Consulta: subjectFacets:"Immune response"
Registros recuperados: 60
Data/hora: 11/06/2024 06:57:58

Sistema de defensa de las larvas de gallina ciega contra los hongos entomopatógenos.
Provedor de dados: 32
Autores: Enríquez Vara, Jhony Navat.
Las larvas de gallina ciega son una plaga importante en cultivos de gramíneas en México. Dentro de las estrategias de manejo de esta plaga se ha considerado el uso del control microbiano con hongos entomopatógenos. Sin embargo, se han observado variaciones en la susceptibilidad de las especies de gallina ciega contra los hongos entomopatógenos. Se considera que uno de los factores importantes responsables de esta variación es el sistema de defensa (barreras inmunitarias y no inmunitarias) específicos de cada hospedero. Tomando como modelo de estudio a larvas gallina ciega de las especies Phyllophaga polyphylla y Anomala cincta, se estudió: a) si las variaciones de supervivencia entre ambas especies de gallina ciega contra Metarhizium pingshaense y...
Palavras-chave: Ecoimmunología; Melolonthinae; Rutelinae; Metarhizium pinshaense; Beauveria bassiana; Respuesta inmunitaria; Barreras no inmunitarias; Ecoimmunology; Immune response; Non-immune barrier; Entomología y Acarología; Doctorado.
Ano: 2013
URL: http://hdl.handle.net/10521/2320

Efecto de arginina y vitamina E en la respuesta inmunitaria de pollos de engorda vacunados e infectados experimentalmente con Eimeria spp.
Provedor de dados: 32
Autores: Pérez Carbajal, Carlos.
Este experimento se llevó a cabo para analizar los efectos inmunomodulatorios de arginina (ARG) y vitamina E (VE) en pollos de engorda infectados con coccidias. Un total de 300 pollos, vacunados contra coccidiosis (Coccivac®-B) al primer día de edad, fueron divididos en seis grupos con la finalidad de evaluar tres niveles de ARG en el alimento (NARG, 1.44%; MARG, 1.74%; HARG, 2.04%) y dos niveles de VE (40 u 80 UI / kg de alimento, VE40 y VE80, respectivamente) en un diseño factorial 3 x 2. Los pollos se criaron en piso y se les proporcionó agua y una dieta a base de maíz y pasta de soya ad libitum. Al d 14, todos los pollos se infectaron oralmente con un cultivo de diferentes especies del género Eimeria (E. acervulina, E. maxima y E. tenella). A los 9,...
Palavras-chave: Pollos de engorda; Arginina; Vitamina E; Respuesta inmunitaria; Broilers; Arginine; Vitamin E; Immune response; Maestría; Ganadería.
Ano: 2010
URL: http://hdl.handle.net/10521/63

Efecto de arginina y vitamina E en la respuesta inmunitaria de pollos de engorda vacunados e infectados experimentalmente con Eimeria spp.
Provedor de dados: 32
Autores: Pérez Carbajal, Carlos.
Este experimento se llevó a cabo para analizar los efectos inmunomodulatorios de arginina (ARG) y vitamina E (VE) en pollos de engorda infectados con coccidias. Un total de 300 pollos, vacunados contra coccidiosis (Coccivac®-B) al primer día de edad, fueron divididos en seis grupos con la finalidad de evaluar tres niveles de ARG en el alimento (NARG, 1.44%; MARG, 1.74%; HARG, 2.04%) y dos niveles de VE (40 u 80 UI / kg de alimento, VE40 y VE80, respectivamente) en un diseño factorial 3 x 2. Los pollos se criaron en piso y se les proporcionó agua y una dieta a base de maíz y pasta de soya ad libitum. Al d 14, todos los pollos se infectaron oralmente con un cultivo de diferentes especies del género Eimeria (E. acervulina, E. maxima y E. tenella). A los 9,...
Palavras-chave: Pollos de engorda; Arginina; Vitamina E; Respuesta inmunitaria; Broilers; Arginine; Vitamin E; Immune response; Maestría; Ganadería.
Ano: 2010
URL: http://hdl.handle.net/10521/63

The Mathematical Biology of Human Infections
Provedor de dados: 7
Autores: Nowak, Martin A; Institute for Advanced Study; nowak@ias.edu.
Humans are constant victims of infectious diseases. Biomedical research during this century has led to important insights into the molecular details of immune defense. Yet, many questions relating to disease require a quantitative understanding of the complex systems that arise from the nonlinear interactions between populations of immune cells and infectious agents. Exploration of such questions has lead to a newly emerging field of mathematical biology describing the spread of infectious agents both within and between infected individuals. This essay will discuss simple and complex models of evolution, and the propagation of virus and prion infections. Such models provide new perspectives for our understanding of infectious disease and provide guidelines...
Tipo: Peer-Reviewed Reports
Palavras-chave: CTL response; HIV; Antivirals; Epidemiology; Immune response; Infectious diseases; Mathematical biology; Models of evolution; Prions; Resistance; Treatment; Viruses.
Ano: 1999

The molecular characterization of a cyclophilin A from Chinese mitten crab Eriocheir sinensis and the antifungal activity of its recombinant protein
Provedor de dados: 69
Autores: Wang,Mengqiang; Zhang,Daoxiang; Wang,Lingling; Gai,Yunchao; Zhou,Zhi; Zhang,Huan; Song,Linsheng.
Background: Cyclophilin A (CypA), a receptor for the immunosuppressive agent cyclosporin A (CsA), is a cis-trans peptidyl-prolyl isomerase (PPIase) which accelerates the cis-trans isomerization of prolyl-peptide bonds and interacts with a variety of proteins to regulate their activities. Results: The full-length cDNA of crab Eriocheir sinensis CypA (EsCypA) was cloned by EST and RACE technique. The complete sequence of EsCypA cDNA contained a 5' untranslated region (UTR) of 50 bp, a 3&amp;#146; UTR of 233 bp with a polyA tail, and an open reading frame (ORF) of 495 bp encoding a polypeptide of 164 amino acids with the predicted molecular weight of 17.36 kDa. The deduced amino acid sequence of EsCypA contained two highly conserved signature sequences of...
Tipo: Journal article
Palavras-chave: Antifungal activity; Cyclophilin A; Eriocheir sinensis; Immune response; PPIase activity.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582013000200002

Spatiotemporal expression of MYD88 gene in pigs from birth to adulthood
Provedor de dados: 74
Autores: Gan,LiNa; Qin,WeiYun; Wu,Sen; Wu,ShengLong; Bao,WenBin.
Abstract MYD88 plays an important role in the immune response against infections. To analyze MYD88 gene expression during different stages of pig development, we used real-time PCR. MYD88 was seen expressed in all tissues examined. MYD88 expression in spleen, lungs, and thymus reached its highest value from 7 to 14 days of age and decreased thereafter. Expression in lymph nodes was high until 28 days of age and then it declined after weaning, with stable low levels in adult pigs. MYD88 expression was high before 35 days of age in the small intestine (duodenum, jejunum, and ileum), where it reached its highest value from 7 to 14 days of age. MYD88 expression in the small intestine declined post-weaning and remained relatively low during adulthood. The...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: MYD88; Pig; Developmental expression; Immune response.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572018000100119

Characterization and expression analysis of chymotrypsin after bacterial challenge in the mud crab, Scylla paramamosain
Provedor de dados: 74
Autores: Gong,Jie; Xie,Yinjie; Yu,Kun; Yang,Ya'nan; Huang,Huiyang; Ye,Haihui.
Chymotrypsin is one of the serine proteases families that have various biological functions. A chymotrypsin gene was isolated from hepatopancreas of the mud crab, Scylla paramamosain (designated SpCHY) in this study. The full-length cDNA of SpCHY contained 942 nucleotides with a polyadenylation sequence and encoded a peptide of 270 amino acids with a signal peptide of 17 amino acids. The SpCHY gene contains seven exons, six introns, a TATA box and several transcription factor binding sites that were found in 5'-promoter region which is 1221 bp in length-time quantitative PCR analysis indicated that the expression level of SpCHY mRNA in hepatopancreas was significantly higher than that in other tissues. Immunocytochemistry and in situ hybridization...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chymotrypsin; Scylla paramamosain; Immune response; Immunocytochemistry; In situ hybridization.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572014000300010

Expression profile of genes associated with mastitis in dairy cattle
Provedor de dados: 74
Autores: Fonseca,Isabela; Silva,Priscila Vendramini; Lange,Carla Christine; Guimarães,Marta F.M.; Weller,Mayara Morena Del Cambre Amaral; Sousa,Katiene Régia Silva; Lopes,Paulo Sávio; Guimarães,José Domingos; Guimarães,Simone E.F.
In order to characterize the expression of genes associated with immune response mechanisms to mastitis, we quantified the relative expression of the IL-2, IL-4, IL-6, IL-8, IL-10, IFN-γ and TNF-α genes in milk cells of healthy cows and cows with clinical mastitis. Total RNA was extracted from milk cells of six Black and White Holstein (BW) cows and six Gyr cows, including three animals with and three without mastitis per breed. Gene expression was analyzed by real-time PCR. IL-10 gene expression was higher in the group of BW and Gyr cows with mastitis compared to animals free of infection from both breeds (p &lt; 0.05). It was also higher in BW Holstein animals with clinical mastitis (p &lt; 0.001), but it was not significant when Gyr cows with and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokine; Gene expression; Immune response; Real-time PCR.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000400018

Etude de la réponse immunitaire chez l'huître Crassostrea gigas : caractérisation et fonctions de protéines de reconnaissance aux LPS et d'effecteurs antimicrobiens
Provedor de dados: 5
Autores: Gonzalez, Marcelo.
Research on the immune response of oysters is motivated by the problems of infectious diseases potentially found in the farms. Over the course of the last few years, the development of genomic approaches has allowed us to make headway on characterising genes involved in the cup oyster's (Crassostrea gigas) innate immunity, particularly thanks to various programmes for analysing transcriptome, of type EST (Express Sequence Tag) and subtractive banks. Within the context of this work, over the course of which we have used the data from genomic research and we have also developed biochemical approaches, we were particularly interested in studying the proteins involved in reconnaissance reactions to LPS as well as to antimicrobial effectors. In an earlier part,...
Tipo: Text
Palavras-chave: Defense peptides; Polysaccharides; LPS; Hemocytes; Immune response; Crassostrea gigas; Shellfish culture; LPS; Défensine; Peptides antimicrobiens; LBP; BPI; Superoxide dismutase; Hémocytes; Crassostrea gigas; Huître; Mollusque; Immunité.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/these-2098.pdf

Selection and characterization of potential probiotic bacteria for Litopenaeus stylirostris shrimp hatcheries in New Caledonia
Provedor de dados: 5
Autores: Pham, Dominique; Ansquer, Dominique; Chevalier, Anne; Dauga, Clement; Peyramale, Aude; Wabete, Nelly; Labreuche, Yannick.
In New Caledonia, shrimp hatcheries are confronted with mass mortality in the larval stages, a phenomenon poorly understood as no specific causative agent has been identified. This has resulted in an excessive use of prophylactic antibiotics, although their adverse effects in aquaculture are notorious. The present work was thus aimed at selecting potential probiotic strains for penaeid hatcheries.  From a pool of more than 400 marine bacterial isolates sampled from the local marine environment seven strains exhibited in vitro antagonistic activity towards Vibrio harveyi. These isolates were characterized both phenotypically and genotypically using a biochemical approach and 16S rDNA sequencing. Six out of these seven strains were found to belong to the...
Tipo: Text
Palavras-chave: Shrimp; Litopenaeus stylirostris; Probiotic; Hatchery; Pseudoalteromonas; Immune response.
Ano: 2014
URL: http://archimer.ifremer.fr/doc/00190/30129/28591.pdf

A relationship between antimicrobial peptide gene expression and capacity of a selected shrimp line to survive a Vibrio infection
Provedor de dados: 5
Autores: De Lorgeril, Julien; Gueguen, Yannick; Goarant, Cyrille; Goyard, Emmanuel; Mugnier, Chantal; Fievet, Julie; Piquemal, D; Bachere, Evelyne.
Understanding of antimicrobial defence mechanisms of penaeid shrimp should help in the design of efficient strategies for the management and disease control in aquaculture. In this study, we have specifically analysed the expression in circulating hemocytes of antimicrobial peptides (AMPs) encoding genes, such as PEN2 and PEN3, ALF, crustin, lysozyme and a putative cysteine-rich peptide. We evidenced a relationship between the level of expression of some AMPs and the successful response of the shrimp, Litopenaeus stylirostris, to circumvent a pathogenic Vibrio penaeicida infection. Additionally, significant differences in some AMP transcript amounts are evidenced between control, non-selected shrimp line and the third generation breeding of shrimp selected...
Tipo: Text
Palavras-chave: Real time PCR; Cysteine rich peptide; Crustin; Anti LPS factor; Lysozyme; Penaeidins; Immune response; Vibrio penaeicida; Penaeid; Decapoda; Crustacean.
Ano: 2008
URL: http://archimer.ifremer.fr/doc/2008/publication-4524.pdf

Stress response of farmed European abalone reveals rapid domestication process in absence of intentional selection
Provedor de dados: 5
Autores: Lachambre, Sebastien; Day, Rob; Boudry, Pierre; Huchette, Sylvain; Rio-cabello, Antoine; Fustec, Tirnothee; Roussel, Sabine.
Farming, and thus the domestication of Haliotis tuberculata, began recently. We compared the responses of unselected farmed and wild abalone to stressors that occur on farms. The aim was to determine if the farm environment had induced differences in the behavioural or physiological performances of the abalone. Thirty hatchery-born 3.5 year-old abalone and thirty wild ones were reared under standard farm conditions for 6 months and characterised for 19 traits related to growth, survival, behaviour and immunology. Behavioural and immunological responses to stressors differed between the two stocks. Farmed abalone retracted and swivelled less in reaction to a finger contact. Phagocytosis efficiency was reduced by shaking in abalone from both origins, but the...
Tipo: Text
Palavras-chave: Abalone aquaculture; Domestication syndrome; Behavioural adaptation; Responses to stress; Immune response; Farm stressors.
Ano: 2017
URL: http://archimer.ifremer.fr/doc/00395/50598/53725.pdf

Utilisation de bactéries lactiques probiotiques pour prémunir les poissons d'élevage contre des vibrions pathogènes
Provedor de dados: 5
Autores: Lamari, Faouzi.
Diseases cause major production losses in fish farms. Bacteria of the genus Vibrio are known as opportunistic pathogens, and have been associated with mass-mortality episodes. To cope with these disease outbreaks, fish farmers often resort to the use of antibiotics. However, preventive overuse has led to the emergence of resistant strains and frequent treatment failures. Probiotic lactic acid bacteria are increasingly used to improve the quality of seed production in aquaculture, while limiting antibiotic treatments. Microbial strains must fulfil several criteria to be evaluated as potential probiotics.In a first step, we selected and characterized 55 strains of lactic acid bacteria isolated from fish hatchery. The first selection criterion focused on the...
Tipo: Text
Palavras-chave: Probiotiques; Microflore intestinale; Réponse immunitaire; Stress oxydatif; Malformations vertébrales; Ossification; Oxidative stress; Vertebral malformations; Dicentrarchus labrax; Artemia; Vibrio; Probiotic; Intestinal microflora; Immune response.
Ano: 2014
URL: https://archimer.ifremer.fr/doc/00498/60943/64336.pdf

A proteomic study of resistance to Brown Ring disease in the Manila clam, Ruditapes philippinarum
Provedor de dados: 5
Autores: Smits, M.; Artigaud, Sebastien; Bernay, B.; Pichereau, Vianney; Bargelloni, L.; Paillard, Christine.
Marine mollusk aquaculture has more than doubled over the past twenty years, accounting for over 15% of total aquaculture production in 2016. Infectious disease is one of the main limiting factors to the development of mollusk aquaculture, and the difficulties inherent to combating pathogens through antibiotic therapies or disinfection have led to extensive research on host defense mechanisms and host-pathogen relationships. It has become increasingly clear that characterizing the functional profiles of response to a disease is an essential step in understanding resistance mechanisms and moving towards more effective disease control. The Manila clam, Ruditapes philippinarum, is a main cultured bivalve species of economic importance which is affected by...
Tipo: Text
Palavras-chave: Bivalve; Diseases; Resistance; Manila clam; Vibrio tapetis; Proteomics; Immune response.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00615/72670/71758.pdf

Is pallial mucus involved in Ostrea edulis defenses against the parasite Bonamia ostreae?
Provedor de dados: 5
Autores: Fernández-boo, Sergio; Gervais, Ophelie; Prado-alvarez, Maria; Chollet, Bruno; Claverol, Stéphane; Lecadet, Cyrielle; Dubreuil, Christine; Arzul, Isabelle.
Bonamia ostreae is an intrahemocytic parasite that has been responsible for severe mortalities in the flat oyster Ostrea edulis since the 1970́s. The Pacific oyster Crassostrea gigas is considered to be resistant to the disease and appears to have mechanisms to avoid infection. Most studies carried out on the invertebrate immune system focus on the role of hemolymph, although mucus, which covers the body surface of molluscs, could also act as a barrier against pathogens. In this study, the in vitro effect of mucus from the oyster species Ostrea edulis and C. gigas on B. ostreae was investigated using flow cytometry. Results showed an increase in esterase activities and mortality rate of parasites exposed to mucus from both oyster species. In order to...
Tipo: Text
Palavras-chave: Bonamia ostreae; Crassostrea gigas; Ostrea edulis; Flow cytometry; Immune response; Proteome.
Ano: 2020
URL: https://archimer.ifremer.fr/doc/00594/70602/68795.pdf

Effect of fish meal replacement by plant protein sources on non-specific defence mechanisms and oxidative stress in gilthead sea bream (Sparus aurata)
Provedor de dados: 5
Autores: Sitja Bobadilla, A; Pena Llopis, S; Gomez Requeni, P; Medale, Francoise; Kaushik, Sadasivam; Perez Sanchez, J.
Partial or total replacement of fish meal by a mixture of plant protein (PP) sources (corn gluten, wheat gluten, extruded peas, rapeseed meal and sweet white lupin) balanced with indispensable amino acids was examined in juvenile gilthead sea bream over the course of a 6-month growth trial. A diet with fish meal (FM) as the sole protein source was compared to diets with 50%, 75% and 100% of replacement (PP50, PP75, and PP100). The possible influence of diets on growth performance, plasma metabolites, gut integrity, liver structure, anti-oxidant and immune status was evaluated. Final body weight was progressively decreased with PP inclusion, but in PP50 and PP75-fed fish, feed efficiency (FE) was significantly improved and specific growth rates remained...
Tipo: Text
Palavras-chave: Gilthead sea bream; Cholesterol; Glutathione; Histopathology; Myeloperoxidase; Complement; Lysozyme; Respiratory burst; Immune response; Growth; Plant proteins; Fish meal.
Ano: 2005
URL: http://archimer.ifremer.fr/doc/2005/publication-3595.pdf

Saxitoxin Modulates Immunological Parameters and Gene Transcription in Mytilus chilensis Hemocytes
Provedor de dados: 5
Autores: Astuya, Allisson; Carrera, Crisleri; Ulloa, Viviana; Aballay, Ambbar; Nunez-acuna, Gustavo; Hegaret, Helene; Gallardo-escarate, Cristian.
Saxitoxin (STX) is a neurotoxin produced by dinoflagellates in diverse species, such as Alexandrium spp., and it causes paralytic shellfish poisoning (PSP) in humans after the ingestion of contaminated shellfish. Recent studies have suggested that the immune functions of bivalves could be affected by harmful algae and/or by their toxins. Herein, hemocytes are the main effector cells of the immune cellular response. In this study, we evaluated the response of hemocytes from the mussel Mytilus chilensis to STX exposure in a primary culture. Cell cultures were characterized according to size and complexity, while reactive oxygen species (ROS) production was evaluated using a dichlorofluorescein diacetate (DCFH-DA) assay. Finally, phagocytic activity was...
Tipo: Text
Palavras-chave: Saxitoxin; Hemocytes; Immune response; Paralytic shellfish poisoning; Reactive oxygen species (ROS).
Ano: 2015
URL: https://archimer.ifremer.fr/doc/00278/38954/73180.pdf

Vibriosis induced by experimental cohabitation in Crassostrea gigas: Evidence of early infection and down-expression of immune-related genes
Provedor de dados: 5
Autores: De Decker, Sophie; Saulnier, Denis.
The understanding of reciprocal interactions between Crassostrea gigas and Vibrio sp., whether these be virulent or avirulent, is vital for the development of methods to improve the health status of cultured oysters. We describe an original non-invasive experimental infection technique using cohabitation, designed to explore these interactions. Using real-time PCR techniques we examined the dynamics of virulent and avirulent Vibrio sp. in oyster hemolymph and tank seawater, and made a parallel study of the expression of four genes involved in oyster immune defense: Cg-BPI, Cg-EcSOD, Cg-IκB, Cg-TIMP.  No mortality occurred in control animals, but oysters put in cohabitation for 2–48 h with animals previously infected by two Vibrio pathogens suffered...
Tipo: Text
Palavras-chave: Oyster-Vibrio interactions; Pathogenesis; Immune response; Non-invasive experimental challenge; Real-time PCR.
Ano: 2011
URL: http://archimer.ifremer.fr/doc/00025/13604/10681.pdf

Measurement of IL-10 serum levels in BALB/c mice treated with beta-1,3 polyglucose or sulfadiazine and acutely infected by Toxoplasma gondii
Provedor de dados: 80
Autores: Picka,M. C. M.; Calvi,S. A.; Lima,C. R. G.; Santos,I. A. T.; Marcondes-Machado,J.
Acute infection by Toxoplasma gondii leads to suppression of cell-mediated immunity, facilitating chronic infection. One of the causes of immunosuppression is Interleukin-10 (IL-10) production. Glucan is used to stimulate phagocytosis. Our objective was to study IL-10 induction in male BALB/c mice with acute T. gondii BTU-2 strain infection, glucan immunostimulation, and sulfadiazine treatment. Animals were distributed into 7 groups: G1: infected with T. gondii; G2: infected with T. gondii and treated with sulfadiazine; G3: infected with T. gondii and immunostimulated with glucan; G4: infected with T. gondii, immunostimulated with glucan, and treated with sulfadiazine; G5: imunostimulated with glucan; G6: treated with saline; and G7: treated with...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Toxoplasma gondii; Interleukin-10; Glucan; Immune response; Sulfadiazine.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992005000400012

Benznidazole affects expression of Th1, Th17 and Treg cytokines during acute experimental Trypanosoma cruzi infection
Provedor de dados: 80
Autores: Gatto,Mariana; Oliveira,Larissa Ragozo Cardoso; Dias,Fernanda De Nuzzi; Araújo Júnior,João Pessoa; Lima,Carlos Roberto Gonçalves; Lordelo,Eliana Peresi; Santos,Rodrigo Mattos dos; Kurokawa,Cilmery Suemi.
Abstract  Background The present study evaluated the effect of treatment with benznidazole on mRNA expression of IFN-γ, IL-17, IL-10, TGF-β and FoxP3 in spleen and heart tissue of BALB/c mice in the acute phase of an experimental infection with Trypanosoma cruzi, strains JLP or Y.  Methods The mRNA expression of cytokines and parasite load were assessed by q-PCR. Dependent groups were compared using Student's paired t-test and independent groups were compared using Student's unpaired t-test.  Results Infection with the JLP or Y strains increased expression of IFN-γ in the heart and of IL-10 and IL-17 in the spleen and heart compared to uninfected animals. Treatment increased the expression of IFN-γ and decreased the expression of IL-17, IL-10, TGF- β and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chagas disease; Trypanosoma cruzi; Immune response; Cytokines; Q-PCR.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992017000100320

Immune response to Mycobacterium bovis-AN5 infection in genetically selected mice (selection IV-A)
Provedor de dados: 80
Autores: Cavaleiro,J. S.; Trindade,B. C.; Balderramas,H. A.; Haanwinckel,M. C.; Pinto,J. G. G.; Oliveira,S. L.; Paes,A. C.
Mice genetically selected for high (H) and low (L) antibody production (Selection IV-A) were used as murine experimental model. The aim of the present work was to evaluate the macrophagic activity and to characterize the immune response in Mycobacterium bovis-AN5 infected mice (3X10(7) bacteria). The response profile previously observed in such strains was not similar to that obtained during M. bovis infection; however, it corroborated works carried out using Selection I, which is very similar to Selection IV-A regarding infection by M. tuberculosis and Bacillus Calmette-Guérin (BCG). Considering bacterial recovery, L IV-A mice showed higher control of the infectious process in the lungs than in the spleen, whereas H IV-A mice presented more resistance in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune response; Mycobacterium bovis; Biozzi mice.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992007000300006

On the interference of clinical outcome on rabies transmission an perpetuation
Provedor de dados: 80
Autores: Brandão,PE.
Rabies is a viral zoonotic infectious disease that affects mammals and is caused by genotypes/species of the Lyssavirus genus (Rhabdoviridae, Mononegavirales), with the genotype 1 (classic rabies virus - RABV) being the most prevalent. Despite continuous efforts, rabies is still an incurable disease that causes thousands of deaths amongst humans worldwide. Due to a wide range of hosts and the different evolutionary paths of RABV in each host, several host-specific variants have arisen in an ongoing process. The result of RABV replication in nervous tissues may lead to two opposite clinical outcomes, i.e., paralytic/dumb form and encephalitic/furious one. The paralytic form creates dead-end hosts mainly amongst herbivores, while the furious form of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Rabies; Transmission; Disease ecology; Epidemiology; Immune response.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000200003

Immunogenicity of Toxoplasma gondii Heat Shock Protein 70
Provedor de dados: 19
Autores: Miller, C. M.; Smith, N. C.; Luton, K.; Dobbin, C. A.; Johnson, Alan M.
Palavras-chave: Toxoplasma gondii; Heat shock protein; Vaccination; Immune response.
Ano: 1999
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/325

Effects of Heavy Metal and Pollutants on the Non-special Immunity of the Shrimp and Crab.
Provedor de dados: 20
Autores: Ai, Chunxiang; Wang, Xiaojuan; Li, Shaojing; Wang, Guizhong; Lin, Qiongwu.
This paper attempted to review and evaluate existing information about the effects of heavy metal，ammonia-N，nitrite and the organic pollutants on the non-special immune response of the shrimp and crab, which providing theory for improving the self-immunity of shrimp and crab by meliorating cultural environment. In addition, it could provide information for further study on this field.
Tipo: Journal Contribution
Palavras-chave: Immune response; Heavy metals; Pollutants; Crabs; Prawns and shrimps.
Ano: 2008
URL: http://hdl.handle.net/1834/5871

Assessment of a probiotic Containing Bacillus Subtilis on the Performance and Gut Health of Laying Japanese Quails (Coturnix Coturnix Japonica)
Provedor de dados: 89
Autores: Manafi,M; Khalaji,S; Hedayati,M.
ABSTRACT The present study was carried out to determine the effects of the inclusion of a spore-forming probiotic (Bacillus subtilis) in laying Japanese quail diets as an alternative to growth-promoting antibiotics to help produce healthy eggs and meat. This experiment was conducted as a completely randomized design with three treatments (control, 0.05% bacitracin methylene disalicylate (BMD), or 0.1% Bacillus subtilis) of five replicates of 11 quails each. Feed intake and egg production were recorded daily on cage basis. Body weight was determined at the beginning and end of the trial (36 and 42 weeks). At the end of the experiment (42 weeks), antibodies against Newcastle disease and avian influenza, egg components, Haugh units, eggshell quality and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Japanese quails; Egg production; Gut health; Immune response.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400599

Etiology and immunology of infectious bronchitis virus
Provedor de dados: 89
Autores: Caron,LF.
Infectious bronchitis virus (IBV) of chickens is currently one of the main diseases associated with respiratory syndrome in domestic poultry, as well as with losses related to egg production. The etiological agent is a coronavirus, which presents structural differences in the field, mainly in the S1 spike protein. The immune response against this virus is complicated by the few similarities among serotypes. Environmental and management factors, as well as the high mutation rate of the virus, render it difficult to control the disease and compromise the efficacy of the available vaccines. Bird immune system capacity to respond to challenges depend on the integrity of the mucosae, as an innate compartment, and on the generation of humoral and cell-mediated...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Chicken Bronchitis; Immune response; Vaccine.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2010000200007

Effect of Stocking Density on the Performance and Immunity of 1- to 14-d- Old Broiler Chicks
Provedor de dados: 89
Autores: Qaid,M; Albatshan,H; Shafey,T; Hussein,E; Abudabos,AM.
ABSTRACT The current experiment was conducted to evaluate the effect of stocking density (SD) on the performance and immunity of 1- to 14-d-old broilers. A total of 1836 one-day-old Cobb 500 broilers were housed at four different SD (30, 60, 90 and 120 chicks/m2). Body weight gain (BWG), feed intake (FI) and feed conversion ratio (FCR) were estimated on d 2, 5, 8, 11 and 14. Relative lymphoid organ weights, maternal antibody titers against IBV, IBD and NDV, and stress indicators were estimated on d 3, 6, 9 and 12.The results indicated that age significantly (p&lt;0.001) affected the performance and immunity of broiler chicks. Stocking density significantly (p&lt;0.001) affected the performance and physiological stress indicators of broiler chicks, but not...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broilers; Immune response; Stocking density.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000400683

The Effects of Different Types of Antioxidants (Se, Vitamin E and Carotenoids) in Broiler Diets on the Growth Performance, Skin Pigmentation and Liver and Plasma Antioxidant Concentrations
Provedor de dados: 89
Autores: Karadas,F; Erdoğan,S; Kor,D; Oto,G; Uluman,M.
Abstract This study investigated the effects of the addition of different antioxidants to broiler diets on their live performance, liver antioxidant composition and concentrations, immune response, and meat and skin color. A total of 945 three-day-old Ross 308 broiler chicks of both genders were randomly allocated to one of nine dietary treatments (n=105), with three replicates 35 chicks per pen, as follows: T1: control (commercially available corn-and soybean-based broiler diet); T2: selenium (control+0.5 mg/kg Sel-PlexTMSe yeast); T3: vitamin E (control+200 mg/kg Kavimix-E-50 a-tocopherol acetate); T4: lutein (control+100 mg/kg 5% Lutein Beads XB); T5: lycopene (control+100 mg/kg 5% Lyco Beads XB);T6: canthaxanthin (control+25 mg/kg 10%...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Antioxidants; Carotenoids; Performance; Immune response; Pigmentation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000100101

Effects of Lactobacillus Probiotic, P22 Bacteriophage and Salmonella Typhimurium on the Heterophilic Burst Activity of Broiler Chickens
Provedor de dados: 89
Autores: Marietto-Gonçalves,GA; SMR,Curotto; Baptista,AAS; Donato,TC; Takahira,RK; Sequeira,JL; Andreatti Filho,RL.
Due to the constant evolution of industrial poultry production and the global emergence of bacterial resistance to antibiotics there has been an increasing interest in alternatives for the treatment of poultry salmonellosis, such as phage therapy and probiotics. The present study evaluated the effects of the oral administration of the bacteriophage P22 and of a probiotic, consisting of four Lactobacillus species, on the level of circulating heterophils containing a superoxide anion of one-day-old broilers challenged with Salmonella Typhimurium for seven days. It was concluded that the treatment with a probiotic with lactobacilli of broilers experimentally infected with Salmonella spp eliminates this pathogen by increasing the circulating levels of reactive...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonellosis; Immune response; Respiratory burst; Poultry.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2014000300005

Safety and efficacy of a salmonella gallinarum ΔcobSΔcbiA strain with potential to prevent chicken infections by salmonella gallinarum and salmonella enteritidis
Provedor de dados: 89
Autores: Paiva,JB; Penha Filho,RAC; Moura,BS; Berchieri Junior,A.
New vaccine design techniques have allowed the development of effective vaccine strains against Salmonella infections inwhich the risks of reversion to the wild type and virulence is null. The mutant strain Salmonella Gallinarum ΔcobSΔcbiA was previously shown to be avirulent in chickens. In this study, this strain was tested as a vaccine against Salmonella Gallinarum (SG) and S. Enteritidis (SE) infections, and its protection levels, safety and possible risks of reversion to virulence after vaccination of layers were evaluated. Birds were vaccinated at five days of age or at five and 25 days of age. At 45 days of age, brown and white layers were challenged with SG and SE wild strains, respectively. Two assays to test the possibility of reversion to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Salmonella vaccine; Attenuation; Reversion to virulence; Chicken; Immune response.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2012000200005

Passionflower supplementation in diets of Japanese qualis at rearing and laying periods
Provedor de dados: 96
Autores: Silva,Janaina Della Torre da; Gravena,Rodrigo Antonio; Marques,Rafael Henrique; Silva,Vanessa Karla; Hada,Fabricio Hirota; Moraes,Vera Maria Barbosa de; Malheiros,Ramon Diniz.
The objective of this study was to assess the effect of the inclusion of passionflower (Passiflora alata) in diets on performance, behavior, heterophil/lymphocyte ratio (H/L), corticosterone plasma levels (CPL) and immune response of quails. The study was carried out using 192 birds, distributed in randomized blocks with 4 diets (0, 125, 250 and 375 and 0, 250, 500 and 750 mg of passionflower/kg of feed for the rearing and laying period, respectively), 8 replicates and 6 birds per experimental unit. The rearing period began when birds were 28 days of age and it lasted for 15 days. In this phase, it was evaluated daily feed intake, daily weight gain, feed conversion, viability, tonic immobility time, intensity of injuries, behavior, heterophil/lymphocyte...
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Behavior; Corticosterone; Heterophil/lymphocyte ratio; Immune response; Stress; Tonic immobility.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010000700020

Hemolytic activity of alternative complement pathway as an indicator of innate immunity in pacu (Piaractus mesopotamicus)
Provedor de dados: 96
Autores: Biller-Takahashi,Jaqueline Dalbello; Takahashi,Leonardo Susumu; Marzocchi-Machado,Cleni Mara; Zanuzzo,Fabio Sabbadin; Sabioni,Rafael Estevan; Urbinati,Elisabeth Criscuolo.
The objective of this study was to evaluate the methodology to establish the hemolytic activity of alternative complement pathway as an indicator of the innate immunity in Brazilian fish pacu (Piaractus mesopotamicus), in addition to verifying the influence of β-glucan as an immunostimulant. Fish were fed with diets containing 0, 0.1 and 1% β-glucan, during seven days, and then inoculated with Aeromonas hydrophila. Seven days after the challenge, they were bled for serum extraction. The methodology consisted of a kinetic assay that allows calculating the required time for serum proteins of the complement to promote 50% lysis of a rabbit red blood cell suspension. The method developed in mammals was successfully applied for pacu and determined that the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: A. hydrophila; Β-glucan; Immune response; Pacu.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012000200001

Influence of fermented fish meal supplementation on growth performance, blood metabolites, and fecal microflora of weaning pigs
Provedor de dados: 96
Autores: Lee,Hyuk Jun; Choi,In Hag; Kim,Dong Hyeon; Joo,Young Ho; Kim,Sam Churl.
ABSTRACT This study was conducted to estimate the effect of dietary supplementation with fermented fish meal on growth performance, blood metabolites, and fecal microflora in weaning pigs. A total of 180 weaned pigs ((Landrace ×Yorkshire) × Duroc; with average body weight of 6.0 kg) were randomly distributed among three dietary treatments (0, 0.2, and 0.5% fermented fish meal) in three replicate pens (20 heads per pen) in a completely randomized trial over three weeks. Addition of fermented fish meal to weanling pig diets had a linear effect on average feed intake and a quadratic trend on final body weight, average daily gain, and gain:feed ratio throughout the whole period (but not initial body weight). Hematocrit, monocyte, immunoglobulin G, and blood...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Growth factor; Hormone; Immune response; Pig.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982017000500433

Use of probiotic-supplemented diet on a Pacific white shrimp farm
Provedor de dados: 96
Autores: Vieira,Felipe do Nascimento; Jatobá,Adolfo; Mouriño,José Luiz Pedreira; Buglione Neto,Celso Carlos; Silva,Jairo Sousa da; Seiffert,Walter Quadros; Soares,Mariana; Vinatea,Luis Alejandro.
ABSTRACT The objective of this study was to evaluate the use of a diet supplemented with Lactobacillus plantarum on performance, immunological parameters, and bacterial microbiota of the digestive tract of white leg shrimp (Litopenaeus vannamei). We used six earthen ponds (1.2 ha) stocked with 14 shrimp m−2 (3 g mean weight). Three ponds received a diet supplemented with L. plantarum and three were maintained as control. The survival and feed efficiency of shrimp fed the supplemented diet were greater than that observed in shrimp fed the control diet, with survival values of 83.02±6.12% and 74.65±9.07% and feed efficiencies of 117.97±4.45% and 104.46±7.30%, respectively. However, we observed no differences in weight gain. The intestines of shrimp fed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune response; Lactobacillus; Microbiology.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982016000500203

Serum concentration of acute phase proteins and cytokines in vaccinated pigs challenged with foot-and-mouth disease virus serotype O
Provedor de dados: 96
Autores: Lee,Kyung-Woo; Lee,Kwang-Nyeong; Lillehoj,Hyun S.; Park,Jong-Hyeon.
ABSTRACT The present study aimed to investigate the kinetics of acute phase proteins and cytokines in the serum of immunized or naive pigs following infection with a field isolate of foot-and-mouth disease (FMD) virus. Five-month-old SPF pigs were intramuscularly immunized with or without the 146S antigen (7.5 μg per head) purified from FMD virus (O/Andong/SKR/2010) and were subsequently challenged 30 days post-vaccination (dpv) with FMD virus on each footpad. Serum samples were obtained at 28 dpv and on days 1, 3, and 5 post-infection (dpi). At 28 dpv, the serum concentrations of C-reactive protein and tumor necrosis factor alpha (TNF-α) were higher in the immunized pigs compared with the non-immunized control pigs. Upon challenge, the levels of all...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: C-reactive protein; Immune response; Major acute phase protein; Tumor necrosis factor alpha.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100505

Effect of different sources of selenium on performance and characteristics of immune system of broiler chickens
Provedor de dados: 96
Autores: Shabani,Roozbeh; Fakhraei,Jafar; Yarahmadi,Hossein Mansoori; Seidavi,Alireza.
ABSTRACT An experiment was conducted based on a completely randomized design with 500 Ross 308 broiler chickens to investigate the effect of three different sources of selenium on performance and characteristics of immune system. Chickens were arranged in 10 treatments and five repetitions (10 chickens for each repetition). Ten treatments of experimental diet were considered in the study, including a basal diet (control); 0.5, 0.8, and 1.2 mg nano-selenium + basal diet (three treatments); 0.5, 0.8, 1.2 mg selenomethionine + basal diet; and 65, 80, and 100 mg vitamin E + selenium + basal diet. Findings suggested that adding selenomethionine (1.2 mg) to the basal diet resulted in a significant effect on feed conversion ratio and weight of broilers. In fact,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Broiler; Immune response; Immunoglobin; Selenium.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982019000100535

Humoral immune response of domestic dogs that received a single dose of rabies vaccine
Provedor de dados: 195
Autores: da Silva, Viviane Alcântara; Gamon, Thais Helena Martins; da Silva, Andréa de Cássia Rodrigues; Caporale, Graciane Maria Medeiros; Chaves, Luciana Botelho; Scheffer, Karin Corrêa.
The National Programme for Rabies Prevention aims to maintainimmunogenic protective levels in vaccinated animals, with titersof neutralizing antibodies (VNA) ≥ 0.5 IU / mL. The aim of thisstudy was to evaluate, according to age, race, and the periodof vaccination and blood collection, the immune response indogs that received a single dose of rabies vaccine virus in cellculture. In this study 432 samples received at the Pasteur Instituteof São Paulo in the triennium 2009-2011 were analyzed. Datawere analyzed and the evaluation of the VNA to rabies virus wasperformed by rapid fluorescent inhibition test foci (RFFIT). Of thetotal samples analyzed, 21.76% did not have protective titers.Among these, 67.02% samples were considered puppies andwhen the interval...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Neutralizing antibodies; Immune response; Dogs; Rabies; RFFIT; Anticorpos neutralizantes; Resposta imune; Cães; Raiva; RFFIT.
Ano: 2014
URL: http://www.revistamvez-crmvsp.com.br/index.php/recmvz/article/view/23105

Soybean protein concentrate in Pacific white shrimp reared in bioflocs: effect on health and vibrio challenge
Provedor de dados: 2
Autores: Schleder, Delano Dias; Jatobá, Adolfo; Silva, Bruno Corrêa da; Ferro, Daniel Paulo Damin; Seiffert, Walter Quadros; Vieira, Felipe do Nascimento.
Litopenaeus vannamei shrimp were reared in a bioflocs system and fed different levels of soybean protein concentrate as a replacement for fishmeal, and both immunological parameters of this marine shrimp and its resistance to Vibrio sp. infection (CPQBA 378-12 DRM01) were evaluated. Four different diets were formulated with 0, 33, 66 and 100 % of soybean protein concentrate as a substitute for fishmeal. Shrimp were reared in a biofloc system in twelve 800 L tanks (250 shrimp m-3) maintained at constant aeration and temperature. After 42 days, 36 animals (14.21 ± 0.89 g) per treatment were challenged with Vibrio sp. (1 x 105 CFU mL-1 – LD10), and hemolymph was collected before and after challenge to perform immunological assays (agglutination titer,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune response; Infection; Plant protein; Litopenaeus vannamei.
Ano: 2018
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/42570

Dietary lipid sources affect freshwater catfish jundiá, Rhamdia quelen, survival, when challenged with Aeromonas hydrophila - doi: 10.4025/actascianimsci.v35i4.19617
Provedor de dados: 2
Autores: Vargas, Rodrigo Javier; Universidade Federal de Santa Catarina (UFSC); Dotta, Geovana; Universidade Federal de Santa Catarina (UFSC); Mouriño, José Luiz; Universidade Federal de Santa Catarina (UFSC); Silva, Bruno Correia da; Universidade Federal de Santa Catarina (UFSC); Fracalossi, Débora Machado; Universidade Federal de Santa Catarina (UFSC).
The immune modulatory activity of diet long chain polyunsaturated fatty acids in fish has been previously demonstrated, although results for freshwater species are controversial.  This study evaluates the effect of different dietary lipids on freshwater catfish jundiá, Rhamdia quelen, survival and its non-specific response (phagocytosis) after being inoculated with the pathogen  Aeromonas hydrophila. Five diets were offered to jundiá fingerlings during 150 days prior to challenge: cod liver oil (FO), sunflower oil (SO), linseed oil (LO), canola oil (CO) and coconut oil (CNO). Accumulated mortality was significantly lower in fish fed FO and CNO diets and higher in fish fed LO. In spite of the highest values observed for phagocytotic activity in CNO-fed fish...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 5.06.03.00-0 mortality; Phagocytosis; Oils; Immune response Mortality; Phagocytosis; Oils; Immune response.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/19617

Auto-lysed yeast and yeast extract effects on dry matter intake, blood cells counts, IgG titer and gene expression of IL-2 in lactating dairy cows under heat stress
Provedor de dados: 2
Autores: Adili, Sogol; Sadeghi, Ali Asghar; Chamani, Mohamad; Shawrang, Parvin; Forodi, Farhad.
The objective of this study was to assess the effects of auto-lysed yeast and yeast extract on performance and immune responses of cows in hot climate in the early lactation period. Twenty five lactating dairy cows randomly assigned to 5 groups and 5 replicates. Cows received basal diet with or without auto-lysed yeast (20 or 40 g/d/head) or yeast extract (20 or 40 g/d/head) as on top-dressed. There were no differences for daily dry matter intake, milk production milk fat and the counts of red blood cells and white blood cells among treatments (p &amp;gt; 0.05). There were significant differences among treatments for immunoglobulin G (IgG) level, lymphocyte and neutrophil percentages. Yeast extract had no effect on IgG level, but auto-lysed yeast increased...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunoglobulin G; Immune response; Interleukin-2; Lymphocyte; Neutrophil immunoglobulin G; Immune response; Interleukin-2; Lymphocyte; Neutrophil.
Ano: 2020
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAnimSci/article/view/48425

High susceptibility of activated lymphocytes to oxidative stress-induced cell death
Provedor de dados: 42
Autores: Degasperi,Giovanna R.; Castilho,Roger F.; Vercesi,Anibal E.
The present study provides evidence that activated spleen lymphocytes from Walker 256 tumor bearing rats are more susceptible than controls to tert-butyl hydroperoxide (t-BOOH)-induced necrotic cell death in vitro. The iron chelator and antioxidant deferoxamine, the intracellular Ca2+ chelator BAPTA, the L-type Ca2+ channel antagonist nifedipine or the mitochondrial permeability transition inhibitor cyclosporin A, but not the calcineurin inhibitor FK-506, render control and activated lymphocytes equally resistant to the toxic effects of t-BOOH. Incubation of activated lymphocytes in the presence of t-BOOH resulted in a cyclosporin A-sensitive decrease in mitochondrial membrane potential. These results indicate that the higher cytosolic Ca2+ level in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell death; Free radicals; Immune response; Lymphopenia; Mitochondrial permeability transition; Spleen lymphocyte.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652008000100009

Effects of black seed (Nigella sativa), ginger (Zingiber officinale) and cone flower (Echinacea angustifolia) on the immune system of rainbow trout, Oncorhynchus mykiss
Provedor de dados: 45
Autores: Fadeifard,F.; Raissy,M.; Jafarian,M.; Boroujeni,H.Rouhi; Rahimi,M.; Faghani,M.
ABSTRACT This study was carried out to evaluate the effects of dietary essential oils of ginger (Zingiber officinale), black seed (Nigella sativa) and cone flower (Echinacea angustifolia) on the immune system of cultured rainbow trout, Oncorhynchus mykiss. A total of 360 fish were divided into 4 groups and 3 replicates each containing 30 fish. The fish were fed for 21 days with 1% of the herbal essential oils and with unsupplemented diet as the control group. Haematological and immune parameters including serum lysozyme, number of phagocytic bacteria, Hb, PCV, MCV, MCH, MCHC, number of RBC and WBC were studied. Treatments recorded enhancement in immune parameters compared to the control group. Significantly higher serum lysozyme level and phagocytic germs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Nigella sativa; Zingiber officinale; Echinacea angustifolia; Immune response; Oncorhynchus mykiss.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000100199

Hypoxia increases susceptibility of Pacific white shrimp to whitespot syndrome virus (WSSV)
Provedor de dados: 45
Autores: Lehmann,M.; Schleder,D.D.; Guertler,C.; Perazzolo,L.M.; Vinatea,L.
The present study aimed to evaluate the mortality, reactive oxygen species production (ROS) and total hemocyte counts (THC) of the marine shrimp Litopenaeus vannamei infected with the white spot syndrome virus (WSSV) at three levels of oxygen saturation. For this, 360 shrimp (20±2g) were distributed in 24 tanks (60L), divided in two groups (infected and non-infected), which were subjected to 30, 60 and 100% of dissolved oxygen saturation (in quadruplicate). During 96 hours after infection, daily hemolymph samples were collected for hemato-immunological parameter evaluation (THC and ROS) and dead animals were removed and computed to assess cumulative mortality rates. In the infected group, animals subjected to 100% saturation showed higher ROS production...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Litopenaeus vannamei; WSSV; Dissolved oxygen saturation; Immune response; Mortality.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352016000200397

Relationship between clinical symptomatology on the isolation of Salmonella Gallinarum of japanese quail (Coturnix coturnix)
Provedor de dados: 45
Autores: Rocha-e-Silva,R.C.; Cardoso,W.M.; Horn,R.V.; Cavalcanti,C.M.; Beleza,A.J.F.; Almeida,C.P.; Bezerra,W.G.A.; Carmo,C.C.; Nogueira,C.H.G.; Lima,B.P.; Marques,A.R.; Teixeira,R.S.C.
ABSTRACT Salmonella Gallinarum is capable of causing high mortality in birds of the order Galliformes. This study aimed to relate the presence of clinical signs with the recovery of Salmonella Gallinarum from organs and c loacal swabs of Japanese quails (Coturnix coturnix) experimentally infected. A total of 70 female quails were housed in a pair per cage and divided in two groups (IG: quails inoculated with 1.5x106 CFU of Salmonella Gallinarum Nalr/mL and CG: control group). After the inoculation, birds were evaluated three times a day to verify the presence of clinical signs. Birds that presented ruffled feathers, eyes closed and remained quiet in the cage were removed for euthanasia, as well as the same number of birds from the inoculated groups that...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immune response; Isolated; Mortality; Clinical signs.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000401187

Production and immune response of recombinant Hsp60 and Hsp70 from the salmon pathogen Piscirickettsia salmonis
Provedor de dados: 48
Autores: WILHELM,VIVIAN; SOZA,CRISTIÁN; MARTÍNEZ,RODRIGO; ROSEMBLATT,MARIO; BURZIO,LUIS O; VALENZUELA,PABLO D.T.
We have isolated and sequenced the genes encoding the heat shock proteins 60 (Hsp60) and 70 (Hsp70) of the salmon pathogen Piscirickettsia salmonis. The sequence analysis revealed the expected two open reading frames that encode proteins with calculated molecular weights of 60,060 and 70,400. The proteins exhibit a 70-80% homology with other known prokaryotic Hsp60 and Hsp70 sequences. The coding regions have been expressed in E. coli as thioredoxin fusion proteins. Both recombinant proteins were shown to elicit a humoral response when injected intraperitoneally in Atlantic salmon and also conferred protection to fish challenged with P. salmonis. The present data will facilitate further studies on the involvement of heat shock proteins in protective...
Tipo: Journal article
Palavras-chave: Piscirickettsia salmonis; Hsp60; Hsp70; GroEL; DnaK; Immune response.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602005000100009

Ethanol affects the absorption and tissue distribution of orally administered antigens in mice
Provedor de dados: 52
Autores: Oliveira,Flávia Márcia; Gusmão,Leandro José; Cardoso,Valbert do Nascimento; Ramaldes,Gilson Andrade; Ruiz-de-Souza,Valéria; Gontijo,Cristiano Machado.
The aim of this work was to evaluate the effects of ethanol on the adsorption and tissue distribution of orally administered antigens in mice. Results showed that ethanol reduced the level of anti-ovalbumin IgA antibodies in intestinal fluid for the mice treated orally with a palmitoyl-ovalbumin conjugate. Ethanol was administered intragastrically to mice at 5 g/kg body weight for 14 days (chronic treatment), or 10 g/kg body weight every 7th day up to 14 days (acute treatment). Thereafter, 99m technetium-labeled antigens were administered and lymphoid tissues were collected. Ethanol interfered with the transport of ovalbumin to the liver. Moreover, the transport of palmitoyl-ovalbumin to mesenteric lymph nodes was reduced 6 h after the antigen...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ethanol; Absorption; Biodistribution; Technetium; Immune response.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132013000400008

Genetic polymorphism and immune response to tuberculosis in indigenous populations: a brief review
Provedor de dados: 55
Autores: Longhi,Renata Maronna Praça; Zembrzuski,Verônica Marques; Basta,Paulo Cesar; Croda,Julio.
We systematically reviewed studies of the immune response to tuberculosis and the genetic polymorphisms associated with Th1-or Th2-mediated cytokine expression in indigenous populations. A bibliographic search was performed on the Medline and ISI databases and included studies published between January 1980 and October 2011. The search terms were tuberculosis, American Indians, Amerindian, indigenous, Indians, native people, aboriginal, immun*, host immune, immune response, cytokine*, polymorphism*, and gene. Regardless of their design, studies that evaluated immunoglobulin, cytokine levels and genetic polymorphisms that altered cytokine expression were included. Thirteen studies met the inclusion criteria. The majority of studies were performed in Latin...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Tuberculosis; Immune response; Indigenous populations; Polymorphism.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702013000300009

Humoral and cellular immune response of mice challenged with Yersinia pestis antigenic preparations
Provedor de dados: 55
Autores: Leal,Elida A.; Moreira,Josimar D.; Nunes,Fernanda F.; Souza,Larissa R.; Martins,Janaina M.; Toledo,Vicente P.C.; Almeida,Alzira M.P.; Guimarães,Tania M.P.
ABSTRACT  Objectives: The plague, which is an infectious disease caused by Yersinia pestis, still threatens many populations in several countries. The worldwide increase in human plague cases and the potential use of the bacteria as a biological weapon reinforce the need to study the immunity that is induced by potential vaccine candidates. To determine the immunogenicity of antigenic preparations based on the F1 protein and the total extract from Y. pestis, we assessed the role of these antigens in inducing an immune response.  Methods: The immunogenicity of antigenic preparations based on the Y. pestis (YP) total extract and the Y. pestis fraction 1 capsular antigen protein (F1) was determined in Swiss-Webster mice immunized with 40 µg or 20 µg for each...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Yersinia pestis; Antigens; Mice; Immune response; Vaccines.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702017000600620

Immune responses to Leptospira infection: roles as biomarkers for disease severity
Provedor de dados: 55
Autores: Chirathaworn,Chintana; Kongpan,Sutthikarn.
Various leptospiral components have been identified and shown to be involved in tissue destruction. In addition, immune responses to leptospires have been implicated in target organ damages in severe leptospirosis cases. Several inflammatory mediators were shown to be higher in susceptible animals than in resistant hosts. Moreover, cytokines/chemokines and serum proteins induced following Leptospira infection were suggested to be biomarkers for disease severity in human leptospirosis. This review focuses on the role of immune responses in the severity of leptospirosis. Studies in both animal models and humans are discussed.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Leptospirosis; Severity; Biomarker; Immune response.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702014000100077

Risk factors for poor immune response to influenza vaccination in elderly people
Provedor de dados: 55
Autores: Bellei,N.C.J.; Carraro,E.; Castelo,A.; Granato,C.F.H.
Influenza vaccination of elderly people is efficacious and cost effective for the prevention of influenza and its complications. Some studies have pointed out low immunogenicity in this group. Health status has been poorly investigated as a risk factor that may influence the immune response to influenza vaccine. We established an immunization response study of a highly-matched elderly population in a nursing home. One-hundred-twenty subjects of Ashkenazian origin had their vaccine-induced antibody response assessed. Good response was obtained in 30.8% (37/120), and 31.7% (38/120) did not react. A lack of good response was found to be associated with dementia (P=0.016) in a multivariate analysis. In addition to dementia, malnutrition was frequently observed...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Influenza; Vaccination; Immune response; Risk factors; Dementia.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702006000400011

Roles of monocyte chemotactic protein 1 and nuclear factor-κB in immune response to spinal tuberculosis in a New Zealand white rabbit model
Provedor de dados: 56
Autores: Guo,X.H.; Bai,Z.; Qiang,B.; Bu,F.H.; Zhao,N.
This study aimed to explore the roles of monocyte chemotactic protein 1 (MCP-1) and nuclear factor kappa B (NF-κB) in immune response to spinal tuberculosis in a New Zealand white rabbit model. Forty-eight New Zealand white rabbits were collected and divided into four groups: experimental group (n=30, spinal tuberculosis model was established), the sham group (n=15, sham operation was performed) and the blank group (n=3). The qRT-PCR assay and western blotting were applied to detect the mRNA and protein expressions of MCP-1 and NF-κB in peripheral blood. ELISA was used to measure serum levels of MCP-1, NF-κB, IFN-γ, IL-2, IL-4, and IL-10. Flow cytometry was adopted to assess the distributions of CD4+, CD8+ lymphocytes and CD4+ CD25+ Foxp3 lymphocyte...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Monocyte chemotactic protein 1; Nuclear factor-κB; Spinal tuberculosis; Immune response.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2017000300602

Induction of a gloverin-like antimicrobial polypeptide in the sugarcane borer Diatraea saccharalis challenged by septic injury
Provedor de dados: 56
Autores: Silva,J.L.C.; Barbosa,J.F.; Bravo,J.P.; Souza,E.M. de; Huergo,L.F.; Pedrosa,F.O.; Esteves,E.; Daffre,S.; Fernandez,M.A.
Diatraea saccharalis (Fabricius, 1794) (Lepidoptera: Crambidae) is an important pest for Brazilian sugarcane. In the present study, we detected two distinct spots in hemolymph from septic injured larvae (HDs1 and HDs2), which are separated by 2DE gel electrophoresis. Both spots were subjected to in-gel tryptic digestion and MALDI-TOF/TOF analysis, which revealed the sequence VFGTLGSDDSGLFGK present in both HDs1 and HDs2. This sequence had homology and 80% identity with specific Lepidoptera antimicrobial peptides called gloverins. Analyses using the ImageMaster 2D software showed pI 8.94 of the HDs1 spot, which is similar to that described to Hyalophora gloveri gloverin (pI 8.5). Moreover, the 14-kDa molecular mass of the spot HDs1 is compatible to that of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Diatraea saccharalis; Lepidoptera; Immune response; Gloverin; Sugarcane borer; Hemolymph.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500003

Immunological properties of gene vaccines delivered by different routes
Provedor de dados: 56
Autores: Oliveira,S.C.; Rosinha,G.M.S.; de-Brito,C.F.A.; Fonseca,C.T.; Afonso,R.R.; Costa,M.C.M.S.; Goes,A.M.; Rech,E.L.; Azevedo,V.
Gene vaccines represent a new and promising approach to control infectious diseases, inducing a protective immune response in the appropriate host. Several routes and methods of genetic immunization have been shown to induce antibody production as well as T helper (Th) cell and cytotoxic T lymphocyte activation. However, few studies have compared the nature of the immune responses generated by different gene vaccination delivery systems. In the present study we reviewed some aspects of immunity induced by gene immunization and compared the immune responses produced by intramuscular (im) DNA injection to gene gun-mediated DNA transfer into the skin of BALB/c mice. Using a reporter gene coding for ß-galactosidase, we have demonstrated that im injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Gene vaccination; Naked DNA; Gene gun; Cytokines; Immune response.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200009

Effect of TNF-α production inhibitors on the production of pro-inflammatory cytokines by peripheral blood mononuclear cells from HTLV-1-infected individuals
Provedor de dados: 56
Autores: Luna,T.; Santos,S.B.; Nascimento,M.; Porto,M.A.F.; Muniz,A.L.; Carvalho,E.M.; Jesus,A.R.
Human T lymphotropic virus type 1 (HTLV-1) is the causal agent of myelopathy/tropical spastic paraparesis (HAM/TSP), a disease mediated by the immune response. HTLV-1 induces a spontaneous proliferation and production of pro-inflammatory cytokines by T cells, and increasing interferon-γ (IFN-γ) and tumor necrosis factor-α (TNF-α) levels are potentially involved in tissue damage in diseases related to HTLV-1. This exaggerated immune response is also due to an inability of the natural regulatory mechanisms to down-modulate the immune response in this group of patients. TNF-α inhibitors reduce inflammation and have been shown to improve chronic inflammatory diseases in clinical trials. The aim of this study was to evaluate the ability of pentoxifylline,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HTLV-1; TNF-α inhibitors; Pentoxifylline; Immune response.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011001100009

Immunogenicity of a 23-valent pneumococcal polysaccharide vaccine in Brazilian elderly
Provedor de dados: 56
Autores: Simonsen,V.; Brandão,A.P.; Brandileone,M.C.C.; Yara,T.I.; Di Fabio,J.L.; Lopes,M.H.; Jacob Filho,W.
Serum antibodies specific for the capsular polysaccharides of Streptococcus pneumoniae provide protection against invasive pneumococcal infection. In Brazil, this vaccine has been used for people over 65 years with clinical risk to develop pneumococcal infection since 1999. We evaluated the immune response of 102 elderly subjects (75.5% females and 24.5% males) with a mean age of 71 years, and 19 young healthy adults (63.2% females and 36.8% males) with a mean age of 27 years. The elderly study group consisted of outpatients who received follow-up care in the Geriatric Department of General Hospital, Faculty of Medicine, University of São Paulo. None had acute illness at the time of vaccination. Both groups were immunized with one intra-deltoid injection...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Streptococcus pneumoniae; Vaccine; Pneumococcal polysaccharide vaccine; Immune response; Elderly subjects.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000200014

Antibody response in silver catfish (Rhamdia quelen) immunized with a model antigen associated with different adjuvants
Provedor de dados: 56
Autores: Pavan,T.R.; Di Domenico,J.; Kirsten,K.S.; Nied,C.O.; Frandoloso,R.; Kreutz,L.C.
Adjuvants are essential to boost the immune response to inoculated antigen and play a central role in vaccine development. In this study, we investigated the efficacy of several adjuvants in the production of anti-bovine serum albumin (BSA) antibodies in silver catfish. Two hundred and seventy juvenile silver catfish (60–80 g) of both sexes were intraperitoneally vaccinated with BSA (200 µg/fish) alone or mixed to the following adjuvants: Freund’s complete adjuvant (FCA), Freund’s incomplete adjuvant (FIA), aluminum hydroxide (AlOH), Montanide, four types of cytosine-phosphate-guanine (CpG) oligodeoxynucleotides (ODNs) and three concentrations of β-glucan, and the immune enhancing property was evaluated by measuring anti-BSA antibodies in blood samples at...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccination; Fish; Immune response; CpGs ODN; Β-glucan.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2016000800606

Main features of DNA-based immunization vectors
Provedor de dados: 56
Autores: Azevedo,V.; Levitus,G.; Miyoshi,A.; Cândido,A.L.; Goes,A.M.; Oliveira,S.C.
DNA-based immunization has initiated a new era of vaccine research. One of the main goals of gene vaccine development is the control of the levels of expression in vivo for efficient immunization. Modifying the vector to modulate expression or immunogenicity is of critical importance for the improvement of DNA vaccines. The most frequently used vectors for genetic immunization are plasmids. In this article, we review some of the main elements relevant to their design such as strong promoter/enhancer region, introns, genes encoding antigens of interest from the pathogen (how to choose and modify them), polyadenylation termination sequence, origin of replication for plasmid production in Escherichia coli, antibiotic resistance gene as selectable marker,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genetic immunization; DNA plasmid; Enhancer/promoter region; Immunostimulatory sequences (ISS); Antigens; Immune response.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000200002

Hormone replacement therapy increases levels of antibodies against heat shock protein 65 and certain species of oxidized low density lipoprotein
Provedor de dados: 56
Autores: Uint,L.; Gebara,O.C.E.; Pinto,L.B.; Wajngarten,M.; Boschcov,P.; Luz,P.L. da; Gidlund,M.
Hormone replacement therapy (HRT) reduces cardiovascular risks, although the initiation of therapy may be associated with transient adverse ischemic and thrombotic events. Antibodies against heat shock protein (Hsp) and oxidized low density lipoprotein (LDL) have been found in atherosclerotic lesions and plasma of patients with coronary artery disease and may play an important role in the pathogenesis of atherosclerosis. The aim of the present study was to assess the effects of HRT on the immune response by measuring plasma levels of antibodies against Hsp 65 and LDL with a low and high degree of copper-mediated oxidative modification of 20 postmenopausal women before and 90 days after receiving orally 0.625 mg equine conjugate estrogen plus 2.5 mg...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Hormone replacement therapy; Postmenopause; Oxidized LDL; Heat shock protein; Immune response; Antibodies.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2003000400011

Association among genetic predisposition, gut microbiota, and host immune response in the etiopathogenesis of inflammatory bowel disease
Provedor de dados: 56
Autores: Basso,P.J.; Fonseca,M.T.C.; Bonfá,G.; Alves,V.B.F.; Sales-Campos,H.; Nardini,V.; Cardoso,C.R.B.
Inflammatory bowel disease (IBD), which includes Crohn's disease (CD) and ulcerative colitis (UC), is a chronic disorder that affects thousands of people around the world. These diseases are characterized by exacerbated uncontrolled intestinal inflammation that leads to poor quality of life in affected patients. Although the exact cause of IBD still remains unknown, compelling evidence suggests that the interplay among immune deregulation, environmental factors, and genetic polymorphisms contributes to the multifactorial nature of the disease. Therefore, in this review we present classical and novel findings regarding IBD etiopathogenesis. Considering the genetic causes of the diseases, alterations in about 100 genes or allelic variants, most of them in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Inflammatory bowel disease; Crohn's disease; Ulcerative colitis; Immune response; Etiology; Microbiota.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2014000900727

Evaluation of immunomodulatory effects of lomefloxacin in mice
Provedor de dados: 60
Autores: Majeed,Arfa; Javeed,Aqeel; Omer,Muhammad Ovais; Mushtaq,Muhammad Hassan; Sattar,Adeel.
Lomefloxacin is a flouroquinolone antibiotic that is quite efficacious against many gram negative and gram positive pathogens. Lomefloxacin evince antibacterial effects by modifying DNA gyrase in gram negative pathogens and topoisomerase IV in gram positive pathogens. This study is designed to assess the immunomodulatory effects of lomefloxacin in male albino mice. Three doses of lomefloxacin 12.5 mg/kg, 25 mg/kg and 50 mg/kg were used and delayed type hypersensitivity assay, cyclophosphamide induced neutropenic assay, carbon clearance assay, heamagglutination assay and mice lethality test were performed to evaluate the effects of lomefloxacin on immune system of mice. DTH assay has depicted the significant immunosuppressant potential of lomefloxacin at 25...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cell-mediated; Humoral; Immunomodulatory; Immune response; Lomefloxacin.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100628