Consulta: subjectFacets:"Immunization"
Registros recuperados: 27
Data/hora: 10/06/2024 06:24:15

PRODUCTION OF AN EFFECTIVE ANTI-Bothrops-TETANUS MIXED HYPERIMMUNE SERUM OF EQUINE ORIGIN
Provedor de dados: 79
Autores: GUIDOLIN,R.; STEPHANO,M. A.; MORAIS,J. F.; SAKAGUTI,E. H.; PRADO,S. M. A.; FRATELLI,F.; VANCETTO,M. D. C.; HIGASHI,H. G.
The present investigation reveals the possibility of simultaneous immunization of horses with Bothrops or Crotalus snake venoms and Tetanus antigens for the production of anti-Bothrops-Tetanus or anti-Crotalus-Tetanus mixed serum, with high titers of the respective specific antibodies. Bothrops antivenoms with an average neutralizing titer of 4.16 mg venom/ml were obtained from plasma of horses with titers lower than 0.5 mg venom/ml when Tetanus antigens were not used. This suggests the existence of a synergism between Bothrops venoms and Tetanus antigens in the stimulation of the antibody response. The pooled plasma of the animals had a neutralizing titer of 21.0 mg/ml reference Bothrops venoms and 3,300 IU/ml to Tetanus antigens after purification by...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antivenoms; Immunization; Bothrops; Tetanus.
Ano: 1998
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0104-79301998000100007

Destruction of immunobiologicals and their disposal
Provedor de dados: 80
Autores: Gatti,MAN; Cardoso,SNM; Simeão,SFAP; Vitta,A; Monteiro,LM.
The guidelines of the Brazilian National Health System (SUS) state that the municipal authorities are responsible for the vaccination of the population. The present study examined the types of immunobiologicals, amounts and reasons for their destruction and disposal by the Municipal Health Secretariat in the city of Bauru, São Paulo state, Brazil, between 2008 and 2009. This study comprises a descriptive, exploratory and retrospective work that employed search of bibliographic data, collection of secondary data from forms of immunobiologicals disposal and interview of the agent responsible for the disposal of these products in the municipality. It was observed that the total numbers of unused vaccines in 2008 and 2009 were similar (4523 and 4395,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Biologic waste disposal; Health services.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000400017

Adjuvant Effect of Oligomannose-Coated Liposome-Based Platform for veccine against African Trypanosomosis
Provedor de dados: 19
Autores: Kuboki, Noritaka; Yokoyama, Naoaki; Namangala, B; Okamura, Masashi; Inoue, Noboru; Takagi, Hideaki; Nakayama, Tomoko; Nishikawa, Yoshifumi; Ikehara, Yuzuru; Kojima, Naoya; 横山, 直明; 井上, 昇; 西川, 義文.
Palavras-chave: Adjuvant; African trypanosome; Immunization; Oligomannose-coated liposome(OML).
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2222

Active Immunization Against 5α-androstane-3α, 17β-diol Increases Ovulation Rate in Merino Ewes
Provedor de dados: 19
Autores: TETSUKA, Masafumi; NANCARROW, Colin. D.; 手塚, 雅文.
Cyclic Merino ewes were immunized three times against 5α−androstane-3α,17β-diol (3α-diol) at three week intervals (weeks 0,3 and 6). After the third immunization, estrous cycles were synchronized by a series of PG injections and intensive blood samples were obtained for gonadotropin analyses during the mid-luteal phase and during the early follicular phase. Ovulation rates were determined after each immunization and subsequent cloprostenol (PG)-induced luteolysis (weeks 3, 6, 8 and 11). After the second immunization, daily blood samples were obtained and concentrations of plasma progesterone (P) were measured. Immunization against 3α-diol significantly increased the ovulation rate after the second and third immunizations and at week 10 (P&lt;0.01,...
Palavras-chave: Ovulation rate; Sheep; Immunization; 5a-reduced androgen.
Ano: 2001
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3033

Peroxiredoxin 3 promotes IL-12 production from macrophages and partially protects mice against infection with Toxoplasma gondii
Provedor de dados: 19
Autores: Fereig, Ragab M.; Nishikawa, Yoshifumi.
Toxoplasmosis remains a life-threatening infection of humans and various domestic and wild animals worldwide. It is caused by the obligatory intracellular protozoan parasite Toxoplasma gondii. Peroxiredoxins (Prxs) are a family of antioxidant enzymes that protect cells from oxidative stress from hydroperoxides. In the recent years, several studies have reported the potential use of T. gondii-derived enzymes in triggering protective immunity against T. gondii infection. Therefore, this study was conducted to investigate the immunogenicity and protective efficacy of TgPrx3. In vitro stimulation of peritoneal macrophages with recombinant TgPrx3 protein fused to glutathione-S transferase (TgPrx3-GST) enhanced IL-12p40 production, indicating the...
Palavras-chave: Toxoplasma gondii; Peroxiredoxin 3; Vaccine; Immunization.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4569

Alternatives for methane emission mitigation in livestock systems
Provedor de dados: 96
Autores: Lascano,Carlos E.; Cárdenas,Edgar.
Human activities are contributing to Global Climate Change through the production of Green House Gases (GHG), which result in increased air, land and ocean temperatures and extreme changes in precipitation in regions of low and high rainfall. The most important GHG's are carbon dioxide (CO2), methane (CH4) and nitrous oxide (N2O). It is estimated that 18 % of the annual GHG emissions come from different types of livestock and that 37% of CH4, with higher global warming potential (23) relative to CO2 (1), comes from fermentation processes in ruminants. It is possible that in the future beef and milk exports from producing countries is subject to bans if cattle systems do not comply with measures to reduce GHG. There are several alternatives available and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Climate change; Defaunation; Feed chemical additives; Genetic transformation of bacteria; Greenhouse gases; Immunization; Saponins; Tannins.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982010001300020

Low cost production and purification of polyclonal antibodies to staphylococcal enterotoxin A
Provedor de dados: 59
Autores: Oliveira,Tereza Cristina R.M. de; Hirooka,Elisa Yoko.
An immunization scheme for production of antiserum to staphylococcal enterotoxin A (SEA) is proposed. The reference method of Robbins and Bergdoll was modified to reduce the number of doses and the amount of toxin used per animal. The best immunization scheme used injections in days 0, 8, 24, 59, 62 and 67. The amount of toxin at each injection was 5, 6, 20, 50, 100 and 200&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, respectively. The total amount of toxin was 381&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g, which corresponded to a reduction of 107&lt;FONT FACE="Symbol"&gt;m&lt;/font&gt;g in the amount of toxin for each animal when compared to the reference method. The average antiserum titer using the Optimum Sensitivity Plate - OSP was 1:60 and using ELISA...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Staphylococcus aureus; Enterotoxins; Detection; Immunization.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000200006

Migration of Schistosoma mansoni sambon (Trematoda, Schistosomatidae) from skin to lungs in immunized NZ rabbits (Lagomorpha, Leporidae) by autoradiographic analysis
Provedor de dados: 104
Autores: Pinto,R. Magalhães; Noronha,Dely; Almeida,Marília S. S; Katz,Naftale; Tendler,Miriam.
Skin to lung migration of Schistosoma mansoni Sambon, 1907 - (75 Se) - selenomethionine-labeled cercariae was tracked by tissue autoradiography on days 1, 4, 6, 8 after challenge, in rabbits immunized with a S. mansoni derived saline extract. Either in vaccinated animals or in those of the control unprimed group, the peak of skin schistosomula occurs 24hs after infection. Comparison between peaks of lungs migrating larvae showed that, in control animals, the increase of worm burden in this site, is detected on the 6th day post-infection, differing from immunized rabbits, in which this peak occurs on day 4, when skin and lungs counts are still equivalent, decreasing gradually, showing a different pattern of the S. mansoni migration and suggesting that main...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Schistosoma mansoni; Migration; Autoradiography; Immunization; Rabbits.
Ano: 1994
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81751994000400006

Bacillus subtilis as a tool for vaccine development: from antigen factories to delivery vectors
Provedor de dados: 42
Autores: Ferreira,Luís C.S.; Ferreira,Rita C.C.; Schumann,Wolfgang.
Bacillus subtilis and some of its close relatives have a long history of industrial and biotechnological applications. Search for antigen expression systems based on recombinant B. subtilis strains sounds attractive both by the extensive genetic knowledge and the lack of an outer membrane, which simplify the secretion and purification of heterologous proteins. More recently, genetically modified B. subtilis spores have been described as indestructible delivery vehicles for vaccine antigens. Nonetheless both production and delivery of antigens by B. subtilis strains face some inherent obstacles, as unstable gene expression and reduced immunogenicity that, otherwise, can be overcome by already available gene technology approaches. In the present review we...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Vaccines; Bacillus subtilis; Immunization; Protein expression.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652005000100009

Efficiency of inactive vaccines against contagious agalactia in Brazil
Provedor de dados: 45
Autores: Campos,A.C.; Azevedo,E.O.; Alcântara,M.D.B.; Silva,R.B.S.; Cordeiro,A.A.; Mamede,A.G.; Melo,M.A.; Rosendo Nascimento,E.; Castro,R.S.
This paper aims to evaluate the efficiency of three inactive vaccines against contagious agalactia prepared with samples of Mycoplasma agalactiae isolated in Brazil and different adjuvants. Vaccine 1 adsorbed with aluminum hydroxide was administered in 23 goats (Gc1) and 13 sheep (Gov1); vaccine 2 containing Montanide IMS-2215-VG was administered in 22 goats (Gc2) and 12 sheep (Gov2) and vaccine 3, containing Montanide Gel-01 was administered in 22 goats (Gc3) and 12 sheep (Gov3). All animals were negative for Ma at indirect ELISA and received two doses of 2mL each, subcutaneously, within a 21 day interval. Five animals from each species were used as control. Seventy-five days after the booster, four animals from each vaccinated group and two from the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Mycoplasma agalactiae; Goats; Sheep; Immunization; ELISA.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352013000500018

Assessment of the acid phosphatase CP01850 from Corynebacterium pseudotuberculosis in DNA and subunit vaccine formulations against caseous lymphadenitis
Provedor de dados: 45
Autores: Rezende,A.F.S.; Brum,A.A.; Bezerra,F.S.B.; Braite,D.C.; Sá,G.L.; Thurow,H.S.; Seixas,F.K.; Azevedo,V.A.C.; Portela,R.W.; Borsuk,S.
ABSTRACT The target cp1002_RS01850 from Corynebacterium pseudotuberculosis was used to construct a DNA and recombinant subunit vaccine against caseous lymphadenitis. Recombinant protein rCP01850 was expressed in Escherichia coli using pAE vector, and DNA vaccine was engineered with pTARGET vector. BALB/c mice were divided in five groups containing eight animals each, inoculated with: pTARGET/cp01850 as DNA vaccine (G1); rCP01850 plus Al (OH)3 as recombinant subunit vaccine (G2); pTARGET/cp01850 and a boost with rCP01850 plus Al (OH)3 (G3); pTARGET (G4); or Al (OH)3 (G5). Mice were inoculated and blood samples were collected on days 0, 21, and 42 for the analysis of total IgG, IgG1 and IgG2a by ELISA. In each group, five animals were challenged with Mic-6...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cp1002_RS01850 gene; Immunization; Recombinant protein; Aluminum hydroxide; Heterologous prime-boost strategy.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352020000100199

Assessment of the plan for pre-exposition vaccination with Fuenzalida-Palacios anti-rabies vaccine
Provedor de dados: 52
Autores: Preto,Angela Aparecida; Gomes,Elisa Maines; Fernandes,Maurício José; Hennig,Nei Adolfo; Germano,Pedro Manoel Leal.
Studies ere carried out to monitor the titres of anti-rabies antibodies taken from individuals exposed to the risk of infection. Fifty-seven individuals aged between 20 and 40, were vaccinated with a minimum titre of 1.3 IU Fuenzalida-Palacios type anti-rabies vaccine. The vaccination plan consisted of the application of three doses at two day intervals and a booster applied 28 days after the final dose. The accompainment of the antibody titres was achieved by means of the seroneutralization test over a total period of 18 months. Through the monitoring carried out, it was found to be necessary to apply a booster vaccination to part of the group. Some of these individuals were revaccinated with Fuenzalida-Palacios type vaccine with a minimum titre of 1.3...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Rabies vaccine; Antibodies; Neutralization; Fuenzalida-Palacios.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132000000300002

Vaccination status of people living with HIV/AIDS in outpatient care in Fortaleza, Ceará, Brazil
Provedor de dados: 55
Autores: Cunha,Gilmara Holanda da; Galvão,Marli Teresinha Gimeniz; Medeiros,Camila Martins de; Rocha,Ryvanne Paulino; Lima,Maria Amanda Correia; Fechine,Francisco Vagnaldo.
Abstract Antiretroviral therapy has increased the survival of patients with HIV/AIDS, thus necessitating health promotion practice with immunization. Vaccines are critical components for protecting people living with HIV/AIDS (PLWHA). The purpose of study was to analyze the vaccination status of PLWHA in outpatient care in Fortaleza, Ceará, Brazil. Cross-sectional study performed from June 2014 to June 2015. The screening was done with patients in antiretroviral therapy, 420 patients underwent screening, but only 99 met the inclusion criteria. Data were collected for interviews using forms to characterize sociodemographic, clinical and vaccination situations. Only 14 patients had complete vaccination schedules. The most used vaccines were hepatitis B,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Acquired immunodeficiency syndrome; HIV; Health promotion; Immunization; Immunization schedule.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702016000500487

A 19-year old man with IgA vasculitis after vaccination
Provedor de dados: 55
Autores: Malek,Alexandre; Gomez-Villegas,Sara Isabel; de la Hoz,Alejandro; Nowbakht,Cima; Arias,Cesar A.
ABSTRACT A 19-year-old patient who mistakenly received two doses of influenza vaccine 10 days before presentation, was admitted with malaise, weakness, and a purpuric non-blanching rash most prominent on the ankles followed by abdominal pain and hematochezia 72 h later. The diagnosis of influenza vaccine-related Henoch–Schonlein vasculitis was made. This complication, although rare, is the most common vasculitis related to immunization.
Tipo: Info:eu-repo/semantics/report
Palavras-chave: Influenza vaccination; Immunization; IgA vasculitis; Henoch–Schonlein purpura.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000500442

Individual variation is the key to the development of a vaccine against Staphylococcus aureus: a comparative study between mice lineages
Provedor de dados: 56
Autores: dos Santos,D.P.; Muniz,I.P.R.; Queiroz,A.F.; Pereira,I.S.; Souza,M.P.A.; Lima,L.J.; Sousa,L.R.O.; Ribeiro,I.S.; Galantini,M.P.L.; Marques,L.M.; Figueiredo,T.B.; da Silva,R.A.A.
Bacterial infections occur worldwide and are a major public health problem. Among pathogens, Staphylococcus aureus is the main causative agent of bacterial diseases in the world. This study aimed to evaluate which components of the immune system could act protectively against a S. aureus infection in intradermally immunized mice. C57BL/6 and A/j mice were immunized intradermally with S. aureus inactivated by heat and then challenged with viable strains in an air pouch model. At 6, 12, and 24 h after the challenge, euthanasia was performed, and the cellular profile of the inflammatory infiltrate, cytokines, and the bacterial load were evaluated in the air pouch lavages. Immunized mice demonstrated that the intradermal immunization with S. aureus promoted...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Immunization; Staphylococcus aureus; Mice; Air pouch; Antibodies.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2018000500616

Coping with genetic diversity: the contribution of pathogen and human genomics to modern vaccinology
Provedor de dados: 56
Autores: Lemaire,D.; Barbosa,T.; Rihet,P.
Vaccine development faces major difficulties partly because of genetic variation in both infectious organisms and humans. This causes antigenic variation in infectious agents and a high interindividual variability in the human response to the vaccine. The exponential growth of genome sequence information has induced a shift from conventional culture-based to genome-based vaccinology, and allows the tackling of challenges in vaccine development due to pathogen genetic variability. Additionally, recent advances in immunogenetics and genomics should help in the understanding of the influence of genetic factors on the interindividual and interpopulation variations in immune responses to vaccines, and could be useful for developing new vaccine strategies....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Human vaccines; Vaccinomics; Genetic variation; Immunization.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2012000500001

Prevalence of diphtheria and tetanus antibodies and circulation of Corynebacterium diphtheriae in São Paulo, Brazil
Provedor de dados: 56
Autores: Divino-Goes,K.G.; Moraes-Pinto,M.I. de; Dinelli,M.I.S.; Casagrande,S.T.; Bonetti,T.C.S.; Andrade,P.R.; Weckx,L.Y.
The introduction of routine vaccination against tetanus and diphtheria in Brazil has decreased the incidence and changed the epidemiology of both diseases. We then investigated the prevalence of Corynebacterium diphtheriae carrier status and diphtheria and tetanus immunity in São Paulo, Brazil. From November 2001 to March 2003, 374 individuals were tested for the presence of C. diphtheriae in the naso-oropharynx and of serum diphtheria and tetanus antibodies. Participants were all healthy individuals without acute or chronic pathologies and they were stratified by age as follows: 0-12 months and 1-4, 5-9, 10-14, 15-24, 25-39, 40-59, and ³60 years. Antibodies were assessed using a double-antigen ELISA. C. diphtheriae species were identified by biochemical...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Diphtheria; Tetanus; Corynebacterium diphtheriae; Vaccine; Immunization; Antibody.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007001200012

Enhanced inhibition of murine prostatic carcinoma growth by immunization with or administration of viable human umbilical vein endothelial cells and CRM197
Provedor de dados: 56
Autores: Huiyong,Zhang; Yong,Lu; Didier,Mekoo; Yu,Zhang; Jing,Fang; Rongyue,Cao; Jingjing,Liu.
Vaccination with xenogeneic and syngeneic endothelial cells is effective for inhibiting tumor growth. Nontoxic diphtheria toxin (CRM197), as an immunogen or as a speciﬁc inhibitor of heparin-binding EGF-like growth factor, has shown promising antitumor activity. Therefore, immunization with or administration of viable human umbilical vein endothelial cells (HUVECs) combined with CRM197 could have an enhanced antitumor effect. Six-week-old C57BL/6J male mice were vaccinated with viable HUVECs, 1 x 10(6) viable HUVECs combined with 100 μg CRM197, or 100 μg CRM197 alone by ip injections once a week for 4 consecutive weeks. RM-1 cells (5 x 10(5)) were inoculated by sc injection as a preventive procedure. During the therapeutic procedure, 6-week-old male...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: RM-1 prostatic carcinoma; CRM197; Immunization; Human umbilical vein endothelial cells.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2011000200008

Effect of a single tetanus-diphtheria vaccine dose on the immunity of elderly people in São Paulo, Brazil
Provedor de dados: 56
Autores: Weckx,L.Y.; Divino-Goes,K.; Lihama,D.M.; Carraro,E.; Bellei,N.; Granato,C.F.H.; de Moraes-Pinto,M.I.
Epidemiological data regarding tetanus and diphtheria immunity in elderly people in Brazil are scarce. During the First National Immunization Campaign for the Elderly in Brazil in April 1999, 98 individuals (median age: 84 years) received one tetanus-dyphtheria (Td) vaccine dose (Butantan Institute, lot number 9808079/G). Inclusion criteria were elderly individuals without a history of severe immunosuppressive disease, acute infectious disease or use of immunomodulators. Blood samples were collected immediately before the vaccine and 30 days later. Serum was separated and stored at -20ºC until analysis. Tetanus and diphtheria antibodies were measured by the double-antigen ELISA test. Tetanus and diphtheria antibody concentrations lower than 0.01 IU/mL were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Elderly; Tetanus; Diphtheria; Immunization.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2006000400012

Occurrence of Haemophilus influenzae strains in three Brazilian states since the introduction of a conjugate Haemophilus influenzae type b vaccine
Provedor de dados: 56
Autores: de Almeida,A.E.C.C.; de Filippis,I.; Abreu,A.O. de; Ferreira,D.G.; Gemal,A.L.; Marzochi,K.B.F.
Few vaccines in history have induced such a dramatic decline in incidence over such a short period of time as the Haemophilus influenzae type b (Hib) conjugate. This vaccine was introduced in 1988 in the United States, but only in 1999 was Hib immunization introduced by the Brazilian Ministry of Health as part of the routine infant National Immunization Program. The authors analyzed 229 H. influenzae (Hi) isolates from Public Health Laboratories in three Brazilian states: Pernambuco (Northeast, N = 54), Santa Catarina (South, N = 19), and Rio de Janeiro (Southeast, N = 156). The isolates were collected from Brazilian children 0-10 years of age with meningitis and other infections from 1990 to 2003 and were part of the research collection of the National...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Haemophilus influenzae; Haemophilus influenzae non-b; Hib conjugate vaccine; Immunization; Hib meningitis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2005000500016

Induction of neutralizing antibodies in mice immunized with scorpion toxins detoxified by liposomal entrapment
Provedor de dados: 56
The possibility of producing neutralizing antibodies against the lethal effects of scorpion toxins was evaluated in the mouse model by immunization with an immunogen devoid of toxicity. A toxic fraction (5 mg) from the venom of the scorpion Tityus serrulatus was entrapped in sphingomyelin-cholesterol liposomes. The liposomes were treated for 1 h at 37oC with a 1% (w/w) trypsin solution in 0.2 M sodium carbonate buffer, pH 8.3. This treatment led to a strong reduction in venom toxicity. Immunization was performed as follows: mice were injected sc with 20 µg of the liposome-entrapped toxic fraction on days 1 and 21 and a final injection (20 µg) was administered ip on day 36. After injection of the immunogen, all mice developed an IgG response which was shown...
Palavras-chave: Liposomes; Neutralizing antibodies; Tityus serrulatus; Immunization; Toxins.
Ano: 1997
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1997000700010

The contribution of Latin American research to HPV epidemiology and natural history knowledge
Provedor de dados: 56
Autores: Sichero,L.; Picconi,M.A.; Villa,L.L.
Our aim was to review the major contributions of studies conducted in different Latin American (LA) countries to the field of human papillomavirus (HPV) epidemiology, natural history, risk of disease, and prevention strategies, mainly in the uterine cervix. Although cytological screening is established in several countries in LA, incidence and mortality rates from cervical cancer (CC) are still extremely high. Finally, data from large cohort studies conducted in LA countries provided seminal data to propose primary and secondary prevention modalities: the HPV vaccine has been introduced in the national immunization programs of several LA countries and multiple screening experiences using HPV testing are under evaluation in the region.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: HPV; Latin America; Immunization; Cervical cancer; Natural history; Screening.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2020000200302

A glycoprotein E gene-deleted bovine herpesvirus 1 as a candidate vaccine strain
Provedor de dados: 56
Autores: Weiss,M.; Brum,M.C.S.; Anziliero,D.; Weiblen,R.; Flores,E.F.
A bovine herpesvirus 1 (BoHV-1) defective in glycoprotein E (gE) was constructed from a Brazilian genital BoHV-1 isolate, by replacing the full gE coding region with the green fluorescent protein (GFP) gene for selection. Upon co-transfection of MDBK cells with genomic viral DNA plus the GFP-bearing gE-deletion plasmid, three fluorescent recombinant clones were obtained out of approximately 5000 viral plaques. Deletion of the gE gene and the presence of the GFP marker in the genome of recombinant viruses were confirmed by PCR. Despite forming smaller plaques, the BoHV-1△gE recombinants replicated in MDBK cells with similar kinetics and to similar titers to that of the parental virus (SV56/90), demonstrating that the gE deletion had no deleterious effects...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle pathogen; Immunization; Control; Differential vaccine.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2015000900843

Immunization by subcutaneous implants of polyester-polyurethane sponges coupled with antigen
Provedor de dados: 56
Autores: Lima,S.L.; Machado,C.B.; Pereira,M.A.C.; Cara,D.C.; Velarde,D.T.; Andrade,S.P.; Gontijo,C.M.
A new protocol is described for immunization of outbred Swiss mice. The procedure is based on subcutaneous implantation of antigen-coupled polyester-polyurethane sponges cut into disks of 10 mm in diameter vs 2 mm in thickness. Antigen coupling was performed by overnight incubation of the sponge with a solution of ovalbumin (Ova) (2 mg/ml) diluted in sodium carbonate buffer, pH 9.6. The amount of ovalbumin that was taken up by the sponge was between 71.4 to 82.5 µg. This was estimated by comparing the Ova absorbance at 280 nm in coating buffer solutions before and after incubation. To compare the efficiency of the proposed method, experimental groups immunized with the antigen in the presence of adjuvants (10 µg in Al(OH)3 or 100 µg in complete Freund's...
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Sponge; Implant; Immunization; Vaccine.
Ano: 1999
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X1999000400011

Tetanus and diphtheria immunity in adolescents from São Paulo, Brazil
Provedor de dados: 56
Autores: Dinelli,M.I.S; Fisberg,M; Moraes-Pinto,M.I de.
Tetanus and diphtheria vaccines are of special concern in adolescents because boosters are necessary for adequate maintenance of protection and are often omitted. We assessed serum levels of tetanus and diphtheria antibodies in adolescents and their association with vaccination status. From May to October 2001, we evaluated the vaccination records of 208 adolescents aged 10 to 20 years in São Paulo, Brazil. Antibodies to tetanus and diphtheria were detected using double-antigen ELISA and vaccination records were analyzed according to the guidelines of the Brazilian National Immunization Program. All adolescents had received complete primary vaccinations against tetanus and diphtheria, but 23.1% of them had not received a booster dose in the last 10 years....
Tipo: Info:eu-repo/semantics/other
Palavras-chave: Vaccine; Tetanus; Diphtheria; Adolescents; Antitoxins; Immunization.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000200013

Diagnostics of tuberculosis and differentiation of nonspecific tuberculin reactions in animals
Provedor de dados: 58
Autores: Basybekov,Sovetzhan Z.; Bazarbayev,Marat B.; Yespembetov,Bolat A.; Mussaeva,Assiya; Kanatbayev,Serik G.; Romashev,Kanapya M.; Dossanova,Aigul K.; Yelekeyev,Tokseiit A.; Akmatova,Elmira K.; Syrym,Nazym S.
Abstract Tuberculosis is a serious disease of humans and animals, caused by bacteria of the Mycobacterium genus. This leads to complications in the life of the sick person, and subsequently to death. The cattle, who have been diagnosed with this bacterium, are usually sent to the slaughter, with the result that their livestock is reduced. Mycobacteriosis is also a disease, after determining which cattle are most often sent to slaughter. Such a reduction in livestock numbers has a negative effect on the economy. Of the 300 samples from the animals, 25 cultures of atypical bacteria responding to tuberculin were isolated. A series of tests - intravenous tuberculin test, ophthalmic test, palpebral test, "ZhAT" test, showed that most of the tuberculosis changes...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattle; Atypical mycobacterium; Tuberculin skin test; Palpebral method; Immunization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200329

Reproductive performance of Nellore heifers raised in extensive system undergoing different vaccination protocols in fixed-time artificial insemination (FTAI)
Provedor de dados: 65
Autores: Schillings Neto,Ernest; Nogueira,Eriklis; Jardim,Rodrigo; Abreu,Urbano Gomes Pinto de; Sterza,Fabiana Andrade de Melo; Pellegrin,Aiesca Oliveira; Juliano,Raquel Soares.
ABSTRACT:  This study evaluated the reproductive performance of Nellore heifers without previous vaccination undergoing fixed-time artificial insemination (FTAI) and subsequent natural breeding under different vaccination protocols against infectious bovine herpesvirus 1 (BHV-1), bovine viral diarrhea virus (BVDV), and bovine leptospirosis (BL). A total of 763 animals were included; the group assignment was as follows: T1, control without vaccination; T2, vaccination against BL; T3, vaccination against BHV-1and BVDV; T4,vaccination against combination of BL + BHV-1and BVDV. Vaccination was started 30 days before FTAI (D-40), and a booster dose was administered at D-10. Serological tests were used to verify the occurrence rate of natural infection by BL,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Beef cattle; Immunization; Leptospirosis; Pregnancy rate; Viral diseases.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019001100701