results: In the present study, eight new non reported SSRs were identified in seven...
Tipo: Journal article
Palavras-chave: Functional markers; Genetic diversity; Lignin pathway; SSR; Wood density.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200002

Analysis of genetic diversity among Indian niger [Guizotia abyssinica (L. f.) Cass.] cultivars based on randomly amplified polymorphic DNA markers
Provedor de dados: 69
Autores: Nagella,Praveen; Hosakatte,Niranjana Murthy; Ravishankar,K.V.; Hahn,Eun-Joo; Paek,Kee-Yoeup.
Randomly amplified polymorphic DNA (RAPD) markers were used to estimate genetic diversity among 18 cultivars of niger from India. Total genomic DNA was extracted and subjected to RAPD analysis using 80 arbitrary 10-mer primers; 17 primers were selected, which yielded a total of 124 bands, 41.20% of them polymorphic. None of the primers produced unique banding pattern for each cultivar. RAPD data were used to calculate a Squared-Euclidean Distance matrix which revealed a minimum genetic distance between cultivars JNC-6 and N-48 and a maximum distance between IGP-76 and JN-30. Based on the distance matrix, a cluster analysis was done using a minimum variance algorithm. The dendrogram generated, based on Ward&amp;#8217;s method, grouped 18 niger cultivars...
Tipo: Journal article
Palavras-chave: Genetic diversity; Guizotia abyssinica; Molecular markers; RAPD.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000100014

Decline of genetic variability in a captive population of Pacific white shrimp Penaeus (Litopenaeus) vannamei using microsatellite and pedigree information
Provedor de dados: 69
Autores: Vela Avitúa,Sergio; Montaldo,Hugo H; Márquez Valdelamar,Laura; Campos Montes,Gabriel R; Castillo Juárez,Héctor.
Background: The objective of this study was to estimate the decline of genetic variability and the changes in effective population size in three shrimp populations. One was a wild population collected at several points in the Mexican Pacific Ocean. The other two populations were different generations (7 and 9) from a captive population selected for growth and survival. Microsatellite markers and pedigree were both used to assess genetic variability and effective population size. Results: Using 26 loci, both captive populations showed a decline in the expected heterozygosity (20%) and allelic diversity indices (48 to 91%) compared to the wild population (P &lt; 0.05). The studied captive populations did not differ significantly from each other regarding...
Tipo: Journal article
Palavras-chave: Effective population size; Genetic diversity; Heterozygosity; Microsatellites; Selection; Shrimp.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000400009

Characterization of the genetic structure of mango ginger (Curcuma amada Roxb.) from Myanmar in farm and genebank collection by the neutral and functional genomic markers
Provedor de dados: 69
Autores: Jatoi,Shakeel Ahmad; Kikuchi,Akira; Ahmad,Dawood; Watanabe,Kazuo N.
A preliminary characterization was undertaken to describe genetic structure of mango ginger (Curcuma amada) acquired from farmers and ex situ genebank in Myanmar using neutral (rice SSR based RAPDs) and functional genomic (P450 based analog) markers. The high polymorphism (&gt; 91%) depicted has displayed existence of genetic variability in the germplasm investigated. Large number of source-specific alleles (neutral-markers = 78, functional-markers = 63) was amplified which revealed that neutral regions of the mango ginger were more variable compared with the functional regions. The major fraction of the molecular variance (neutral-markers = 85%, functional-markers = 93%) was explained within germplasm acquisition sources and this tendency was also...
Tipo: Journal article
Palavras-chave: Curcuma amada; Genetic diversity; Mango-ginger; Myanmar; On-farm.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000600004

Phylogenetic relationships among species of the subsection Dendrophlomis Bentham
Provedor de dados: 69
Autores: Yuzbasioglu,Ertugrul; Dadandi,Mehmet Yasar.
This study used randomly amplified polymorphic DNA markers to determine genetic relationships among species of the subsection Dendrophlomis. Twenty accessions of the eleven Phlomis taxa were evaluated to determine genetic variability using fourteen ten mer primers selected from a 125 random oligonucleotide set. These 14 selected primers generated 85 RAPD bands that ranged in size from 200 to 1200 base pairs. Of the total bands, 88% (75) were polymorphic among the samples. Genetic distances among accessions were computed to produce a dendrogram based on UPGMA. Genetic distances ranged from 0.133 (between P. amanica and P. monocephala) to 0.494 (between P. chimerae and P. lunariifolia). The UPGMA tree based on distances has two major groups. The first...
Tipo: Journal article
Palavras-chave: Genetic diversity; Genetic relationships; Genetic variation; Phlomis species; RAPDs.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000400012

Genetic diversity analysis of Bemisia tabaci populations in Pakistan using RAPD markers
Provedor de dados: 69
Autores: Hameed,Saima; Hameed,Shahid; Sadia,Munazza; Malik,Salman A.
Background: The Bemisia tabaci is one of the most devastating pests of agricultural crops and ornamental plants worldwide. The genetic diversity and biotype status of the Bemisia tabaci in Pakistan was assessed by using random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR). A total 80 samples of B. tabaci collected from 14 districts of the Punjab province and 7 districts of the Sindh province were included. Results: All 10 primers screened in this study generated 151 scorable amplification products, of which 117 or 77% were polymorphic. Pairwise Nei and Li’s similarity had ranged from 0.25 to 0.88 among all individuals analyzed. Based on Nei and Li’s similarity coefficients Bemisia populations were grouped into 3 main clusters and clearly...
Tipo: Journal article
Palavras-chave: Asia II 1; Genetic diversity; UPGMA; Whitefly.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000600006

Assessment of genetic diversity and relationships among Osmanthus fragrans cultivars using AFLP markers
Provedor de dados: 69
Autores: Yuan,Wang Jun; Han,Yuan Ji; Dong,Mei Fang; Shang,Fu De.
This study was conducted to reveal genetic diversity among 100 Osmanthus fragrans cultivars using amplified fragment length polymorphism (AFLP) markers. Eight AFLP primer combinations produced a total of 443 polymorphic fragments with an average of 64 per primer combination. The percentage of polymorphic bands (86.81%), the resolving power (Rp) (32.71) and the PIC values (0.331) showed the efficiency of used primer combinations. The revealed AFLP makers were effective in distinguishing all the cultivars considered. Cluster analysis were performed to assess patterns of diversity among cultivars and showed the abundant genetic diversity. The overall distribution pattern of molecular variation suggested that 93.33% of the total genetic variance was within the...
Tipo: Journal article
Palavras-chave: AFLP marker; Cluster analysis; Genetic diversity; Osmanthus fragrans.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582011000100002

Efficiency of RAPD and ISSR markers system in accessing genetic variation of rice bean (Vigna umbellata) landraces
Provedor de dados: 69
Autores: Muthusamy,Saraladevi; Kanagarajan,Selvaraju; Ponnusamy,Shanmugasundaram.
Vigna umbellata (Thunb.) Ohwi and Ohashi commonly known as rice bean or climbing mountain bean is under-exploited tropical legume. Genetic variation between 10 landraces of rice bean was evaluated using random amplified polymorphic DNA (RAPD) and inter simple sequence repeats (ISSR) markers. Among these markers, RAPD primers generated 987 amplification products of which 719 were polymorphic and ISSR markers produced 479 amplification products, out of which 296 were polymorphic. RAPD fingerprinting detected more polymorphic loci (70.30%) than the ISSR fingerprinting (61.79%). Mean PIC (polymorphism information content) for each of these marker systems (0.243 for RAPD and 0.203 for ISSR) suggested that both the marker systems were equally effective in...
Tipo: Journal article
Palavras-chave: Genetic diversity; ISSRs; Landraces; Rice bean; RAPDs; Vigna umbellata.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582008000300004

Genetic diversity and population structure analysis of strawberry (Fragaria x ananassa Duch.) using SSR markers
Provedor de dados: 69
Autores: Yoon,Min-Young; Moe,Kyaw Thu; Kim,Dae-Young; Rho,Il-Rae; Kim,Su; Kim,Ki-Taek; Won,Mi-Kyoung; Chung,Jong-Wook; Park,Yong-Jin.
In total, 18 simple sequence repeat (SSR) markers were used to analyze the genetic diversity and population structure of 59 accessions of cultivated strawberry (Fragaria x ananassa Duch.) from Korea, Germany, United States, United Kingdom, and Japan. In total, 101 alleles were detected with an average of 5.6 per locus and 21 specific alleles were identified. Notably, one genotype (Blonoli from Germany) possessed a maximum of 10 different unique alleles specific to each genotype. The gene diversity varied from 0.027 (EMPaEKO1B) to 0.791 (CFACT110), with an average value of 0.509. PIC values ranged from 0.026 to 0.762 (average 0.454). A model-based structure analysis revealed the presence of two populations. The accessions that were clearly assigned to a...
Tipo: Journal article
Palavras-chave: Genetic diversity; Population structure; SSR markers; Strawberry.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000200006

Genetic diversity analysis in the section Caulorrhizae (genus Arachis) using microsatellite markers
Provedor de dados: 74
Autores: Palmieri,Darío A.; Bechara,Marcelo D.; Curi,Rogério A.; Monteiro,Jomar P.; Valente,Sérgio E.S.; Gimenes,Marcos A.; Lopes,Catalina R.
Diversity in 26 microsatellite loci from section Caulorrhizae germplasm was evaluated by using 33 accessions of A. pintoi Krapov. &amp;amp; W.C. Gregory and ten accessions of Arachis repens Handro. Twenty loci proved to be polymorphic and a total of 196 alleles were detected with an average of 9.8 alleles per locus. The variability found in those loci was greater than the variability found using morphological characters, seed storage proteins and RAPD markers previously used in this germplasm. The high potential of these markers to detect species-specific alleles and discriminate among accessions was demonstrated. The set of microsatellite primer pairs developed by our group for A. pintoi are useful molecular tools for evaluating Section Caulorrhizae...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Germplasm; Microsatellites; Molecular markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100019

Genetic diversity of Hypostomus ancistroides (Teleostei, Loricariidae) from an urban stream
Provedor de dados: 74
Autores: Sofia,Silvia H.; Galindo,Bruno A.; Paula,Francine M.; Sodré,Leda M.K.; Martinez,Cláudia B.R.
In this study, random amplified polymorphic DNA (RAPD) markers were applied to analyze the genetic diversity of samples of the Neotropical catfish Hypostomus ancistroides, collected from four sites (S1, S2, S3 and S4) along an urban stream in Southern Brazil. The 11 primers used in RAPD analysis amplified 147 loci, 76 (51.7%) of which were polymorphic. The proportions of polymorphic loci observed in the four samples were: 29.93% (S1), 31.97% (S2), 23.81% (S3) and 38.77% (S4). The average heterozygosity within sampling localities ranged from 0.1230 to 0.1526 and unbiased genetic distances ranged from 0.0253 to 0.0445. AMOVA partitioned 90.85% of the total variation within samples and 9.15% among samples. Excepting for the sample pair S1-S2 (phiST = 0.02784;...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Loricariid fish; Genetic diversity; RAPD; Siluriformes.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200027

Genetic structure and conservation of Mountain Lions in the South-Brazilian Atlantic Rain Forest
Provedor de dados: 74
Autores: Castilho,Camila S.; Marins-Sá,Luiz G.; Benedet,Rodrigo C.; Freitas,Thales R.O.
The Brazilian Atlantic Rain Forest, one of the most endangered ecosystems worldwide, is also among the most important hotspots as regards biodiversity. Through intensive logging, the initial area has been reduced to around 12% of its original size. In this study we investigated the genetic variability and structure of the mountain lion, Puma concolor. Using 18 microsatellite loci we analyzed evidence of allele dropout, null alleles and stuttering, calculated the number of allele/locus, PIC, observed and expected heterozygosity, linkage disequilibrium, Hardy-Weinberg equilibrium, F IS, effective population size and genetic structure (MICROCHECKER, CERVUS, GENEPOP, FSTAT, ARLEQUIN, ONESAMP, LDNe, PCAGEN, GENECLASS software),we also determine whether there...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Araucaria Forest; Atlantic Rain Forest; Conservation; Genetic diversity; Microsatellite.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100009

Assessment of genetic diversity in Brazilian barley using SSR markers
Provedor de dados: 74
Autores: Ferreira,Jéssica Rosset; Pereira,Jorge Fernando; Turchetto,Caroline; Minella,Euclydes; Consoli,Luciano; Delatorre,Carla Andréa.
Abstract Barley is a major cereal grown widely and used in several food products, beverage production and animal fodder. Genetic diversity is a key component in breeding programs. We have analyzed the genetic diversity of barley accessions using microsatellite markers. The accessions were composed of wild and domesticated barley representing genotypes from six countries and three breeding programs in Brazil. A total of 280 alleles were detected, 36 unique to Brazilian barley. The marker Bmag120 showed the greatest polymorphism information content (PIC), with the highest mean value found on chromosome three, and the lowest on chromosomes four and six. The wild accessions presented the highest diversity followed by the foreign genotypes. Genetic analysis was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Domesticated barley; Genetic diversity; Hordeum vulgare ssp. vulgare; Hordeum vulgare ssp. spontaneum; Microsatellite markers.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572016000100086

Clonal diversity and conservation genetics of the medicinal plant Carapichea ipecacuanha (Rubiaceae)
Provedor de dados: 74
Autores: Oliveira,Luiz Orlando de; Venturini,Bruna Afonso; Rossi,Ana Aparecida Bandini; Hastenreiter,Saulo Santos.
The roots of the understorey shrub Carapichea ipecacuanha (ipecac) have medicinal properties, and the uprooting of wild plants has supplied most of the world demand for this species. Although under severe population decline, C. ipecacuanha lacks legal protection. In the wild, the aerial stems of ipecac clump together to form clusters with well-defined borders. Cluster size may range from several to hundreds of aerial stems. To investigate the extent of clonality among aerial stems in ipecac clusters, we sampled 50 wild clusters (a total of 291 aerial stems) and screened them with 89 inter-simple sequence repeat (ISSR) markers. The 291 aerial stems were grouped into 42 putative clones. The clonal groups generally consisted of aerial stems from the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Clonal propagation; Genetic diversity; ISSR; Poaia; Psychotria ipecacuanha.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100016

PCR-RFLP analysis of non-coding regions of cpDNA in Araucaria angustifolia (Bert.) O. Kuntze
Provedor de dados: 74
Autores: Schlögl,Paulo Sérgio; Souza,Anete Pereira de; Nodari,Rubens Onofre.
The Araucaria angustifolia (Bert.) O. Kuntze, also named the "paraná pine" (‘pinheiro-do-Paraná’ in Portuguese), is a native conifer species naturally occurring in the Brazilian Tropical Atlantic Forest which in Brazil is mostly limited to the southern Brazilian states of Paraná, Santa Catarina and Rio Grande do Sul. Chloroplast DNA markers (cpDNA) are useful in populational genetic studies because of their low substitution rate and the uniparental transmission. The conservation of cpDNA genes between species has allowed the design of consensus chloroplast primers that have had a great impact on population genetics and phylogenetic studies. In this study we used the polymerase chain reaction technique combined with restriction enzyme fragment length...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Haplotypes; Conifer species; Brazilian pine; Genetic diversity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300020

Genetic diversity in a world germplasm collection of tall fescue
Provedor de dados: 74
Autores: Cuyeu,Romina; Rosso,Beatriz; Pagano,Elba; Soto,Gabriela; Fox,Romina; Ayub,Nicolás Daniel.
Festuca arundinacea Schreb., commonly known as tall fescue, is a major forage crop in temperate regions. Recently, a molecular analysis of different accessions of a world germplasm collection of tall fescue has demonstrated that it contains different species from the genus Festuca and allowed their rapid classification into the three major morphotypes (Continental, Mediterranean and Rhizomatous). In this study, we explored the genetic diversity of 161 accessions of Festuca species from 29 countries, including 28 accessions of INTA (Argentina), by analyzing 15 polymorphic SSR markers by capillary electrophoresis. These molecular markers allowed us to detect a total of 214 alleles. The number of alleles per locus varied between 5 and 24, and the values of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: SSR markers; Genetic diversity; Flow cytometry; Tall fescue; Genetic resources.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000200014

SSR and AFLP based genetic diversity of soybean germplasm differing in photoperiod sensitivity
Provedor de dados: 74
Autores: Singh,Ram K.; Bhatia,Virendra S.; Bhat,K.V.; Mohapatra,Trilochan; Singh,Nagendra K.; Bansal,Kailash C.; Koundal,K.R.
Forty-four soybean genotypes with different photoperiod response were selected after screening of 1000 soybean accessions under artificial condition and were profiled using 40 SSR and 5 AFLP primer pairs. The average polymorphism information content (PIC) for SSR and AFLP marker systems was 0.507 and 0.120, respectively. Clustering of genotypes was done using UPGMA method for SSR and AFLP and correlation was 0.337 and 0.504, respectively. Mantel's correlation coefficients between Jaccard's similarity coefficient and the cophenetic values were fairly high in both the marker systems (SSR = 0.924; AFLP = 0.958) indicating very good fit for the clustering pattern. UPGMA based cluster analysis classified soybean genotypes into four major groups with fairly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Photoperiod response; SSR; AFLP; Genetic diversity; Soybean.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200019

Genetic divergence of rubber tree estimated by multivariate techniques and microsatellite markers
Provedor de dados: 74
Autores: Gouvêa,Lígia Regina Lima; Rubiano,Luciana Benchimol; Chioratto,Alisson Fernando; Zucchi,Maria Imaculada; Gonçalves,Paulo de Souza.
Genetic diversity of 60 Hevea genotypes, consisting of Asiatic, Amazonian, African and IAC clones, and pertaining to the genetic breeding program of the Agronomic Institute (IAC), Brazil, was estimated. Analyses were based on phenotypic multivariate parameters and microsatellites. Five agronomic descriptors were employed in multivariate procedures, such as Standard Euclidian Distance, Tocher clustering and principal component analysis. Genetic variability among the genotypes was estimated with 68 selected polymorphic SSRs, by way of Modified Rogers Genetic Distance and UPGMA clustering. Structure software in a Bayesian approach was used in discriminating among groups. Genetic diversity was estimated through Nei's statistics. The genotypes were clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genealogy; Genetic diversity; Hevea brasiliensis; Multivariate analysis; SSRs.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200018

Genetic diversity in section Rhizomatosae of the genus Arachis (Fabaceae) based on microsatellite markers
Provedor de dados: 74
Autores: Angelici,Carla M.L.C.D.; Hoshino,Andrea Akemi; Nóbile,Paula Macedo; Palmieri,Dario Abel; Valls,José F. Montenegro; Gimenes,Marcos A.; Lopes,Catalina Romero.
The genus Arachis (Fabaceae) native to South America, contains 80 species divided into nine sections, three of which contain species of special economic importance such as the cultivated peanut (Arachis hypogaea), belonging to the section Arachis, and some perennial forage species from sections Caulorrhizae and Rhizomatosae. We used microsatellite markers to assay genetic variability among 77 accessions of four species from section Rhizomatosae, the diploid Arachis burkartii (2n = 2x = 20) and the tetraploid Arachis glabrata, Arachis pseudovillosa and Arachis nitida (2n = 4x = 40). A total of 249 alleles were found in the fifteen loci analyzed and a high degree of intra and interspecific polymorphism was detected. The lowest intraspecific variation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arachis; Genetic diversity; Microsatellite; Peanut; Rhizomatosae.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100016

Molecular characterization of beta-tubulin from Phakopsora pachyrhizi, the causal agent of Asian soybean rust
Provedor de dados: 74
Autores: Maciel,Talles Eduardo Ferreira; Freire,Maíra Cristina Menezes; Almeida,Álvaro M.R. de; Oliveira,Luiz Orlando de.
β-tubulins are structural components of microtubules and the targets of benzimidazole fungicides used to control many diseases of agricultural importance. Intron polymorphisms in the intron-rich genes of these proteins have been used in phylogeographic investigations of phytopathogenic fungi. In this work, we sequenced 2764 nucleotides of the β-tubulin gene (Pp tubB) in samples of Phakopsora pachyrhizi collected from seven soybean fields in Brazil. Pp tubB contained an open reading frame of 1341 nucleotides, including nine exons and eight introns. Exon length varied from 14 to 880 nucleotides, whereas intron length varied from 76 to 102 nucleotides. The presence of only four polymorphic sites limited the usefulness of Pp tubB for phylogeographic studies in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Asian soybean rust; Beta-tubulin; Genetic diversity; Phakopsora pachyrhizi; Soybeans.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200025

The genetics and conservation of Araucaria angustifolia: I. Genetic structure and diversity of natural populations by means of non-adaptive variation in the state of Santa Catarina, Brazil
Provedor de dados: 74
Autores: Auler,Neiva Maria Frizon; Reis,Maurício Sedrez dos; Guerra,Miguel Pedro; Nodari,Rubens Onofre.
The objective of this study was to generate information relative to the allele distribution and dynamics within and among populations of Araucaria angustifolia, a naturally-occurring conifer in the south of Brazil, being known popularly as "pinheiro-do-Paraná", "araucaria" or pine tree. In order to elucidate the levels and the distribution of the genetic variability, the population's genetic structure and the genetic distance among natural populations of this species with different levels of disturbance in different geographical areas were studied in detail. For this, samples of leaf tissue were collected from 328 adult individuals in nine natural populations in Santa Catarina State. To analyze the samples, the allozyme technique was applied in starch gel...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pinheiro; Forest degradation; Genetic diversity; Genetic erosion.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000300014

Analysis of genetic diversity and population structure in Argentine and Bolivian Creole cattle using five loci related to milk production
Provedor de dados: 74
Autores: Lirón,J.P.; Ripoli,M.V.; De Luca,J.C.; Peral-García,P.; Giovambattista,G.
Data from five protein-coding loci related to dairy production were used to study the genetic diversity and population structure of Argentine and Bolivian Creole cattle breeds. Genomic DNA was extracted from blood samples of six Creole cattle breeds: Argentine (n = 230), Patagonian (n = 25); "Saavedreño" (n = 140), "Chaqueño Boliviano" (n = 30), "Yacumeño" (n = 27), and "Chusco" (n = 11). kappa-casein, beta-lactoglobulin, growth hormone and prolactin were measured by PCR-RFLP, while alphaS1-casein was typed by PCR-ASO. The results are discussed, focusing on: historical origin, recent differentiation and selection events, Zebu gene introgression, and population structure. This work shows that: (i) For the studied genes, the observed gene frequency profiles...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Creole cattle; Polymorphism; Genetic diversity; Molecular markers.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400010

Genetics, evolution and conservation of Bromeliaceae
Provedor de dados: 74
Autores: Zanella,Camila M.; Janke,Aline; Palma-Silva,Clarisse; Kaltchuk-Santos,Eliane; Pinheiro,Felipe G.; Paggi,Gecele M.; Soares,Luis E.S.; Goetze,Márcia; Büttow,Miriam V.; Bered,Fernanda.
Bromeliaceae is a morphologically distinctive and ecologically diverse family originating in the New World. Three centers of diversity, 58 genera, and about 3,140 bromeliad species are currently recognized. We compiled all of the studies related to the reproductive biology, genetic diversity, and population structure of the Bromeliaceae, and discuss the evolution and conservation of this family. Bromeliads are preferentially pollinated by vertebrates and show marked variation in breeding systems, from predominant inbreeding to predominant outcrossing, as well as constancy in chromosome number (2n = 2x = 50). Autogamous or mixed mating system bromeliads have a high inbreeding coefficient (F IS), while outcrossing species show low F IS. The degree of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bromeliads; Cytogenetics; Genetic diversity; Population structure; Reproductive biology.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000600017

Fluorescent amplified fragment length polymorphism (fAFLP) analyses and genetic diversity in Litopenaeus vannamei (Penaeidae)
Provedor de dados: 74
Autores: Gonçalves,Michelle Mantovani; Lemos,Manoel Victor Franco; Galetti Junior,Pedro Manoel; Freitas,Patrícia Domingues de; Furtado Neto,Manuel Antonio Andrade.
The Pacific white shrimp, Litopenaeus vannamei (Penaeidae), represents about 95% of all Brazilian shrimp production. The Brazilian L. vannamei foundation broodstock was made up of specimens collected from different American Pacific sites, but little information was collected on the genetic structure of the broodstock. We used the fluorescence amplified fragment length polymorphism (fAFLP) method to study the genetic diversity of L. vannamei broodstock lines 03CMF1 and 03CBF1 originally produced by breeder-shrimps imported mainly from Panama and Ecuador, although wild individuals from other localities may also have been used in producing these two lines. Our results showed a total of 93 polymorphic bands ranging from 50 to 500 bp, the mean Nei's genetic...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: FAFLP; Genetic diversity; Litopenaeus vannamei; Penaeidae; Shrimp.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000200016

Green turtles (Chelonia mydas) foraging at Arvoredo Island in Southern Brazil: genetic characterization and mixed stock analysis through mtDNA control region haplotypes
Provedor de dados: 74
Autores: Proietti,Maíra Carneiro; Lara-Ruiz,Paula; Reisser,Júlia Wiener; Pinto,Luciano da Silva; Dellagostin,Odir Antonio; Marins,Luis Fernando.
We analyzed mtDNA control region sequences of green turtles (Chelonia mydas) from Arvoredo Island, a foraging ground in southern Brazil, and identified eight haplotypes. Of these, CM-A8 (64%) and CM-A5 (22%) were dominant, the remainder presenting low frequencies (&lt; 5%). Haplotype (h) and nucleotide ( π) diversities were 0.5570 ± 0.0697 and 0.0021 ± 0.0016, respectively. Exact tests of differentiation and AMOVA ΦST pairwise values between the study area and eight other Atlantic foraging grounds revealed significant differences in most areas, except Ubatuba and Rocas/Noronha, in Brazil (p &gt; 0.05). Mixed Stock Analysis, incorporating eleven Atlantic and one Mediterranean rookery as possible sources of individuals, indicated Ascension and Aves islands...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Foraging grounds; Genetic diversity; Green turtle; MtDNA haplotypes; Natal origins.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300027

Genetic polymorphisms at the leptin receptor gene in three beef cattle breeds
Provedor de dados: 74
Autores: Almeida,Sabrina E.M.; Santos,Luciana B.S.; Passos,Daniel T.; Corbellini,Ângela O.; Lopes,Beatriz M.T.; Kirst,Cláudia; Terra,Gustavo; Neves,Jairo P.; Gonçalves,Paulo B.D.; Moraes,José C.F.; Weimer,Tania de Azevedo.
The genetic diversity of a single nucleotide polymorphism (SNP) at the exon 20 (T945M) of the leptin receptor gene (LEPR) and of three short tandem repeats (STRs BM7225, BMS694, and BMS2145) linked to LEPR was investigated in three beef cattle herds (Brangus Ibagé, Charolais, and Aberdeen Angus). A cheap and effective new method to analyze the T945M polymorphism in cattle populations was developed and the possible role of these polymorphisms in reproduction and weight gain of postpartum cows was evaluated. High levels of genetic diversity were observed with the average heterozygosity of STRs ranging from 0.71 to 0.81. No significant association was detected between LEPR markers and reproductive parameters or daily weight gain. These negative results...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Cattle production; LEPR gene polymorphisms.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000400013

Mitochondrial control region genetic diversity and maternal ancestry of a Brangus-Ibage cattle populations
Provedor de dados: 74
Autores: Henkes,Luiz Ernani; Silva Jr,Wilson Araújo; Moraes,José Carlos Ferrugem; Weimer,Tania Azevedo.
The genetic diversity of 277 nucleotides in the mitochondrial DNA control region (nt 15,964 to 16,240 in reference sequence) was analyzed in crossbreed beef cattle (Brangus-Ibage, 5/8 Bos primigenius taurus x 3/8 Bos primigenius indicus) as well as in some Nellore samples (B. p. indicus). Fifty-seven mutations were found in Brangus-Ibage comprising 18 haplotypes (haplotype diversity, h = 0.851 ± 0.041 and nucleotide diversity, ntd = 0.009 ± 0.006) and 66 in Nellore (h = 1.00 ± 0.27, ntd = 0.014 ± 0.012). These data indicated sequence identities of 99.6 and 92.1% between the B. p. taurus' reference sequence and Brangus-Ibage and Nellore, respectively. The comparison of our data with sequence data for 612 individuals recovered from GenBank showed a total of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine mtDNA; Maternal lineage; Sequence analysis; Beef cattle; Genetic diversity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572005000100011

Genetic diversity and phylogenetic relationships in the rye genus Secale L. (rye) based on Secale cereale microsatellite markers
Provedor de dados: 74
Autores: Shang,Hai-Ying; Wei,Yu-Ming; Wang,Xiao-Rong; Zheng,You-Liang.
The genetic diversity and phylogenetic relationships in the genus Secale L. (rye) was evaluated using 24 Secale cereale microsatellite (SCM) markers. The average polymorphism information content (PIC) value of each microsatellite locus in 30 Secale accessions evaluated was higher than that in 47 cultivated ryes (Secale cereale ssp. cereale). The mean genetic similarity (GS) index in Secale was lower than that in cultivated rye. The highest within-species GS index was observed for S. sylvestre and the lowest for S. strictum, whereas the highest between-species GS index was found between S. cereale and S. vavilovii and the lowest between S. sylvestre and S. cereale. There was no obvious difference in GS levels in the cultivated rye accessions from Asia,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cultivated rye; Genetic diversity; Microsatellite markers; Phylogenetic relationships; Secale L.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000400018

Genetic diversity between herds of Alpine and Saanen dairy goats and the naturalized Brazilian Moxotó breed
Provedor de dados: 74
Autores: Araújo,Adriana Mello de; Guimarães,Simone Eliza Facioni; Machado,Thea Mírian Medeiros; Lopes,Paulo Sávio; Pereira,Carmen Silva; Silva,Francisco Luiz Ribeiro da; Rodrigues,Marcelo Teixeira; Columbiano,Virgínia de Souza; Fonseca,Cleusa Graça da.
Brazilian naturalized goat breeds are adapted to the semiarid conditions prevalent in the Northeast region of the country (which has the largest Brazilian goat heard) and represent an as yet uninvestigated source of genetic diversity. Currently, imported goat breeds are crossed with Brazilian naturalized goat breeds, endangering the genetic potential of the naturalized breeds. We used 11 microsatellite markers to determine the genetic diversity among imported (non-naturalized) dairy Alpine and Saanen goats and naturalized Brazilian Moxotó goats. We genotyped 292 goats from three herds (one private, one from the University of Minas Gerais and the Moxotó conservation herd from Embrapa Caprinos) and found that the general heterozygosity was 0.6952 for Alpine,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; DNA microsatellite; Genetic distance; Genetic diversity; Goats.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000100014

Diversity analysis of Bemisia tabaci biotypes: RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region
Provedor de dados: 74
Autores: Rabello,Aline R.; Queiroz,Paulo R.; Simões,Kenya C.C.; Hiragi,Cássia O.; Lima,Luzia H.C.; Oliveira,Maria Regina V.; Mehta,Angela.
The Bemisia tabaci complex is formed by approximately 41 biotypes, two of which (B and BR) occur in Brazil. In this work we aimed at obtaining genetic markers to assess the genetic diversity of the different biotypes. In order to do that we analyzed Bemisia tabaci biotypes B, BR, Q and Cassava using molecular techniques including RAPD, PCR-RFLP and sequencing of the ITS1 rDNA region. The analyses revealed a high similarity between the individuals of the B and Q biotypes, which could be distinguished from the BR individuals. A phylogenetic tree based on ITS1 rDNA sequence was constructed. This is the first report of the ITS1 rDNA sequence of Bemisia tuberculata and of the BR biotype of B. tabaci.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Whitefly; ITS rDNA; Genetic diversity; Phylogenetic analysis.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000300029

Informative microsatellites for genetic population studies of black-faced lion tamarins (Leontopithecus caissara)
Provedor de dados: 74
Autores: Martins,Milene Moura; Galetti Junior,Pedro Manoel.
Leontopithecus caissara is a critically endangered primate species from the Brazilian Atlantic Forest. Nineteen microsatellite loci, previously developed for congeneric species, were tested with 34 L. caissara individuals from Superagüi Island. Of the 19 loci, 17 (89.4%) produced robust alleles, nine (47.4%) of these proved to be polymorphic, with a total of 23 alleles and an average of 2.56 alleles per locus. Expected and observed heterozygosity averaged 0.483 and 0.561, respectively. The exclusion power for identifying the first parent of an arbitrary offspring was 0.315 over all loci. The results thus indicate both the usefulness and limitations of these nine microsatellite loci in the genetic analysis of L. caissara, as well as their potentiality for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Lion tamarins; Endangered species; Genetic diversity; New World primate; SSR transferability.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000100030

Genetic variation in the Solanaceae fruit bearing species lulo and tree tomato revealed by Conserved Ortholog (COSII) markers
Provedor de dados: 74
Autores: Enciso-Rodríguez,Felix; Martínez,Rodrigo; Lobo,Mario; Barrero,Luz Stella.
The Lulo or naranjilla (Solanum quitoense Lam.) and the tree tomato or tamarillo (Solanum betaceum Cav. Sendt.) are both Andean tropical fruit species with high nutritional value and the potential for becoming premium products in local and export markets. Herein, we present a report on the genetic characterization of 62 accessions of lulos (n = 32) and tree tomatoes (n = 30) through the use of PCR-based markers developed from single-copy conserved orthologous genes (COSII) in other Solanaceae (Asterid) species. We successfully PCR amplified a set of these markers for lulos (34 out of 46 initially tested) and tree tomatoes (26 out of 41) for molecular studies. Six polymorphic COSII markers were found in lulo with a total of 47 alleles and five polymorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: COSII; Genetic diversity; Lulo; Tree tomato.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000200013

Genetic structure of Brazilian wild rice (Oryza glumaepatula Steud., Poaceae) populations analyzed using microsatellite markers
Provedor de dados: 74
Autores: Karasawa,Marines M.G.; Vencovsky,Roland; Silva,Cynthia M.; Zucchi,Maria Imaculada; Oliveira,Giancarlo C.X.; Veasey,Elizabeth A.
Knowledge of the genetic structure and diversity of natural populations is important in developing strategies for in situ and ex situ conservation. We used eight microsatellite loci to estimate genetic structure and investigate within and between population genetic variation in eleven Brazilian wild rice (Oryza glumaepatula) populations. The study showed the following genetic diversity parameters: average number of 3.1 alleles per locus; 77.3% polymorphic loci; 0.091 observed heterozygosity and 0.393 gene diversity. F-statistics detected by microsatellite loci were: F ST = 0.491 (and R ST = 0.608), F IS = 0.780 and F IT = 0.888. No population was in Hardy-Weinberg equilibrium. The estimated apparent outcrossing rate (0.143) indicated a predominance of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Gene flow; Genetic diversity; Microsatellites; Oryza glumaepatula.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300017

Genetic variability and efficiency of DNA microsatellite markers for paternity testing in horse breeds from the Brazilian Marajó archipelago
Provedor de dados: 74
Autores: Reis,Sávio P.; Gonçalves,Evonnildo C.; Silva,Artur; Schneider,Maria P.C.
In this study, 15 microsatellite DNA loci used in comparative tests by the International Society for Animal Genetics were applied to the evaluation of genetic diversity and management, and the efficiency of paternity testing in Marajoara horses and Puruca ponies from the Marajó Archipelago. Based on the genotyping of 93 animals, mean allelic diversity was estimated as 9.14 and 7.00 for the Marajoara and Puruca breeds, respectively. While these values are similar to those recorded in most European breeds, mean levels of heterozygosity were much lower (Marajoara 49%, Puruca 40%), probably as a result of high levels of inbreeding in the Marajó populations. The mean informative polymorphic content of this 15-marker system was over 50% in both breeds, and was...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Marajoara horses; Puruca ponies; Microsatellite markers; Genetic diversity; Geneaological control.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000100014

Using genetic diversity information to establish core collections of Stylosanthes capitata and Stylosanthes macrocephala
Provedor de dados: 74
Autores: Santos-Garcia,Melissa Oliveira; Toledo-Silva,Guilherme de; Sassaki,Rodrigo Possidonio; Ferreira,Thais Helena; Resende,Rosângela Maria Simeão; Chiari,Lucimara; Karia,Cláudio Takao; Carvalho,Marcelo Ayres; Faleiro,Fábio Gelape; Zucchi,Maria Imaculada; Souza,Anete Pereira de.
Stylosanthes species are important forage legumes in tropical and subtropical areas. S. macrocephala and S. capitata germplasm collections that consist of 134 and 192 accessions, respectively, are maintained at the Brazilian Agricultural Research Corporation Cerrados (Embrapa-Cerrados). Polymorphic microsatellite markers were used to assess genetic diversity and population structure with the aim to assemble a core collection. The mean values of H O and H E for S. macrocephala were 0.08 and 0.36, respectively, whereas the means for S. capitata were 0.48 and 0.50, respectively. Roger's genetic distance varied from 0 to 0.83 for S. macrocephala and from 0 to 0.85 for S. capitata. Analysis with STRUCTURE software distinguished five groups among the S....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Stylosanthes; Tropical forage; Microsatellites; Genetic diversity; Core collection.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500020

Characterization of six microsatellite loci in  Myrica faya  (Myricaceae)   and cross amplification in the endangered endemic  M. rivas-martinezii    in Canary Islands, Spain
Provedor de dados: 74
Autores: González-Pérez,Miguel A.; Newton,Craig; Sosa,Pedro A.; Rivero,Elizabeth; González-González,Edna A.
Six novel polymorphic microsatellite markers were isolated from enriched libraries in  Myrica faya  Ait., recently renamed  Morella faya , (fayatree, firetree, or firebush) in order to examine the genetic diversity in natural populations. Also, test cross-specific amplification and genetic diversity in  Myrica rivas-martinezii,  which is endemic on the Canary islands. Microsatellite loci were screened in 225 individuals of both species from different islands of the Canarian archipelago. All markers were successfully amplified from both  Myrica  species, with an average number of 6.5 and 9.3 alleles per locus in  M. rivas-martinezii  and  M. faya , respectively. There was no evidence for linkage disequilibrium between loci, and the probability of null...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrica rivas-martinezii; Myrica faya; Microsatellite; Canary Islands; Genetic diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100017

AFLP marker analysis revealing genetic structure of the tree Parapiptadenia rigida (Benth.) Brenan (Leguminosae-Mimosoideae) in the southern Brazilian Tropical Rainforest
Provedor de dados: 74
Autores: Souza,Laís Bérgamo de; Ruas,Eduardo A.; Rodrigues,Luana A.; Ruas,Claudete F.; Ruas,Paulo M.
Parapiptadenia rigida is a tropical early secondary succession tree characteristic of the Tropical Atlantic Rainforest. This species is of great ecological importance in the recovery of degraded areas. In this study we investigated the variability and population genetic structure of eight populations of P. rigida. Five AFLP primer combinations were used in a sample of 159 individuals representing these eight populations, rendering a total of 126 polymorphic fragments. The averages of percentage of polymorphic loci, gene diversity, and Shannon index were 60.45%, 0.217, and 0.322, respectively. A significant correlation between the population genetic variability and the population sizes was observed. The genetic variability within populations (72.20%) was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tropical tree; Genetic diversity; Population genetics; Conservation; AFLP.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000400012

Determination of genetic variation in Rhodiola crenulata from the Hengduan Mountains Region, China using inter-simple sequence repeats
Provedor de dados: 74
Autores: Lei,Yidong; Gao,Hong; Tsering,Tashi; Shi,Suhua; Zhong,Yang.
The plant Rhodiola crenulata is a perennial herbaceous species distributed in the plateau region of southwestern China, especially the Hengduan Mountains region. It has been one of the most important traditional herbal remedies in Tibet for more than one thousand years, but the accelerated and uncontrolled collection of this plant since the 1980s has lead to deforestation. We used inter-simple sequence repeats (ISSR) to assess levels of genetic variation in R. crenulata from nine diverse natural populations in eastern Tibet and northern Yunnan, the first time such a study has been carried out. The 12 primers we used were able to detect 184 polymorphic loc. Analysis of molecular variance (AMOVA) indicated that species level genetic diversity was relatively...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Hengduan Mountains region; ISSR; Rhodiola crenulata.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200023

Ecological drivers of plant genetic diversity at the southern edge of geographical distributions: Forestal vines in a temperate region
Provedor de dados: 74
Autores: Barros,Michel J.F.; Diniz-Filho,José Alexandre F.; Freitas,Loreta B.
Abstract The Tropical Niche Conservatism hypothesis is one of the most relevant theories to explain why tropical diversity is high, although the mechanisms underlying this hypothesis require further clarification. A possible research avenue to address the underlying mechanisms includes determining population-level processes associated with such a hypothesis, in particular by trying to identify how adaptation may occur in extreme niche conditions at the edges of species ranges. However, the determinants of molecular diversity at the edges of geographical distributions of tropical taxa are still poorly known. Here we assessed which environmental variables determine diversity in nuclear and plastid genetic markers for populations of four Passiflora species in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Distribution edges; Genetic diversity; Passiflora; Niche conservatism; Environmental drivers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000200318

Microsatellite analysis of a sample of Uruguayan Creole bulls (Bos taurus)
Provedor de dados: 74
Autores: Armstrong,Eileen; Postiglioni,Alicia; Martínez,Amparo; Rincón,Gonzalo; Vega-Pla,José Luis.
The Uruguayan Creole cattle genetic reserve consists of a herd of about 600 animals (bulls, cows and calves) located in an indigenous habitat of 650 hectares. In a previous study, a random sample from this herd showed high heterozygosity and a Hardy-Weinberg equilibrium for markers of major genes related to milk production. To study its genetic diversity we genotyped a sample of bulls (N = 19 out of 23 for the whole herd) using the PCR reaction with a set of 17 microsatellite markers. Between two and seven different alleles were identified per microsatellite in a total of 73 alleles. The expected mean heterozygosity (He) per locus was between 0.465 and 0.801, except for microsatellite HEL13 which gave a He value of 0.288. The expected mean heterozygosity...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Uruguayan Creole cattle; Genetic diversity; Microsatellites.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200012

Exploiting a wheat EST database to assess genetic diversity
Provedor de dados: 74
Autores: Karakas,Ozge; Gurel,Filiz; Uncuoglu,Ahu Altinkut.
Expressed sequence tag (EST) markers have been used to assess variety and genetic diversity in wheat (Triticum aestivum). In this study, 1549 ESTs from wheat infested with yellow rust were used to examine the genetic diversity of six susceptible and resistant wheat cultivars. The aim of using these cultivars was to improve the competitiveness of public wheat breeding programs through the intensive use of modern, particularly marker-assisted, selection technologies. The F2 individuals derived from cultivar crosses were screened for resistance to yellow rust at the seedling stage in greenhouses and adult stage in the field to identify DNA markers genetically linked to resistance. Five hundred and sixty ESTs were assembled into 136 contigs and 989 singletons....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Biodiversity; EST; Genetic diversity; Triticum; Yellow rust.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400019

DNA sampling from eggshells and microsatellite genotyping in rare tropical birds: Case study on Brazilian Merganser
Provedor de dados: 74
Autores: Maia,Thais Augusta; Vilaça,Sibelle Torres; Silva,Luciana Resende da; Santos,Fabricio Rodrigues; Dantas,Gisele Pires de Mendonça.
Abstract This study shows that sampling maternal DNA from hatched and abandoned eggshells is a viable noninvasive strategy for studying the genetics of rare or endangered tropical birds, as exemplified here by the Brazilian Merganser (Mergus octosetaceus). Eighteen microsatellites were isolated from enriched libraries and nine heterologous loci from related species were tested. Seven loci were amplified successfully, with five of them being polymorphic. These loci exhibited amplicons ranging from 110 to 254 bp for 132 samples, with 60 from eggshells and 72 from blood or muscle samples. The number of alleles for M. octosetaceus ranged from one to six (mean = 3.71), which is low compared to M. merganser (1-15 alleles), a ‘least concern’ species. Genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Noninvasive samples; Genetic diversity; Endangered birds; Mergus.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000500808

Genetic structure of Argentinean hexaploid wheat germplasm
Provedor de dados: 74
Autores: Vanzetti,Leonardo S.; Yerkovich,Nadia; Chialvo,Eugenia; Lombardo,Lucio; Vaschetto,Luis; Helguera,Marcelo.
The identification of genetically homogeneous groups of individuals is an ancient issue in population genetics and in the case of crops like wheat, it can be valuable information for breeding programs, genetic mapping and germplasm resources. In this work we determined the genetic structure of a set of 102 Argentinean bread wheat (Triticum aestivum L.) elite cultivars using 38 biochemical and molecular markers (functional, closely linked to genes and neutral ones) distributed throughout 18 wheat chromosomes. Genetic relationships among these lines were examined using model-based clustering methods. In the analysis three subpopulations were identified which correspond largely to the origin of the germplasm used by the main breeding programs in Argentina.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; Genetic structure; Molecular markers; Genetic diversity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572013000300014

Transference of microsatellite markers from Eucalyptus spp to Acca sellowiana and the successful use of this technique in genetic characterization
Provedor de dados: 74
Autores: Santos,Karine Louise dos; Welter,Leocir José; Dantas,Adriana Cibele de Mesquita; Guerra,Miguel Pedro; Ducroquet,Jean Pierre Henri Joseph; Nodari,Rubens Onofre.
The pineapple guava (Acca sellowiana), known in portuguese as the goiabeira-serrana or "Feijoa", is a native fruit tree from southern Brazil and northern Uruguay that has commercial potential due to the quality and unique flavor of its fruits. Knowledge of genetic variability is an important tool in various steps of a breeding program, which can be facilitated by the use of molecular markers. The conservation of repeated sequences among related species permits the transferability of microsatellite markers from Eucalyptus spp. to A. sellowiana for testing. We used primers developed for Eucalyptus to characterize A. sellowiana accessions. Out of 404 primers tested, 180 amplified visible products and 38 were polymorphic. A total of 48 alleles were detected...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Feijoa sellowiana; Genetic diversity; Goiabeira-serrana; Pineapple-guava; Transferability.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100014

The use and limits of ITS data in the analysis of intraspecific variation in Passiflora L. (Passifloraceae)
Provedor de dados: 74
Autores: Mäder,Geraldo; Zamberlan,Priscilla M.; Fagundes,Nelson J.R.; Magnus,Tielli; Salzano,Francisco M.; Bonatto,Sandro L.; Freitas,Loreta B.
The discovery and characterization of informative intraspecific genetic markers is fundamental for evolutionary and conservation genetics studies. Here, we used nuclear ribosomal ITS sequences to access intraspecific genetic diversity in 23 species of the genus Passiflora L. Some degree of variation was detected in 21 of these. The Passiflora and Decaloba (DC.) Rchb. subgenera showed significant differences in the sizes of the two ITS regions and in GC content, which can be related to reproductive characteristics of species in these subgenera. Furthermore, clear geographical patterns in the spatial distribution of sequence types were identified in six species. The results indicate that ITS may be a useful tool for the evaluation of intraspecific genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Intraspecific variability; ITS; Passiflora; Phylogeography.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000100018

Intraspecific genetic diversity of Drechslera tritici-repentis as detected by random amplified polymorphic DNA analysis
Provedor de dados: 74
Autores: Santos,Ana Maria Pujol Vieira dos; Matsumura,Aida T. Santos; Van Der Sand,Sueli Teresinha.
The phytopathogenic fungus Drechslera tritici-repentis causes tan spot, an important disease of wheat in the southern Brazilian state of Rio Grande do Sul. Twelve D. tritici-repentis isolates were obtained from wheat seeds from different locations in the state. Their colony morphology on potato dextrose agar and polymorphisms in genomic DNA by the random amplified polymorphic DNA (RAPD) method were investigated. For the RAPD method, 23 primers were tested of which nine were selected for use in the study of D. tritici-repentis polymorphisms. The degree of similarity between isolates was calculated using a simple matching coefficient and dendrograms constructed by the unweighted pair-group method with arithmetical averages (UPGMA). The morphological and RAPD...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Drechslera tritici-repentis; RAPD; DNA; Polymorphism; Genetic diversity.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000200020

Population structure and genetic diversity of the giant anteater (Myrmecophaga tridactyla: Myrmecophagidae, Pilosa) in Brazil
Provedor de dados: 74
Autores: Clozato,Camila L.; Miranda,Flávia R.; Lara-Ruiz,Paula; Collevatti,Rosane G.; Santos,Fabrício R.
Abstract The giant anteater (Myrmecophaga tridactyla, Pilosa, Linnaeus 1758) belongs to the mammalian order Pilosa and presents a large distribution along South America, occupying a great variety of habitats. It is listed in the IUCN Red List of threatened species as Vulnerable. Despite threatened, there is a lack of studies regarding its genetic variability. The aim of this study was to examine the genetic diversity and patterns of genetic structure within remaining populations. We analyzed 77 individuals from seven different populations distributed in four biomes across Brazil: Cerrado, Pantanal, Atlantic Forest and Amazon Forest. We sequenced two mitochondrial markers (control region and Cyt-b) and two nuclear markers (AMELY and RAG2). We found high...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Giant Anteater; Xenarthra; Cerrado; Genetic diversity; Population structure.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572017000100050

Population genetic analysis of Caiman crocodilus (Linnaeus, 1758) from South America
Provedor de dados: 74
Autores: Vasconcelos,William R.; Hrbek,Tomas; Silveira,Ronis da; Thoisy,Benoit de; Marioni,Boris; Farias,Izeni P.
The genetic structure of Caiman crocodilus was investigated using a 1085 bp mtDNA fragment of the cytochrome b gene. Inferences were based on 125 individuals from nine localities in Peru, Brazil and French Guiana. With the exception of Mamirauá Lake, Anavilhanas Archipelago and the Tapará Community which show a signal of demographic expansion, the sampled localities are in a mutation-drift genetic equilibrium. Divergence between the Amazon basin and extra-Amazon basin localities is significant; however, inference from Nested Clade Analysis cannot distinguish between continuous range expansion, long distance colonization or past fragmentation; however, past fragmentation is unlikely due to low number of mutational steps separating these two regions. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic structure; Phylogeography; Genetic diversity; Demographic expansion; Cytochrome b; Caiman crocodilus.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200006

Intraspecific differentiation of Chilean isolates of the entomopathogenic fungi Metarhizium anisopliae var. anisopliae as revealed by RAPD, SSR and ITS markers
Provedor de dados: 74
Autores: Becerra Velásquez,Viviana; Paredes Cárcamo,Mario; Rojo Meriño,Carmen; France Iglesias,Andrés; Franco Durán,Jorge.
The genus Metarhizium consists of a diverse group of asexual entomopathogenic fungi, which have a wide geographical distribution. The Chilean National Agricultural Research Institute (Instituto de Investigaciones Agropecuarias - INIA, Quilamapu Chile) has collected about 350 isolates of Metarhizium anisopliae var. anisopliae from central and southern Chile. These isolates have been partially characterized using morphological traits such as conidia size and shape, colony color, growth pattern and the efficiency of the isolates in controlling specific pests. However, further characterization with molecular markers could detect differences in DNA which could help to better understand the genetic diversity and structure of Chilean populations of this fungus....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Metarhizium; Genetic diversity; RAPD; SSR; ITS markers.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000100017

Genetic characterization of cassava ( Manihot esculenta ) landraces   in Brazil assessed with simple sequence repeats
Provedor de dados: 74
Autores: Siqueira,Marcos V. B. M.; Queiroz-Silva,Jurema R.; Bressan,Eduardo A.; Borges,Aline; Pereira,Kayo J. C.; Pinto,José G.; Veasey,Elizabeth Ann.
Based on nine microsatellite loci, the aim of this study was to appraise the genetic diversity of 42 cassava ( Manihot esculenta ) landraces from selected regions in Brazil, and examine how this variety is distributed according to origin in several municipalities in the states of Minas Gerais, São Paulo, Mato Grosso do Sul, Amazonas and Mato Grosso. High diversity values were found among the five above-mentioned regions, with 3.3 alleles per locus on an average, a high percentage of polymorphic loci varying from 88.8% to 100%, an average of 0.265 for observed heterozygosity and 0.570 for gene diversity. Most genetic diversity was concentrated within the regions themselves ( H   S   = 0.52). Cluster analysis and principal component based scatter plotting...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; SSR markers; Traditional farming.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000100015

Developing a common bean core collection suitable for association mapping studies
Provedor de dados: 74
Autores: Perseguini,Juliana Morini Küpper Cardoso; Silva,Gliciane Micaele Borges; Rosa,João Ricardo Bachega Feijó; Gazaffi,Rodrigo; Marçal,Jéssica Fernanda; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando; Zucchi,Maria Imaculada; Garcia,Antonio Augusto Franco; Benchimol-Reis,Luciana Lasry.
Because of the continuous introduction of germplasm from abroad, some collections have a high number of accessions, making it difficult to explore the genetic variability present in a germplasm bank for conservation and breeding purposes. Therefore, the aim of this study was to quantify and analyze the structure of genetic variability among 500 common bean accessions to construct a core collection. A total of 58 SSRs were used for this purpose. The polymorphism information content (PIC) in the 180 common bean accessions selected to compose the core collection ranged from 0.17 to 0.86, and the discriminatory power (DP) ranged from 0.21 to 0.90. The 500 accessions were clustered into 15 distinct groups and the 180 accessions into four distinct groups in the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular markers; Genetic diversity; Genetic structure; Microsatellites; Phaseolus vulgaris L.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572015000100067

Structure and genetic relationships between Brazilian naturalized and exotic purebred goat domestic goat (Capra hircus) breeds based on microsatellites
Provedor de dados: 74
Autores: Oliveira,Joelliton Domingos de; Igarashi,Maria Luiza Silveira de Paiva; Machado,Théa Mírian Medeiros; Miretti,Marcos Mateo; Ferro,Jesus Aparecido; Contel,Eucleia Primo Betioli.
The genetic relationships and structure of fourteen goat (Capra hircus) populations were estimated based on genotyping data from 14 goat populations (n = 410 goats) at 13 microsatellite loci. We used analysis of molecular variance (AMOVA), principal component analysis (PCA) and F statistics (F IS, F IT and F ST) to evaluate the genetic diversity (Ho, He and ad) of the goats. Genetic distances between the 14 goat populations were calculated from allelic frequency data for the 13 microsatellite markers. Moderate differentiation was observed for the populations of the undefined breeds (including the Anglo-Nubian-M breed), the naturalized Brazilian breeds (Moxotó, Canindé), the exotic purebred breeds (Alpine, Saanen, Toggenbourg and Anglo-Nubian) and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Goat; Microsatellite; Genetic distance; PCA; AMOVA; Genetic relationships; Genetic diversity; F statistics.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000300010

Polymorphic heterologous microsatellite loci for population genetics studies of the white-faced ibis Plegadis chihi (Vieillot, 1817) (Pelecaniformes, Threskiornithidae)
Provedor de dados: 74
Autores: Souza,Andiara Silos Moraes de Castro e; Miño,Carolina Isabel; Lama,Silvia Nassif Del.
We screened 44 heterologous microsatellites isolated in species of the families Threskiornithidae, Ciconiidae and Ardeidae for their use in a migratory waterbird, the white-faced ibis Plegadis chihi (Vieillot, 1817) (Threskiornithidae). Of the screened loci, 57% amplified successfully and 24% were polymorphic. In two breeding colonies from southern Brazil (N = 131) we detected 32 alleles (2-10 alleles/ locus). Average He over all loci and colonies was 0.55, and the combined probability of excluding false parents, 98%. There was no departure from HWE in any loci or population. Eru6 and Eru4 loci were in non-random association in the Alvorada colony, and NnNF5 and Eru5 in both populations. AMOVA analysis indicated that most of the genetic diversity was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-species amplification; Genetic diversity; Molecular markers; White-faced ibis; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000100010

Genetic diversity analysis of Cuban traditional rice (Oryza sativa L.) varieties based on microsatellite markers
Provedor de dados: 74
Autores: Alvarez,Alba; Fuentes,Jorge Luis; Puldón,Violeta; Gómez,Pedro Julio; Mora,Leonor; Duque,Miriam C.; Gallego,Gerardo; Tohme,Joe M.
Microsatellite polymorphism was studied in a sample of 39 traditional rice (Oryza sativa L.) varieties and 11 improved varieties widely planted in Cuba. The study was aimed at assessing the extent of genetic variation in traditional and improved varieties and to establish their genetic relationship for breeding purposes. Heterozygosity was analyzed at each microsatellite loci and for each genotype using 10 microsatellite primer pairs. Between varieties genetic relationship was estimated. The number of alleles per microsatellite loci was 4 to 8, averaging 6.6 alleles per locus. Higher heterozygosity (H) was found in traditional varieties (H TV = 0.72) than in improved varieties (H IV = 0.42), and 68% of the total microsatellite alleles were found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellite; Rice; Traditional germplasm.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572007000600014

Genetic relationships among native americans based on beta-globin gene cluster haplotype frequencies
Provedor de dados: 74
Autores: Mousinho-Ribeiro,Rita de Cassia; Pante-de-Sousa,Gabriella; Santos,Eduardo José Melo dos; Guerreiro,João Farias.
The distribution of b-globin gene haplotypes was studied in 209 Amerindians from eight tribes of the Brazilian Amazon: Asurini from Xingú, Awá-Guajá, Parakanã, Urubú-Kaapór, Zoé, Kayapó (Xikrin from the Bacajá village), Katuena, and Tiriyó. Nine different haplotypes were found, two of which (n. 11 and 13) had not been previously identified in Brazilian indigenous populations. Haplotype 2 (+ - - - -) was the most common in all groups studied, with frequencies varying from 70% to 100%, followed by haplotype 6 (- + + - +), with frequencies between 7% and 18%. The frequency distribution of the b-globin gene haplotypes in the eighteen Brazilian Amerindian populations studied to date is characterized by a reduced number of haplotypes (average of 3.5) and low...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA polymorphisms; Brazilian Amerindians; Genetic diversity.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572003000300002

Transferability and use of microsatellite markers for the genetic analysis of the germplasm of some Arachis section species of the genus Arachis
Provedor de dados: 74
Autores: Bravo,Juliana Pereira; Hoshino,Andrea Akemi; Angelici,Carla Maria Lara C.D.; Lopes,Catalina Romero; Gimenes,Marcos Aparecido.
The Arachis section is the most important of the nine sections of the genus Arachis because it includes the cultivated peanut, Arachis hypogaea. The genetic improvement of A. hypogaea using wild relatives is at an early stage of development in spite of their potential as sources of genes, including those for disease and pests resistance, that are not found in the A. hypogaea primary gene pool. Section Arachis species germplasm has been collected and maintained in gene banks and its use and effective conservation depends on our knowledge of the genetic variability contained in this material. Microsatellites are routinely used for the analysis of genetic variability because they are highly polymorphic and codominant. The objective of this study was to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Germplasm; Peanut; Transferability; Wild species.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000300021

Genetic differentiation and geographical Relationship of Asian barley landraces using SSRs
Provedor de dados: 74
Autores: Naeem,Rehan; Dahleen,Lynn; Mirza,Bushra.
Genetic diversity in 403 morphologically distinct landraces of barley (Hordeum vulgare L. subsp. vulgare) originating from seven geographical zones of Asia was studied using simple sequence repeat (SSR) markers from regions of medium to high recombination in the barley genome. The seven polymorphic SSR markers representing each of the chromosomes chosen for the study revealed a high level of allelic diversity among the landraces. Genetic richness was highest in those from India, followed by Pakistan while it was lowest for Uzbekistan and Turkmenistan. Out of the 50 alleles detected, 15 were unique to a geographic region. Genetic diversity was highest for landraces from Pakistan (0.70 ± 0.06) and lowest for those from Uzbekistan (0.18 ± 0.17). Likewise,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster analysis; Genetic diversity; Geographical differentiation; Hordeum vulgare; Principal component analysis.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000200017

Nucleotide diversity and molecular evolution of the WAG-2 gene in common wheat (Triticum aestivum L) and its relatives
Provedor de dados: 74
Autores: Wei,Shuhong; Peng,Zhengsong; Zhou,Yonghong; Yang,Zaijun; Wu,Kai; Ouyang,Zhongming.
In this work, we examined the genetic diversity and evolution of the WAG-2 gene based on new WAG-2 alleles isolated from wheat and its relatives. Only single nucleotide polymorphisms (SNP) and no insertions and deletions (indels) were found in exon sequences of WAG-2 from different species. More SNPs and indels occurred in introns than in exons. For exons, exons+introns and introns, the nucleotide polymorphism Π decreased from diploid and tetraploid genotypes to hexaploid genotypes. This finding indicated that the diversity of WAG-2 in diploids was greater than in hexaploids because of the strong selection pressure on the latter. All dn/ds ratios were &lt; 1.0, indicating that WAG-2 belongs to a conserved gene affected by negative selection. Thirty-nine of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Copy number variation; Genetic diversity; Molecular evolution; Triticum; WAG-2.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572011000400013

Change in genetic size of small-closed populations: lessons from a domestic mammal population
Provedor de dados: 74
Autores: Ghafouri-Kesbi,Farhad.
The aim of this study was to monitor changes in genetic size of a small-closed population of Iranian Zandi sheep, by using pedigree information from animals born between 1991 and 2005. The genetic size was assessed by using measures based on the probability of identity-by-descend of genes (coancestry, f, and effective population size, Ne), as well as measures based on probability of gene origin (effective number of founders, f e, effective number of founder genomes, f g, and effective number of non-founder genomes, f ne). Average coancestry, or the degree of genetic similarity of individuals, increased from 0.81% to 1.44% during the period 1993 to 2005, at the same time that Ne decreased from 263 to 93. The observed trend for f e was irregular throughout...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pedigree; Effective size; Genetic drift; Genetic diversity; Genetic similarity; Sheep.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400011

PCR-based VNTR core sequence analysis for inferring genetic diversity in the shrimp Litopenaeus vannamei
Provedor de dados: 74
Autores: Freitas,Patrícia Domingues de; Galetti Junior,Pedro Manoel.
The genetic variation in two farmed strains (F3-Panama and F17-Venezuela) of the shrimp Litopenaeus vannamei was examined based on DNA multiloci analyses. Eighteen adults of each strain were analyzed by PCR using a set of VNTR core sequence primers. Genetic similarity, mean allele frequency, mean heterozygosity and the frequency of polymorphic loci were determined for both strains. A dendrogram of genetic similarity was produced by UPGMA clustering. The results for three primers (INS, M13, YN73) revealed different levels of genetic variation within the strains. The higher genetic similarity seen within strain F17 was apparently related to inbreeding, although a bottleneck effect could not be discarded. The low level of genetic variability of this strain...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Genetic diversity; Shrimp; VNTR; PCR.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572002000400012

Population structures of Brazilian Tall coconut (Cocos nucifera L.) by microsatellite markers
Provedor de dados: 74
Autores: Ribeiro,Francisco E.; Baudouin,Luc; Lebrun,Patricia; Chaves,Lázaro J.; Brondani,Claudio; Zucchi,Maria I.; Vencovsky,Roland.
Coconut palms of the Tall group were introduced to Brazil from the Cape Verde Islands in 1553. The present study sought to evaluate the genetic diversity among and within Brazilian Tall coconut populations. Samples were collected of 195 trees from 10 populations. Genetic diversity was accessed by investigating 13 simple sequence repeats (SSR) loci. This provided a total of 68 alleles, ranging from 2 to 13 alleles per locus, with an average of 5.23. The mean values of gene diversity (He) and observed heterozygosity (Ho) were 0.459 and 0.443, respectively. The genetic differentiation among populations was estimated at θ = 0.1600 and the estimated apparent outcrossing rate was t a = 0.92. Estimates of genetic distances between the populations varied from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coconut; Genetic diversity; Genetic variability; Molecular markers; SSR.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572010000400016

Genetic diversity and geographical differentiation of cultivated six-rowed naked barley landraces from the Qinghai-Tibet plateau of China detected by SSR analysis
Provedor de dados: 74
Autores: Feng,Zong-Yun; Zhang,Li-Li; Zhang,Yi-Zheng; Ling,Hong-Qing.
Cultivated six-rowed naked barley (Hordeum vulgare ssp. hexastichon var. nudum Hsü) is the oldest cultivated barley in China. We used 35 simple sequence repeat (SSR) markers selected from seven barley linkage groups to study the genetic diversity, geographical differentiation and evolutionary relationships among 65 H. vulgare ssp. hexastichon landrace accessions collected from the Qinghai-Tibet plateau of China, 25 accessions from Tibet (TB), 20 from Qinghai (QH) and 20 from Ganzi (GZ) prefecture in Sichuan province. At the 35 SSR loci we identified 248 alleles among the 65 accessions, 119 (47.98%) of the alleles being common alleles. We also found that the TB accessions possessed 47 private alleles, about 1.5 times more than the 31 private alleles found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Barley; Genetic diversity; Qinghai-Tibet plateau; Geographical differentiation; Simple sequence repeat marker.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572006000200022

Combinations of distance measures and clustering algorithms in pepper germplasm characterization
Provedor de dados: 75
Autores: Gomes,Gisely Paula; Baba,Viviane Yumi; Santos,Odair P dos; Sudré,Cláudia P; Bento,Cintia dos S; Rodrigues,Rosana; Gonçalves,Leandro SA.
ABSTRACT Characterization and evaluation of genotypes conserved in the germplasm banks have become of great importance due to gradual loss of genetic variability and search for more adapted and productive genotypes. This can be obtained through several ways, generating quantitative and qualitative data. Joint analysis of those variables may be considered a strategy for an accurate germplasm characterization. In this study we aimed to evaluate different clustering techniques for characterization and evaluation of Capsicum spp. accessions using combinations of specific measures for quantitative and qualitative variables. A collection of 56 Capsicum spp. accessions was characterized based on 25 morphoagronomic descriptors. Six quantitative distances were used...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Capsicum spp.; Multivariate analysis; Clustering methods; Genetic diversity; Qualitative and quantitative descriptors.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362019000200172

Molecular characterization of potato cultivars using SSR markers
Provedor de dados: 75
Autores: Favoretto,Patrícia; Veasey,Elizabeth Ann; Melo,Paulo César Tavares de.
The potato crop has a very narrow genetic base, so the use of molecular markers is a very important tool in the characterization of germplasm banks and evaluation of genetic divergence. The objective of this study was to identify, using microsatellite or simple sequence repeat (SSR) markers, 38 accessions of potato from two collections of commercial cultivars. For the molecular characterization 10 loci were used, generating a total of 46 alleles, which were analyzed as binary data. A cluster analysis was performed with the Jaccard´s similarity coefficient and the UPGMA method, using the software NTSYSpc. On average, the number of alleles per locus was 4.6, ranging from two alleles for primers STM1049, STM 1053 and STM 1104 to 12 alleles per locus for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Solanum tuberosum; Germplasm banks; Genetic diversity; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362011000400017

DNA fingerprinting of water yam (Dioscorea alata) cultivars in Brazil based on microsatellite markers
Provedor de dados: 75
Autores: Siqueira,Marcos VBM; Dequigiovanni,Gabriel; Corazon-Guivin,Mike A; Feltran,José C; Veasey,Elizabeth A.
This study aimed to fingerprint 36 water yam (Dioscorea alata) accessions using microsatellite markers. Ten accessions were collected in local markets from several municipalities in Brazil, eight were obtained from the 'Instituto Agronômico de Campinas' (IAC) germplasm collection and eighteen were collected directly from growers from São Paulo state. A total of nine microsatellite loci were used in the analysis. Loci revealed high polymorphism verified by elevated PIC values (0.57-0.77), and by high gene diversity and Shannon-Wiener indices (0.69 and 1.29 on average, respectively). The accessions were classified into two groups based on clustering analysis. One group contained mostly accessions from the IAC collection, including a commercial cultivar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscorea alata; Commercial varieties; Genetic diversity; Germplasm collection; SSR.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000400015

Genetic resources of vegetable crops: a survey in the Brazilian germplasm collections pictured through papers published in the journals of the Brazilian Society for Horticultural Science
Provedor de dados: 75
Autores: Sudré,Cláudia P; Leonardecz,Eduardo; Rodrigues,Rosana; Amaral Júnior,Antônio T do; Moura,Maria da CL; Gonçalves,Leandro SA.
The research on plant genetic resources is essential for the conservation of genetic diversity and accessions' divergence studies, as a basis for plant breeding. Aiming to know the state of art in this subject, a historical survey was carried out in Revista de Olericultura and in Horticultura Brasileira, from 1961 to 2006, searching for papers dealing with vegetable crops genetic resources. In each of the papers, the species studied, first author institution, publication year, applied software, number of accessions and descriptors, and the multivariate techniques used were registered. Based on these characteristics, papers were grouped using multivariate analysis. Sixty-one papers dealt somehow with genetic resources in the time covered by the survey, from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Review; Multivariate analysis; Diagnosis; Genetic diversity; Vegetables.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362007000400002

Microsatellite genotyping from faeces of Lontra longicaudis from southern Brazil
Provedor de dados: 76
Autores: Weber,Laura I.; Hildebrand,Cintia G.; Ferreira,Anderson; Pedarassi,Gustavo; Levy,José A.; Colares,Elton P.
A genetic study of the neotropical river otter Lontra longicaudis (Olfers, 1818), which has an unknown conservation status, was carried out at the Taim Ecological Station and the margins of the Vargas stream, Rio Grande do Sul, southern Brazil. Faecal samples were collected, and DNA was extracted using a silica-guanidine method. Five microsatellite loci were amplified using PCR with heterologous primers previously described for Lutra lutra (Linnaeus, 1758). Sixteen faecal samples out of 29 from Taim and 11 out of 14 from Vargas stream margins contained enough DNA for genetic analysis. A total of 49 different alleles were found at both localities, from which 18 were exclusively found in individuals from Taim and 17 were exclusives from Vargas individuals....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neotropical river otter; Genetic markers; Taim; Non-invasive sampling; Genetic diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0073-47212009000100001

Conséquences génétiques de la production de larves d'huîtres en écloserie : étude des processus de dérive et de sélection
Provedor de dados: 5
Autores: Taris, Nicolas; Sauvage, Christopher; Batista, Frederico; Baron, Sophie; Ernande, Bruno; Haffray, Pierrick; Boudry, Pierre.
Previous studies have shown heritable variation in larval developmental traits in the Pacific oyster Crassostrea gigas. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors, specific to hatcheries, were examined: the effect of discarding the smallest larvae (i.e. culling) and the effect of temperature (20°C versus 26°C). A mixed-family approach was used in order to infer the genetic composition of larval populations and family assignment, limiting possible environmental bias and allowing the study of a relatively large number of families using a limited number of larval tanks. Our results show that three multiplexed highly polymorphic microsatellite markers are a powerful tool for family assignment and,...
Tipo: Text
Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-1505.pdf

Genetic diversity of the harmful family Kareniaceae (Gymnodiniales, Dinophyceae) in France, with the description of Karlodinium gentienii sp. nov.: A new potentially toxic dinoflagellate
Provedor de dados: 5
Autores: Nezan, Elisabeth; Siano, Raffaele; Boulben, Sylviane; Six, Christophe; Bilien, Gwenael; Cheze, Karine; Duval, Audrey; Le Panse, Sophie; Quere, Julien; Chomerat, Nicolas.
The family Kareniaceae is mostly known in France for recurrent blooms of Karenia mikimotoi in the Atlantic, English Channel, and Mediterranean Sea and for the unusual green discoloration in the saltwater lagoon of Diana (Corsica) caused by Karlodinium corsicum in April 1994. In terms of diversity, this taxonomic group was long overlooked owing to the difficult identification of these small unarmored dinoflagellates. In this study, thanks to the molecular characterization performed on single cells from field samples and cultures, twelve taxonomic units were assigned to the known genera Karenia, Karlodinium and Takayama, whereas one could not be affiliated to any described genus. The molecular phylogeny inferred from the D1–D2 region of the LSU rDNA showed...
Tipo: Text
Palavras-chave: Genetic diversity; Haemolytic; Kareniaceae; Pigments; Single cell; Taxonomy.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00225/33611/32310.pdf

Estimates of genetic variability and inbreeding in experimentally selected populations of European sea bass
Provedor de dados: 5
Autores: Hillen, J. E. J.; Coscia, I.; Vandeputte, Marc; Herten, K.; Hellemans, B.; Maroso, F.; Vergnet, Alain; Allal, Francois; Maes, G. E.; Volckaert, F. A. M.
The aquaculture industry has increasingly aimed at improving economically important traits like growth, feed efficiency and resistance to infections. Artificial selection represents an important window of opportunity to significantly improve production. However, the pitfall is that selection will reduce genetic diversity and increase inbreeding in the farmed stocks. Genetic tools are very useful in this context as they provide accurate measures of genetic diversity together with many additional insights in the stock status and the selection process. In this study we assessed the level of genetic variability and relatedness over several generations of two lines of experimentally selected European sea bass (Dicentrarchus labrax L.). The first line was...
Tipo: Text
Palavras-chave: Artificial selection; DdRAD; Fish; Genetic diversity; Genomics; Inbreeding.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00392/50314/50993.pdf

Conséquences génétiques de la production intensive de larves d'huîtres en écloserie : étude des processus de dérive et de sélection liés aux pratiques d'élevage.
Provedor de dados: 5
Autores: Taris, Nicolas.
In order to study the genetic influence of intensive production of the larval stage in commercial hatcheries, two types of specific selective processes were examined: the effects of discarding the smallest larvae and temperature. A mixed-family approach was used in order to infer the genetic composition of the larvae.  The results show that our mixed-family approach, combined with microsatellite-based family assignment, is a powerful tool for the study of bivalve larvae genetics. Selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss. The culled larvae are important for the minimization of the variability in reproductive success and the maximization of broodstock genetic diversity....
Tipo: Text
Palavras-chave: Hatchery; Oyster; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Huître; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-976.pdf

Phenotypic and genetic consequences of size selection at the larval stage in the Pacific oyster (Crassostrea gigas)
Provedor de dados: 5
Autores: Taris, Nicolas; Ernande, Bruno; Mccombie, Helen; Boudry, Pierre.
The life histories of oysters in the genus Crassostrea, like those of most marine bivalves, are typified by high fecundity and low survival in nature. Rearing conditions in hatcheries however ensure optimized density, diet, and temperature. Hatcheries are becoming increasingly important for the production of juveniles in aquaculture, and their culture practices often include culling of slow growing larvae to reduce and synchronize the time taken to reach settlement. Because previous studies have found substantial genetic variation for early life developmental traits in Crassostrea gigas, these culling practices are likely to cause highly different selective pressures in hatcheries from those in the natural environment. We studied the phenotypic and genetic...
Tipo: Text
Palavras-chave: Oysters; Larvae; Hatchery; Genetic diversity; Culling; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-1677.pdf

Single Nucleotide polymorphisms and their relationship to codon usage bias in the Pacific oyster Crassostrea gigas
Provedor de dados: 5
Autores: Sauvage, Christopher; Bierne, N; Lapegue, Sylvie; Boudry, Pierre.
DNA sequence polymorphism and codon usage bias were investigated in a set of 41 nuclear loci in the Pacific oyster Crassostrea gigas. Our results revealed a very high level of DNA polymorphism in oysters, in the order of magnitude of the highest levels reported in animals to date. A total of 290 single nucleotide polymorphisms (SNPs) were detected, 76 of which being localised in exons and 214 in non-coding regions. Average density of SNPs was estimated to be one SNP every 60 bp in coding regions and one every 40 bp in non-coding regions. Non-synonymous substitutions contributed substantially to the polymorphism observed in coding regions. The non-synonymous to silent diversity ratio was 0.16 on average, which is fairly higher to the ratio reported in other...
Tipo: Text
Palavras-chave: Crassostrea gigas; Codon bias; Genetic diversity; SNP.
Ano: 2007
URL: http://archimer.ifremer.fr/doc/2007/publication-3417.pdf

Genetic diversity of the European oyster (Ostrea edulis L.) in Nova Scotia: Comparison with other parts of Canada, Maine and Europe and implications for broodstock management
Provedor de dados: 5
Autores: Vercaemer, Bénédikte; Spence, Koren R.; Herbinger, Christophe; Lapegue, Sylvie; Kenchington, Ellen L.
The European oyster (Ostrea edulis) was introduced to the Nova Scotia aquaculture industry 30 years ago using stocks imported from naturalized populations in Maine whose ancestors originated in the Netherlands. This study used 5 microsatellites to assess the level of genetic diversity in several hatchery stocks and naturalized populations from Nova Scotia, New Brunswick, British Columbia and Maine. Some genetic erosion was shown to have occurred in the Maritimes populations, with the largest loss of alleles being experienced by the hatchery stocks. In spite of this loss, genetic diversity and heterozygosity in the Maritimes populations are still relatively high. Relationships within and between the populations and the existence of kin groups within the...
Tipo: Text
Palavras-chave: Genetic diversity; Maine; Microsatellites; Flat oyster; European oyster; Ostrea edulis.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/publication-2121.pdf

Variance of reproductive success in the European flat oyster Ostrea edulis assessed by microsatellite-based parentage analyses in a natural population and in hatchery
Provedor de dados: 5
Autores: Lallias, Delphine; Taris, Nicolas; Boudry, Pierre; Bonhomme, Francois; Lapegue, Sylvie.
he European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. Previous studies of allozymes, microsat-ellites and mitochondrial differentiation over the whole range concluded that a significant divergence existed between Mediterranean and Atlantic populations, together with an isolation-by-distance pattern. However, the average mitochondrial haplotypic diversity displayed a high among populations variance, reflecting smaller effective population size in some locations. Additionally, a ten-fold quantitative difference was observed in the same study in Fst between the mitochondrial and the nuclear genomes,...
Tipo: Text
Palavras-chave: Genotypes; Genetic diversity; Breeding success; Allozymes; Population genetics; Parentage analyses; Natural population; Ostrea edulis; Oyster.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/acte-3334.pdf

Recent population expansion and connectivity in the hydrothermal shrimp Rimicaris exoculata along the Mid-Atlantic Ridge
Provedor de dados: 5
Autores: Teixeira, Sara; Cambon-bonavita, Marie-anne; Serrao, Ester A.; Desbruyeres, Daniel; Arnaud-haond, Sophie.
Aim Deep-sea hydrothermal vents are unstable habitats that are both spatially and temporally fragmented. In vent species, a 'short-term insurance' hypothesis would lead us to expect mostly self-recruitment, limiting the loss of larvae in the deep ocean or water column and increasing genetic differentiation over the time elapsed since colonization. Alternatively, a 'long-term insurance' hypothesis would support the prediction of selection for large-scale dispersal, to ensure long-term persistence in these ephemeral habitats. The main goal of this study was to infer the spatial and temporal distribution of genetic diversity of the shrimp Rimicaris exoculata, which forms high-density local populations on hydrothermal vents along the Mid-Atlantic ridge....
Tipo: Text
Palavras-chave: Bottleneck; Deep-sea biogeography; Dispersal; Genetic diversity; Hydrothermal vents; Marine biogeography; Mid-Atlantic Ridge; Rimicaris exoculata; Spatio-temporal distribution.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00033/14394/12350.pdf

Gestion des ressources génétiques de l'huître perlière Pinctada margaritifera de Polynésie française : caractérisation génétique des populations et optimisation du recrutement pour l'exploitation perlière
Provedor de dados: 5
Autores: Arnaud, Sophie; Goyard, Emmanuel; Prou, Jean; Vonau, Vincent; Bonhomme, Francois; Boudry, Pierre.
Exploitation of the pearl oyster Pinetada margaritifera, which has developed in French Polynesia over the last twenty years, is based mainly on the collection of natural spat. Recruitment is highly variable in space and time and so spat is very often transferred by the farmers between atolls or even between archipelagos. In previous studies, anonymous nuclear markers demonstrated that This development has coincided with a genetic homogenisation of the wild stocks since the 1980s. This suggests a high level of reproductive success on farms, which is likely to be due to the high density of animaIs when compared to the density in the wild. We used the same markers to show that the level of genetic variability observed on farms is not significantly different...
Tipo: Text
Palavras-chave: French Polynesia; Genetic diversity; Spat; Pinctada margaritifera; Pearl oyster; Polynésie française; Diversité génétique; Naissain; Pinctada margaritifera; Huître perlière.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3822.PDF

Genetic and reproduction strategy in Crassostrea gigas mortality
Provedor de dados: 5
Autores: Moal, Jeanne; Bedier, Edouard; Fleury, Pierre-gildas; Langlade, Aime; Le Coguic, Yvette; Degremont, Lionel; Boudry, Pierre; Le Coz, Jean-rene; Pouvreau, Stephane; Enriquez-diaz, M.; Lambert, Christophe; Quere, Christele; Soudant, Philippe; Samain, Jean-francois.
Bi-parental families were produced in hatchery and tested in the field in 2001. Two sets of 5 families were constituted, selected on their high (R) and low (S) survival. These two sets were reared in Brittany from March to November 2002. Samplings were performed twice a month to obtain data on biometry, survival, reproductive cycle, biochemical composition, adenylate energy charge, hemolymph parameters (ions and defense system) and muscle strength. "R" and "S" oysters exhibited different reproductive effort and spawning strategy. "R" oysters allocated less energy in gonad than "S" ones and presented a complete spawning at the end of August contrary to the "S" which spawns partially. Mortality started in July when the seawater temperature reached 19 degree...
Tipo: Text
Palavras-chave: Summer; Reproduction; Oyster culture; Mortality; Genetic diversity; Commercial species; Breeding.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/acte-3345.pdf

Acquisition de données complémentaires aux dénombrements, avec les techniques d’analyses pigmentaires et de diversité génétique. Etat d’avancement et premiers résultats. Action Indice Composition. Livrable n° AIV. Rapport final, 16 juillet 2014.
Provedor de dados: 5
Autores: Delmas, Daniel; Siano, Raffaele.
Studies on phytoplankton community diversity cannot be limited microphytoplankton (cells&gt;20 ìm), it is indeed necessary to take into account also the nano (3-20ìm) picophytoplankton (&lt;3 ìm) components. In the frame of this work aimed at studying temporal phytoplankton community diversity two complementary methodologies of phytoplankton sample analysis, pigment and genetic (metarbarcoding) diversity, have been used as alternative to routine morphological analysis carried out at light microscopy. The REPHY station (047-P-016 Concarneau Large) off-shore the Concarneau Bay has been sampled during the annual highest phytoplankton production (March-July 2012) with a frequent sample interval strategy (twice per week). Results obtained by both pigment and...
Tipo: Text
Palavras-chave: Phytoplancton; Structure de taille; Picophytoplancton; Nanophytoplancton; Microphytoplancton; Diversité génétique; Metabarcoding; Taxonomie pigmentaire; Façade atlantique; Baie de Concarneau; Phytoplankton; Size classes; Picophytoplankton; Nanophytoplankton; Microphytoplankton; Genetic diversity; Metabarcoding; Pigment taxonomy; Atlantic coasts; Concarneau Bay.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00361/47260/47225.pdf

Impact of the reproduction dynamics on genetic variation in the European flat oyster Ostrea edulis
Provedor de dados: 5
Autores: Lapegue, Sylvie; Taris, Nicolas; Lallias, Delphine; Bonhomme, Francois; Boudry, Pierre.
The European flat oyster (Ostrea edulis L.) is a marine bivalve whose natural geographical distribution ranges along the European Atlantic coast from Norway to Morocco, in addition to the Mediterranean and Black Sea. The latest results obtained on the genetic differentiation between these populations have led us to pursue studies at a finer scale, in order to estimate the effective number of breeders and the temporal dynamics of reproduction and, more specially, recruitment. Several experiments were performed to document (1) the variance in allele frequencies during a natural settlement period; (2) the paternal contribution to fertilization by analyzing larvae sampled at the brooding stage within individual females; (3) the variance of individual...
Tipo: Text
Palavras-chave: Ostrea edulis; Oyster; Sexual reproduction; Population dynamics; Oyster culture; Inbreeding; Heterosis; Genetic isolation; Genetic diversity; Breeding success; Biological fertilization.
Ano: 2004
URL: http://archimer.ifremer.fr/doc/2004/acte-3436.pdf

Génotypage des géniteurs de Lates calcarifer de Tahiti : aide à la domestication raisonnée du Loup Tropical pour la filière tahitienne
Provedor de dados: 5
Autores: Vonau, Vincent; Rouxel, Catherine; Saulnier, Denis; Cochennec-laureau, Nathalie; Nedelec, Georges; Goyard, Emmanuel.
The tropical seabass Lates calcarifer was introduced in Tahiti in 1984 and then was domesticated without other introduction. Three successive generations have been obtained in captivity. Tissue samples of the 38 animaIs which represent the total Tahitian broodstock were preserved in alcohol to be genotyped with four microsatellite markers (Yue et al., 2001). Three of four described markers were successfully transferred to the laboratory of genetics of Tahiti (marker LcaM01, LcaM02, LcaM03), as the revelation technology available locally did not allow the use of LcaM04 in routine. The results show that the genetic diversity of the tahitian broodstock is not equivalent of the one of the species, which could be explained by the small size of the founder...
Tipo: Text
Palavras-chave: Lates calcarifer; Marqueurs microsatellites; Reproducteurs; Variabilité génétique; Lates calcarifer; Microsatellite markers; Breeding stock; Genetic diversity.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/00142/25300/23372.pdf

Size limits regulation for Tuna: should we also consider the protection of large fish?
Provedor de dados: 5
Autores: Fromentin, Jean-marc.
A recent body of literature has demonstrated that older females produce larvae and offsprings that have much higher survival and growth than do younger adults and that they furthermore play a key role in the adaptability, persistence and productivity of the exploited fish populations. These findings, that have been shown for several pelagic, groundfish and rockfish species, are, thus, worth to consider for tuna and tuna-like species. The aim of this modest and brief paper is solely to draw the attention from the SCRS to this issue which may have considerable impacts on management regulations.
Tipo: Text
Palavras-chave: Age structure; Genetic diversity; Recruitment success; Sustainability; Management.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/00192/30333/28808.pdf

Viability selection creates negative heterozygosity-fitness correlations in female Black Grouse Lyrurus tetrix
Provedor de dados: 5
Autores: Soulsbury, Carl D.; Lebigre, Christophe.
There is widespread interest in the relationship between individual genetic diversity and fitness-related traits (heterozygosity-fitness correlations; HFCs). Most studies have found weak continuous increases of fitness with increasing heterozygosity, while negative HFCs have rarely been reported. Negative HFCs are expected in cases of outbreeding depression, but outbreeding is rare in natural populations. Negative HFCs may also arise through viability selection acting on low heterozygosity individuals at an early stage producing a skew in the heterozygosity distribution. We tested this idea using survival and clutch parameters (egg mass, egg volume, chick mass, clutch size) in female Black Grouse Lyrurus tetrix and carried out simulations to determine how...
Tipo: Text
Palavras-chave: Inbreeding; Outbreeding; Neutral loci; Selective mortality; Genetic diversity.
Ano: 2018
URL: https://archimer.ifremer.fr/doc/00423/53448/54340.pdf

Etude des processus de dérive et de sélection liés aux pratiques d'élevage en écloserie d'huître creuse
Provedor de dados: 5
Autores: Boudry, Pierre.
Genetic consequences of production of Pacific oyster larval in hatchery: drift and selective pressures related to rearing practices. In order to study the genetic consequences of production of oyster larvae in hatcheries, two factors were examined: the effects of discarding the smallest larvae (i.e. culling) and temperature effects. A mixed-family approach was used in order to infer the genetic composition of the larval population. The results show that high polymorphic microsatellite-based family assignment is a powerful tool for the study of bivalve larvae genetics. Culling by selective sieving is an advantageous practice at a phenotypic scale, but also represents a substantial risk for diversity loss if parentage assignment is not introduced as a...
Tipo: Text
Palavras-chave: Hatchery; Genetic diversity; Selection domestication; Larvae; Crassostrea gigas; Ecloserie; Diversité génétique; Sélection domestication; Larve; Crassostrea gigas.
Ano: 2006
URL: http://archimer.ifremer.fr/doc/2006/rapport-1459.pdf

Sex, size and timing: Sampling design for reliable population genetics analyses using microsatellite data
Provedor de dados: 5
Autores: Dubois, Quentin; Lebigre, Christophe; Schtickzelle, Nicolas; Turlure, Camille.
Population genetics is used in a wide variety of fields such as ecology and biodiversity conservation. How estimated genetic characteristics of natural populations can be influenced by the sampling design has been a long-standing concern. Multiple simulation and empirical studies illustrated the influence of both sample size and polymorphism of markers. However, our review of studies on butterfly population genetics indicates no consensus on sample size for the estimation of genetic diversity or differentiation. Furthermore, other aspects of sampling design (sex ratio and timing of sampling) were not addressed and their potential impact on genetic parameter estimates rarely explored. Using a large empirical dataset (with spatial and temporal replicates)...
Tipo: Text
Palavras-chave: Butterfly; Conservation; Genetic diversity; Metapopulation; Number of samples; Period of sampling; Population structure; Sampling scheme optimization; Sex ratio.
Ano: 2018
URL: http://archimer.ifremer.fr/doc/00416/52783/53664.pdf

In vivo effects of metaldehyde on Pacific oyster, Crassostrea gigas: comparing hemocyte parameters in two oyster families.
Provedor de dados: 5
Autores: Moreau, Pierrick; Burgeot, Thierry; Renault, Tristan.
Pollutants via run-off into the ocean represent a potential threat to marine organisms, especially bivalves such as oysters living in coastal environments. These organisms filter large volumes of seawater and may accumulate contaminants within their tissues. Pesticide contamination in water could have a direct or indirect toxic action on tissues or cells and could induce alteration of immune system. Bivalve immunity is mainly supported by hemocytes and participates directly by phagocytosis to eliminate pathogens. Some studies have shown that pesticides can reduce immune defences and/or modify genomes in vertebrates and invertebrates. Metaldehyde is used to kill slugs, snails and other terrestrial gastropods. Although metaldehyde has been detected in...
Tipo: Text
Palavras-chave: Immunity; Hemocytes; Metaldehyde; Pacific oyster; Flow cytometry; In vivo; Genetic diversity.
Ano: 2015
URL: http://archimer.ifremer.fr/doc/00195/30602/29059.pdf

Primers for the amplification of the MHC II beta chain exon 2 in the Atlantic goliath grouper (Epinephelus itajara)
Provedor de dados: 5
Autores: Silva-oliveira, Glaucia C.; Silva, Anete B. C.; Blanchard, Fabian; Nunes, Zelia; Torres, Rodrigo A.; Sampaio, Iracilda; Vallinoto, Marcelo.
In the present study we designed a pair of primers to amplify the exon 2 of the MHC II beta chain of the Atlantic goliath grouper, which is responsible for the recognition of pathogenic molecules and the regulation of the immune system. Future analyses of this region may provide an important database to understand the evolutionary processes affecting the populations of the goliath grouper, and to predict the conservation perspectives in the species.
Tipo: Text
Palavras-chave: Epinephelus itajara; Genetic diversity; MhcEit-DAB.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00210/32161/33469.pdf

Apport des informations moléculaires et cellulaires pour la caractérisation de la résistance de l'huître plate européenne vis-à-vis de la bonamiose, et pour la détection de signatures de la sélection naturelle
Provedor de dados: 5
Autores: Harrang, Estelle.
The European flat oyster, an endemic species from European coasts, has been classified in the category of "endangered and/or declining species" since 2003. Indeed, the natural beds of this oyster, consumed since ancient times, have gradually been decimated by over-exploitation and by successive emergence of parasitic diseases. The parasite that causes the disease called bonamiosis has contributed to drastically reduce the French and European aquacultural production of flat oyster. Marine bivalve molluscs display two specificities that restrict possibilities to fight against diseases: they are grown in an open environment, and possess an innate immune system lacking in adaptive response. In this context, the selection of animaIs naturally resistant to...
Tipo: Text
Palavras-chave: Huître plate Européenne; Ostre edulis; Bonamiose; Bonamia ostreae; Marqueurs SNP; Expression génique; Activité hémocytaire; Cartographie de liaison; QTL; EQTL; Populations naturelles; Diversité génétique; Sélection naturelle; European flat oyster; Ostrea edulis; Bonamiosis; Bonamia ostreae; SNP markers; Gene expression; Haemocytic activity; Linkage map; QTL; EQTL; Natural populations; Genetic diversity; Genetic structure; Natural selection.
Ano: 2012
URL: http://archimer.ifremer.fr/doc/00127/23785/21702.pdf

Genetic diversity and inter-specific relations of western Mediterranean relic Abies taxa as compared to the Iberian A. alba
Provedor de dados: 5
Autores: Dering, Monika; Sekiewicz, Katarzyna; Boratynska, Krystyna; Litkowiec, Monika; Iszkulo, Gregorz; Romo, Angel; Boratynski, Adam.
Several Abies species are currently present in the Mediterranean region and most of them are endemic taxa and tertiary relicts. Using six nuclear microsatellites, we studied the genetic structure and interspecific relationships among West Mediterranean firs, A. pinsapo (Spain), A. maroccana and A. tazaotana (Morocco). Based on the hypothesis that A. pinsapo could historically exchange genes with A. alba growing in the Pyrenees via secondary contact, we investigated the level of genetic admixture between these species using a Bayesian approach. The studied populations showed moderate genetic diversity (mean H-E=0.598) and a high level of genetic differentiation (F-ST=0.225) that was especially pronounced between A. alba and the African firs. All populations...
Tipo: Text
Palavras-chave: A. pinsapo; A. alba; Genetic diversity; Phylogeography; Gibraltar Strait; West Mediterranean firs.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00291/40224/82999.pdf

Occurrence of Deferribacterales and Entomoplasmatales in the deep-sea Alvinocarid shrimp Rimicaris exoculata gut
Provedor de dados: 5
Autores: Zbinden, Magali; Cambon-bonavita, Marie-anne.
The phylogenetic diversity of the gut microbial population of the hydrothermal shrimp Rimicaris exoculata was determined. The presence of micro-organisms in the hindgut of the shrimp was determined, and their morphology illustrated for the first time by transmission electron microscopy. DNA was extracted from the fore-, mid- and hindgut of shrimps collected at the Rainbow site, at the Mid-Atlantic Ridge, and the sequences of the small-subunit rRNA (16S rDNA) were analyzed. Seven different bacterial phylotypes were identified from the 90 clones screened. The sequences were affiliated to three major groups: epsilon-Proteobacteria (48,8%), Entomoplasmatales (23.3%) and representatives of the new phylum Deferribacteres (26.6%). These results show that the...
Tipo: Text
Palavras-chave: Metal bioremediation; Indigenous gut microllora; Genetic diversity; Shrimp; Hydrothermal vent.
Ano: 2003
URL: http://archimer.ifremer.fr/doc/2003/publication-778.pdf

Nomenclature for the Nameless: A Proposal for an Integrative Molecular Taxonomy of Cryptic Diversity Exemplified by Planktonic Foraminifera
Provedor de dados: 5
Autores: Morard, Raphael; Escarguel, Gilles; Weiner, Agnes K. M.; Andre, Aurore; Douady, Christophe J.; Wade, Christopher M.; Darling, Kate F.; Ujiie, Yurika; Seears, Heidi A.; Quillevere, Frederic; De Garidel-thoron, Thibault; De Vargas, Colomban; Kucera, Michal.
Investigations of biodiversity, biogeography, and ecological processes rely on the identification of "species" as biologically significant, natural units of evolution. In this context, morphotaxonomy only provides an adequate level of resolution if reproductive isolation matches morphological divergence. In many groups of organisms, morphologically defined species often disguise considerable genetic diversity, which may be indicative of the existence of cryptic species. The diversity hidden by morphological species can be disentangled through genetic surveys, which also provide access to data on the ecological distribution of genetically circumscribed units. These units can be identified by unique DNA sequence motifs and allow studies of evolutionary and...
Tipo: Text
Palavras-chave: Cryptic species; Genetic diversity; Planktonic foraminifera; Molecular nomenclature; MOTUs.
Ano: 2016
URL: https://archimer.ifremer.fr/doc/00383/49408/49900.pdf

Rare sex or out of reach equilibrium? The dynamics of F-IS in partially clonal organisms
Provedor de dados: 5
Autores: Reichel, Katja; Masson, Jean-pierre; Malrieu, Florent; Arnaud-haond, Sophie; Stoeckel, Solenn.
Background: Partially clonal organisms are very common in nature, yet the influence of partial asexuality on the temporal dynamics of genetic diversity remains poorly understood. Mathematical models accounting for clonality predict deviations only for extremely rare sex and only towards mean inbreeding coefficient (F-IS) over bar &lt; 0. Yet in partially clonal species, both F-IS &lt; 0 and F-IS &gt; 0 are frequently observed also in populations where there is evidence for a significant amount of sexual reproduction. Here, we studied the joint effects of partial clonality, mutation and genetic drift with a state-and-time discrete Markov chain model to describe the dynamics of F-IS over time under increasing rates of clonality. Results: Results of the...
Tipo: Text
Palavras-chave: Partial asexuality; Parthenogenesis; Mating system; Inbreeding coefficient; Heterozygote excess; Genetic diversity.
Ano: 2016
URL: http://archimer.ifremer.fr/doc/00344/45474/45019.pdf

Genetic diversity, multiplicity of infection and population structure of Schistosoma mansoni isolates from human hosts in Ethiopia
Provedor de dados: 5
Autores: Aemero, Mulugeta; Boissier, Jerome; Climent, Deborah; Mone, Helene; Mouahid, Gabriel; Berhe, Nega; Erko, Berhanu.
Background: Human intestinal schistosomiasis caused by Schistosoma mansoni and urinary schistosomiasis caused by Schistosoma haematobium are endemic in Ethiopia. Although schistosomes look morphologically uniform, there is variation in infectivity, egg productivity and virulence due to variation in their genetic make. Knowing the genetic diversity and population structure of S. mansoni isolates will enable to understand and consider the possible variability in terms of infectivity, egg productivity and virulence. Methods: Between 2010 and 2011, genetic diversity and population structure of Schistosoma mansoni isolates from four endemic areas of Ethiopia was assessed using previously published 11 polymorphic microsatellite loci. Miracidia were hatched from...
Tipo: Text
Palavras-chave: Schistosoma mansoni; Microsatellite; Genetic diversity; Population structure; Ethiopia.
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00302/41369/71868.pdf

High connectivity across the fragmented chemosynthetic ecosystems of the deep Atlantic Equatorial Belt: efficient dispersal mechanisms or questionable endemism?
Provedor de dados: 5
Autores: Teixeira, Sara; Olu, Karine; Decker, Carole; Cunha, Regina L.; Fuchs, Sandra; Hourdez, Stephane; Serrao, Ester A.; Arnaud-haond, Sophie.
Chemosynthetic ecosystems are distributed worldwide in fragmented habitats harbouring seemingly highly specialized communities. Yet, shared taxa have been reported from highly distant chemosynthetic communities. These habitats are distributed in distinct biogeographical regions, one of these being the so-called Atlantic Equatorial Belt (AEB). Here, we combined genetic data (COI) from several taxa to assess the possible existence of cryptic or synonymous species and to detect the possible occurrence of contemporary gene flow among populations of chemosynthetic species located on both sides of the Atlantic. Several Evolutionary Significant Units (ESUs) of Alvinocarididae shrimp and Vesicomyidae bivalves were found to be shared across seeps of the AEB. Some...
Tipo: Text
Palavras-chave: Atlantic equatorial belt; Chemosynthetic habitats; Deep-sea connectivity; Endemic bivalves; Endemic shrimp; Genetic diversity; Microsatellite markers; Mitochondrial COI gene.
Ano: 2013
URL: http://archimer.ifremer.fr/doc/00157/26789/24950.pdf

Structure et diversité génétique des populations de raie bouclée en Méditerranée
Provedor de dados: 5
Autores: Lecuyer, Romain.
Chondrichtyes are threatened by several disturbances among which is the significant development of fisheries during the 20th century. The Mediterranean sea has long been fished and keeps being intensely so by several nations that seek cost-effectiveness or food security above all. Such a background does not ease data recording and sharing for research purposes. This study mainly aims at assessing the population structure and status of thornback ray so as to avoid its deterioration due to bottom-trawl fisheries pressure, hardly reversible even with a sudden sound management. To do so, an extensive panel of nuclear genetic markers has been developed to analyze underlying structure along with differenciation and genetic diversity of Mediterranean populations....
Tipo: Text
Palavras-chave: Méditerranée; Structure de populations; Différenciation; Diversité génétique; SNP; Raja clavata; Mediterranean sea; Population structure; Differenciation; Genetic diversity; SNP; Raja clavata.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00667/77899/80091.pdf

D-loop mitochondrial genetic analysis in Abeerden angus old type from Argentina
Provedor de dados: 46
Autores: Villegas Castagnasso,E.E; Rogberg-Muñoz,A; Prando  ,A.J; Baldo,A; Giovambattista,G.
The massive use of reproductive breeding technologies (mainly Artificial Insemination and Embryo Transfer) resulted in an important decrease in the diversity in many breeds, and some genetic lines have been lost. Within the Angus breed, the &amp;#8220;New Type&amp;#8221; gain importance since 1970, leaving the &amp;#8220;Old Type&amp;#8221; to a reduced number of herds in the whole world. The objective of this work was to determine the genetic diversity in an &amp;#8220;Old Type&amp;#8221; herd in Argentina. DNA samples were analyzed for sequence variation in the hypervariable region of the mitochondrial DNA (D-loop). Sequence comparison and phylogenetic analyses revealed that haplotypes fell into European haplogroup (T3) in general, and in particular had...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MtDNA; Bos Taurus; Angus; Matrilineages origin; Genetic diversity.
Ano: 2015
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332015000300003

Somaclonal variation in Cereus peruvianus Mill. (Cactaceae): its potential to generate new varieties and broaden the species's genetic basis
Provedor de dados: 46
Autores: Resende Gazoli,Adriana; Mangolin,Claudete Aparecida; Machado,Maria de Fátima.
In current study Random Amplified DNA-Polymorphism markers were used to detect genetic variations in C. peruvianus somaclones and to examine relationships among somaclones and plants grown from seeds in cultivated populations. The possible association of DNA fragments with morphological traits of somaclones was also investigated. Polymorphism in somaclones (62.24%) was higher than that detected in cultivated plants (42.57%). The casual grouping of somaclones presented atypical and typical shoot phenotypes showed that the random amplifi ed DNA-fragment polymorphisms detected in present study were not associated with shoot morphologies. Similarity in somaclones and cultivated plants ranged from 70.2 to 93.7% and 76.7 to 86.8%, respectively. Thus, in vitro...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactus; Genetic diversity; Mandacaru; RAPD markers; Somaclonal variation.
Ano: 2010
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332010000100004

Phylogeography, genetic diversity and structure of the poecilosclerid sponge Phorbas fictitius at oceanic islands
Provedor de dados: 16
Autores: Xavier, J.R.; Soest, R.W.M. van; Breeuwer, J.A.J.; Martins, A.M.F.; Menken, S.B.J.
In this study we assessed the sequence variation in the I3-M11 partition of the mtDNA cytochrome c oxidase subunit I gene (COI) in ten populations of the Atlanto-Mediterranean demosponge Phorbas fictitius (Porifera: Poecilosclerida) at two spatial scales: a regional scale comparing mainland (Iberian) and insular (Macaronesian) populations, and a local (Archipelagic) scale focusing on different island populations of the Azores archipelago. A multiple approach combining diversity measures, FST estimates, phylogenetic inference and nested clade phylogeographic analysis was used to assess the genetic structure and elucidate the evolutionary history of this species. Genetic differentiation, based of FST estimates, was found among most populations at both scales...
Tipo: Article / Letter to the editor
Palavras-chave: Azores; Genetic diversity; Iberia; Macaronesia; Mitochondrial DNA; Porifera; 42.72.
Ano: 2010
URL: http://www.repository.naturalis.nl/record/355610

Social organisation of the northern giant mouse lemur Mirza zaza in Sahamalaza, north western Madagascar, inferred from nest group composition and genetic relatedness
Provedor de dados: 16
Autores: Rode, E.J.; Nekaris, K.A-I.; Markolf, M.; Schliehe-Diecks, S.; Seiler, M.; Radespiel, U.; Schwitzer, C.
Shelters such as leaf nests, tree holes or vegetation tangles play a crucial role in the life of many nocturnal mammals. While information about characteristics and availability of these resources may help in conservation planning, nest use gives an indication about a species’ social organisation. The northern giant mouse lemur (Mirza zaza) is threatened by habitat loss within its restricted range. Our aim was to examine nest site preferences of M. zaza and to explore the species’ social organisation by examining sleeping site aggregation size and genetic relatedness within and between such aggregations. In the Ankarafa Forest inside Sahamalaza – Iles Radama National Park, northwestern Madagascar, we radio-tagged five male and three female M. zaza and...
Tipo: Article / Letter to the editor
Palavras-chave: Conservation; Fragmentation; Genetic diversity; Nest utilisation; Sleeping site; Small population genetics; 42.84.
Ano: 2013
URL: http://www.repository.naturalis.nl/record/447982

Caracterização molecular e divergência genética de Bemisia tabaci (Genn.) (Hemiptera: Aleyrodidae) em diferentes culturas e locais de cultivo
Provedor de dados: 82
Autores: Fontes,Fernanda Von H M; Colombo,Carlos A; Lourenção,André L.
Knowledge on the genetic variation of populations of Bemisia tabaci (Genn.) can improve the understanding of genetic diversity found in their biotypes and, consequently, offer guidelines for its management. In this study, the molecular characterization was performed and genetic diversity data were obtained for this insect from three regions of Brazil on different crops [cotton and soybean (Mato Grosso - MT); cabbage (Distrito Federal - DF); soybean and potato (São Paulo - SP)], using RAPD markers. RAPD analysis indicated 80.6% polymorphic loci and the average genetic similarity obtained by the Jaccard coefficient was 0.67. The whitefly populations collected on potato (SP) and soybean (MT) had higher genetic diversity values (0.75 and 0.72, respectively)....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Silverleaf whitefly; RAPD-PCR; Biotype; Genetic diversity; Squash silvering.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1519-566X2010000200012

Population genetics of the endangered catfish Pseudoplatystoma magdaleniatum (Siluriformes: Pimelodidae) based on species-specific microsatellite loci
Provedor de dados: 83
Autores: García-Castro,Kevin León; Rangel-Medrano,José David; Landínez-García,Ricardo Marcel; Márquez,Edna Judith.
ABSTRACT The Neotropical catfish genus Pseudoplatystoma comprises eight species of large size, widely distributed in South American basins. The endangered species P. magdaleniatum is endemic to Magdalena basin (Colombia), experiences high fishing pressure and its population genetics is relatively unknown. To study the genetic status and structure of P. magdaleniatum, 25 species-specific polymorphic microsatellite loci were developed using next-generation sequencing and then tested in samples collected in the Magdalena-Cauca basin. Based on 15 of these loci, P. magdaleniatum showed a high number of alleles per locus (9-10), high values of observed (0.762-0.798) and expected (0.770-0.791) heterozygosities, recent reduction of population size and gene flow....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bottleneck; Freshwater fish; Gene flow; Genetic diversity; Molecular marker.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100215

Development and characterization of fifteen polymorphic microsatellite loci in Bryconamericus aff. iheringii (Teleostei: Characidae) and cross-amplification in related Characidae species
Provedor de dados: 83
Autores: Apolinário-Silva,Caroline; Ferreira,Dhiego G.; Cavenagh,Analiza F.; Aprígio,Nícollas G. O.; Galindo,Bruno A.; Carlsson,Jens; Sofia,Silvia H.
ABSTRACT Data on 15 novel microsatellite loci from the Neotropical fish Bryconamericus aff. iheringii are presented here. Analyses of 32 individuals from four different streams revealed 192 different alleles, ranging from four to 32 alleles per locus (mean of 12.8 per locus). Observed and expected heterozygosities ranged from 0.094 to 0.813 and 0.205 to 0.952, respectively. These loci showed high polymorphic information content and will be a resource for genetic studies of B. aff. iheringii. Furthermore, several loci also amplified other small Neotropical Characidae (Piabarchus stramineus and Piabina argentea) and should be useful for these species.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Lambarizinho; Molecular markers; Neotropical fish; SSR.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252018000100218

Individual contributions to pooled-milt fertilizations of silver catfish Rhamdia quelen
Provedor de dados: 83
Autores: Ribolli,Josiane; Zaniboni-Filho,Evoy.
Supplementary stocking of fish in natural environments is a way to mitigate or compensate for the changes imposed on wild populations by river damming. Since little is known about the genetic composition of the supplementary stocks obtained by pooled-milt fertilization, the aim of this study was to determine the individual contributions of male jundiá Rhamdia quelen to offspring. Sperm from four males were mixed using equal volume of sperm from each of the males to fertilize eggs from only one female, kept in three blend with six males and three females. The proportions of larvae sired by the different males were quantified using five polymorphic DNA microsatellite loci. Analysis of these loci allowed paternal determination of 84% of the progeny, at a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jundiá; Milt pool; Paternity; Genetic diversity.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400011

Population genetics of three threatened catfish species in heterogeneous environments of the Cauca River, Colombia
Provedor de dados: 83
Autores: Restrepo-Escobar,Natalia; Yepes-Acevedo,Anny Johanna; Márquez,Edna Judith.
ABSTRACT Neotropical catfishes Ageneiosus pardalis, Pimelodus grosskopfii and Sorubim cuspicaudus are migratory fishes of commercial importance that exhibit decreasing populations due to overfishing and other anthropic interventions. This study used species-specific microsatellite loci to test the hypothesis that threatened fish populations show genetic vulnerability signs and are genetically structured in the middle and lower sections of the Cauca River. The studied species exhibit genetic diversity levels higher than the average values reported for Neotropical Siluriformes; however, they seem to have suffered recent bottlenecks and they present significant endogamy levels that are higher for the critically endangered catfish P. grosskopfii. Furthermore,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Genetic diversity; Genetic structure; Microsatellites; Siluriformes.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100204

Development of microsatellite loci and population genetics in the bumblebee catfish species Pseudopimelodus atricaudus and Pseudopimelodus magnus (Siluriformes: Pseudopimelodidae)
Provedor de dados: 83
Autores: Rangel-Medrano,José David; Márquez,Edna Judith.
ABSTRACT The Neotropical freshwater catfish Pseudopimelodus atricaudus and Pseudopimelodus magnus are two recently discovered species endemic to the Colombian Magdalena-Cauca River basin. In this study, a set of 13 microsatellite loci were developed by using next generation sequence technology to assess the genetic diversity and population structure in P. atricaudus and test for cross-species amplification in P. magnus. Both species exhibited high genetic diversity (P. atricaudus: Na: 9.000 - 9.769 alleles/locus, Ho: 0.760 - 0.804, HE: 0.804 - 0.840; P. magnus: Na: 12.8 - 5.4 alleles/locus, Ho: 0.638 - 0.683, HE: 0.747 - 0.755) compared to the mean levels of genetic diversity reported for Neotropical Siluriformes, and lack of genetic differentiation among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colombia; Freshwater fish; Gene flow; Genetic diversity; Magdalena-Cauca basin.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100205

Electric eels galore: microsatellite markers for population studies
Provedor de dados: 83
Autores: Souza-Shibatta,Lenice; Ferreira,Dhiego G.; Santos,Kátia F.; Galindo,Bruno A.; Shibatta,Oscar A.; Sofia,Silvia H.; Giacomin,Renata M.; Bastos,Douglas A.; Mendes-Júnior,Raimundo N. G.; Santana,Carlos David de.
Abstract Fourteen novel microsatellite loci are described and characterized in two species of electric eels, Electrophorus variiand E. voltaifrom floodplains and rivers of the Amazon rainforest. These loci are polymorphic, highly informative, and have the capacity to detect reliable levels of genetic diversity. Likewise, the high combined probability of paternity exclusion value and low combined probability of genetic identity value obtained demonstrate that the new set of loci displays suitability for paternity studies on electric eels. In addition, the cross-amplification of electric eel species implies that it may also be useful in the study of the closely related E. electricus, and to other Neotropical electric fishes (Gymnotiformes) species as tested...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazon Rainforest; Electrophorus; Genetic diversity; Gymnotiformes; SSR.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252020000400202

Development of microsatellite loci and population genetics of the catfish Pimelodus yuma (Siluriformes: Pimelodidae)
Provedor de dados: 83
Autores: Joya,Cristhian Danilo; Landínez-García,Ricardo Marcel; Márquez,Edna Judith.
ABSTRACT Pimelodus yuma (formerly Pimelodus blochii) is a freshwater fish, endemic to the Colombian Magdalena-Cauca and Caribbean basins that experiences habitat disturbances resulting from anthropogenic activities. Due to the lack of information about the population genetics of this species, this study developed 14 species-specific microsatellite loci to assess the genetic diversity and population structure of samples from the lower section of the Cauca River. The studied species showed genetic diversity levels higher than the average values reported for Neotropical Siluriformes and significant inbreeding levels as was described for some congeners. Furthermore, P. yuma comprises two coexisting genetic groups that exhibit gene flow along the lower section...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Freshwater fish; Genetic diversity; Gene flow; Molecular marker; Next generation sequencing.
Ano: 2021
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100209

Type-specific PCR assays for Babesia bovis msa-1 genotypes in Asia: Revisiting the genetic diversity in Sri Lanka, Mongolia, and Vietnam
Provedor de dados: 19
Autores: Liyanagunawardena, Nilukshi; Sivakumar, Thillaiampalam; Kothalawala, Hemal; Silva, Seekkuge Susil Priyantha; Battsetseg, Badgar; Dinh Thi Bich Lan; Inoue, Noboru; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia bovis is the most virulent Babesia organism, resulting in a high mortality rate in cattle. The genetic diversity of B. bovis merozoite surface antigens (MSAs), such as MSA-1, MSA-2b, and MSA-2c, might be linked to altered immune profiles in the host animals. The present study aimed to develop type-specific PCR assays for Asian msa-1 genotypes, thereby re-analyzing the genetic diversity of msa-1 in Sri Lanka, Mongolia, and Vietnam. Specific primers were designed for nine Asian msa-1 genotypes, which had been detected based on the phylogeny constructed using msa-1 gene sequences retrieved from the GenBank database. Specificity of the type-specific PCR assays was confirmed using plasmids containing the inserts of msa-1 gene fragments that represent...
Palavras-chave: Asia; Babesia bovis; Genetic diversity; Msa-1; Type-specific PCR.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4489

Genetic diversity and antigenicity variation of Babesia bovis merozoite surface antigen-1 (MSA-1) in Thailand
Provedor de dados: 19
Autores: Tattiyapong, Muncharee; Sivakumar, Thillaiampalam; Takemae, Hitoshi; Simking, Pacharathon; Jittapalapong, Sathaporn; Igarashi, Ikuo; Yokoyama, Naoaki.
Babesia bovis, an intraerythrocytic protozoan parasite, causes severe clinical disease in cattle worldwide. The genetic diversity of parasite antigens often results in different immune profiles in infected animals, hindering efforts to develop immune control methodologies against the B. bovis infection. In this study, we analyzed the genetic diversity of the merozoite surface antigen-1 (msa-1) gene using 162 B. bovis-positive blood DNA samples sourced from cattle populations reared in different geographical regions of Thailand. The identity scores shared among 93 msa-1 gene sequences isolated by PCR amplification were 43.5-100%, and the similarity values among the translated amino acid sequences were 42.8-100%. Of 23 total clades detected in our...
Palavras-chave: Antigenicity variation; Babesia bovis; Msa-1; Genetic diversity; Thailand.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4490

Etude faunistique et écologique des annelides polychètes de la côte Nord-Est de la Tunisie (Peninsule du Cap Bon, Mediterranée Ouest)
Provedor de dados: 20
Autores: Zaabi-Sendi, Sana.
The polychaetes communities structure and functioning of the Cap Bon peninsula (Northeast coast of Tunisia), were analyzed from 52 stations from Sidi Daoud to Menzel Horr. The faunistic study reveals the presence of 238 polychaetes species among which 86 records are newly reported for the Tunisian coasts. The total number of polychaetes listed in Tunisia rises to 375 species.  The polychaete community structure at the sites was examined by multivariate methods. five main polychaetes communities were identified in the study area, the sandy sediment community (SS), the muddy sediments community (SV), the coarse sediment community (SG), the fine-muddy sediment community (SFV) and the Heterogeneous sediments community (HS). These communities are distributed...
Tipo: Theses and Dissertations
Palavras-chave: Seasonal variations; Genetic diversity; Polychaeta; Annelida.
Ano: 2013
URL: http://hdl.handle.net/1834/5480

Genetic Differentiation in West African Lagoon Fishes
Provedor de dados: 20
Autores: Agnese, J.F.
Most of the fishes living in the lagoons do not spend all their time in these brackishwaters. Sometimes, they go back to the river to spawn as in the case of freshwater fishes like Chrysichthys nigrodigitatus. Others like marine fishes (e.g., Ethmalosa jimbriata) feed or reproduce in lagoons for a time. Even true lagoon fishes are generally able to live in freshwater and/or in marine water. This is the case for two brackishwater tilapias, Sarotherodon melanotheron and Tilapia guineensis. Both are present in lagoons from Senegal to Congo (formerly Zaire). S. melanotheron is present in marine water near Dakar, Senegal, and in some freshwater lakes in Cote d'Ivoire. 1: guineensis is also present in freshwater in Cote d'Ivoire.
Tipo: Proceedings Paper
Palavras-chave: Lagoon fisheries; Genetic diversity; Brackish water; Lagoon fisheries; Http://aims.fao.org/aos/agrovoc/c_4157.
Ano: 2000
URL: http://hdl.handle.net/1834/325

Genética poblacional del camarón rosado Farfantepenaeus notialis (Decápoda: Penaeidae): variación espacio temporal de la diversidad y la estructura genética
Provedor de dados: 20
Autores: Robainas Barcia, A.
En este trabajo se analiza la variación espacio temporal de los patrones de diversidad genética y estructura poblacional del camarón rosado Farfantepenaeus notialis, en la plataforma Sur de Cuba. Se estudiaron, 22 loci aloenzimáticos, de los cuales seis resultaron polimórficos (Akp3, AmyB, Est3, Gdh, GP7 y Per1); cinco loci microsatélites (PnS01, PnS03, PnS04, PnS18 y PnS20); y un fragmento de 2027pb del ADNmt, en muestras procedentes de ocho localidades del Golfo de Ana María, tres del Golfo de Batabanó y una del Golfo de Guacanayabo, durante los años 1995, 1999 y 2003.  El análisis de las poblaciones en el año 1995, empleando marcadores nucleares, reveló diferencias genéticas significativas entre las poblaciones del Golfo de Ana María, como fue...
Tipo: Theses and Dissertations
Palavras-chave: Genetic factors; Genetic diversity; Shrimp culture.
Ano: 2004
URL: http://hdl.handle.net/1834/5511

Etude de la structure génétique des populations de la tortue marine Caretta caretta dans les eaux tunisiennes.
Provedor de dados: 20
Autores: Chaieb, O.
La tortue marine, la caouanne (Caretta caretta), est une espèce en danger d’extinction. Elle est caractérisée par un cycle biologique complexe se basant sur une série de transitions écologiques et géographiques nécessitant des migrations sur de longues distances. En Tunisie, plusieurs études ont été réalisées sur les activités de nidification et les interactions avec les activités de pêche, mais, aucune n’a porté sur l’étude génétique de cette espèce. Dans ce travail, nous nous sommes intéressés à l’analyse génétique de la population nidifiante en Tunisie et des populations des tortues se développant dans les zones néritiques tunisiennes.  La population nidifiante a été appréhendée par l’analyse des nouveau-nés échantillonnés durant une période de sept ans...
Tipo: Theses and Dissertations
Palavras-chave: Allozymes; DNA; Genetic diversity; Neritic province.
Ano: 2013
URL: http://hdl.handle.net/1834/5923

Lack of mtDNA differentiation among hamlets (Hypoplectrus, Serranidae).
Provedor de dados: 20
Autores: García-Machado, E.; Chevalier, P.; Solignac, M.
The hamlets are a group of vividly colored fish species of the Serranidae family differentiated only by the color pattern of the body. Although there are divergent views about hamlet taxonomy, experimental and field observations have shown a strong assortative mating, justifying a species status for the different color morphs. Here we analyze the level of evolutionary divergence among six species in respect of mitochondrial DNA, with a view to contrasting the pattern observed with color partitions and previous results obtained with isozymes. The estimated molecular distance among species was low and of the same magnitude as nucleotide diversity within species. Consequently, the net distance and hence the time of divergence between taxa was virtually zero...
Tipo: Journal Contribution
Palavras-chave: MtDNA; Marine fish; Genetic diversity.
Ano: 2003
URL: http://hdl.handle.net/1834/4569

Diversidad haplotípica en el manatí Trichechus manatus en Cuba:  Resultados preliminares
Provedor de dados: 20
Autores: Hernández Martínez, D.; Álvarez-Alemán, A.; Bonde, R. K.; Powell, J.A.; García-Machado, E.
El objetivo del presente trabajo fue obtener información preliminar acerca de la composición de haplotipos del ADN  mitocóndrico (ADNmt) del manatí (Trichechus manatus) que habita en el archipiélago cubano. Se analizó un total de 13  individuos, 12 de Cuba y uno procedente de la Florida, EU.  Las secuencias de un fragmento de 410 pb de la región de control  del ADNmt (D-loop) permitieron identificar dos haplotipos. El haplotipo A1, único encontrado en la Florida (incluida la  muestra aquí analizada), pero también presente en México, Puerto Rico y República Dominicana. Este haplotipo fue  mayoritario (11 de los 12 individuos muestreados en Cuba). El segundo haplotipo fue el A3, referido anteriormente como  endémico de Belice, se encontró  en un individuo...
Tipo: Journal Contribution
Palavras-chave: Genetic diversity; MtDNA.
Ano: 2013
URL: http://hdl.handle.net/1834/5252

The role of the Yala swamp lakes in conservation of Lake Victoria region haplochromine cichlids: evidence from molecular genetic and trophic ecology studies
Provedor de dados: 20
Autores: Abila, R.; Salzburger, W.; Ndonga, M.F.; Owiti, D.O.; Barluenga, M.
Lake Kanyaboli (Kenya), a satellite lake of Lake Victoria, has been suggested as a potential refugium for haplochromine cichlids that have gone extinct in Lake Victoria. We employed mitochondrial DNA and microsatellite DNA molecular markers as well as feeding ecology studies to re- evaluate the evolutionary and ecological significance of Lake Kanyaboli haplochromines. The mitochondrial DNA and microsatellite markers revealed high genetic diversity in the endangered Xystichromis phytophagus and also the presence of mtDNA haplotypes that may have either gone extinct in Lake Victoria or have arisen in situ. Lake Kanyaboli thus acts as a ‘genetic reservoir’ for the Lake Victoria species flock. Gut content analysis revealed six trophic groups among the six...
Tipo: Proceedings Paper
Palavras-chave: Tropical fish; Genetics; Swamps; Lakes; Resource conservation; Environment management; Rare resources; Resource management; Trophic relationships; Genetic diversity; Swamps; Inland waters; Water reservoirs; Freshwater lakes; Species extinction; Ecological efficiency; Genetics; Swamps; Lakes; Swamps; Inland waters; Water reservoirs; Species extinction; Resource conservation; Resource management; Http://aims.fao.org/aos/agrovoc/c_3222; Http://aims.fao.org/aos/agrovoc/c_7544; Http://aims.fao.org/aos/agrovoc/c_4160; Http://aims.fao.org/aos/agrovoc/c_7544; Http://aims.fao.org/aos/agrovoc/c_3876; Http://aims.fao.org/aos/agrovoc/c_8324; Http://aims.fao.org/aos/agrovoc/c_17744; Http://aims.fao.org/aos/agrovoc/c_6523; Http://aims.fao.org/aos/agrovoc/c_6524.
Ano: 2006
URL: http://hdl.handle.net/1834/2130

Expected gain in selection of Pinus elliottii var. elliottii for timber production at an early age
Provedor de dados: 190
Autores: Moreira, Juliana Prado; Shimizu, Jarbas Yukio; Sousa, Valderês Aparecida; Moraes, Mario Luis Teixeira de; Moura, Nara Fernandes; Aguiar, Ananda Virgínia de.
P. elliotii var. elliottii is the second most planted fast growing tree species in Brazilian subtropics for wood and resin production. The objective of this work was to predict genetic values of fast growing individual trees. A trial was established in a randomized complete blocks design involving 76 treatments, 75 open pollinated progenies from a clonal seed orchard and a commercial progeny as control, in a 3 m x 3 m spacing. Total heights of all individuals were measured at 1, 2, and 3 years old and also stem diameter at 1.3 m height- dbh at 3 years old. Deviance analysis was performed by using Selegen-REML/BLUP software and genetic parameters were estimated. Significant variation was detected among progenies. Estimates of narrow-sense individual...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: REML/BLUP; Genetic improvement; Genetic diversity; Repeatability; Heritability Melhoramento florestal REML/BLUP; Correlações; Diversidade genética; Ganho de seleção; Melhoramento genético.
Ano: 2014
URL: http://pfb.cnpf.embrapa.br/pfb/index.php/pfb/article/view/488

In situ and genetic characterization of Gossypium barbadense populations from the states of Pará and Amapá, Brazil
Provedor de dados: 86
Autores: Almeida,Vanessa Cavalcante de; Hoffmann,Lúcia Vieira; Yokomizo,Gilberto Ken It; Costa,Joaquim Nunes da; Giband,Marc; Barroso,Paulo Augusto Vianna.
The objective of this work was to characterize the populations of Gossypium barbadense in the states of Amapá and Pará, Brazil. In situ characterization was conducted through interviews with the owners of the plants and environmental observations. Leaf or petal tissue as well as seed samples were collected for genetic characterization by single sequence repeats markers and for storage in germplasm banks, respectively. The plants were maintained in dooryards and used mainly for medical purposes. The genetic analysis showed no heterozygous plants at the loci tested (f = 1), indicating that reproduction occurs mainly through selfing. The total genetic diversity was high (He = 0.39); and a high level of differentiation was observed between cotton plants from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cotton; Genetic diversity; Germplasm; SSR markers.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2009000700011

Evolutionary dynamics of Cucurbita argyrosperma from the Mesoamerican domestication center using SSR molecular markers
Provedor de dados: 86
Autores: Priori,Daniela; Villarreal,Daniel Zizumbo; Ek,Víctor Manuel de Jesús Canché; Limones-Briones,Verónica; Barbieri,Rosa Lía.
Abstract: The objective of this work was to evaluate the evolutionary dynamics of the wild-weedy-domestic gene pool of Cucurbita argyrosperma squash by estimating the levels of genetic diversity and gene flow in the putative area of its domestication. Nine populations were collected, and DNA was extracted from young leaves harvested separately from approximately 20 individuals in each population. The DNA fragments were amplified with eight pairs of SSR primers and separated by electrophoresis in 5% denaturing polyacrylamide gels. Genetic diversity and the amount of gene flow were estimated in the populations, and Bayesian grouping was used to determine the levels of gene infiltration and probability of ancestry. The ethnobotanical exploration indicated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cucurbitaceae; Genetic diversity; Microsatellite markers; Plant genetic resources; Squash.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000300287

Quantification of the diversity among common bean accessions using Ward-MLM strategy
Provedor de dados: 86
Autores: Cabral,Pablo Diego Silva; Soares,Taís Cristina Bastos; Gonçalves,Leandro Simões Azeredo; Amaral Júnior,Antonio Teixeira do; Lima,Andreia Barcelos Passos; Rodrigues,Rosana; Matta,Frederico de Pina.
The present work aimed at evaluating the divergence among common bean accessions by their agronomic, morphological and molecular traits, based on the Ward-MLM procedure. A collection of 57 accessions from the gene bank of Universidade Federal do Espírito Santo was used in this study, from which: 31 were landraces belonging to the community Fortaleza, in the municipality of Muqui, ES, Brazil; 20 accessions were provided by Embrapa Trigo; and 6 were commercial cultivars. Five agronomic traits (plant cycle, number of seeds per pod, number of pods per plant, weight of 100 seeds, and grain yield), five morphological traits (growth habit, plant size, seed shape, seed color, and commercial group) and 16 microsatellite primers were evaluated. High genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Phaseolus vulgaris; Genetic diversity; Germplasm characterization; Joint analysis; Molecular markers; Morphological markers.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2010001000011

Genetic variability in conilon coffee related to grain attributes in an irrigated crop in the Cerrado
Provedor de dados: 86
Autores: Brige,Felipe Augusto Alves; Celestino,Sonia Maria Costa; Amabile,Renato Fernando; Fagioli,Marcelo; Delvico,Francisco Marcos dos Santos; Montalvão,Ana Paula Leite; Sala,Pedro Ivo Aquino Leite.
Abstract: The objective of this work was to quantify the genetic variability of 213 conilon coffee accessions of the Robusta Tropical cultivar, based on chemical characteristics related to the quality of green coffee beans, as well as to identify promising accessions for the breeding program of irrigated conilon coffee in the Brazilian Cerrado. The chemical characteristics evaluated were: protein and caffeine contents, total soluble solids and total lipids, ether extract, pH, and total titratable acidity. The data were subjected to the principal component analysis and cluster analysis based on the similarities observed within the first two principal components using the minimum variance method (Wards) and, as a measure of similarity, the Euclidean...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea canephora; Chemical composition; Genetic diversity; Multivariate analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2019000102804

Genetic structure and gene flow of Eugenia dysenterica natural populations
Provedor de dados: 86
Autores: Zucchi,Maria Imaculada; Pinheiro,José Baldin; Chaves,Lázaro José; Coelho,Alexandre Siqueira Guedes; Couto,Mansuêmia Alves; Morais,Lizz Kezzy de; Vencovsky,Roland.
This study was carried out to assess the genetic variability of ten "cagaita" tree (Eugenia dysenterica) populations in Southeastern Goiás. Fifty-four randomly amplified polymorphic DNA (RAPD) loci were used to characterize the population genetic variability, using the analysis of molecular variance (AMOVA). A phiST value of 0.2703 was obtained, showing that 27.03% and 72.97% of the genetic variability is present among and within populations, respectively. The Pearson correlation coefficient (r) among the genetic distances matrix (1 - Jaccard similarity index) and the geographic distances were estimated, and a strong positive correlation was detected. Results suggest that these populations are differentiating through a stochastic process, with restricted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Myrtaceae; Cerrado; Tropical species; Genetic diversity.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2005001000005

Molecular characterization of soybean cultivars by microsatellite markers with universal tail sequence
Provedor de dados: 86
Autores: Ribeiro,Carlos Alexandre Gomes; Tanure,Janaína Paula Marques; Maciel,Talles Eduardo Ferreira; Barros,Everaldo Gonçalves de.
The objective of this work was to standardize a semiautomated method for genotyping soybean, based on universal tail sequence primers (UTSP), and to compare it with the conventional genotyping method that uses electrophoresis in polyacrylamide gels. Thirty soybean cultivars were genotypically characterized by both methods, using 13 microsatellite loci. For the UTSP method, the number of alleles (NA) was 50 (2-7 per marker) and the polymorphic information content (PIC) ranged from 0.40 to 0.74. For the conventional method, the NA was 38 (2-5 per marker) and the PIC varied from 0.39 to 0.67. The genetic dissimilarity matrices obtained by the two methods were highly correlated with each other (0.8026), and the formed groups were coherent with the phenotypic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; Cultivar protection; Genetic diversity; Genotyping method; Random identity probability.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000300005

Functional diversity of bacterial genes associated with aromatic hydrocarbon degradation in anthropogenic dark earth of Amazonia
Provedor de dados: 86
Autores: Germano,Mariana Gomes; Cannavan,Fabiana de Souza; Mendes,Lucas William; Lima,Amanda Barbosa; Teixeira,Wenceslau Geraldes; Pellizari,Vivian Helena; Tsai,Siu Mui.
The objective of this work was to evaluate the catabolic gene diversity for the bacterial degradation of aromatic hydrocarbons in anthropogenic dark earth of Amazonia (ADE) and their biochar (BC). Functional diversity analyses in ADE soils can provide information on how adaptive microorganisms may influence the fertility of soils and what is their involvement in biogeochemical cycles. For this, clone libraries containing the gene encoding for the alpha subunit of aromatic ring-hydroxylating dioxygenases (α-ARHD bacterial gene) were constructed, totaling 800 clones. These libraries were prepared from samples of an ADE soil under two different land uses, located at the Caldeirão Experimental Station - secondary forest (SF) and agriculture (AG) -, and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Catabolic genes; Genetic diversity; Land use systems; Microbial ecology; Tropical soils.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2012000500004

Morphological and molecular diversity among cassava genotypes
Provedor de dados: 86
Autores: Mezette,Thiago Fonseca; Blumer,Caroline Groppo; Veasey,Elizabeth Ann.
The objective of this work was to characterize morphologically and molecularly the genetic diversity of cassava accessions, collected from different regions in Brazil. A descriptive analysis was made for 12 morphological traits in 419 accessions. Data was transformed into binary data for cluster analysis and analysis of molecular variance. A higher proportion of white or cream (71%) root cortex color was found, while flesh colors were predominantly white (49%) and cream (42%). Four accession groups were classified by the cluster analysis, but they were not grouped according to their origin, which indicates that diversity is not structured in space. The variation was greater within regions (95.6%). Sixty genotypes were also evaluated using 14 polymorphic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites; Morphological traits.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2013000500007

Genetic variability of hull-less barley accessions based on molecular and quantitative data
Provedor de dados: 86
Autores: Sayd,Ricardo Meneses; Amabile,Renato Fernando; Faleiro,Fabio Gelape; Bellon,Graciele.
The objective of this work was to characterize and quantify the genetic, molecular, and agronomic variability of hull-less barley genotypes, for the selection of parents and identification of genotypes adapted to the irrigated production system in the Brazilian Cerrado. Eighteen hull-less barley accessions were evaluated, and three covered barley accessions served as reference. The characterization was based on 157 RAPD molecular markers and ten agronomic traits. Genetic distance matrices were obtained based on molecular markers and quantitative traits. Graphic grouping and dispersion analyses were performed. Genetic, molecular, and agronomic variability was high among genotypes. Ethiopian accessions were genetically more similar, and the Brazilian ones...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Hordeum vulgare; Genetic diversity; Molecular markers; Quantitative traits.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2015000200160

Bacterial diversity of soil under eucalyptus assessed by 16S rDNA sequencing analysis
Provedor de dados: 86
Autores: Silveira,Érico Leandro da; Pereira,Rodrigo Matheus; Scaquitto,Denilson César; Pedrinho,Eliamar Aparecida Nascimbém; Val-Moraes,Silvana Pómpeia; Wickert,Ester; Carareto-Alves,Lúcia Maria; Lemos,Eliana Gertrudes de Macedo.
Studies on the impact of Eucalyptus spp. on Brazilian soils have focused on soil chemical properties and isolating interesting microbial organisms. Few studies have focused on microbial diversity and ecology in Brazil due to limited coverage of traditional cultivation and isolation methods. Molecular microbial ecology methods based on PCR amplified 16S rDNA have enriched the knowledge of soils microbial biodiversity. The objective of this work was to compare and estimate the bacterial diversity of sympatric communities within soils from two areas, a native forest (NFA) and an eucalyptus arboretum (EAA). PCR primers, whose target soil metagenomic 16S rDNA were used to amplify soil DNA, were cloned using pGEM-T and sequenced to determine bacterial diversity....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Metagenomic; Microbial communities.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2006001000008

AFLP polymorphism in restored provenances of Ceiba aesculifolia within an urban heat island
Provedor de dados: 85
Autores: Olvera-Mendoza,EI; Lara-Cabrera,SI; Sáenz-Romero,C; Lindig-Cisneros,R.
To quantify the effect on genetic diversity of restoring tree populations in an area under the influence of an urban heat island, we evaluated the genetic diversity of 72 Ceiba aesculifolia individuals, from a restoration experiment established from 2170 to 2260 m a.s.l. Reintroduced individuals were compared with the provenance from which the seeds were obtained, and two external provenances, for a total of 123 individuals. Samples were analyzed with three AFLP primer combinations. Polymorphisms of 38.4 to 62.5% were obtained. Genetic diversity estimated with the Simpson index ranged from 0.14 to 0.2. The provenances in the restoration site had higher diversity than the provenance that originated the seeds. Groups formed with provenances with similar...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; AFLP; Restoration ecology; Ceiba aesculifolia; Protected Natural Area.
Ano: 2016
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572016000200001

Variability and Genetic Structure in Populations of Arrabidaea bilabiata (Sprague) Sandwith in the Amazonas State
Provedor de dados: 87
Autores: Souza,L.S.A.; Silva,J.F.; Lopes,M.T.G.; Bentes,J.L.S.; Valente,M.S.F.; Costa Neto,P.Q.
Weeds in pastures can intoxicate animals, and Arrabidaea bilabiata is the most important species for herbivores in floodplain areas in the Amazon Basin. Genetic diversity studies in natural populations may contribute to the better understanding of the range of toxicity and the genetic variability organization in this species. The objective of this study was to assess the variability and genetic structure in six populations of A. bilabiata sampled in floodplain areas in three municipalities of the Amazonas State, from the AFLP markers analysis. AFLP markers were efficient to characterize the genetic variability of the 65 individuals analyzed. From four combinations of oligonucleotides, a total of 309 AFLP fragments was obtained, where 304 (98.38%) were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Arrabidaea bilabiata; Pasture weeds; Poisonous plants; AFLP; Genetic diversity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582015000200213

Exploring Parthenium weed biotypes by chloroplast DNA barcode analysis
Provedor de dados: 87
Autores: Anjum,Tehmina; Jabeen,Rasheda; Adkins,Steve; Akram,Waheed.
Abstract  Background:  Parthenium weed (Parthenium hysterophorus L.) is an invasive weed that has invaded vast regions of Pakistan in a relatively very short period of a decade or two, threatening the crop fields of the agrarian fed country. Parthenium hysterophorus L. is native of central South America and Gulf of Mexico, has now turned out to be a weed of global significance due to its alarming invasions and profuse spread in approximately all parts of the world. Its invasion is probably due to the contamination of its seeds in the imported grains from other countries of the world.  Objective:  During comprehensive sampling from Pakistan and Australia, it was observed that parthenium weed accessions exhibited several distinct morphological features...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Parthenium hysterophorus; Variations; Genetic diversity; MEGA; Haplotypes.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582020000100302

Genetic diversity among proso millet (Panicum miliaceum) biotypes assessed by AFLP technique
Provedor de dados: 87
Autores: Karam,D.; Westra,P.; Nissen,S.J.; Ward,S.M.; Figueiredo,J.E.F.
The Amplified Fragment Length Polymorphism (AFLP) technique was used to access genetic diversity between three domestic and nine wild proso millet biotypes from the United States and Canada. Eight primer combinations detected 39 polymorphic DNA fragments, with the genetic distance estimates among biotypes ranging from 0.02 to 0.04. Colorado-Weld County black seeded and Wyoming-Platte County were the most distinct biotypes according to the dissimilarity level. A UPGMA cluster analysis revealed two distinct groups of proso millet without any geographic association. Six weed biotypes exhibiting some characters of cultivated plants were grouped together with domesticated biotypes of proso millet while the three typical wild phenotypes were clearly clustered...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Proso millet; AFLP; Taxonomic relationship; Genetic diversity.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-83582004000200001

Crop Genetic Resources:  An Economic Appraisal
Provedor de dados: 31
Autores: Day-Rubenstein,   Kelly A.; Heisey,   Paul W.; Shoemaker,   Robbin A.; Sullivan,   John; Frisvold,   George B.
Crop genetic resources are the basis of agricultural production, and significant economic benefits have resulted from their conservation and use. However, crop genetic resources are largely public goods, so private incentives for genetic resource conservation may fall short of achieving public objectives. Within the U.S. germplasm system, certain crop collections lack sufficient diversity to reduce vulnerability to pests and diseases. Many such genetic resources lie outside the United States. This report examines the role of genetic resources, genetic diversity, and efforts to value genetic resources. The report also evaluates economic and institutional factors influencing the flow of genetic resources, including international agreements, and their...
Tipo: Report
Palavras-chave: Genetic resources; Genetic diversity; Germplasm; R&amp;D; International transfer of genetic resources; In situ conservation; Ex situ conservation; Gene banks; Intellectual property.; Crop Production/Industries; Research and Development/Tech Change/Emerging Technologies.
Ano: 2005
URL: http://purl.umn.edu/59388

Wheat Diversity and Productivity in Indian Punjab After the Green Revolution
Provedor de dados: 31
Autores: Smale,   Melinda; Singh,   Joginder; Di Falco,   Salvatore; Zambrano,   Patricia.
The Punjab of India is an historical source of key wheat genetic resources in national and global plant breeding, and a focus of concerns about the abandonment of local varieties during the Green Revolution. Much of the wheat area in Punjab was already planted with earlier products of modern plant breeding programs when the Green Revolution began. These cultivars were more genetically similar and less productive than the semi-dwarf wheat varieties that succeeded them. We define, summarize and test indices of variety change and genetic diversity for the modern wheat varieties released and grown in Indian Punjab during the post-Green Revolution period. The first is the area-weighted average of varieties grown, which measures the rate of variety change,...
Tipo: Conference Paper or Presentation
Palavras-chave: Wheat; Genetic diversity; Indian Punjab; Productivity; Crop Production/Industries; Q12; Q57.
Ano: 2006
URL: http://purl.umn.edu/25794

Should Europe Further Strengthen Intellectual Property for Plant  Breeders? An Analysis of Seed Industry Proposals
Provedor de dados: 31
Autores: Eaton,   Derek J.F.; van Tongeren,   Frank W.
This paper illustrates the potential negative effects of increasing the scope of plant breeders' rights (PBR) protection, as has been proposed for Europe by leading plant breeding firms. Such a policy could increase the costs for varietal development for breeding companies, particularly if their access to varieties of the market leader is constrained. This is represented as an  asymmetrical increase in breeders' cost functions in a simple model of endogenous quality choice under price competition. Increased scope of IPR protection leads to increased profits for the leading breeding company but decreases in varietal quality and both farm and overall profits.
Tipo: Conference Paper or Presentation
Palavras-chave: Intellectual property rights; Product differentiation; Plant breeding; Genetic diversity; Research and Development/Tech Change/Emerging Technologies; L13; O34; Q16.
Ano: 2005
URL: http://purl.umn.edu/24725

Farmer management of production risk on degraded lands: the role of wheat genetic diversity in Tigray Region, Ethiopia
Provedor de dados: 31
Autores: Di Falco,   Salvatore; Chavas,   Jean-Paul; Smale,   Melinda.
This paper investigates the effects of wheat genetic diversity and land degradation on risk and agricultural productivity in less favored production environments of a developing agricultural economy. Drawing production data from household survey conducted in the highlands of Ethiopia, we estimate a stochastic production function to evaluate the effects of variety richness, land degradation, and their interaction on the mean and the variance of wheat yield. Ethiopia is a centre of diversity for durum wheat and farmers manage complex variety mixtures on multiple plots. Econometric evidence shows that variety richness increases farm productivity. Variety richness also reduces yield variability but only for high levels of genetic diversity. Simulations with...
Tipo: Report
Palavras-chave: Land degradation; Wheat production; Productivity; Risk; Genetic diversity; Household surveys; Biodiversity; Stochastic analysis; Crop Production/Industries; Risk and Uncertainty.
Ano: 2006
URL: http://purl.umn.edu/55417

Assessment of silver-stained AFLP markers for studying DNA polymorphism in proso millet (Panicum miliaceum L.)
Provedor de dados: 88
Autores: Karam,Décio; Westra,Philip; Niessen,Scott J.; Ward,Sarah M.; Figueiredo,José Edson Fontes.
Proso millet (Panicum miliaceum L.) is a serious weed in North America. A high number of wild proso millet biotypes are known but the genetic basis of its phenotypic variation is poorly understood. In the present study, a non-radioactive silver staining method for PCR-Amplified Fragment Length Polymorphism (AFLP) was evaluated for studying genetic polymorphism in American proso millet biotypes. Twelve biotypes and eight primer combinations with two/three and three/three selective nucleotides were used. Pair of primers with two/three selective nucleotides produced the highest number of amplified DNA fragments, while pair of primers with three/three selective nucleotides were more effective for revealing more polymorphic DNA fragments. The two better primer...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP; Genetic diversity; Molecular markers; Proso-millet.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042006000400011

Allozyme variation in a natural population of Stryphnodendron adstringens in the Rio Preto State Park, southeastern Brazil
Provedor de dados: 88
Autores: Glasenapp,Jacqueline Siqueira; Barbosa,Priscila Barros; Casali,Vicente Wagner Dias; Martins,Ernane Ronie; Cruz,Cosme Damião.
Leaves and fruits from 63 Stryphnodendron adstringens trees were sampled in the Rio Preto State Park to analyze allozyme segregation, tissue specific expression of allozyme loci, and their genetic parameters. The enzyme systems ADH, EST, ACP, PGM, PGI, GDH, G6PDH, GOT, IDH, LAP, MDH, PER and SKDH were assessed by means of starch-gel electrophoresis. The polymorphic systems PGI, IDH, MDH and GOT demonstrated a dimeric quaternary structure, while EST and PER were monomeric. The total expected genetic diversity (H E) for leaves and seeds were 0.325 and 0.244 respectively. The effective number of alleles per locus (A E) was 1.58 in leaves and 1.42 in seeds. The values of H E and A E observed in S. adstringens were comparatively higher than the average values...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozyme segregation; Genetic diversity; Heterozygosity; Medicinal plants.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042012000200003

Development and Application of Est-Ssr Markers in Quails
Provedor de dados: 89
Autores: Bai,JY; Pang,YZ; Qi,YX; Zhang,XH; Yun,XY.
ABSTRACT Aiming at accelerating the application of molecular markers in the genetic improvement of quails, six EST-SSR markers were successfully developed using a bioinformatics method. Polymorphisms of three quail populations (Chinese yellow quail, China black quail and Korean quail) were detected. The results showed that there were 2-6 alleles in six EST-SSR markers. Mean polymorphism information contents of Chinese yellow quails, Chinese black quails and Korean quails were determined as 0.5451, 0.4962 and 0.4937, respectively. Average heterozygosity valuesof 0.6134, 0.5759 and 0.5613 were calculated. Among the six EST-SSR markers, three were highly polymorphic, and the other three were moderately polymorphic. The newly-developed six EST-SSR markers may...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese yellow quail EST-SSR Bioinformatics; Genetic diversity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000500027

Microsatellite Analysis of Genetic Diversity in Quail Populations from China
Provedor de dados: 89
Autores: Bai,JY; Pang,YZ; Zhang,XH; Yun,YX; Qi,YX.
ABSTRACT Polymorphism of three quail communities was analyzed by using 12 microsatellite markers in this paper, aiming to provide scientific references for the evaluation, protection and utilization of quail genetic resources in China. Results demonstrated that the number of observed alleles by 12 microsatellite markers ranges between 4~7. The average polymorphism information contents (PIC) of the Chinese yellow quail, the Chinese black quail and the Korean quail, as detected by 12 microsatellite markers, are 0.6853, 0.6401 and 0.6565,respectively, and average heterozygosity values are 0.7333, 0.6957 and 0.7111, respectively. This indicates that the Chinese yellow quail has the richest genetic polymorphism. According to cluster analysis, the Chinese black...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chinese black quail; Microsatellite marker; Polymorphism; Genetic diversity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000300519

Characterization of Hilly Chickens in Consideration of Climate Change Factors: Light and Heat
Provedor de dados: 89
Autores: Khan,MKI; Ali,M; Akter,MA; Zaman,S; Miah,G.
ABSTRACT Hilly chickens were characterized from January 2015 to September 2016 considering climate factors (light and heat). The experimental birds were divided into three groups (heat stress; light and control). The heat was generated in the chicken’s shelter by a black shaded light (Lantern) for two to three hours more after sunset. A lighting device (Lantern) was kept in the chicken’s shelter for the same period for increasing daylight duration. The individual chicken’s egg production, egg weight and mature live weight were studied from onset of egg production. It was observed that reddish brown hilly type chicken produced more (100.80 no/year/chicken) and larger sized (43.21g) eggs than spotted chicken (83.4 no/year/chicken and 40.46g). Among the three...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken; Climatic factors; Microsatellite; Genetic diversity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2018000400731

Symbiotic Efficiency and Genotypic Characterization of Variants of Bradyrhizobium spp. in Commercial Inoculants for Soybeans
Provedor de dados: 90
Autores: Barbosa,Leonardo de Paiva; Costa,Patrícia Freitas; Ribeiro,Paula Rose Almeida; Rufini,Márcia; Guimarães,Amanda Azarias; Moreira,Fatima Maria de Souza.
ABSTRACT High yields obtained from soybean [Glycine max (L.) Merrill], and consequent profits, are related to efficient symbiosis of soybean with nitrogen fixing bacteria of the Bradyrhizobium genus. Inoculation with strains belonging to the Bradyrhizobium japonicum, B. elkanii, and B. diazofficiens species, approved by the Brazilian Ministry of Agriculture (MAPA), is a common practice for this crop. However, variations in the composition of inoculants can change the quality of the product. The aim of this study was to genetically identify and to evaluate the symbiotic efficiency of isolates with cultural characteristics different from those of strains approved by MAPA and which are present in two commercial inoculants for soybean. Isolates obtained from...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Glycine max; 16S rRNA gene; Cultural characterization; Rhizobia; Genetic diversity.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100432

Analysis of the genetic diversity in Metopolophium dirhodum (Walker) (Hemiptera, Aphididae) by RAPD markers
Provedor de dados: 92
Autores: Lopes-da-Silva,Marcelo; Vieira,Luiz Gonzaga Esteves.
The emergence of host-races within aphids may constitute an obstacle to pest management by means of plant resistance. There are examples of host-races within cereals aphids, but their occurrence in Rose Grain Aphid, Metopolophium dirhodum (Walker, 1849), has not been reported yet. In this work, RAPD markers were used to assess effects of the hosts and geographic distance on the genetic diversity of M. dirhodum lineages. Twenty-three clones were collected on oats and wheat in twelve localitites of southern Brazil. From twenty-seven primers tested, only four primers showed polymorphisms. Fourteen different genotypes were revealed by cluster analysis. Five genotypes were collected only on wheat; seven only on oats and two were collected in both hosts. Genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rose-Grain Aphid; Genetic diversity; Host-races; Insect-plant relationships.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262007000100009

Evaluation of Xylella fastidiosa genetic diversity by fAFPL markers
Provedor de dados: 94
Autores: Kishi,Luciano Takeshi; Wickert,Ester; Lemos,Eliana Gertrudes de Macedo.
The first phytopathogenic bacterium with its DNA entirely sequenced is being detected and isolated from different host plants in several geographic regions. Although it causes diseases in cultures of economic importance, such as citrus, coffee, and grapevine little is known about the genetic relationships among different strains. Actually, all strains are grouped as a single species, Xylella fastidiosa, despite colonizing different hosts, developing symptoms, and different physiological and microbiological observed conditions. The existence of genetic diversity among X. fastidiosa strains was detected by different methodological techniques, since cultural to molecular methods. However, little is know about the phylogenetic relationships developed by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: FAFLP; Xylella fastidiosa; Genetic diversity; DNA fingerprinting.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452008000100037

Cucumber mosaic virus resistance and reproductive biology of brazilian melon accessions
Provedor de dados: 94
Autores: Sanabria-Verón,Nadia Carolina; Melo,Cláusio Antônio Ferreira de; Pereira,Jadergudson; Nunes,Glauber Henrique de Sousa; Oliveira,Ohana Luiza Santos de; Corrêa,Ronan Xavier.
Abstract  In melon (Cucumis melo) the resistance to Cucumber Mosaic Virus (CMV) and the knowledge about reproductive characteristics are important for selection of genitors for breeding population. So, the objective was to investigate if different accessions of melon from a Brazilian germplasm bank have variability in resistance to CMV (FNY strain) and polymorphism in resistance loci markers, as well as whether there is genetic diversity on resistance and floral morphology descriptors and reproductive biology, aiming at genetic improvement. The symptoms induced by CMV were evaluated in 52 melon genotypes which were genotyped with three markers of single nucleotide polymorphisms related to CMV resistance. A sample of 24 genotypes was characterized for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Molecular marker; SNP-CAP; Genetic diversity; Floral and reproductive variability; Ward-MLM; CMV.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000500405

CHEMICAL AND PHENOTYPIC DIVERSITY OF MEXICAN PLUMS (Spondias purpurea L.) FROM THE STATES OF GUERRERO AND MORELOS, MEXICO
Provedor de dados: 94
Autores: ASTUDILLO,YANIK IXCHEL MALDONADO; TEJACAL,IRÁN ALIA; NÚÑEZ-COLÍN,ALBERTO CARLOS; HERNÁNDEZ,JAVIER JIMÉNEZ; MARTÍNEZ,VÍCTOR LÓPEZ.
ABSTRACT  Fruits from 86 ecotypes of Mexican plum were harvested from the states of Guerrero and Morelos during the dry season. Of these, 22 were wild ecotypes and 64 were cultivated varieties. Among the variables measured, those with the highest variation coefficients were color, flavor, and mass (&gt; 45%), highlighting the presence of considerable intra-species variability. Cluster analysis separated the 86 accessions into 5 groups, mainly on the basis of color, flavor, length, and mass. Members from the first three groups had red (Group I), yellow (Group II), or purple (Group III) epicarps and higher values of mass (12.2-16 g), length (29.6-33.9 mm), pulp yield (68.8-71.9% ), TSS (11.16-11.52 °Brix) and flavor index (14.5-18.3), making them suitable...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Quality; Canonical discriminant analysis; Cluster analysis; Genetic diversity; Underutilized crops.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000200302

Molecular characterization of papaya genotypes using AFLP markers
Provedor de dados: 94
Autores: Oliveira,Eder Jorge de; Costa,Juliana Leles; Santos,Lucas Ferraz dos; Carvalho,Fabiana Moraes de; Silva,Aline dos Santos; Dantas,Jorge Luiz Loyola.
Due to the low genetic variability reported in the commercial plantations of papaya (Carica papaya L.), the objective of this study was analyze the genetic diversity of 32 genotypes including cultivars, landraces, inbred lines, and improved germplasm using the AFLP technique (Amplified Fragment Length Polymorphism). The genetic distance matrix was obtained using the Nei and Li genetic distance and clustering was performed using the unweighted pair-method with arithmetic mean (UPGMA). Using 11 combinations of EcoRI/MseI primers, 383 polymorphic bands were obtained. On average, 34.8 polymorphic bands were obtained per primer combination. Five clusters were formed. The traditional cultivar 'Sunrise' and the inbred line CMF-L30-08 were the closest genotypes,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Papaya breeding; Genetic resources; Genetic diversity; Carica papaya L.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452011000300020

Genetic variations among passion fruit species using rapd markers
Provedor de dados: 94
Autores: Aukar,Ana Paula de Andrade; Lemos,Eliana Gertrudes de Macedo; Oliveira,João Carlos.
It has been evaluated the genetic variability through the use of RAPD molecular markers on the following passionflower species: Passiflora amethystina, P. caerulea, P. cincinnata, P. coccinea, P. serrato digitata, P. foetida, P. maliformis, P. alata, P. giberti, P. laurifolia, P. macrocarpa, P. nitida, P. setacea, P. suberosa, P. ligularis, P. capsularis, P. edulis Sims and its botanical variety P. edulis Sims f. flavicarpa Deg. In this research work, the analyses of the random amplified polymorphic DNA products (RAPD) were employed to estimate the genetic diversity and the taxonomic linkage within the species above. The total of 21 primers were used in this study which generated 270 different polymorphic products. It was possible to detect that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: DNA; Genetic diversity; Passion fruit; Random Amplified Polymorphic DNA.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000300044

Evaluation of SSR and SNP markers in Rubus glaucus Benth progenitors selection
Provedor de dados: 94
Autores: López,Ana María; Barrera,Carlos Felipe; Marulanda,Marta Leonor.
Abstract Rubus glaucus Benth (known as “mora de castilla”) is a Colombian agricultural product, with probably, the major potential. This fruit combines features of Idaeobatus and Rubus subgenera. Despite its recognized importance in the economy of small producers, this crop has received little technological development; as a result, sowing procedures of this specie is done by using local cultivars asexually propagated by producers. Associated Rubus producers in Colombia has noticed the necessity to formalize the offer of planting material, starting with plant breeding programs tending to the obtention of more productive varieties with morphological features that facilitates cultural activities. This study presents the results of the evaluation of SSR and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant breeding; Andean blackberry; Genetic diversity.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000100803

POPULATION GENETIC ANALYSIS OF BRAZILIAN PEACH BREEDING GERMPLASM
Provedor de dados: 94
Autores: THUROW,LIANE BAHR; RASEIRA,MARIA DO CARMO BASSOLS; BONOW,SANDRO; ARGE,LUIS WILLIAN PACHECO; CASTRO,CAROLINE MARQUES.
ABSTRACT  Peach has great economic and social importance in Brazil. Diverse sources of germplasm were used to introduce desirable traits in the Brazilian peach breeding pool, composed mainly by local selections and accessions selected from populations developed by the national breeding programs, adapted to subtropical climate, with low chill requirement, as well as accessions introduced from several countries. In this research, we used SSR markers, selected by their high level of polymorphism, to access genetic diversity and population structure of a set composed by 204 peach selected genotypes, based on contrasting phenotypes for valuable traits in peach breeding. A total of 80 alleles were obtained, giving an average of eight alleles per locus. In...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus persica; Genetic diversity; Population structure; Low chill germplasm; Genetic resources.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452017000500802

Diversity and genetic struture in cajá tree (spondias mombin l.) populations in northeastern Brazil
Provedor de dados: 94
Autores: Silva,Edson Ferreira da; Martins,Luiza Suely Semen; Oliveira,Visêldo Ribeiro de.
Spondias mombin L. is a fruit tree from the American continent from the Anacardiaceae family. In Brazil it is common in different vegetation types but is more frequent in the Atlantic and Amazonian rainforests. It is economically important because of its fruits, which are widely consumed raw or processed as fruit jellies, juices and ice creams. The leaves have great importance in the pharmaceutical industry because of their antibacterial properties. In the state of Pernambuco, cajá tree is widely distributed in the Zona da Mata region and less frequently in the Agreste and Sertão areas. In this work diversity and genetic structure were studied in four populations of cajá tree from Pernambuco's Zona da Mata, Northeast Brazil, using isozymes polymorphism...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Germplasm conservation; Isozymes.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452009000100024

Morpho-genetic profiling and phylogenetic relationship of guava (Psidium guajava l.) as genetic resources in Pakistan
Provedor de dados: 94
Autores: Kareem,Abdul; Jaskani,Muhammad Jafar; Mehmood,Asim; Khan,Iqrar Ahmad; Awan,Faisal Saeed; Sajid,Muhammad Wasim.
Abstract  Guava (Psidium guajava L.) is an open-pollinated crop having 25-40% dissimilarity index which promotes heterozygosity and adds new cultivars. Morpho-genetic characterization of 37 guava accessions was carried out for genetic variability and structure of guava germplasm located in Punjab province, Pakistan. Principal Component analysis (PCA) was subjected to analyze the morphological diversity and for genetic analysis we applied cluster analysis, using the PyElph software. PCA distributed thirty one traits into six components and first two components accounted 39.5% of total variation. A dendrogram constructed on the basis of morphological traits which showed 34% dissimilarity index among thirty seven guava accessions and divided them into 6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross pollinated; Dendrogram; Genetic diversity; Reproducible; SSR markers.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452018000400801

MOLECULAR CHARACTERIZATION OF JABOTICABA TREE GENOTYPES LOCATED IN THE MUNICIPALITIES OF RECÔNCAVO OF BAHIA
Provedor de dados: 94
Autores: CRUZ,ELAINE SILVA DA; DANTAS,ANA CRISTINA VELLO LOYOLA; CARMO,CÁTIA DIAS DO; BASTOS,LUCIMÁRIO PEREIRA.
ABSTRACT  The jaboticaba tree (Plinia sp.) is a native fruit tree belonging to the Myrtaceae family, which occurs spontaneously throughout Brazil. The estimation of genetic divergence among genotypes of native populations can be useful for conservation and knowledge of available genetic resources, in the formation of gene banks and breeding species. In order to evaluate the genetic diversity of jaboticaba trees identified in the Bahia Reconcavo municipalities, 35 genotypes were characterized by ISSR primers (Inter Simple Sequence Repeats). The 18 primers generated a total of 463 amplicons, and the number of initiator fragments ranged from 14 to 36, with an average of 25.72% and 99.65% polymorphism. Polymorphic information content ranged from 0.13 to 0.33...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; ISSR; Plinia sp.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452016000300803

Genetic monitoring of a Santa Ines herd using microsatellite markers near or linked to the sheep MHC
Provedor de dados: 96
Autores: Petroli,César Daniel; Paiva,Samuel Rezende; Corrêa,Marilma Pachêco Chediak; McManus,Concepta.
This study aimed to analyze genetic diversity in a conservation nucleus of Santa Inês sheep using thirteen microsatellite loci on chromosome 20 (where the Sheep Major Histocompatibility Complex - Ovar-MHC - is found). Seventy three animals from one herd born from 2004 to 2006 were evaluated as a principal nucleus. Seventy one animals from two other herds were used as control comparison. There was a reduction in heterozygosity over the years in relation to the whole population. This may be due to the repeated use of the same sires. The estimates of molecular coancestrality also indicated an increase in genetic similarity between individuals with the herd over the years. A high number of alleles occurred exclusively in the principal nucleus herd, but with a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Animal genetic resources; Genetic diversity; Ovar-MHC; Ovis aries; Molecular coancestrality; SSR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982009000400012

Genetic diversity of four cattle breeds using microsatellite markers
Provedor de dados: 96
Autores: Machado,Marco Antonio; Schuster,Ivan; Martinez,Mário Luiz; Campos,Ana Lúcia.
Four cattle breeds (Gyr, Nellore, Guzerat and Holstein) were analyzed by amplification of genomic DNA using microsatellite loci to evaluate the genetic diversity within and among them. DNA samples of 18 animals from each breed were collected to access the genetic content of them. Allele frequencies were calculated and used to generate a Nei's genetic distance matrix what was used to build a dendrogram following UPGMA clustering. As expected, Holstein breed was the most distinct from the other breeds: 1.15 in relation to Gyr, 1.12 in relation to Nellore and 0.94 in relation to Guzerat. The closest genetic distance was 0.25 between Guzerat and Nellore. A total of 64 alleles in all four breeds were detected using nine microsatellite primers. Each breed showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; DNA; Genetic diversity; Marker; Microsatellite.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982003000100012

Genetic variability of tambaqui broodstocks in the Brazilian state of Pará
Provedor de dados: 96
Autores: Ferreira,Leandro de Araújo; Fazzi-Gomes,Paola Fabiana; Guerreiro,Sávio; Rodrigues,Marília Danyelle Nunes; Ribeiro-dos-Santos,Ândrea Kely; Santos,Sidney; Hamoy,Igor.
ABSTRACT The present study evaluated the genetic variability of the broodstocks of tambaqui hatcheries in the Brazilian state of Pará. Samples were obtained from the caudal fin of 110 broodstocks from one hatchery in each of four municipalities (Santarém, Peixe-Boi, Breu-Branco, and Ulianópolis), representing all the individuals of each hatchery stock. The samples were genotyped using the multiplex system microsatellite markers. Data were used to calculate observed and expected heterozygosity, number of alleles per locus, and allelic richness. The effective population size and inbreeding coefficient were also calculated. The genetic differentiation between populations was evaluated by using FST, and population structuring by the most likely number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Colossoma macropomum; Fish; Genetic diversity; Hatcheries.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100302

Genetic diversity of the Brazilian Creole cattle Pé-duro assessed by microsatellites and mitochondrial DNA
Provedor de dados: 96
Autores: Oliveira,Ana Paula Ferreira de; Carvalho,José Herculano de; Miretti,Marcos Mateo; Lara,Maria Aparecida Cassiano; Contel,Eucleia Primo Betioli.
The objective of this study was to describe the genetic diversity and structure of the largest Pé-duro population by assessing variation at ten autosomal microsatellite (STR) loci and mitochondrial DNA (mtDNA) sequences. The mean expected heterozygosity was 0.755, the mean observed heterozygosity was 0.600 and significant inbreeding coefficient (Fis) and deviations from the Hardy-Weinberg equilibrium in most of analyzed loci demonstrate the impact of inbreeding and homozygosis on this population. A more in-depth genetic analysis could be achieved by expanding the STR list. The analysis of mtDNA provided evidence of ancestral African taurine haplotypes in Pé-duro and excluded maternal Zebuine introgression. In this report, the main Pé-duro population is...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bos taurus; Cattle; Conservation; Genetic diversity; Microsatellites; Mitochondrial DNA.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001100003

Genetic variability of Pantaneiro horse using RAPD-PCR markers
Provedor de dados: 96
Autores: Egito,Andréa Alves do; Fuck,Beatriz Helena; McManus,Concepta; Paiva,Samuel Rezende; Albuquerque,Maria do Socorro Maués; Santos,Sandra Aparecida; Abreu,Urbano Gomes Pinto de; Silva,Joaquim Augusto da; Sereno,Fabiana Tavares Pires de Souza; Mariante,Arthur da Silva.
Blood samples were collected from Pantaneiro Horses in five regions of Mato Grosso do Sul and Mato Grosso States. Arabian, Mangalarga Marchador and Thoroughbred were also included to estimate genetic distances and the existing variability among and within these breeds by RAPD-PCR (Random Amplified Polymorphic DNA - Polymerase Chain Reaction) molecular markers. From 146 primers, 13 were chosen for amplification and 44 polymorphic bands were generated. The analysis of molecular variance (AMOVA) indicated that the greatest portion of detected variability was due to differences between individuals within populations (75.47%). Analysis of the genetic variability between pairs of populations presented higher estimates for the five Pantaneiro populations with the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AMOVA; Arabian; English Thoroughbred; Genetic diversity; Genetic resource; Mangalarga Marchador.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982007000400007

Effect of host-plant genetic diversity on oak canopy arthropod community structure in central Mexico
Provedor de dados: 97
Autores: Tovar-Sánchez,Efraín; Valencia-Cuevas,Leticia; Mussali-Galante,Patricia; Ramírez-Rodríguez,Rolando; Castillo-Mendoza,Elgar.
BACKGROUND: Recently it has been proposed that the genetic diversity of foundation species influences the structure and function of the community by creating locally stable conditions for other species and modulating ecosystem dynamics. Oak species are an ideal system to test this hypothesis because many of them have a wide geographical distribution, and they are dominant elements of the forest canopy. In this study we explored the response of canopy arthropod community structure (diversity and biomass) to the level of genetic diversity of Quercus crassipes and Q. rugosa, two important canopy species. Also, we examined the effect of oak species and locality on some community structure parameters (diversity, biomass, rare species, and richness of arthropod...
Tipo: Journal article
Palavras-chave: Arthropods community; Canopy; Foundation species; Genetic diversity; Quercus.
Ano: 2015
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-078X2015000100012

Determination of the genetic variation in Ajowan (Carum Copticum L.) populations using multivariate statistical techniques
Provedor de dados: 124
Autores: Dalkani,Mahdieh; Hassani,Abbas; Darvishzadeh,Reza.
The experiment was conducted at Urmia University, to assess genetic diversity in populations of Iranian Ajwain based on agronomical and morphological characteristics. Ten populations, collected from different regions, were evaluated in a completely randomised design with eight to ten replications. Various agronomical and morphological characteristics were measured, including stem diameter, plant height, number of branches, number of leaves and leaflets, number of umbels, number of umbellules per inflorescence, number of flowers per umbellule with inflorescence, leaf length, average internodal distance, shoot dry matter, one hundred seed weight, number of seeds, biological production, single plant yield, length of ripening period and harvest index. Among...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ajwain; Genetic diversity; Heritability.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902012000400011

Genetic variation in oriental tobacco (Nicotiana tabacum L.) by agro-morphological traits and simple sequence repeat markers
Provedor de dados: 124
Autores: Darvishzadeh,Reza; Mirzaei,Leila; Maleki,Hamid Hatami; Laurentin,Hernan; Alavi,Seyyed Reza.
The objectives of this study were to assess genetic diversity and determine differences between several oriental tobacco genotypes by examining both agro-morphological traits and molecular markers. Simple lattice design with two replications was used to evaluate 100 oriental tobacco genotypes. Analysis of variance manifested that there is high level of genetic diversity in oriental-type tobaccos based on morphological traits including number of leaf, days to 50% flowering, leaf length, leaf width, leaf fresh weight, leaf dry weight, stem height and stem girth. Classification of genotypes using agro-morphological data by means of un-weighted pair-group method using arithmetic average (UPGMA) algorithm based on squared standardized Euclidean distances...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster analysis; Euclidean distance; Genetic diversity; Jaccard's similarity; Oriental tobacco.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902013000200018

Proposal for qualitative and quantitative descriptors to characterise bamboo germplasm
Provedor de dados: 124
Autores: Generoso,Andressa Leal; Santos,Jardel Oliveira; Carvalho,Virginia Silva; Sacoman,Nayara Niele; Rodrigues,Rosana.
ABSTRACT Bamboo is a genetic resource with evident potential for use from construction until the recovery of degraded areas. Although, characterization and evaluation studies involving bamboo species are scarce and it is difficult to define a list of specific descriptors to better meet the different genres and or bamboo species. Thus, the objective of this work were propose and test the effectiveness of qualitative and quantitative descriptors for phenotypic characterization and the study of genetic diversity among six species of bamboo introduced in Brazil. Fifteen qualitative and nine quantitative descriptors were proposed and tested in clones with one year of planting. Individuals belonging to six species of bamboo (Bambusa vulgaris, Bambusa vulgaris...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bambusa; Drepanostachyum; Dendrocalamus; Phyllostachys; Genetic diversity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000100047

Characterization of typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the classical O55 serogroup by RAPD analysis
Provedor de dados: 59
Autores: Girão,Dennys M.; Bando,Sílvia Y.; Girão,Valéria Brígido de C.; Moreira-Filho,Carlos A.; Fracalanzza,Sérgio Eduardo L.; Trabulsi,Luiz R.; Monteiro-Neto,Valério.
The genetic diversity of 41 typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the serogroup O55 was analyzed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to the serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on E. coli O55 infection.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Genetic diversity; RAPD analysis.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400013

Morphological variation and isozyme diversity in Dioscorea alata L. landraces from Vale do Ribeira, Brazil
Provedor de dados: 63
Autores: Bressan,Eduardo de Andrade; Briner Neto,Thiago; Zucchi,Maria Imaculada; Rabello,Ronaldo José; Veasey,Elizabeth Ann.
Traditional growers of the Vale do Ribeira, São Paulo State, grow and make use of several D. alata landraces. This study assessed the genetic diversity of 16 landraces, using isozymatic and morphological markers and comparing them with 19 commercial varieties of D. alata. Their distribution in different levels of organization such as households and communities of the Vale do Ribeira was evaluated. Isozymatic analyses were performed with polyacrylamide (six systems) and starch gels (one system), while the morphological analyses were carried out with 24 traits. Due to the polyploid nature of this species, the isozymatic bands were scored as binary data. Morphological traits were also scored as binary data. Principal coordinates and cluster analyses were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AMOVA; Traditional agriculture; Genetic diversity; Water yam.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162011000400016

A lima bean core collection based on molecular markers
Provedor de dados: 63
Autores: Gomes,Regina Lucia Ferreira; Costa,Marcones Ferreira; Alves-Pereira,Alessandro; Bajay,Miklos Maximiliano; Viana,João Paulo Gomes; Valente,Sérgio Emílio dos Santos; Lopes,Ângela Celis de Almeida; Zucchi,Maria Imaculada; Pinheiro,José Baldin.
ABSTRACT Some germplasm collections have a high number of accessions, which makes it difficult to explore the genetic variability present in the germplasm bank due to the redundancy and the difficulty of detailed analysis of all conserved accessions. Therefore, our study aimed to analyze the genetic diversity of 153 lima bean (Phaseolus lunatus) accessions for the purpose of constructing a core collection. Eleven SSRs were used for this purpose. The 153 lima bean accessions can be represented by low redundancy using a minimum of 34 accessions, thus representing 22 % of the size of the entire germplasm bank. The core collection had a higher Shannon diversity index and expected heterozygosity (1.906 and 0.811, respectively) than those presented by the entire...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm; Genetic diversity; Genetic structure; Microsatellite markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000201100

Diagnostic fingerprints ISSR/SSR for tropical leguminous species Stylosanthes capitata and Stylosanthes macrocephala
Provedor de dados: 63
Autores: Alzate-Marin,Ana Lilia; Costa-Silva,Carolina; Rivas,Priscila Marlys Sá; Bonifacio-Anacleto,Fernando; Santos,Leticia Gobett; Moraes Filho,Rômulo Maciel de; Martinez,Carlos Alberto.
ABSTRACT: Stylosanthes capitata Vogel and Stylosanthes macrocephala M.B.Ferreira &amp; Sousa Costa are two forage leguminous species of agronomic importance for animal husbandry in tropical environments. The physical mixture of both species (80 % S. capitata and 20 % S. macrocephala) comprises the commercial cultivar &amp;#8220;Estilosantes Campo Grande”. However, proximity of fields for seed production may contaminate seed lots, compromising seeds quality. The combined use of dominant and co-dominant molecular markers is an appropriate strategy to certificate genetic purity and perform diversity studies of cultivars. In this research, a set of ISSR (Inter-Simple Sequence Repeat polymorphic DNA) and SSR (Simple Sequence Repeat polymorphic DNA) molecular...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic purity; Genetic diversity; Seed production.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000301101

Identification and validation of novel EST-SSR markers in olives
Provedor de dados: 63
Autores: Arbeiter,Alenka Baruca; Hladnik,Matjaž; Jakše,Jernej; Bandelj,Dunja.
ABSTRACT: The olive (Olea europaea L.) is a leading oil crop in the Mediterranean area. Limited information on the inheritance of agronomic significant traits hinders progress in olive breeding programs, which encourages the development of markers linked to the traits. In this study, we report on the development of 46 olive simple sequence repeat (SSR) markers, obtained from 577,025 expressed sequence tags (ESTs) in developing olive fruits generated in the framework of the Slovenian national olive transcriptome project. Sequences were de novo assembled into 98,924 unigenes, which were then used as a source for microsatellites searching. We identified 923 unigenes that contained 984 SSRs among which dinucleotide SSRs (36 %) were the most abundant, followed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Expressed sequence tags; Microsatellites; Genic markers; Genetic diversity; Functional annotation.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162017000300215

Influence of natural and synthetic carotenoids on the color of egg yolk
Provedor de dados: 63
Autores: Spada,Fernanda Papa; Selani,Miriam Mabel; Coelho,Antonio Augusto Domingos; Savino,Vicente José Maria; Rodella,Arnaldo Antônio; Souza,Miriam Coelho; Fischer,Flavia Salgado; Lemes,Dayane Elizabethe Aokui; Canniatti-Brazaca,Solange Guidolin.
ABSTRACT Carotenoids are incorporated into the diet of laying hens in order to modify the yolk color. A natural source of carotenoids in tropical countries is annatto, which could be used in the diets of hens. This study aimed to evaluate the addition of natural (annatto) and synthetic carotenoids to the diet of laying hens (commercial and alternative) and their effects on yolk color and consumer sensory perception of fresh and stored eggs obtained from two different preparations (boiled and fried). Physicochemical analysis of proximate composition, thiobarbituric acid reactive substances (TBARS), emulsion activity and instrumental color were performed. Cooking caused significant alterations to the moisture in the preparations and this may have directly...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bixa orellana; Prepared foods; Sensory evaluation; Genetic diversity.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162016000300234

Phenotypic and molecular characterization of apple (Malus × domestica Borkh) genetic resources in Greece
Provedor de dados: 63
Autores: Ganopoulos,Ioannis; Tourvas,Nikolaos; Xanthopoulou,Aliki; Aravanopoulos,Filippos A.; Avramidou,Euaggelia; Zambounis,Antonios; Tsaftaris,Athanasios; Madesis,Panagiotis; Sotiropoulos,Thomas; Koutinas,Nikolaos.
ABSTRACT: The phenotypic and genetic analysis of apple (Malus × domestica Borkh) genotypes is essential for breeding species. Information on the morphology and genetic structure of apple offers significant help for germplasm maintenance and selection of suitable material to breed superior cultivars. This study shows the results of an investigation on the morphology and the genetic diversity for 19 apple cultivars, which are preserved in an ex situ collection in Naoussa, Central Macedonia, Greece. Information was recorded over a 5-year period for 47 traits describing plant morphology and phenotype, as well as leaf and fruit quality. Data were analyzed using the principal component analysis (PCA) method. The Euclidean distance metric and the Ward’s...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Genotyping; Microsatellite markers; Multivariate analysis; Phenotyping.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162018000600509

Genetic structure of annual ryegrass (Lolium multiflorum) populations estimated by RAPD
Provedor de dados: 63
Autores: Vieira,Eduardo Alano; Castro,Caroline Marques; Oliveira,Antônio Costa de; Carvalho,Fernando Irajá Félix de; Zimmer,Paulo Dejalma; Martins,Luís Fernando.
Annual ryegrass is a temperate climate annual foraging grass, grown mostly in the South of Brazil, especially in the state of Rio Grande do Sul. Despite its importance, little is known about its genetic diversity, both within and among the populations cultivated. This knowledge is of fundamental importance for developing breeding and conservation strategies. The aim of this study was to characterize the genetic diversity and structure of four populations of annual ryegrass. Three of the populations were located in Rio Grande do Sul and the fourth in Uruguay. RAPD markers were used to study the genetic diversity and structure of these populations. Analysis of 375 individuals sampled from the populations, using six RAPD primers, generated a total of 82...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Foraging; Genetic diversity; Molecular markers.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400009

Old tools as new support for on farm conservation of different types of maize
Provedor de dados: 63
Autores: Vidal,Rafael; Silva,Natália Carolina de Almeida; Ogliari,Juliana Bernardi.
ABSTRACT: A germplasm collection should represent the diversity of the target species and the gene pools associated with it. However, it is critical to establish collection plans which ensure such representativeness. At times it is difficult to identify the best strategy for collecting domesticated species that are conserved in situ/on farm, since, in general, the magnitude of the diversity existing in a geographical area was hitherto unknown. The Diversity Census methodology was developed for previous diagnosis of the diversity of Zea mays subsp. mays L., conserved by farmers in two municipalities in the far western region of the state of Santa Catarina, southern Brazil. The Diversity Census database allowed for the identification of the best strategy to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays L.; Genetic diversity; Morphological characters; Use values.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000101100

Development of an SSR-based identification key for Tunisian local almonds
Provedor de dados: 63
Autores: Gouta,Hassouna; Ksia,Elhem; Buhner-Zaharieva,Tatiana; Mliki,Ahmed; Gogorcena,Yolanda.
Ten simple sequence repeat (SSR) loci were used to study polymorphism in 54 almond genotypes. All genotypes used in this study originated from almond-growing areas in Tunisia with different climatic conditions ranging from the sub-humid to the arid and are preserved in the national collection at Sidi Bouzid. Using ten SSR, 130 alleles and 250 genotypes were revealed. In order to develop an identification key for each accession, the data were analysed separately for each microsatellite marker. The most polymorphic microsatellite (CPDCT042) was used as a first marker. Two microsatellite loci (CPDCT042 and CPDCT025) were sufficient to discriminate among all accessions studied. Neighbour-joining clustering and principal coordinate analysis were performed to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prunus dulcis Mill; Genetic diversity; Genetic resources; Microsatellites; Molecular characterization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200004

Microsatellite diversity and chromosome number in natural populations of Trifolium riograndense Burkart
Provedor de dados: 63
Autores: Conterato,Ionara Fatima; Dall'Agnol,Miguel; Schifino-Wittmann,Maria Teresa; Janke,Aline.
Twenty eight natural populations of Trifolium riograndense Burkart, an important forage legume from native pastures of the state of Rio Grande do Sul, Brazil, were evaluated for genetic diversity with eight Simple Sequences Repeats (SSR) markers. Chromosome numbers were also determined. The eight markers were polymorphic, with 35 alleles and an average of 4.37 alleles per locus, and Polymorphism Information Content (PIC) between 0.48 and 0.80. Group analysis based on Jaccard´s similarity coefficient separated the 28 accessions in nine groups, with an average genetic similarity of 0.44, indicating a high genetic variability among the populations. No evident relation between genetic distance and geographical origin was detected. The chromosome number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Native clover; Genetic diversity; Cytogenetics; Native pastures.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200005

Genetic similarity among Tunisian cultivated olive estimated through SSR markers
Provedor de dados: 63
Autores: Abdelhamid,Sofiane; Grati-kamoun,Naziha; Marra,Francesco; Caruso,Tiziano.
Olive (Olea europaea L. subsp. europaea var. europaea) is one of the oldest fruit tree in the Mediterranean basin, and is cultivated for oil and canned fruit. Part of this interest is driven by the economic importance of olive oil which is increasing throughout the world due to its beneficial effect to human health. In Tunisia, olive has great socio-economic importance, with more than 60 millions olive trees cultivated for olive oil production including a wide range of cultivars which are widely extended from the north to the south regions of the country for its high economic value. Here, we applied microsatellites (SSRs) molecular markers to assess the genetic variability of the most important Tunisian olive cultivars. In total, the 10 simple sequence...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Olea europaea; Genetic diversity; Microsatellite; Genetic relationships.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162013000100006

Biological and molecular characterization of Brazilian isolates of Zucchini yellow mosaic virus
Provedor de dados: 63
Autores: Spadotti,David Marques de Almeida; Wassano,Débora Targino; Rezende,Jorge Alberto Marques; Camargo,Luis Eduardo Aranha; Inoue-Nagata,Alice Kazuko.
Zucchini yellow mosaic virus (ZYMV) causes substantial economic losses in cucurbit crops. Although ZYMV has been present in Brazil for more than 20 years, there is little information about the biological and molecular characteristics of the isolates found in the country. This study aimed to characterize the experimental hosts, pathotypes and genetic diversity of a collection of eleven Brazilian ZYMV isolates within the coat protein gene. For biological analysis, plant species from Amaranthaceae, Chenopodiaceae, Cucurbitaceae, Fabaceae, Solanaceae, and Pedaliaceae were mechanically inoculated and pathotypes were identified based on the reaction of a resistant Cucumis melo, accession PI414723. All of the cucurbit species/varieties and Sesamum indicum were...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Potyvirus; Nucleotide sequence; Genetic diversity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162015000200187

Sorghum breeding
Provedor de dados: 106
Autores: Krisda Samphantharak; Wasana Wongyai; Sutee Rayakaew; Chaba Amramphai; Thamrongsilpa Pothisoong; Prapon Boonrumpun.
1 ill., 6 tables
Palavras-chave: Sorghum; Breeding; Yields; Adaptability; Early maturity; Genetic diversity; Backcross; Population; ข้าวฟ่าง; การปรับปรุงพันธุ์; ผลผลิต; การปรับตัว; พันธุ์เบา; การผสมกลับ; การปรับปรุงประชากร.
Ano: 1985
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4566

การจำแนกความหลากหลายของเชื้อพันธุกรรมข้าวด้วยลักษณะสัณฐานวิทยา
Provedor de dados: 106
Autores: Kittima Ruksopa; Prachack Lengbumrung; Perapol Muangngam.
Classification of rice genetic diversity aims to study the genetic relationship and reduce the duplication of collected rice varieties in the National Rice Seed Storage Laboratory for Genetic Resources.  Cluster analysis of the morphological and agronomic traits of 211 rice varieties was calculated by Simple Matching and Square Euclidean as well as clustered by UPGMA and Ward method, respectively.  Cluster based on morphology having 6 main groups indicated that the highest number of members within the group was Group 1 consisting of 137 rice varieties, followed by Group 2, 3, 15, 6 and 9 consisting of 16, 12, 6, 5 and 3 rice varieties, respectively.  Cluster by agronomic traits having 9 main groups showed that the highest number of members was Group3...
Tipo: PhysicalObject
Palavras-chave: Rice; Genetic diversity; Cluster analysis; Morphological traits; Coefficient similarity; ข้าว; เชื้อพันธุกรรม; การวิเคราะห์กลุ่ม; ลักษณะสัณฐานวิทยา; สัมประสิทธิ์ความเหมือน; ลักษณะทางการเกษตร.
Ano: 2014
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5638

ความหลากหลายและสัมพันธ์ทางพันธุกรรมระหว่างอ้อยพันธุ์การค้าในประเทศไทย
Provedor de dados: 106
Autores: Chaba Thongpaiyai; Anucha Wongpraneekul; Prasert Chatwachirawong.
Objective of this study was conducted to determine the diversity and relationships among commercial sugarcane varieties in Thailand. The pedigree in each variety was traced back to the original ancestors. The coefficient of parentage was calculated from records of 70 varieties, and a dendrogram was drawn using the unweighted pair-group method using arithmetic averages (UPGMA) of clustering. Pedigree data showed that Thai commercial varieties were only bred from 26 exotic varieties. The analytical results indicated that many sugarcane varieties had higher close relationship with OCSB3 (37.3%) than UT1 (20.4%). S. officinarum was major genetic contributor (25.1%) whereas S. barberi, S. spontaneum, and S. sinense were 10.8, 5.9, and 2.4%, respectively. These...
Tipo: Collection
Palavras-chave: Sugarcane breeding; Coefficient of parentage; Commercial sugarcane; Genetic diversity; อ้อย; การปรับปรุงพันธุ์; สัมประสิทธิ์ทางเครือญาติ; พันธุ์การค้า; ความหลากหลายทางพันธุกรรม; ประเทศไทย.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5313

การปฏิวัติยีน กับการปรับปรุงพันธุ์พืช
Provedor de dados: 106
Palavras-chave: Gene revolution; Plant improvement; Biodiversity; Genetic diversity; Species diversity; Eco-system diversity; Mutation; Genome research; การปรับปรุงพันธุ์พืช; การปฏิวัติยีน; การแสดงออกของยีน; พันธุวิศวกกรม; การตัดต่อยีน; จีเอ็มโอ; ไลเปส; ความปลอดภัยทางชีวภาพ; การติดฉลาก.
Ano: 2001
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/4156

ความหลากหลายทางพันธุกรรมและโครงสร้างประชากรของข้าววัชพืชในประเทศไทย
Provedor de dados: 106
Autores: Anupong Wongtamee; Chanya Maneechote; Benjavan Rerkkasem; Sansanee Jamjod.
Weedy rice (Oryza sativa f. spontanea) is one of most serious weeds in direct-seeded rice production. High genetic diversity and adaptability of weedy rice made it very difficult to control in the rice field. This study aim to assess genetic diversity and population structure of weedy rice populations from Lower North, Northeast and Central of Thailand in wet of 2009 using twelve microsatellite markers. Genetic diversity and population structure of weedy rice were determined and compared with seven common wild rice populations and seven check varieties commonly grown in these regions. Weedy rice populations in this present study were hybrid between common wild rice and crop rice. The population structure of them was structured into 2 clusters according to...
Tipo: Collection
Palavras-chave: Weedy rice; Genetic diversity; Population structure; Gene flow; ข้าววัชพืช; ความหลากหลายทางพันธุกรรม; โครงสร้างประชากร; การแลกเปลี่ยนยีน; ประเทศไทย.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5333

ความหลากหลายทางพันธุกรรมของเชื้อไฟโตพลาสมาที่ก่อโรคในอ้อย และหญ้าบางชนิดของประเทศไทย จากลำดับนิวคลีโอไทด์บริเวณ 16S-23S rRNA intergenic spacer region
Provedor de dados: 106
Autores: Suchirat Sakuanrungsirikul; Theerawut Wongwarat; Sunee Srisink; Piyada Theerakulpisut.
The phylogenetic analysis of phytoplasmas focused on the nucleotide sequences of the intergenic 16s-23s rRNA spacer region that primers enable the amplification of a 210 bp fragment by nested-PCR, are established for studying of genetic diversity of phytoplasma associated with diseases of sugarcane and some grasses in Thailand. In this present study, 99 phytoplasma infected sugarcane samples exhibiting various phenotypic symptoms and 15 samples of grasses showing white leaves were collected from many locations in Thailand and subjected for nucleotide sequencing. Phylogenetics tree based on 16S-23S ITS gene sequences showed two primary clusters: (1) phytoplasmas that cause diseases in sugarcane and (2) phytoplasmas that cause diseases in grasses. The...
Tipo: Collection
Palavras-chave: Phytoplasma; Sequencing analysis; White leaves diseases; Genetic diversity; อ้อย; โรคใบขาว; ไฟโตพลาสมา; เชื้อสาเหตุโรค; ลำดับนิวคลีโอไทด์; ความหลากหลายทางพันธุกรรม.
Ano: 2012
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5551

ความผันแปรทางพันธุกรรมของกุ้งก้ามกรามโดยเทคนิคไมโครแซทเทลไลท์
Provedor de dados: 106
Autores: Teera Patthanajak; Siripavee Charoenwattanasak; Monchai Duangjinda; Yupin Phasook.
Objective of this study was to study the genetic diversity in Giant Freshwater Prawn in Thailand from 4 locations, which were Khon Kaen (KK), Samutsongkhram (SK), Suratthani (SR) and Angthong (AT), with a total of 100 samples. Microsatellite technique with 13 primers and separating on 6% denaturing polyacrylamide gel were used in this study. The result showed that polymorphic loci had 105 alleles, the average number of alleles per locus was 8.08. The frequency of alleles ranged from 0.00 to 0.88. The average expected heterozygosity at all loci was 0.73 and the genetic distance ranged from 0.15 to 0.20, showed that the population from Samutsongkhram and Angthong were genetic the closest, while the population from Samutsongkhram and Suratthani were the most...
Tipo: Collection
Palavras-chave: Genetic diversity; Giant Freshwater Prawn; Microsatellite technique; กุ้งก้ามกราม; ไมโครแซทเทลไลท์; ความหลากหลายทางพันธุกรรม; ดีเอ็นเอ.
Ano: 2011
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5191

ความหลากหลายทางพันธุกรรมและชีวชนิดของแมลงบั่วในประเทศไทย
Provedor de dados: 106
Autores: Jirapong Jairin; Kanlaya Sansen; Sa-nguan Teangdeerith; Phikul Leelakud; Rungnapa Kawichai; Wantana Siratanasak; Nalinee Chiengwattana.
Amplified fragment length polymorphism (AFLP) and biotype-specific markers were used for identification of genetic diversity and biotypes of rice gall midge, Orseolia oryzae in Thailand. Pupae and adults of the gall midges were collected from 19 field locations of 12 provinces during 2006 - 2007. Total of 242 polymorphic AFLP bands were generated from 12 selective primer pairs. Cluster analysis, performed by the UPGMA, separated the gall midge populations into four distinct groups. To identify the biotype, five specific-biotype markers were used to characterize the collected gall midges. Two biotypes of the gall midges, biotype 2 and 5, were identified from all populations used in this research. The results showed that the genetic diversity was not...
Tipo: Collection
Palavras-chave: Orseolia oryzae; Rice gall midge; Biotype; Genetic diversity; AFLP; ข้าว; แมลงบั่ว; การจำแนกชีวชนิด; ความหลากหลายทางพันธุกรรม; เอเอฟแอลพี; โมเลกุลเครื่องหมาย; ประเทศไทย.
Ano: 2009
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5092

การประเมินความแปรปรวนลักษณะทางสัณฐานวิทยา และความหลากหลายทางพันธุกรรมของข้าวนาที่สูง
Provedor de dados: 106
Autores: Parinyaporn Muangpil; Supinya Khantipap; Sukarkarn Sriboon; Naras Sirigasorn; Ruangchai Juwattanasomran; Sivapong Naruban; Pornpan Pooprompan.
Ninety-three samples of high land rice, “Bue Po Lo” variety from Mae Hong Son province and one sample of “Eto” variety from Chiang Rai province were evaluated for genetic diversity at Maejo University during June to December of 2012. Treatments were arranged in a Randomized Complete Block design with 2 replications. Cluster analysis of quality characters using NTSYS software was performed to construct dendrogram. The results found that sheath color, internode color, awn character, stem pattern, flag leaf pattern, panicle base pattern, stem vigor, secondary branch pattern, panicle pattern, panicle exertion and threshing were significantly different. While, leaf pubescence, ligule pattern and stigma color were not significantly different. Cluster analyses of...
Tipo: PhysicalObject
Palavras-chave: Highland rice; Genetic diversity; Morphological characters; Bue Po Lo; Eto; ข้าว; พันธุ์บือโป๊ะโละ; พันธุ์ข้าวอิโต 1; ความหลากหลายทางพันธุกรรม; ลักษณะทางสัณฐานวิทยา; นาที่สูง.
Ano: 2013
URL: http://anchan.lib.ku.ac.th/agnet/handle/001/5599

Virulence and rep-PCR analysis of Pyricularia grisea isolates from two Brazilian upland rice cultivars
Provedor de dados: 102
Autores: Prabhu,Anne S.; Araújo,Leila G.; Silva,Gisele B.; Trindade,Maria G.
The phenotypic and genetic diversity of 77 isolates of Pyricularia grisea collected from two upland rice cultivars, Maravilha and Primavera, was studied. Isolates exhibiting compatible reaction to cv.Primavera were incompatible to cv.Maravilha and vice versa, with the exception of six isolates that were compatible to both cultivars. The virulence of isolates from cv. Maravilha on 32 test genotypes of rice was significantly higher (t = 9.09, p &lt; 0.0001) than the isolates from cv.Primavera. A phenogram constructed from virulence data showed two main groups, one constituted mainly of isolates from cv.Primavera (97.6%) and the other of isolates from cv.Maravilha (91.17%). Rep-PCR analysis of isolates using two primers designed from sequences of Pot2 showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Oryza sativa; Rice blast; Magnaporthe grisea; Pathotypes; Genetic diversity.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582007000100002

Telomere and microsatellite primers reveal diversity among Sclerotinia sclerotiorum isolates from Brazil
Provedor de dados: 102
Autores: MEINHARDT,LYNDEL W.; WULFF,NELSON A.; BELLATO,CLÁUDIA M.; TSAI,SIU M.
Sclerotinia sclerotiorum, the causal agent of white mold, is a problem of winter bean (Phaseolus vulgaris) production in Brazil under center-pivot irrigation. Isolates of S. sclerotiorum were obtained from a center-pivot-irrigated field near Guaíra-SP, Brazil. Mycelial compatibility group (MCG) studies revealed the presence of only two MCG. PCR/RFLP analysis of the ITS1-5.8S-ITS2 ribosomal subunit regions of these field isolates of S. sclerotiorum failed to show any genetic differences between these two MCGs. DNA amplification with a chromosomal telomere sequence-based primer and one microsatellite primer revealed genetic polymorphisms among isolates within the same MCG. Isolates taken from beans and two other crops from another region of Brazil showed the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genetic diversity; Mycelial compatibility group; Phaseolus vulgaris.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582002000200015

Caracterización con RAMs de la colección de durazno (Prunus persica L. Batsch) existente en la Universidad Pedagógica y Tecnológica de Colombia
Provedor de dados: 109
Autores: Morillo Coronado,Ana Cruz; Morillo Coronado,Yacenia; Pinzón Sandoval,Elberth Hernando.
Utilizando ocho cebadores Microsatélites Amplificados al Azar (RAMs) fue evaluada la diversidad genética de 31 materiales de Prunus de la colección de caducifolios existente en la Universidad Pedagógica y Tecnológica de Colombia. Se generaron un total de 121 amplicones con pesos moleculares entre 260 y 1000 Kb. Con un coeficiente de similitud de 0.75, se formaron tres grupos, de acuerdo principalmente con las características del fruto, encontrando en el grupo 2 las variedades de importancia económica. El número de loci polimórficos varió entre 5 y 16 para los cebadores GT y CCA, respectivamente. El valor promedio de heterocigosidad fue de 0.22, más bajo que los encontrados en otros estudios de diversidad genética en el género Prunus; por tanto, se deben...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Marcadores genéticos; Prunus; Diversidad genética; Microsatélites RAMs; Caducifolios; Genetic markers; Prunus; Genetic diversity; Microsatellites RAMs; Deciduous fruit trees.
Ano: 2014
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122014000400009

Molecular characterization of Iranian dromedaries using microsatellite markers
Provedor de dados: 109
Autores: Mohammadabadi.,Mohammadreza; Meymandi,Mehrdad Ghasemi; Montazen,Mahdieh; Afanasenko,Volodymyr; Kalashnyk,Oleksandr.
Abstract Considering the importance of maintaining the genetic diversity in native animals, this study conducted to analyse genetic diversity in dromedary populations in the north of Kerman province, Iran, using eight autosomal microsatellite markers. Eighty-one blood samples were collected from five different populations and DNA was extracted. The highest and the lowest alíele number and effective alíeles were shown in YWLL08 (21 and 4) and VOLP32 (14.97 and 3.11), respectively. The expected heterozygosity varied from 0.778 in Sahra-e Jahad population to 0.847 in Nogh population. The test for Hardy-Weinberg equilibrium showed significant deviations in most loci. The mean multilocus Fst value (0.057) suggested that differentiation is modérate between...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Iranian dromedary populations; Microsatellites; Population genetic structure.
Ano: 2020
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-28122020000400321

Molecular characterization of progenies of bacurizeiro ( Platonia insignis ) from Marajó Island, northeastern Amazon
Provedor de dados: 36
Autores: PONTES,Lígia Cristine Gonçalves; MOURA,Elisa Ferreira; MOURA,Mônika Fecury; RODRIGUES,Simone de Miranda; OLIVEIRA,Maria do Socorro Padilha de; CARVALHO,José Edmar Urano de; THERRIER,Josette.
ABSTRACT The bacurizeiro (Platonia insignis Mart.) is a tree native to the Amazon whose fruit is much used in the gastronomy in the North and Northeast regions of Brazil. Due to its great economic potential for these regions, the species is being conserved in germplasm banks to support genetic breeding programs. The aim of this work was the molecular characterization of P. insignis accessions belonging to the germplasm bank of the Embrapa Eastern Amazon research unit using ISSR (Inter Simple Sequence Repeat) markers. Seventy-eight accessions of P. insignis belonging to 16 progenies were sampled in two different localities on Marajó Island, state of Pará, Brazil. Among the 16 progenies, seven were collected in Soure and nine in Salvaterra. The 78 accessions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Germplasm bank; Clusiaceae.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672017000400293

Genetic diversity in rosewood saplings (Aniba rosaeodora ducke, Lauraceae): an ecological approach
Provedor de dados: 36
Autores: Santos,Ronaldo Pereira; Spironello,Wilson Roberto; Sampaio,Paulo de Tarso Barbosa.
This article takes an ecological approach to the genetic diversity of Rosewood (Aniba rosaeodora Ducke) in a central Amazonian terra firme forest north of Manaus. Planted Rosewood setting, under partial shaded canopy, were assessed in terms of fruiting production, frugivory, and seed dispersal. Using RAPD molecular analysis procedures, the influence of the spatial distribution of adult trees on the genetic diversity (polymorphism) of saplings was assessed with genetic samples from 34 reproductive trees and 60 saplings. The density and distribution patterns the reproductive trees did not modify the sapling"s diversity (1.86%, AMOVA). Two types of adult tree dispersion were identified; i) clumped and ii) more widely dispersed. Polymorphism (77.5%) and gene...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Amazonian tree; RAPD; Seed dispersal; Gene flow; Genetic diversity.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672008000400014

Pollen and seed dispersal of Brazil nut trees in the southwestern Brazilian Amazon
Provedor de dados: 36
Autores: MARTINS,Karina; SANTOS,Raifanny da Silva Oliveira dos; CAMPOS,Tatiana de; WADT,Lúcia Helena de Oliveira.
ABSTRACT Pollen and seed dispersal patterns greatly influence the spatial distribution of plant genetic diversity. Microsatellite-based parentage analysis provides accurate estimates of contemporary gene dispersal. Although most tropical trees have been shown to exhibit widespread pollen dispersal, few studies have estimated contemporary gene dispersal after seedling establishment. Bertholletia excelsa (Lecythidaceae) is pollinated by large-bodied bees, while previous seed-tracking experiments suggest their seeds are mainly dispersed across very short distances by scatter-hoarding rodents, who primarily act as seed predators. Here we used parentage analysis to provide contemporary estimates of pollen and seed dispersal in B. excelsa recruits. We examined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bertholletia excelsa; Gene flow; Genetic diversity; Pollen flow; Seed flow.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0044-59672018000300217

SYSTEMATIC CONSIDERATIONS ON TWO SPECIES OF NECTARIVOROUS BATS (Anoura caudifer AND A. geoffroyi) BASED ON BARCODING SEQUENCES
Provedor de dados: 37
Autores: VARGAS-ARBOLEDA,Andrés; CUADRADO-RÍOS,Sebastian; MANTILLA-MELUK,Hugo.
ABSTRACT As a product of recent revisions of the nectivore bat genus Anoura (Glossophaginae: Anourina), new species have been described, and several taxa have been elevated to the specific level in the so-called Anoura caudifer and A. geoffroyi species complexes in the last four decades. The genus has a wide distribution across regions of high ecological complexity, including geographic features, such as the Andes and the Isthmus of Panamá, previously identified as natural barriers that restrict gene flow in other phyllostomid bat genera. Up to date, no analyses have been conducted to investigate the contribution of biogeographic factors on the genetic divergence of the genus. In this work, we analyze barcoding DNA sequences (cytochrome oxidase subunit I,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ecuadorian pacific; Gene flow; Genetic diversity; Tailless bat.
Ano: 2020
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2020000200194

Using AFLP-RGA markers to assess genetic diversity among pigeon pea (Cajanus cajan) genotypes in relation to major diseases
Provedor de dados: 39
Autores: Pati,Prakash G; Datta,Subhojit; Agbagwa,Ikechukwu O; Singh,Indra Prakash; Soren,Khela Ram; Das,Alok; Choudhary,Arbind Kumar; Chaturvedi,Sushil Kumar.
Resistance gene analog (RGA)-anchored amplified fragment length polymorphism (AFLP-RGA) marker system was used in order to evaluate genetic relationships among 22 pigeon pea genotypes with varied responses to Fusarium wilt and sterility mosaic disease. Five AFLP-RGA primer combinations (E-CAG/wlrk-S, M-GTG/wlrk-S, M-GTG/wlrk-AS, E-CAT/S1-INV and E-CAG/wlrk-AS) produced 173 scorable fragments, of which 157 (90.7%) were polymorphic, with an average of 31.4 fragments per primer combination. The polymorphism rates obtained with the five primers were 83.3%, 92.0%, 92.3%, 93.0% and 93.1%, respectively. Mean polymorphic information content (PIC) values ranged from 0.24 (with E-CAT/S1-INV) to 0.30 (with E-CAG/wlrk-AS), whereas resolving power (RP) values varied...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: AFLP-RGA; Cajanus cajan; Genetic diversity; Molecular markers; Polymorphism.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062014000200007

Cross-amplification and characterization of microsatellite markers in species of Manihot Mill. (Euphorbiaceae) endemic to the Brazilian Cerrado
Provedor de dados: 39
Autores: Miranda,Kássia Marques Corrêa; Guimarães,Rejane Araújo; Silva,Marcos José da; Oliveira,Patrícia Rasteiro Ordiale; Ribeiro,Thania Gonçalves; Mendes,Thainara Policarpo; Telles,Mariana Pires de Campos; Soares,Thannya Nascimento.
ABSTRACT The genus Manihot Mill. contains about 120 species of which about 104 occur in Brazil. We tested the cross-amplification of ten microsatellite markers developed for Manihot esculenta in 15 species of Manihot endemic to the Brazilian Cerrado. We also evaluated the genetic diversity of Manihot irwinii, M. orbicularis, and M. purpureocostata. Ten pairs of primers were amplified among 14 species of Manihot. The percentage of polymorphic loci per species varied from 70 to 100 %. Nine markers showed amplification and polymorphism when evaluated on polyacrylamide gel. The markers were combined to form three sets for multiplex genotyping for genetic diversity analysis, and showed 51, 75, and 75 alleles in M. irwinii, M. orbicularis, and M....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Polymorphism; SSR; Transferability wild cassava.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400772

Morphological and genetic perspectives of hybridization in two contact zones of closely related species of Petunia (Solanaceae) in southern Brazil
Provedor de dados: 39
Autores: Teixeira,Marcelo C.; Turchetto,Caroline; Hartke,Sara; Schnitzler,Carolina K.; Freitas,Loreta B.
ABSTRACT Interspecific hybridization has several consequences for parental species, from blurring species limits to the emergence of new taxa. Petunia axillaris and P. exserta occur in sympatry in southernmost Brazil and naturally hybridize despite their different pollination syndromes and habitat requirements. We employed genetic and morphological analyses to characterize two contact zones between the species with the aim of determining the effect of interspecific hybridization. Microsatellite loci and a morphometric evaluation of the corolla shape were used to classify individuals based on their origin as pure parental or hybrids. Corolla color was used to classify individuals a priori (white, red or intermediate, for P. axillaris, P. exserta or hybrid,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Hybridization; Introgression; Morphometric analysis; Petunia.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400734

Genetic diversity in Calibrachoa pygmaea (Solanaceae): A hawkmoth-pollinated nightshade from the Pampas
Provedor de dados: 39
Autores: Mäder,Geraldo; Backes,Alice; Reck-Kortmann,Maikel; Freitas,Loreta B.
ABSTRACT Calibrachoa pygmaea is a unique species of Calibrachoa, especially concerning its flower morphology and the environment where it occurs. The species is self-incompatible and is narrowly distributed in wet and flooded fields of the Pampas region. We characterize the genetic diversity of the species based on traditional plastid markers and newly developed nuclear microsatellites to identify drivers that guide its evolution. Our results identified markers that are informative and useful for studying the population structure of C. pygmaea, as well as that of other species of Calibrachoa. Both marker sets were congruent in developing conclusions regarding the evolutionary scenario of C. pygmaea, and revealed that the genetic variability and population...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cross-amplification; Gene flow; Genetic diversity; Plastid haplotypes; Population genetics; SSR markers; Wild petunias.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062019000400664

Genetic diversity in populations of Maytenus dasyclada (Celastraceae) in forest reserves and unprotected Araucaria forest remnants
Provedor de dados: 39
Autores: Reichmann,Manuel Castilhos; Zanella,Camila Ângela; Valério Júnior,Cláudio; Borges,Ana Claudia Piovezan; Sausen,Tanise Luisa; Paroul,Natalia; Mielniczki-Pereira,Albanin Aparecida; Teixeira,Amito José; Budke,Jean Carlos; Mossi,Altemir José; Cansian,Rogério Luis.
ABSTRACT Understanding the genetic structure and diversity of plants is fundamental to their conservation and permits their sustainable use by local communities. The genus Maytenus (Celastraceae) is composed of plants possessing pharmacological and antioxidant properties. However, the genetic and economic properties of the species M. dasyclada, a typical species of Araucaria forests in Brazil and Uruguay, have been little studied. In this work, the genetic structure and diversity of natural populations of M. dasyclada located in unprotected and preserved forest remnants were investigated using RAPD and isozymes markers. The results demonstrated that in areas of preservation, populations of M. dasyclada possess a relatively high degree of polymorphism and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Forest reserves; Genetic diversity; Maytenus dasyclada; Molecular markers.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062017000100093

Genetic differentiation of the neotropical tree species Protium spruceanum (Benth.) Engler (Burseraceae) between fragments and vegetation corridors in Brazilian Atlantic forest
Provedor de dados: 39
Autores: Vieira,Fábio de Almeida; Carvalho,Dulcinéia de.
We studied patterns of genetic differentiation in a connected landscape with an interesting history of human habitat conversion that began two centuries ago, during the Brazilian colonization period. In the fragments of Brazilian Atlantic seasonal forest and corridors of secondary forest, Protium spruceanum is an abundant native, mass-flowering/insect-pollinated and bird-dispersed tree. Genetic diversity was analyzed from 230 individuals in five fragments (1 to 11.8 ha) and four corridors (460 to 1000 m length) using allozyme loci. We did not find evidence of inbreeding within fragments or corridors, but the proportion of heterozygotes (&lt;IMG SRC="/img/revistas/abb/v23n4/a28simb1.gif" WIDTH=287 HEIGHT=96&gt;) were significantly higher in fragments than...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Allozymes; F-statistics; Genetic diversity; Habitat fragmentation.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062009000400028

Genetic structure of Dicksonia sellowiana Hook. (Dicksoniaceae) reveals clinal distribution along the latitudinal gradient of the Atlantic Forest
Provedor de dados: 39
Autores: Fagundes,Bruna Saviatto; Poersch,Maria Augusta; Santos,Jaqueline dos; Gaglioti,André Luiz; Labiak,Paulo Henrique; Muschner,Valeria Cunha.
ABSTRACT Dicksonia sellowiana is the only species of the genus occurring in Brazil. Its distribution is restricted to humid areas of the Atlantic Forest biome. The distribution pattern of biodiversity in this biome is known to have been influenced by historical and environmental factors, although the pattern for ferns remains unknown. This study is first to describe the genetic structure of D. sellowiana along the latitudinal gradient of the Atlantic Forest biome. We use microsatellite markers to estimate genetic diversity and structure for 267 individuals representing 14 populations of D. sellowiana from the Atlantic Forest. The results (Ho, He, Fst, Fis, distance genetic) support the hypothesis of a pattern of biodiversity discontinuity. We found greater...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Neotropics; Gene flow; Genetic diversity; Microsatellites; Tree fern.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062020000400712

Allelic diversity in populations of Solanum lycocarpum A. St.-Hil (Solanaceae) in a protected area and a disturbed environment
Provedor de dados: 39
Autores: Moura,Tânia Maria de; Sebbenn,Alexandre Magno; Martins,Karina; Moreno,Maria Andreia; Oliveira,Giancarlo Conde Xavier; Chaves,Lázaro José; Kageyama,Paulo Yoshio.
This study aimed to compare the genetic diversity of populations of Solanum lycocarpum A.St.-Hil between natural and human disturbed environments, with the assumption that protected areas have greater genetic diversity than disturbed areas. For this study, two populations were sampled in Goiás State, Brazil. One was located in a conservation unit, Serra de Caldas Novas State Park, in the Caldas Novas municipality. The other was located in a pasture area in the municipality of Morrinhos. The two populations are 41 km apart. We sampled 60 individuals from each population, which were genotyped with five microsatellite loci (SSR). The highest number of alleles was recorded in the population of the conservation unit, where we found 11 exclusive and five rare...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Gene flow; Conservation genetics; Genetic diversity.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062011000400023

Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry
Provedor de dados: 1
Autores: Oñate, Felipe Alberto; Hasbún, Rodrigo; Mora, Freddy; Figueroa, Carlos Rodrigo.
 The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; Chilean strawberry; Marker transferability.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34966

Linkage disequilibrium and population structure in Fragaria chiloensis revealed by SSR markers transferred from commercial strawberry
Provedor de dados: 1
Autores: Oñate, Felipe Alberto; Hasbún, Rodrigo; Mora, Freddy; Figueroa, Carlos Rodrigo.
 The Chilean strawberry [Fragaria chiloensis (L.) Mill.] is the maternal progenitor of the commercial strawberry (Fragaria ´ ananassa Duch.), which is characterized by fruits with high organoleptic quality and is well-suited to areas where drought and salinity represent a constraint on crop growth and productivity. We examined the patterns of linkage disequilibrium, genetic diversity and population structure among 54 accessions of F. chiloensis to understand the genetic basis of this species. We used a core microsatellite marker set (n = 95) from a consensus linkage map of strawberry. A transferability rate of 82.1% (78/95) was found, and 38 markers were selected for this study. The SSR primers produced a total of 259 alleles, which varied between 112 and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Microsatellites; Chilean strawberry; Marker transferability.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/34966

Hierarchical genetic clusters for phenotypic analysis
Provedor de dados: 1
Autores: Matta, Luiza Barbosa da; Tomé, Lívia Gracielle Oliveira; Salgado, Caio Césio; Cruz, Cosme Damião; Silva, Letícia de Faria.
Methods to obtain phenotypic information were evaluated to help breeders choosing the best methodology for analysis of genetic diversity in backcross populations. Phenotypes were simulated for 13 characteristics generated in 10 populations with 100 individuals each. Genotypic information was generated from 100 loci of which 20 were taken at random to determine the characteristics expressing two alleles. Dissimilarity measures were calculated, and genetic diversity was analyzed through hierarchical clustering and graphic projection of the distances. A backcross was performed from the two most divergent populations. A set of characteristics with variable heritability was taken into account. The environmental effect was simulated assuming . For hierarchical...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genética; Diversidade Genética hierarchical clustering; Genetic diversity; Backcrossing.
Ano: 2015
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/19746

Population structure of Monosporascus cannonballus isolated from melons produced in Northeastern Brazil based on mycelial compatibility groups - doi: 10.4025/actasciagron.v35i2.15182
Provedor de dados: 1
Autores: Bezerra, Cíntia Souza; Universidade Federal Rural de Pernambuco; Correia, Kamila Câmara; Universidade Federal Rural de Pernambuco; Câmara, Marcos Paz Saraiva; Universidade Federal Rural de Pernambuco; Sales Junior, Rui; Universidade Federal Rural do Semi-Árido; Armengol, Josep; Instituto Agroforestal Mediterráneo; Michereff, Sami Jorge; Universidade Federal Rural de Pernambuco.
The population structure of Monosporascus cannonballus, which causes vine decline in melons, was assessed based on the determination of mycelial compatibility groups (MCGs) in a collection of 58 isolates obtained from seven melon fields in three municipalities of Northeastern  Brazil. For comparison, an additional 11 isolates of M. cannonballus from Spain were included in the analysis. MCGs were determined through comparisons of paired isolates growing on PDA culture media in the dark at 30ºC in various combinations. The Brazilian isolates were assigned into four MCGs: MCG-1 (n = 35 isolates), MCG-2 (n = 20), MCG-3 (n = 2), and MGC-4 (n = 1). MCG-1 and MCG-2 included isolates from all surveyed areas. The Spanish isolates were assigned into six different...
Tipo: Pesquisa científica
Palavras-chave: 5.01.02.01-0 Cucumis melo; Melon; Vine decline; Soilborne pathogen; Genetic diversity Fitopatologia Cucumis melo; Melon; Vine decline; Soilborne pathogen; Genetic diversity.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15182

Genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors
Provedor de dados: 1
Autores: Andrade, Elisângela Knoblauch Viega de; Andrade júnior, Valter Carvalho de; Laia, Marcelo Luiz de; Fernandes, José Sebastião Cunha; Oliveira, Altino Junior Mendes; Azevedo, Alcinei Mistico.
 This study aimed to evaluate the genetic dissimilarity among sweet potato genotypes using morphological and molecular descriptors. The experiment was conducted in the Olericulture Sector at Federal University of Jequitinhonha and Mucuri Valleys (UFVJM) and evaluated 60 sweet potato genotypes. For morphological characterization, 24 descriptors were used. For molecular characterization, 11 microsatellite primers specific for sweet potatoes were used, obtaining 210 polymorphic bands. Morphological and molecular diversity was obtained by dissimilarity matrices based on the coefficient of simple matching and the Jaccard index for morphological and molecular data, respectively. From these matrices, dendrograms were built. There is a large amount of genetic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Fitotecnia Ipomoea batatas (L.) Lam.; Morphological markers; Microsatellites; Genetic diversity; Germplasm bank. Melhoramento genético de plantas.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/32847

Development and characterization of microsatellite loci for Campomanesia xanthocarpa (Myrtaceae) and cross amplification in related species
Provedor de dados: 3
Autores: Góes, Bruna Delgado; Ruas, Eduardo Augusto; Benício, Lucas Milanez; Cassiano, Daniele; Souza, Felipe Pinheiro de; Ruas, Paulo Maurício.
Campomanesia xanthocarpa is a native tree, of common occurrence in almost all Brazilian Forest formations, which has its fruits and timber with high commercial value. Using an enriched genomic library we isolated and characterized microsatellite loci for C. xanthocarpa (Myrtaceae), in order to estimate genetic diversity parameters for this and related species. Twenty-eight microsatellite loci were identified and ten of them successfully amplified and showed polymorphism in a sample of 96 individuals, from four natural populations. The number of alleles per locus ranged from two to eight, and the observed and expected heterozygosities varied from 0.042 to 1.000 and from 0.294 to 0.855, respectively. These markers were tested and validated in two related...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ciências Biológicas gabiroba; Genetic diversity; Molecular marker; Native tree; Simple sequence repeat (SSR) Desenvolvimento de Marcadores Simple Sequence Repeats.
Ano: 2019
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/43454

Genetic variability in Oligosarcus paranensis (Teleostei: Characiformes) from the São Francisco river, Ivaí river basin – Paraná State, Brazil - doi: 10.4025/actascibiolsci.v35i3.14179
Provedor de dados: 3
Autores: Santos, Michele Rocha dos; Universidade Estadual de Maringá; Renesto, Erasmo; Universidade Estadual de Maringá.
The genetic variability of Oligosarcus paranensis was estimated from a population collected in São Francisco river, Prudentópolis county in Paraná State (Brazil) using the electrophoresis in starch gel technique. Eleven enzymatic systems were analyzed: Aspartate aminotransaminase (AAT; E. C. 2.6.1), Alcohol dehydrogenase (ADH; E. C. 1.1.1.1), Esterase (EST; E. C. 3.1.1.1), Glucose-6-phosphate isomerase (GPI; E. C. 5.3.1.9), Glycerol-3-Phosphate dehydrogenase (G3PDH; E. C. 1.1.1), Isocitrate dehydrogenase (IDH; E. C. 1.1.1.42), L-lactate dehydrogenase (LDH; E. C. 1.1.1.27), Malate dehydrogenase (MDH; E. C. 1.1.1.37 ), Malate dehydrogenase NADP (ME; E. C. 1.1.1.40), Phosphoglucomutase (PGM; E. C. 5.4.2.2) and Sorbitol dehydrogenase (SORB; E.C. 1.1.1.14)....
Palavras-chave: Allozyme; Genetic diversity; Heterozygosity; Polymorphism; Pisces.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/14179

Genetic similarities among four species of the Plectranthus (L’Hér.) genus - DOI: 10.4025/actascibiolsci.v32i1.880
Provedor de dados: 3
Autores: Bandeira, Juliana Magalhães; UFPel; Bianchi, Valmor João; UFPel; Rubin, Sílvia; UFPel; Peters, José Antonio; UFPel; Braga, Eugenia Jacira Bolacel; UFPel.
The Plectranthus genus comprises several species generally referred to as boldo, which are highly used in popular medicine due to their anti-dyspeptic, analgesic and digestion-stimulating properties. The amount of natural active principles can vary greatly in the related genotypes, which may lead to the inappropriate use of these plants. This work aimed to analyze the interspecific diversity among four species of the Plectranthus genus (P. grandis, P. barbatus, P. neochilus and P. amboinicus) and the intraspecific diversity of P. barbatus collected from different places in southern Brazil, by means of the RAPD technique. A higher genetic similarity was observed between the P. neochilus and P. amboinicus species (80%), followed by P. grandis and P. barbatus...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RAPD; Boldo; Genetic diversity; Medicinal plants Botânica; Genética Vegetal; Genética Molecular e de Microrganismos; Ciências Biológicas.
Ano: 2009
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/880

Genetic variability and diversity of mutant rice revealed by quantitative traits and molecular markers
Provedor de dados: 115
Autores: Oladosu,Yusuff; Rafii,Mohd Y.; Abdullah,Norhani; Malek,Mohammad Abdul; Rahim,Harun A.; Hussin,Ghazali; Ismail,Mohd Razi; Latif,Mohammad Abdul; Kareem,Isiaka.
Genetic variability with mutagenic agents has been employed in plant breeding due to its significant contribution to the improvement of the existing rice genotypes by using genetically diverse parents. This study evaluated the genetic variability and diversity of mutant rice using quantitative traits and inter-simple sequence repeats (ISSR) molecular markers. A field experiment was carried out on M5 generation of 31 developed mutant lines and their parent (control). Morphological traits and 25 ISSR molecular markers were used as tools for determining cultivar identities and genetic diversity. The selected primers generated 443 clear polymorphic bands with an average number of 18 per primer. The bands were 85.10 % polymorphic. Un-weighted Pair Group of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant breeding; Genetic diversity; Molecular marker; Mutagenicity; Polymerase chain reaction (PCR).
Ano: 2015
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952015000300002

Genetic variability and selection in natural populations of vineyard peach (Prunus persica ssp. vulgaris Mill.) in the Krusevac region (Central Serbia)
Provedor de dados: 115
Autores: Milosevic,Tomo; Milosevic,Nebojsa.
In situ selection of vineyard peach (Prunus pérsica spp. vulgaris Mill.) genotypes was conducted in 2006 and 2007 in the region of Kfusevac (Central Serbia). Identification, observation and recording of the phenological and pomological properties of the highest-quality genotypes were carried out. Twenty-eight genotypes of the highest quality were selected out of 2093 genotypes, based on biological and pomological properties, and compared to those of cv. Redhaven (control). The onset of flowering varied (April 7-21), being early in two genotypes and late in one genotype, as compared to Redhaven, while 25 genotypes showed an intermediate flowering date, as Redhaven did. Campanula flowers were found in six genotypes, as in Redhaven, and the rosaceous type in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Breeding; Flowering; Fruit and stone; Genetic diversity; Genotype.
Ano: 2010
URL: http://www.scielo.org.mx/scielo.php?script=sci_arttext&pid=S1405-31952010000300005

Unraveling the genetic complexity of a cultivated breeding population of “yerba mate” (Ilex paraguariensis St. Hil.)
Provedor de dados: 42
Autores: PAIVA,DANIELA IVANA; CASCALES,JIMENA; ROSETTI,MARÍA EVA NATALIA; SCHERER,RAFAEL ALEJANDRO; GAUCHAT,MARÍA ELENA; GOTTLIEB,ALEXANDRA MARINA.
Abstract  We report the first comprehensive multilocus molecular characterization of cultivated Ilex paraguariensis plants belonging to a breeding program. Using nuclear and homologous chloroplastidic molecular markers, we have genotyped 158 plants from four plantation sites. Analyses of the nuclear data (187 variable dominant loci) allowed detecting high diversity (0.569), the occurrence of four distinct genetic clusters, and a low but significant differentiation among sites. Additionally, 20 chloroplastidic alleles were identified applying five microsatellite polymorphic markers, and a high chloroplastidic diversity was recognized (0.505); two haplogroups were distinguished amongst the 63 haplotypes detected. Our results from both nuclear and plastidic...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chloroplastidic microsatellites; Genetic diversity; Genotyping; ISSR markers.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652020000100717

Genetic diversity and nonparametric statistics to identify possible ISSR marker association with fiber quality of pineapple
Provedor de dados: 42
Autores: SILVA,JULIANNA M.; LIMA,PAULO R.L.; SOUZA,FERNANDA V.D.; LEDO,CARLOS A.S.; SOUZA,EVERTON H.; PESTANA,KATIA N.; FERREIRA,CLÁUDIA F.
Abstract: Due to the increasing search for renewable resources, plant fibers have become an alternative when creating new products. Studies demonstrate the potential use of pineapple fibers in composites. The objective of this work was to evaluate the genetic diversity and verify any association between ISSR (Inter Simple Sequence Repeats) bands and quality of pineapple fibers for use in cements in the civil construction. The study analyzed the genetic variability of 11 pineapple genotypes, as well as the possible association of 131 bands from 16 ISSR markers with fiber quality characteristics. Eleven bands were selected based on their high correlations (0.64578* to 0.72457*) with three fiber quality variables. Of these, two bands were purified, sequenced,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus (L.) Merr.; Civil construction; Genetic diversity; DNA markers; Nonparametric statistics.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652019000500862

Genetic structure from the oldest Jatropha germplasm bank of Brazil and contribution for the genetic improvement
Provedor de dados: 42
Autores: SANTOS,DALILHIA N. DOS; FERREIRA,JULIANO L.; SETOTAW,TESFAHUN A.; CANÇADO,GERALDO M.A.; PASQUAL,MOACIR; LONDE,LUCIANA C.N.; SATURNINO,HELOISA M.; VENDRAME,WAGNER A.
ABSTRACT Jatropha is a potential oilseed crop, which requires mitigating factors such as the low genetic variability of the species. The solution runs through the research of Brazilian germplasm. Attention should be given to the germplasm of jatropha the north of Minas Gerais, because this is the oldest national collection and because this region may be a regions of jatropha diversity due to selection pressure arising from environmental adversities. Therefore, the objective of this study was to investigate the genetic diversity of 48 accessions of collection from Empresa de Pesquisa Agropecuária de Minas Gerais (EPAMIG), using SSR and ISSR markers. The results showed low genetic diversity, but some individuals stood out as J. mollissima (48), J. podagrica...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Jatropha curcas; Molecular markers; Oilseed crop.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652016000602363

Genetic and chemical diversity in seeds of cactus mandacaru (Cereus sp.) from two edaphoclimatic regions contrasting
Provedor de dados: 42
Autores: Bevilaqua,Maycon R.R.; Santana Filho,Arquimedes P.; Mangolin,Claudete A.; Oliveira,Arildo J.B.; Machado,Maria De Fátima P.S.
The purpose of this study was to evaluate the chemical, physiological and genetic differences in seeds of cactus of the Cereus genus (mandacaru) cultivated in the Northeast (Picos, State of Piauí) and Southern (Maringá, State of Paraná) regions of Brazil. Over a period of eight days, temperatures of 25°C and 30°C were equally efficient for the germination of all the seeds. Oleic acid (C18:1) was the most common fatty acid found in the seeds collected in the Southern (41%) and Northeast (45.5%) regions. The analysis of lipases indicated that seeds from Maringá have high mean observed and expected heterozygosities and that seeds from Picos have a higher number of alleles per loci. Therefore, the seeds of mandacaru from the semiarid region of Northeast as...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cactus; Cereus peruvianus; Cereus jamacaru; Seed germination; Fatty acids; Lipases; Genetic diversity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652015000200765

Is the Murciano-Granadina a single goat breed? A molecular genetics approach
Provedor de dados: 45
Autores: Martínez,A.M.; Vega-Pla,J.L.; León,J.M.; Camacho,M.E.; Delgado,J.V.; Ribeiro,M.N.
The population structure of the Murciano-Granadina breed was determined using 25 microsatellites from 266 goats of seven populations. The results of the genetic differentiation analysis showed that it is possible to differentiate the Murciana and Granadina populations even though a low F ST value (0.0432) had been obtained. Individuals could be assigned to their populations with a success rate of more than 80%. Bayesian-based clustering analysis of allele frequencies and multivariate analysis revealed that Murciana and Granadina populations were grouped in different clusters since K=3. The results demonstrate that Murciana and Granadina are still two different genetic groups included into Murciano-Granadina denomination. There is the opportunity to the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dairy goat; Microsatellites; Genetic diversity; Conservation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352010000500023

Genetic characterization of pomegranate (Punica granatum L.) genotypes by AFLP markers
Provedor de dados: 48
Autores: Ercisli,Sezai; Kafkas,Ebru; Orhan,Emine; Kafkas,Salih; Dogan,Yildiz; Esitken,Ahmet.
The Coruh Valley, located in Northeastern Turkey, is one of the most important centers of diversity in pomegranate in Turkey. In this study, we attempted to characterize 19 promising pomegranate genotypes originating from the Coruh Valley in using fluorescent dye AFLP markers and capillary electrophoresis. Four AFLP primer combinations were used, generating a total of 297 fragments, 213 of which were polymorphic (73.0%). Resolving powers of the AFLP primers ranged from 0.700 to 1.018, with a total of 3.440, while polymorphism information contents ranged from 0.707 to 0.837 with an average of 0.764. UPGMA clustering of the genotypes showed two major groups. Most of the fruit characteristics of the genotypes within the same group were variable. Therefore,...
Tipo: Journal article
Palavras-chave: Genetic diversity; AFLP; Pomegranate.
Ano: 2011
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602011000400005

Genetic structure of the ornamental tetra fish species Piabucus melanostomus Holmberg, 1891 (CHARACIDAE, IGUANODECTINAE) in the Brazilian Pantanal wetlands inferred by mitochondrial DNA sequences
Provedor de dados: 49
Autores: Borba,Rafael Splendore de; Silva,Edson Lourenço da; Ponzetto,Josi Margarete; Pozzobon,Allan Pierre Bonetti; Centofante,Liano; Alves,Anderson Luis; Parise-Maltempi,Patrícia Pasquali.
The subfamily Iguanodectinae comprises a group of small Neotropical fishes composed by two genera and 11 nominal species widely distributed in the Atlantic drainages of South America. Piabucus is the only genus of Iguanodectinae found in the Paraguay River basin, especially in the Pantanal of Mato Grosso State, where it is represented by Piabucus melanostomus. Given the wide distribution and the low dispersion capacity of this species, due the ecological constraints, it is possible that many interesting genetic features could be found in different populations. In this way, the aim of his work was to perform the phylogeographic pattern of P. melanostomus populations using mitochondrial DNA sequences. A total of 13 individuals from three rivers belonging the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flood pulse; Genetic diversity; Gene flow; Haplotypes.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032013000100004

Transferability and characterization of microsatellite markers in five Bromeliaceae species belonging to the subfamilies Pitcairnioideae and Bromelioideae
Provedor de dados: 49
Autores: Miranda,Fabio Demolinari de; Gontijo,Andreia Barcelos Passos Lima; Santiliano,Fabiano Costa; Favoreto,Fernanda Campanharo; Soares,Taís Cristina Bastos.
Microsatellite markers previously developed for Pitcairnia albiflos Herb. and Pitcairnia geysksii L.B.Sm. were used in cross-amplification tests of five other Bromeliaceae species. Ten (76.9%) out of the 13 evaluated pair of primers had positive results for some of the species tested. The number of polymorphic alleles ranged between two and four in most species. Ho values ranged between zero, in Pitcairnia flammea Lindl. (PaA05), Aechmea ramosa Mart ex Schult &amp;amp; Schult and Billbergia horrida Regel (PaC05), and one in Billbergia euphemiae E. Morren (PaA05, PaA10, PaC05 and PaD07). This study showed that microsatellite markers developed for P. albiflos and P. geysksii effectively amplified the DNA samples of Pitcairnia flammea, Aechmea nudicaulis (L.)...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Cross-amplification; SSR; Genetic diversity; Bromeliads; Conservation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1676-06032012000300032

Genotypic Diversity of the Wheat leaf blotch pathogen ( Septoria tritici ) in Buenos Aires Province
Provedor de dados: 51
Autores: Cordo,Cristina Alicia; Linde,Celeste C.; Zhan,Jashiu; Mcdonald,Bruce.
The population structure and genotypic diversity of Septoria tritici Rob ex Desm. from two crop field populations in Buenos Aires Province were studied with DNA restriction fragment length polymorphism. Hierarchical samples were taken from different cultivars. A total of 120 single spore isolates was subject to molecular analysis to compare the genetic structure of Los Hornos and Balcarce populations separated by 500km. Eight RFLP loci and 1DNA fingerprinting were used. Among the total of the isolates, 24 RFLP alleles were detected from the first population and 22 from the second. Gene and genotype diversity were high in both populations. Different haplotypes for each region were identified. Identical haplotypes were clustered in the same location in the...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: DNA hybridization; Septoria tritici; Mycosphaerella graminicola; RFLP; Epidemiology; Genetic diversity; Genotypic diversity.
Ano: 2006
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722006000200010

Genetic diversity in a natural population of the halophytic legume Prosopis strombulifera revealed by AFLP fingerprinting
Provedor de dados: 51
Autores: Llanes,Analia; Bonercarrere,Victoria; Capdevielle,Fabian; Vidal,Sabina; Luna,Virginia.
Prosopis strombulifera (Lam.) Benth. is a spiny shrub with the maximum tolerance limits reported for halophytic plants. This species is frequently found in the salinized areas in south-western of Córdoba and San Luis provinces, Argentina. Little is known about the genetic diversity within this species in a native population. Genetic diversity in 60 plants of P. strombulifera in south-western San Luis was investigated using AFLP analysis. Polymorphism was found among the samples tested. Four combinations of primers led to the identification of an average of 250 polymorphic bands and the data were used for cluster analysis. P. strombulifera genotypes are clearly separated in subclusters and reflect the diversity within the collection area. This study is a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Prosopis strombulifera; AFLP; Genetic diversity; Polymorphism.
Ano: 2011
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-23722011000200010

Genetic distances in soybean based on RAPD markers
Provedor de dados: 53
Autores: CORRÊA,RONAN XAVIER; ABDELNOOR,RICARDO VILELA; FALEIRO,FÁBIO GELAPE; CRUZ,COSME DAMIÃO; MOREIRA,MAURILIO ALVES; BARROS,EVERALDO GONÇALVES DE.
Four methods were applied to determine pairwise genetic distances among five soybean genotypes which are potential genitors for a mapping population. Additionally, individual plants from the most divergent pair of genotypes were evaluated by the RAPD technique to determine their degree of homozygosity. Genetic distances based on RAPD data were calculated by the modified Rogers' distance, and also by the following arithmetical complements of similarity: simple match, Nei and Li, and Gower. These genetic distances were similar, presenting a correlation coefficient ranging from 0.99 to 1.00. In all four methods lines UFV 91-717 and Ichigowase were the most divergent ones (4.53 to 21.43%). DNA samples from five plants from each of the two most divergent...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Genetic diversity; RAPD markers; Estimation methods.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87051999000100003

Genetic diversity and population densities of endophytic Bacillus spp. in yam plants
Provedor de dados: 53
Autores: Souza,Jorge Teodoro de; Santos,Ana Cláudia Oliveira Souza; Monteiro,Fernando Pereira; Batista,Mariah Rodrigues; Santos,Patrícia Oliveira dos; Carvalho,Larissa Ferreira; Corrêa,Elida Barbosa.
ABSTRACT This study was conducted as a first step in exploiting endophytic bacteria to improve yam production. Little information is available on population densities and genetic diversity of endophytic bacteria in yam cultivated in tropical regions. This study demonstrated that higher densities of total endophytic bacteria and Bacillus occur in the interior of roots and decrease as the sampled plant organ departs from soil. Bacillus represents approximately 1% of the total bacterial endophytic population. Random Amplified Polymorphic DNA (RAPD) analyses performed with 88 randomly-selected Bacillus isolates resulted in 28 groups, and sequence analyses of the 16S region of the ribosomal DNA of 24 isolates representing all major RAPD groups revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Dioscoriaceae; Genetic diversity; Population densities; Endophytes; Bacterial ecology.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000200203

A comparative genetic diversity assessment of industrial and household Brazilian cassava varieties using SSR markers
Provedor de dados: 53
Autores: Siqueira,Marcos Vinicius Bohrer Monteiro; Borges,Aline; Valle,Teresa Losada; Veasey,Elizabeth Ann.
This study was carried out in order to assess the genetic diversity of 20 cassava (Manihot esculenta Crantz) genotypes with high phenotypic performance using microsatellite markers. Two groups were considered for this study: eleven varieties for industrial uses and nine for household consumption. By using nine microsatellite primers, high polymorphism was identified in all the loci analyzed, with values reaching 100%. On average, 3.4 alleles per locus were found, with 0.371 the value estimated for the observed heterozygosity and 0.555 for gene diversity for the entire set of varieties. The genetic variability found in both varieties, cultivated on a large-scale in the South Center region of Brazil, is wide enough to allow the choice of divergent parental...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Manihot esculenta; Genetic diversity; Germplasm; Microsatellites.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052011000400003

Genetic Diversity among Flue-cured Tobacco Cultivars Based on RAPD and AFLP Markers
Provedor de dados: 52
Autores: Zhang,Han Yao; Liu,Xiao Zhen; He,Chuan Sheng; Yang,Yu Ming.
The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cluster analysis (UPGMA) based on RAPD data and AFLP data. The accessions were grouped into three major clusters and these shared common ancestry clustered together.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Flue-cured tobacco; RAPD; AFLP; Genetic diversity; DNA polymorphism.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000600003

Molecular characterization of arabica and Conilon coffee plants genotypes by SSR and ISSR markers
Provedor de dados: 52
Autores: Motta,Ludymila Brandão; Soares,Taís Cristina Bastos; Ferrão,Maria Amélia Gava; Caixeta,Eveline Teixeira; Lorenzoni,Rodrigo Monte; Souza Neto,José Dias de.
The molecular characterization of ten genotypes of the Coffea arabica plants and of seven genotypes of C. canephora having interesting features for coffee breeding programs was carried to select the parents for breeding. A total of 40 SSR and 29 ISSR primers were used. The primers generated a total of 331 (307 polymorphic and 24 monomorphic) bands. Analysis of genetic diversity presented dissimilarity intervals ranging from 0.22 to 0.44 between the Conilon genotypes, from 0.02 to 0.28 between the Arabica genotypes, and from 0.49 to 0.60 between the genotypes of the two species in the joint analysis. Four groups were formed: I = genotypes of C. arabica, II = four progenies of C. canephora, Conilon group, and one non defined C. canephora (Conilon or...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Coffea Arabica; Coffea canephora; Genetic diversity; Molecular markers.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000500728

Assessing the genetic structure of Oryza glumaepatula populations with isozyme markers
Provedor de dados: 52
Autores: Veasey,Elizabeth Ann; Cardin,Daruska; Silva,Rainério Meireles; Bressan,Eduardo de Andrade; Vencovsky,Roland.
To assess the genetic diversity and genetic structure parameters, nine populations of Oryza glumaepatula from the Amazon biome, four from the Pantanal biome, and one collected at Rio Xingu, Mato Grosso, totaling 14 populations and 333 individuals were studied with isozyme markers. Six loci were evaluated showing a moderate allozyme variability (A = 1.21, P = 20.7%, Ho = 0.005, He = 0.060). The populations from the Pantanal biome showed higher diversity levels than the Amazon biome. High genetic differentiation among the populations, expected for self-fertilizing species, was observed (F ST=0.763), with lower differentiation found among the Pantanal populations (F ST=0.501). The average apparent outcrossing rate was higher for the Pantanal populations (t a...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Isozymes; Oryza glumaepatula; Outcrossing rate; Populations.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500001

Use of RAPD molecular markers on differentiation of brazilian and chinese Ganoderma lucidum strains
Provedor de dados: 52
Autores: Rolim,Leonardo do Nascimento; Cavalcante,Maria Auxiliadora de Queiroz; Urben,Arailde Fontes; Buso,Glaucia Salles Cortopassi.
The aim of this work was to analyze the Brazilian and Chinese strains of Ganoderma lucidum with molecular RAPD markers. A similarity matrix was elaborated and the RAPD profiles of G. lucidum strains were also compared to two other Ganoderma spp: G. applanatum and G. lipsiense in order to produce genetic similarity among the species. Based on the primers used, it was possible to determine that the Brazilian strains and Chinese strain CC-22 are alike. The method and the primers selection showed to be appropriate for the genetic identification of G. lucidum strains, enabling them to be improved and used in research, as well as in the world market.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ganoderma lucidum; Genetic diversity; RAPD.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000200008

Efficiency of RAPD and ISSR markers in assessing genetic variation in Arthrocnemum macrostachyum (Chenopodiaceae)
Provedor de dados: 52
Autores: Saleh,Basel.
The goal of this study was to investigate the molecular characterization of A. macrostachyum genotypes grown in the Western coast of Syria using RAPD and ISSR techniques. PCR amplifications with 20 RAPD primers gave an average of 9.25 selected markers/ primer, with a maximum of 17 (OPG05) and a minimum of four (OPA02). Percentage of polymorphic bands ranged from 40 to 100% according to RAPD primers tested. Among the 185 selected bands, 160 (84.96%) were polymorphic. The amplification with seven ISSR primers generated 88 bands, and 80 (90.91%) were polymorphic. (CA)8RG and (AG)8GTG ISSR primers tested in this study yielded highly informative patterns. RAPD and ISSR fragment sizes ranged from 0.2-3 kb. Based on this study, the use of RAPD and ISSR...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A. macrostachyum; Genetic diversity; Genotype; ISSR; Polymorphism; RAPD.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500002

Temporal genotypic diversity of Schizaphis graminum (Rondani 1852) (Hemiptera: Aphididae) in a black oats (Avena strigosa) field
Provedor de dados: 52
Autores: Lopes-da-Silva,Marcelo; Vieira,Luiz Gonzaga Esteves.
The aim of this work was to analyze the clonal diversity variation in Schizaphis graminum during a complete phenological cycle of black oats (Avena strigosa). RAPD markers were used for detection of DNA polymorphisms of each clonal lineage, derived from a single clone collected weekly during a period of four months, in a crop field of black oats, Londrina, Paraná, Brazil. The monthly genotypic diversity was estimated by Shannon Information Index (H). Only four genotypes were distinguished from 122 specimens, with one of them overly predominant in all sampling dates (&gt;60%). Another genotype, apparently a later colonizer, rapidly reached greater frequency than other genotypes on the second and third month. The results of this work suggested that temporal...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; RAPD; Temporal scale.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400021

Genetic diversity in passion fruit (Passiflora spp.) evaluated by RAPD markers
Provedor de dados: 52
Autores: Crochemore,Maria Lúcia; Molinari,Hugo Bruno Correa; Vieira,Luiz Gonzaga Esteves.
The objective of this study was to characterize the genetic diversity within a Passiflora collection by PCR-RAPD markers. Genetic analysis was performed in 70 accessions, representing 11 species of the genus Passiflora. The use of only five primers produced 136 reproducible polymorphic bands. The hierarchical classification showed high levels of dissimilarities between and within the species studied. A clear separation was obtained among species and accessions of P. edulis and P. edulis f. flavicarpa, which were forms of the high commercial value species. The fingerprints produced from the studied genotypes would allow the identification of improved varieties/populations in an easy, fast and inexpensive manner.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Passiflora spp.; Germoplasm; Genetic diversity; RAPD-PCR.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132003000400005

Evaluation of Genetic Diversity amongthe Pakistani Wheat (Triticumaestivum L.) Lines through Random Molecular Markers
Provedor de dados: 52
Autores: Zeshan,Ali; Afzal,Muhammad; Alghamdi,Salem S.; Kettener,Karien; Ali,Mubashar; Mubushar,Muhammad; Ahmad,Shakeel.
ABSTRACT The presence of geneticdiversity is of great importance in improving wheat traits and developing strategies for optimal conservation of germplasm. Genetic diversity was assessed among common wheat cultivars using RAPD (Random Amplified Polymorphic DNA) markers at the Center of Agriculture, Biochemistry and Biotechnology (CABB), University of Agriculture, Faisalabad. RAPD primers were used among 14 Pakistani wheat cultivars, to screen the progenies and for the identification of the genes of interest. The polymorphic information content (PIC), was measured as the percentage of polymorphic fragments for all primers. A total of 583 bands(84% polymorphic) in all 14 wheat cultivars was amplified and discriminated all the wheat genotypes. The number of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Wheat; RAPD; Genetic diversity; PCoA; Cluster analysis.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100432

Genetic, morphological and spatial characterization of two populations of Mabea fistulifera Mart. (Euphorbiaceae), in different successional stages
Provedor de dados: 52
Autores: Goulart,Maíra Figueiredo; Ribeiro,Sérvio Pontes; Lovato,Maria Bernadete.
Two populations of Mabea fistulifera (Euphorbiaceae) located at Rio Doce State Park (Minas Gerais, Brazil) in places characterized as in different successional stages, were investigated through genetic and ecological traits. Twenty randomly chosen individuals from each population had its genetic data assessed by 43 RAPD markers and were also evaluated through five morphologic traits and its spatial distribution. Both Shannon's index and AMOVA reveled that most of the genetic variation was found between individuals within populations and about 9% of variation was located between populations. Moreover, populations differed genetically, but presented similar values of genetic diversity. Morphologic data showed differences between populations. Significant...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mabea fistulifera; Genetic diversity; RAPD; Tree architecture; Spatial distribution; Successional gradient.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000200015

Genetic divergence in sweet cassava cultivars using morphological agronomic traits and RAPD molecular markers
Provedor de dados: 52
Autores: Rimoldi,Fabrício; Vidigal Filho,Pedro Soares; Kvitschal,Marcus Vinícius; Gonçalves-Vidigal,Maria Celeste; Prioli,Alberto José; Prioli,Sônia Maria Alves Pinto; Costa,Tiago Ribeiro da.
In this work the genetic divergence among 14 sweet cassava cultivars was estimated by their morphological agronomic traits and RAPD molecular markers. The Tocher cluster analysis and the Nearest Neighbor Method were applied. The most dissimilar cultivars were Pão and Guaíra, Fécula Branca and Pão, and Pão and Caipira, while the most similar cultivar were the Fécula Branca and Branca 1, Branca 3 and Branca 1, and Guaíra and Branca 1. The Jaccard's coefficient showed that the most similar cultivars were Guaíra and Quarenta Quilos, while the most dissimilar were Branca 3 and Amarela da Rama Cinza. The divergence analysis indicated that promising crosses could be made between the Branca 3 cultivar and the Pão, Amarela 1, Fécula Branca and Amarela 2 cultivars...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Manihot esculenta; Multivariate analysis.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000600025

Molecular Insights into the Genetic Diversity of Garcinia cambogia Germplasm Accessions
Provedor de dados: 52
Autores: Tharachand,C; Immanuel Selvaraj,C; Abraham,Z.
ABSTRACTIn this work, the genetic relationship among twelveGarcinia cambogia (Gaertn.) Desr. accessions were evaluated using Random Amplified Polymorphic DNA markers. The samples were part of the germplasm collected and maintained at NBPGR Regional station, Thrissur, India. Out of thirty RAPD primers used for screening, seven primers produced a total of 128 polymorphic markers in twelve accessions. The Polymorphic Information Content (PIC) ranged from 0.28 (OPA18) to 0.37 (OPA9) and Marker Index (MI) ranged between 3.61 (OPA12) and 5.93 (OPA3) among the primers used. Jaccard's coefficient of genetic similarity ranged between 0.07 and 0.64. The dendrogram constructed based on the similarity matrix generated from the molecular and morphological data showed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Garcinia cambogia; Genetic diversity; Germplasm; Jaccard's coefficient; Malabar tamarind.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000500765

SSR-Marker assisted evaluation of Genetic Diversity in Mungbean ( Vigna radiata (L.) Wilcezk) genotypes
Provedor de dados: 52
Autores: Kaur,Gunnjeet; Joshi,Arunabh; Jain,Devendra.
ABSTRACT Mungbean (Vigna radiata (L.) Wilczek) also known as green gram is an important source of protein in the category of food legumes. In the present study, SSR marker is used to analyze the genetic diversity amongst 23 genotypes of mungbean. Out of a total of 10 primers used for SSR analysis revealed generation of 15 alleles. The number of alleles per locus ranged from one (CEDG006, CEDG010, CEDG050, CEDG088, CEDG092 and CEDG232) to three (CEDG 214), with an average of 1.5 allele per primer. The index for expected heterozygosity was 0.29 ranging from 0.15 to 0.49 revealed a deficit in heterozygosity. The size of amplification products varied in case of each primer and the range was found to be 100 bp to 190 bp. 13 out of 15 alleles were found...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Mungbean; SSR Marker assisted selection.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100400

Genetic variability of three natural populations of Maytenus aquifolium (Celesteraceae) from Telêmaco Borba, Paraná, Brazil
Provedor de dados: 52
Autores: Sahyun,Sandra Aparecida; Ruas,Eduardo Augusto; Ruas,Claudete de Fátima; Medri,Cristiano; Souza,José Roberto Pinto de; Johansson,Loana Aparecida Pereira da Silva; Miranda,Luíz Vicente; Ruas,Paulo Maurício.
Three populations of Maytenus aquifolium from Monte Alegre farm, Telemaco Borba county, Paraná, Brazil were analyzed by RAPD markers. A total of 13 primers were applied wich yielded 283 well amplified markers in all the studied populations (Mortandade, Vila Preta and Trinita), producing different values of gene diversity and polymorphic loci. The analysis of molecular variance (AMOVA) indicated that 21.77% of the genetic variation was among the population. Pairwise F ST analysis showed that the most divergent populations were closer geographically, demonstrating that other factors such as different soil types could explain this variation. Bayesian analysis for K number of clusters and the Principal Coordinate indicated that these three populations were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Genetic diversity; Maytenus aquifolium; RAPD; Populations.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000500005

HIV subtype, epidemiological and mutational correlations in patients from Paraná, Brazil
Provedor de dados: 55
Autores: Silva,Monica Maria Gomes da; Telles,Flavio Queiroz; Cunha,Clovis Arns da; Rhame,Frank S.
OBJECTIVE: Analyze patients with HIV infection from Curitiba, Paraná, their epidemiological characteristics and HIV RAM. METHODS: Patients regularly followed in an ID Clinic had their medical data evaluated and cases of virological failure were analyzed with genotypic report. RESULTS: Patients with complete medical charts were selected (n = 191). Demographic and clinical characteristics were compared. One hundred thirty two patients presented with subtype B infection (69.1%), 41 subtype C (21.5%), 10 subtype F (5.2%), 7 BF (3.7%) and 1 CF (0.5%). Patients with subtype B infection had been diagnosed earlier than patients with subtype non-B. Also, subtype B infection was more frequent in men who have sex with men, while non-B subtypes occurred more...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV; Aids; Subtypes; Subtype B; Subtype non-B; Genetic diversity; Antiretroviral; Brazil.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702010000500012

Molecular characterization of the first fluoroquinolone resistant strains of Streptococcus agalactiae isolated in Brazil
Provedor de dados: 55
Autores: Barros,Rosana Rocha; Kegele,Fabíola Cristina Oliveira; Paula,Geraldo Renato de; Brito,Monique Araújo de; Duarte,Rafael Silva.
Three isolates of Streptococcus agalactiae, recovered from residents of the metropolitan area of Rio de Janeiro with significant bacteriuria, were found to be resistant to levofloxacin. Determination of the minimal inhibitory concentration (MIC) confirmed one isolate as intermediate and two as resistant to levofloxacin. No reduction in levofloxacin MIC was observed with reserpine, indicating that resistance was not caused by an efflux mechanism. Typical point mutations were observed in the quinolone resistance determinant region of gyrA and parC. Other point mutations in parC generated novel altered codons: Ser80→Pro in the intermediate resistance isolate, and Gly128→Asp in a resistant isolate. Through molecular modeling, it was possible to observe that...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Fluoroquinolone resistance; Bacteriuria; Genetic diversity; Streptococcus agalactiae.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702012000500013

In vitro and in vivo antitumor activity of crude extracts obtained from Brazilian Chromobacterium sp isolates
Provedor de dados: 56
Autores: Menezes,C.B.A.; Silva,B.P.; Sousa,I.M.O.; Ruiz,A.L.T.G.; Spindola,H.M.; Cabral,E.; Eberlin,M.N.; Tinti,S.V.; Carvalho,J.E.; Foglio,M.A.; Fantinatti-Garboggini,F.
Natural products produced by microorganisms have been an important source of new substances and lead compounds for the pharmaceutical industry. Chromobacterium violaceum is a Gram-negative β-proteobacterium, abundant in water and soil in tropical and subtropical regions and it produces violacein, a pigment that has shown great pharmaceutical potential. Crude extracts of five Brazilian isolates of Chromobacterium sp (0.25, 2.5, 25, and 250 µg/mL) were evaluated in an in vitro antitumor activity assay with nine human tumor cells. Secondary metabolic profiles were analyzed by liquid chromatography and electrospray ionization mass spectrometry resulting in the identification of violacein in all extracts, whereas FK228 was detected only in EtCE 308 and EtCE 592...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chromobacterium sp; Antitumor activity; Secondary metabolites; Genetic diversity; Violacein.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2013000100065

Sequence analysis of the 5′ third of glycoprotein C gene of South American bovine herpesviruses 1 and 5
Provedor de dados: 56
Autores: Traesel,C.K.; Bernardes,L.M.; Spilki,F.R.; Weiblen,R.; Flores,E.F.
Bovine herpesviruses 1 (BoHV-1) and 5 (BoHV-5) share high genetic and antigenic similarities, but exhibit marked differences in tissue tropism and neurovirulence. The amino-terminal region of glycoprotein C (gC), which is markedly different in each of the viruses, is involved in virus binding to cellular receptors and in interactions with the immune system. This study investigated the genetic and antigenic differences of the 5′ region of the gC (5′ gC) gene (amino-terminal) of South American BoHV-1 (n=19) and BoHV-5 (n=25) isolates. Sequence alignments of 374 nucleotides (104 amino acids) revealed mean similarity levels of 97.3 and 94.2% among BoHV-1 gC (gC1), respectively, 96.8 and 95.6% among BoHV-5 gC (gC5), and 62 and 53.3% between gC1 and gC5....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: GC amino-terminal; BoHV; Genetic diversity; Epitope prediction; Virus neutralization.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000500470

Genetic analysis of Escherichia coli strains carrying enteropathogenic Escherichia coli (EPEC) markers, isolated from children in Rio de Janeiro city, Brazil
Provedor de dados: 58
Autores: Regua-Mangia,Adriana Hamond; Gomes,Tânia Aparecida Tardelli; Andrade,João Ramos Costa; Vieira,Mônica Aparecida Midolli; Gonzalez,Alice Gonçalves Martins; Zahner,Viviane; Irino,Kinue; Teixeira,Lúcia Martins.
In the present study, 47 enteropathogenic Escherichia coli strains identified according to serotyping, presence of eae, bfp and EAF sequences, adherence phenotype and ability to induce attaching-effacing lesions were analyzed by pulsed-field gel electrophoresis (PFGE), multilocus enzyme electrophoresis (MLEE), and the presence of LEE genes (eae, espA, espB, tir) as well as the respective alleles. Amplification of LEE genes subtypes revealed 18 different pathotypes. Typing of the eae gene showed that most strains contained nontypable intimin (42%) followed by beta (35%), gamma and alpha genes (12% each). PFGE analysis revealed a variable degree of polymorphism among isolates and, in general, no clear correlation was observed among PFGE profiles and the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Enteropathogenic Escherichia coli; Genetic diversity; Virulence markers.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500013

Variability of the conserved V3 loop tip motif in HIV-1 subtype B isolates collected from Brazilian and French patients
Provedor de dados: 58
Autores: Tomasini-Grotto,Rejane-Maria; Montes,Brigitte; Triglia,Denise; Torres- Braconi,Carla; Aliano-Block,Juliana; Zanotto,Paolo M. de A.; Pardini,Maria- Inèsde M. C.; Segondy,Michel.
The diversity of the V3 loop tip motif sequences of HIV-1 subtype B was analyzed in patients from Botucatu (Brazil) and Montpellier (France). Overall, 37 tetrameric tip motifs were identified, 28 and 17 of them being recognized in Brazilian and French patients, respectively. The GPGR (P) motif was predominant in French but not in Brazilian patients (53.5% vs 31.0%), whereas the GWGR (W) motif was frequent in Brazilian patients (23.0%) and absent in French patients. Three tip motif groups were considered: P, W, and non-P non-W groups. The distribution of HIV-1 isolates into the three groups was significantly different between isolates from Botucatu and from Montpellier (P &lt; 0.001). A higher proportion of CXCR4-using HIV-1 (X4 variants) was observed in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HIV-1; V3 loop; Genetic diversity; Brazil; France.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300024

Genetic diversity of siderophore-producing bacteria of tobacco rhizosphere
Provedor de dados: 58
Autores: Tian,Fang; Ding,Yanqin; Zhu,Hui; Yao,Liangtong; Du,Binghai.
The genetic diversity of siderophore-producing bacteria of tobacco rhizosphere was studied by amplified ribosomal DNA restriction analysis (ARDRA), 16S rRNA sequence homology and phylogenetics analysis methods. Studies demonstrated that 85% of the total 354 isolates produced siderophores in iron limited liquid medium. A total of 28 ARDRA patterns were identified among the 299 siderophore-producing bacterial isolates. The 28 ARDRA patterns represented bacteria of 14 different genera belonging to six bacterial divisions, namely ?-, ?-, ?-Proteobacteria, Sphingobacteria, Bacilli,and Actinobacteria. Especially, ?-Proteobacteria consisting of Pseudomonas, Enterobacter, Serratia, Pantoea, Erwinia and Stenotrophomonasgenus encountered 18 different ARDRA groups....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Siderophore; Tobacco rhizosphere; ARDRA; RRNA sequencing.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200013

Sensitivity, specificity and comparison of three commercially available immunological tests in the diagnosis of Cryptosporidium species in animals
Provedor de dados: 58
Autores: Danišová,Olga; Halánová,Monika; Valenčáková,Alexandra; Luptáková,Lenka.
ABSTRACT The study was conducted to compare the specificity of immunological diagnostic methods used for the diagnosis of Cryptosporidium species capable of causing life-threatening infection in both immunosuppressed and immunocompetent patients. For the detection of Cryptosporidium species in 79 animals with diarrhoea, we used three Copro-antigen tests: RIDASCREEN® Cryptosporidium test, Cryptosporidium 2nd Generation (ELISA) and RIDA®QUICK Cryptosporidium. For immunoassays we used positive and negative samples detected by means of polymerase chain reaction and validated by sequencing and nested polymerase chain reaction to confirm the presence six different species of Cryptosporidium species. Prevalence of cryptosporidiosis in the entire group determined...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryptosporidium spp.; Immunology tests; Comparison; PCR; Genetic diversity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000100177

Molecular characterisation of Aspergillus flavus isolates from peanut fields in India using AFLP
Provedor de dados: 58
Autores: Singh,Diwakar; Radhakrishnan,T.; Kumar,Vinod; Bagwan,N.B.; Basu,M.S.; Dobaria,J.R.; Mishra,Gyan P.; Chanda,S.V.
Aflatoxin contamination of peanut, due to infection by Aspergillus flavus, is a major problem of rain-fed agriculture in India. In the present study, molecular characterisation of 187 Aspergillus flavus isolates, which were sampled from the peanut fields of Gujarat state in India, was performed using AFLP markers. On a pooled cluster analysis, the markers could successfully discriminate among the ‘A’, ‘B’ and ‘G’ group A. flavus isolates. PCoA analysis also showed equivalent results to the cluster analysis. Most of the isolates from one district could be clustered together, which indicated genetic similarity among the isolates. Further, a lot of genetic variability was observed within a district and within a group. The results of AMOVA test revealed that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Aflatoxin; AMOVA; ELISA; Genetic diversity; Groundnut; PCA.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300673

Antimicrobial resistance and genetic diversity of Escherichia coli isolated from humans and foods
Provedor de dados: 58
Autores: Melo,Daniela Benevides; Menezes,Ana Paula de Oliveira; Reis,Joice Neves; Guimarães,Alaíse Gil.
Antibiotic resistance has increased in recent years, raising the concern of public health authorities. We conducted a study of Escherichia coli isolates obtained from human and food samples to assess the prevalence of antimicrobial resistance and to determine the genotype and clonal relationship of 84 E. coli isolates (48 from humans and 36 from foods). An antimicrobial susceptibility test was performed using the disk diffusion method. Virulence factors were evaluated by multiplex PCR, and the clonal relationship among the resistant isolates was studied by Pulsed Field Gel Electrophoresis (PFGE). All isolates were susceptible to ceftriaxone. Overall, 26%, 20.2%, 15.4% and 6% of the isolates were resistant to tetracycline, ampicillin,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Escherichia coli; Antimicrobial resistance; Food contamination; Genetic diversity.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401165

16s-23S rDNA: polymorphisms and their use for detection and identification of Xylella fastidiosa strains
Provedor de dados: 58
Autores: Martinati,Juliana Camargo; Pacheco,Flávia Tereza Hansen; Miranda,Vitor Fernandes Oliveira de; Tsai,Siu Mui.
Strains of Xylella fastidiosa from several hosts (coffee, citrus, grape, almond, oleander, peach, plum, etc.) were characterized by analyzing the content of the nucleotide sequences of 16S-23S rDNA (coding for a small subunit ribosomal RNA) spacer region (ITS). Current methods for sequencing the ITS region yields partial sequences that do not contribute with significant information. According to this fact, new primers have been designed in order to obtain a complete sequence and facilitate the sequencing. The complete 16S-23S sequences from 08 strains were amplified through PCR using the primers designed in our lab. The 16S-23S sequences obtained were compared with 52 others sequences entries in GenBank database. The results revealed a higher level of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; 16S-23S rDNA; Intergenic spacer; Identification; Detection.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100033

Genetic diversity of the VP1/VP2 gene of canine parvovirus type 2b amplified from clinical specimens in Brazil
Provedor de dados: 58
Autores: Pereira,Cesar A. D.; Durigon,Edison Luiz.
We evaluated the genetic diversity in the VP1/VP2 gene of CPV type 2b isolates from symptomatic dogs in Brazil. A total of 21 isolates collected from 1990 through 1995 previously typed as CPV2b by PCR assay were studied. Overall we found a high degree of similarity among sequences from different CPV clinical isolates collected. Genetic analysis of this selected region gave no indication of a specific Brazilian parvovirus lineage.
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Genetic diversity; Canine parvovirus (CPV); VP1/VP2 gene.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000400014

Genetic characterization of isolates of the basidiomycete Agaricus blazei by RAPD
Provedor de dados: 58
Autores: Colauto,Nelson Barros; Dias,Eustáquio Souza; Gimenes,Marcos Aparecido; Eira,Augusto Ferreira da.
The genetic divergence of five isolates of Agaricus blazei was determined based on RAPD data. Results indicate that there is little genetic variability among the commercialized strains and that RAPD is a feasible and low cost technique that can be used to characterize this fungus.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Agaricus blazei; Basidiomycete; Genetic diversity; Mushroom; RAPD.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000200006

Arbuscular mycorrhizal fungal communities in the rhizosphere of a continuous cropping soybean system at the seedling stage
Provedor de dados: 58
Autores: Cui,Jiaqi; Bai,Li; Liu,Xiaorui; Jie,Weiguang; Cai,Baiyan.
Abstract Arbuscular mycorrhizae (AM) fungi play a crucial role in the growth of soybean; however, the planting system employed is thought to have an effect on AM fungal communities in the rhizosphere. This study was performed to explore the influence of continuous soybean cropping on the diversity of Arbuscular mycorrhizal (AM) fungi, and to identify the dominant AM fungus during the seedling stage. Three soybean cultivars were planted under two and three years continuous cropping, respectively. The diversity of AM fungi in the rhizosphere soil at the seedling stage was subsequently analyzed using polymerase chain reaction (PCR)-denaturing gradient gel electrophoresis (DGGE). The results showed that an increase in cropping years improved the colonization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Soybean; Continuous cropping; Arbuscular mycorrhizae; PCR-DGGE; Genetic diversity.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200240

Low Genetic Diversity Among Garlic (Allium sativum L.) Accessions Detected Using Random Amplified Polymorphic DNA (RAPD)
Provedor de dados: 117
Autores: Paredes C,Mario; Becerra V,Viviana; González A,María I.
Garlic (Allium sativum L.) is a species of vegetative propagation, showing high morphological diversity. Besides, its clones have specific adaptations to different agroclimatic regions. The objective of this study was to determine the genetic diversity of 65 garlic clones collected in Chile and introduced from different countries, by using RAPD (Random Amplified Polymorphic DNA). Fourty random primers of 10 mers generated a total of 398 bands with an 87% of polymorphism. Each primer amplified between two and 20 bands. The size of the fragments obtained fluctuated between 3200 and 369 bp. The results showed that the clones analyzed had a genetic similarity rate of 94%. In addition, 70% of them were clustered in one major group. However, in spite of that...
Tipo: Journal article
Palavras-chave: Germplasm; Genetic diversity; RAPD diversity.
Ano: 2008
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392008000100001

Genetic divergence of parents and F2 segregation in grain Amaranths
Provedor de dados: 118
Autores: Milan Pandey,Ram.
Twenty six accessions of grain Amaranths (Amaranthus hypochondriacus), including both indigenous and exotic introductions, were evaluated in the rabi season (winter crop, November-March). Based on D² analysis, the accessions were grouped into eleven clusters. Clusters I, II, and III had seven, four, and three accessions, respectively; clusters VII, VIII, IX and X had only one accession in each case. The accession in cluster V had the greatest divergence, closely followed by those of clusters IV and I. The maximum and minimum divergences were revealed between clusters VIII and XI and between II and VIL respectively. The pattern of clustering did not show any relationship with geographic origin. In a study of pattern of F2 segregation in 25 crosses involving...
Tipo: Journal article
Palavras-chave: Amaranthus hypochondriacus; D² statistics; Genetic diversity; Grain amaranths.
Ano: 2009
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202009000100007

Genetic variability in maize and teosinte populations estimated by microsatellites markers
Provedor de dados: 65
Autores: Terra,Tatiana de Freitas; Wiethölter,Paula; Almeida,Cícero Carlos de Souza; Silva,Sérgio Delmar dos Anjos e; Bered,Fernanda; Sereno,Maria Jane Cruz de Melo; Barbosa Neto,José Fernandes.
Wild species are important sources of genetic variability and may be exploited by breeding programs. Crosses between teosinte and maize occur freely and teosinte serves as genetic source of agronomic traits for introduction in maize. The objective of this study was to estimate genetic variability among and within maize and teosinte populations (Zea mays mexicana). Two sweet maize populations (BR400 and BR402), two common maize populations (Suwan and Pampa) and one teosinte population were analyzed using microsatellites markers. Results indicated that 64,5% of the variation was detected within the populations, suggesting the possibility of obtaining genetic progress by selection within each population. The analysis with 25 microsatellites loci enabled the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zea mays mays; Zea mays mexicana; Genetic diversity; Flooding tolerance; Molecular markers.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782011000200003

Analysis on genetic diversity of 51 Grape germplasm resources
Provedor de dados: 65
Autores: Yue,Qingchun; Zhang,Chenfei; Wang,Qinghao; Wang,Wenjing; Wang,Jinyang; Wu,Yueyan.
ABSTRACT:  The objective of this study is to research the genetic diversity of the ‘ Zuijinxiang ’ grape and its mutant breeding F1 plants, we screened the excellent mutant plants with potential breeding value. 50 mutated single plants obtained from 137Cs-γ irradiated ‘Zuijinxiang’ grape seeds were used as research objects, and SCoT molecular marker technology was used for genetic diversity and variation analysis, and clustering research was carried out. The results showed that: (1) 36 SCoT primers produced abundant polymorphisms, and the amplification results showed obvious bright bands, and the amplification efficiency and polymorphism rate were 100%. (2) A total of 221 bands were amplified by 36 primers, of which 175 were rich in polymorphism, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Grape; SCoT markers; Genetic diversity; Cluster analysis.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100406

Genetic divergence among Nellore breed herds raised in the South Region of Brazil
Provedor de dados: 65
Autores: Lopes,Jader Silva; Rorato,Paulo Roberto Nogara; Weber,Tomás; Araújo,Ronyere Olegário de; Everling,Dionéia Magda; Bresolin,Tiago; Dornelles,Mariana de Almeida; Breda,Fernanda Cristina.
The objective of this study was to evaluate the genetic divergence among Nellore breed animals raised in 45 farms in the Southern Region of Brazil. The characteristic studied was weaning weight adjusted to 205 days of life (P205), from 10,874 animals sired by 425 bulls and 7,629 cows, collected between 1976 and 2001, and distributed in the states of Rio Grande do Sul (1,499), Santa Catarina (2,332) and Paraná (7,043). The animals were distributed by cluster analysis in eight genetic divergent groups, enabling this technique to be applied to organize the matings in order to obtain heterotic effect. The herd/farm groups were formed through the hierarchical Ward method, using the direct (VGD) and maternal (VGM) breeding values predicted by the REML method....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cluster analysis; Genetic diversity; Multivariate analysis; Similarity; Ward hierarchical method.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000500020

Genetics of two marine shrimp hatcheries of the Pacific white shrimp Litopenaeus vannamei (Boone, 1931) in Pernambuco, Brazil
Provedor de dados: 65
Autores: Lima,Ana Patrícia Souza de; Silva,Suzianny Maria Bezerra Cabral da; Oliveira,Karine Kelly Cavalcanti; Maggioni,Rodrigo; Coimbra,Maria Raquel Moura.
The shrimp industry has grown significantly over the past 10 years in Brazil, especially the farmed production of the exotic Pacific white shrimp, Litopenaeus vannamei. In 2004, this industry was marked by a productivity crisis, which stirred interest towards genetic improvement of shrimp stocks. Shrimp breeders importation was banned in Brazil by a govern Normative Instruction in 1997, as a sanitary precaution. Since then, broodstock replacement in hatcheries has been based on domestic stocks, raising concerns on the decline of genetic diversity and if the existing diversity would allow effective genetic improvement programs. In the present research, genetic parameters such as number of alleles, effective allele number, expected and observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; Population genetics; Genetic diversity; Microsatellite.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000200011

Genetic variability in physic nuts cultivated in Northeastern Brazil
Provedor de dados: 65
Autores: Oliveira,Vanice Dias; Rabbani,Allivia Rouse Carregosa; Silva,Ana Veruska Cruz da; Lédo,Ana da Silva.
This research had as objective to characterize genetically individuals of physic nut cultivated in experimental areas in Sergipe, Brazil by means of RAPD molecular markers. Leaves of 40 individuals were collected and DNA was isolated using CTAB 2% method. Were used 30 primers RAPD for DNA amplification, and this data was used to estimate the genetic similarity among the pairs of individuals, using Jaccard coefficient, and group them out for the UPGMA method. Also, the genetic structure and diversity of the populations were assessed using AMOVA. Of the 100 fragments generated, 29 of were polymorphic. A similarity average of 0.54 among the individuals was found and the amplitude similarities varied from 0.18 to 1.00. One of them (U5) was unit clusters and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Jatropha curcas L.; RAPD; Genetic diversity.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013000600006

Morpho-agronomic characterization and analysis of genetic divergenceamong accessions of tomatoes (Solanum lycopersicum L.)
Provedor de dados: 65
Autores: Fernandes,Murilo de Oliveira; Bianchi,Paola Alvares; Silva,Lígia Renata Almeida da; Vianna,Larissa Souza; Santos,Eileen Azevedo; Moulin,Monique Moreira.
ABSTRACT:  The collection and characterization of tomato germplasm are of relevant importance for agriculture. This study aimed to collect and characterize, by way of morphological description and statistical tools for the composition of groups, tomato accesses from the Southern and Serrana regions of the State of Espírito Santo, as well as to establish the tomato germplasm bank of the Ifes - Campus de Alegre. Thirty-seven accessions were collected from different commercial locations of Espírito Santo. The experiment was conducted in the Ifes Campus de Alegre in the Agroecology sector. For the morpho-agronomic characterization, sixteen essential descriptors were used, nine quantitative and seven qualitative. In the color of the ripe fruit, there was the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Germplasm; Genetic diversity; Qualitative and quantitative characters.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018001100400

Genetic parameters, phenotypic, genotypic and environmental correlations and genetic variability on sunflower in the Brazilian Savannah
Provedor de dados: 65
Autores: Lira,Ellen Grippi; Amabile,Renato Fernando; Fagioli,Marcelo; Montalvão,Ana Paula Leite.
ABSTRACT: Sunflower (Helianthus annuus L.) is an annual crop that stands out for its production of high quality oil and for an efficient selection, being necessary to estimate the components of genetic and phenotypic variance. This study aimed to estimate genetic parameters, phenotypic, genotypic and environmental correlations and genetic variability on sunflower in the Brazilian Savannah, evaluating the characters grain yield (YIELD), days to start flowering (DFL) based on flowering date in R5, chapter length (CL), weight of a thousand achenes (WTA), plant height (H) and oil content (OilC) of 16 sunflower genotypes. The experiment was conducted at Embrapa Cerrados, Planaltina, DF, situated at 15º 35’ 30”S latitude, 47º 42’ 30”W longitude and 1.007m above...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Genetic resources; Genotypic correlations; Helianthus annuus L.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000800402

Phenotypic and molecular characterization of corn hybrids released from 1980 to 2000
Provedor de dados: 65
Autores: Bispo,Noryam Bervian; Tessele,Carolina; Barbosa Neto,José Fernandes.
Genetic variability is the basis of plant breeding. In corn, a large and diverse genetic pool permits manipulation of different genotypes, but the genetic diversity of tropical germplasm has been little studied. With the objective of analyzing the amplitude of the genetic variability in 15 hybrids released in different periods by three different seed companies, phenotypic and molecular characterization was carried out in three environments and at two plant densities. The phenotypic assessment showed great similarity among the hybrids, suggesting that the breeding programs have developed genotypes with similar phenotypic traits. The dendogram of the molecular analysis showed clustering according with the different companies suggesting that each corn...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Corn germplasm; Genetic diversity; Breeding programs.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782009000700002

Genetic variability in elite barley genotypes based on the agro-morphological characteristics evaluated under irrigated system
Provedor de dados: 64
Autores: Amabile,Renato Fernando; Faleiro,Fábio Gelape; Capettini,Flávio; Peixoto,José Ricardo; Sayd,Ricardo Meneses.
ABSTRACT Recently, researches have shown that the Brazilian savannah has a great potential to supply the demand for barley grains. The purpose of this study was to assess the genetic variability in 39 elite barley (Hordeum vulgare L.) genotypes based on the agro-morphological traits of a crop irrigated in the savannah system. An irrigation experiment in the design of complete randomized block with four replicates was conducted at Federal District - Brazil. The evaluated traits were: distance from the last knot to the rachis, distance from the flag leaf to rachis, spike length, number of grains by ear, flag leaf area, plant height, silking, lodging, grain yield, thousand-seed weight, protein content and grain commercial classification. After using analysis...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic diversity; Hordeum vulgare L.; Genetic resources; Savannah.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542017000200147