Consulta: subjectFacets:"In vitro culture"
Registros recuperados: 66
Data/hora: 03/06/2024 02:09:28

Propagación in vitro de selecciones de guayabo (Psidium guajava L.) y su respuesta a hormonas y periodos de subcultivo.
Provedor de dados: 32
Autores: Domínguez Perales, Luis Antonio.
La obtención de plantas de guayabo a partir de métodos de propagación como: hijuelos de raíz o por semilla, afecta sus rendimientos y su sanidad, considerando también que la propagación a partir de semillas genera una gran variación en la forma, tamaño y calidad de los frutos. La regeneración in vitro de plantas completas de guayabo puede ser la alternativa o complemento perfecto de los métodos convencionales de propagación, al obtener plantas a gran escala, uniformes y libres de microorganismos. En esta investigación se desarrollaron protocolos para la propagación in vitro de dos selecciones de Psidium guajava L. A partir de brotes obtenidos in vitro se consiguió establecer una metodología para su multiplicación en medio basal MS adicionado con sacarosa...
Palavras-chave: Psidium guajava L.; Micropropagación; Organogénesis directa; In vitro culture; Shoot regeneration; Genética; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/518

Propagación in vitro de selecciones de guayabo (Psidium guajava L.) y su respuesta a hormonas y periodos de subcultivo.
Provedor de dados: 32
Autores: Domínguez Perales, Luis Antonio.
La obtención de plantas de guayabo a partir de métodos de propagación como: hijuelos de raíz o por semilla, afecta sus rendimientos y su sanidad, considerando también que la propagación a partir de semillas genera una gran variación en la forma, tamaño y calidad de los frutos. La regeneración in vitro de plantas completas de guayabo puede ser la alternativa o complemento perfecto de los métodos convencionales de propagación, al obtener plantas a gran escala, uniformes y libres de microorganismos. En esta investigación se desarrollaron protocolos para la propagación in vitro de dos selecciones de Psidium guajava L. A partir de brotes obtenidos in vitro se consiguió establecer una metodología para su multiplicación en medio basal MS adicionado con sacarosa...
Palavras-chave: Psidium guajava L.; Micropropagación; Organogénesis directa; In vitro culture; Shoot regeneration; Genética; Maestría.
Ano: 2011
URL: http://hdl.handle.net/10521/518

Immature embryo: A useful tool for oil palm (Elaeis guineensis Jacq.) genetic transformation studies
Provedor de dados: 69
Autores: Abdullah,Ruslan; Zainal,Alizah; Yew Heng,Wee; Chui Li,Leaw; Chee Beng,Yeap; Mei Phing,Lee; Abdullah Sirajuddin,Salwa; Soo Ping,Winnie Yap; Lourdes Joseph,Juanita; Azma Jusoh,Siti.
Oil palm (Elaeis guineensis Jacq.) is the highest yielding oil-bearing crop. However, being a perennial crop, genetic improvement of oil palm is extremely slow. Indeed, compared to other annual oil crops such as soybean and rapeseed, genetic manipulations remained less important. Therefore, to remain competitive, oil palm growers and breeders need new and novel approaches. In this report, the potential of immature embryos (IE) as a useful tool for oil palm genetic transformation studies was evaluated. It was evident that IEs were amenable to both direct and Agrobacterium-mediated gene transfer. Due to the abundant supply of IE, optimization of biolistic and Agrobacterium-mediated gene transfer into IEs were easily carried out. Transient transformation...
Tipo: Journal article
Palavras-chave: Genetic transformation studies; Immature embryos; In vitro culture; Oil palm; Plant regeneration.
Ano: 2005
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582005000100006

In vitro plant regeneration system for common bean (Phaseolus vulgaris): effect of N6-benzylaminopurine and adenine sulphate
Provedor de dados: 69
Autores: Gatica Arias,Andrés M; Muñoz Valverde,Jenny; Ramírez Fonseca,Pilar; Valdez Melara,Marta.
A method for regeneration of the commercially important common bean (Phaseolus vulgaris ) using N6-benzylaminopurine(BAP) and adenine sulphate (AS) was established. Embryogenic axes of the Costa Rican common bean cultivars Bribrí, Brunca, Guaymí, Huetar and Telire were cultured on Murashige and Skoog medium supplemented with 100 mgl-1 myo-inositol, 1 mgl-1 thiamine, 30 gl-1 sucrose, BAP (0, 5 and 10 mgl-1), AS (0, 20 and 40 mgl-1) and 8 gl-1 agar. Regardless of the concentration of BAP and AS in the induction medium, the number of shoots and leaves differed significantly among the common bean cultivars evaluated. The higher average of shoots was obtained for Brunca &gt; Telire &gt; Bribrí &gt; Guaymí &gt; Huetar. Moreover, independently of the cultivar,...
Tipo: Journal article
Palavras-chave: Common bean; In vitro culture; Morphogenesis; Phaseolus vulgaris L.; Plant growth regulators.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582010000100006

Establishment of a pheasant (Phasianus colchicus) spermatogonial stem cell line for the production of interspecies germ line chimeras
Provedor de dados: 69
Autores: Kim,Jeong Hyun; Sharma,Neelesh; Kim,Sung Woo; Sodhi,Simrinder Singh; Ghosh,Mrinmoy; Kim,Nam Eun; Mongre,Raj Kumar; Oh,Sung Jong; Jeong,Dong Kee.
Background Spermatogonial stem cells (SSCs) are important for the production of interspecies germ line chimeras. The interspecies germ cell transfer technique has been suggested as a way to conserve endangered birds. Our objective was to develop a technique for restoring endangered birds by developing interspecies germ line chimeras between pheasant (Phasianus colchicus) and chicken (Gallus gallus) with SSCs. Results SSCs were isolated from the surgically removed testis of a pheasant. Growth conditions for pheasant SSCs were established by co-culturing STO (SIM mouse embryo-derived thioguanine and ouabain resistant) cells and pheasant SSCs. The colony-forming cells divided and proliferated stably to yield an established SSC line. Pheasant SSCs showed...
Tipo: Journal article
Palavras-chave: Endangered birds; In vitro culture; Recipient chicken embryos; SSC transplantation.
Ano: 2014
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582014000500004

Micropropagation of Homalomena pineodora Sulaiman &amp;amp; Boyce (Araceae): a new species from Malaysia
Provedor de dados: 75
Autores: Stanly,Christine; Bhatt,Arvind; Sulaiman,Baharuddin; Keng,Chan Lai.
Homalomena pineodora (family Araceae) is a species found to have impressive foliage characteristics which remain evergreen throughout the year. Therefore, H. pineodora can be grown as an ornamental plant. Generally H. pineodora needs 3-5 years to propagate and multiply. However, the demand for new ornamental plants is increasing worldwide and the quality of planting material is a basic need for boosting productivity. Therefore an efficient micropropagation protocol for large-scale production of H. pineodora was developed. In vitro shoot cultures were initiated from the rhizomatous buds on MS basal medium. The best conditions for propagating H. pineodora was found to be MS medium supplemented with 3% sucrose and 0.5 mg L-1 BA (6-benzyladenine) under 24 h of...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Homalomena pineodora; Acclimatization; Scanning electron microscopy; Cytokinin; In vitro culture; Shoot proliferation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000100007

In vitro organogenesis of zucchini squash cv. Caserta
Provedor de dados: 75
Autores: Stipp,Liliane Cristina L; Monteiro-Hara,Alessandra Cristina BA; Mendes,Beatriz Madalena J.
A protocol for the in vitro culture of Cucurbita pepo cv. Caserta was studied, using a cotyledon segment with an attached hypocotyl fragment as an explant. First, to determine the optimal seedling age, explants were collected from 4 to 6-day-old in vitro germinated seedlings and cultured in MS basal medium supplemented with benzylaminopurine (BAP, 4.5 µM), under a 16-h photoperiod at 27ºC. Based on the results obtained, the explants collected from the 4-day-old seedlings were then cultured in MS basal medium supplemented with different concentrations of BAP (0, 1.1, 2.2, 3.3, 4.5, or 5.5 µM) and incubated under a 16-h photoperiod at 27ºC. In vitro organogenesis was most efficient with explants collected from 4-day-old seedlings cultured in medium...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Adventitious buds; Cotyledon attached to hypocotyl; Cucurbita pepo; In vitro culture.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362012000200015

Seed disinfestation methods for in vitro cultivation of epiphyte orchids from Southern Brazil
Provedor de dados: 75
Autores: Alvarez-Pardo,Veronica M; Ferreira,Alfredo Gui; Nunes,Valter F.
Disinfestation of orchid seeds is necessary when the germination is performed in vitro. In such case, cultures are supplemented by a source of sugar and salts in order to obtain energy and other requirements. However, the presence of sugar and salts easily allows the development of unwanted microorganisms. To avoid such phenomenon, an efficient procedure must be done as a pretreatment of the seeds with liquid or gaseous substances. In this study with several Brazilian orchids, the seeds were disinfested with sodium hypochlorite solutions containing 0.4% or 0.8% active chlorine for five minutes and sodium hypochlorite or formaldehyde fumes up to two hours, this procedure being efficient for such purpose and making it possible to be used routinely.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Orchids; In vitro culture; Germination; Sodium hypoclorite; Formaldehyde.
Ano: 2006
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-05362006000200019

Melhoramento de plantas de interesse econômico para a região amazônica através de técnicas in vitro.
Provedor de dados: 14
Autores: MOTA, M. G. da C.; LEMOS, O. F. de; POLTRONIERI, M. C.; MENEZES, I. C.
bitstream/item/60200/1/CPATU-PA149.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Melhoramento de planta; Pimenta-do-reino; Castanha do brasil; Brasil.; Bacuri; Cultura In Vitro; Cultura de Tecido; Guaraná; Paullinia Cupana; Piper Nigrum; Platonia Insignis.; Brazil nuts; Amazonia; In vitro culture; Plant breeding; Tissue culture.
Ano: 1991
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/374123

Potencial agrícola de bactérias endofíticas obtidas de bambu Guadua spp.
Provedor de dados: 14
Autores: VALE, S. M. L. S. do; VALE, P. A. A. do; CARVALHO, C. M.; SIVIERO, A.
Esta pesquisa teve por objetivo caracterizar as bactérias endofíticas de Guadua spp. e estimar o seu potencial de uso agrícola como solubilizados de fosfato e fixação de nitrogênio.
Tipo: Capítulo em livro técnico (INFOTECA-E)
Palavras-chave: Sustancias de crecimiento vegetal; Solubilización.; Fijación del nitrógeno; Bactéria endofítica; Endophytic bacteria; Cultivo in vitro; Guadua chaparensis; Guadua weberbeauri; Bambu; Fosfato.; Solubilização; Estimulante de Crescimento Vegetal; Cultura In Vitro; Fixação de Nitrogênio; Biofertilizante; Nitrogen fixation; Phosphates.; Bamboos; Guadua; Plant growth substances; In vitro culture; Solubilization.
Ano: 2019
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1119043

Protocolo de micropropagação para as espécies Piper hispidinervum e P. aduncum.
Provedor de dados: 14
Autores: RAPOSO, A.; TEIXEIRA, R. B.; MANFIO, C. E.; SILVA, D. A. da; MOREIRA, N. R. da S.; MACIEL, S. de A.
Este trabalho apresenta o protocolo de cultivo in vitro para as espécies Piper hispidinervum e P. aduncum, utilizando material oriundo de mudas obtidas por meio da germinação in vitro de sementes.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Técnicas de laboratorio.; Cultivo in vitro; Micropropagación; Pimenta de macaco.; Micropropagação; Cultura in vitro; Pimenta longa; Piper hispidinervum; Piper longum; Micropropagation; In vitro culture; Laboratory techniques.; Piper aduncum.
Ano: 2012
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/943350

Produção in vivo e recuperação não cirúrgica de embriões ovinos no Brasil.
Provedor de dados: 14
Autores: FONSECA, J. F. da.
Esta circular técnica sumariza resultados de produção in vivo de embriões ovinos coletados especificamente pela via transcervical (NSER) no Brasil, que dão suporte à recomendação do Protocolo Embrapa de Superovulação em várias raças ovinas.
Tipo: Folhetos
Palavras-chave: Ovino; Reprodução Animal; Cultura de Embrião; Superovulação; Sheep; Animal reproduction; In vitro culture; Embryo culture; Brazil.
Ano: 2021
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/1137612

Cultura in vitro de embriões zigóticos de cupuaçuzeiro.
Provedor de dados: 14
Autores: LEDO, A. da S.; LAMEIRA, O. A.; MOURA, E.; MENEZES, I. C. de.
bitstream/item/42519/1/Boletim-Pesquisa-28.pdf
Tipo: Séries anteriores (INFOTECA-E)
Palavras-chave: Cupuaçuzeiro; Belém; Pará; Brasil.; Cultura de Tecido; Cultura In Vitro; Micropropagação; Theobroma Grandiflorum.; Amazonia; In vitro culture; Micropropagation; Tissue culture.
Ano: 2000
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/382729

Metodologia científica: cultivo in vitro de unha-de-gato.
Provedor de dados: 14
Autores: RAPOSO, A.; TEIXEIRA, R. B.
O uso da unha-de-gato como produto comercial (fitoterápico) apresenta algumas dificuldades, entre elas a falta de homogeneidade na constituição química das plantas comercializadas. Esse problema ocorre pelo fato da matéria-prima ser proveniente de áreas naturais sem nenhum controle de qualidade. Nesse sentido, a tecnologia de clonagem in vitro, apoiada por um trabalho de seleção de características desejáveis, poderá contribuir para a obtenção de plantas com maior homogeneidade e de qualidade garantida.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Cultivo in vitro; Plantas medicinales.; Micropopagation; Unha de Gato.; Planta medicinal; Cultura In Vitro; Micropropagação; In vitro culture; Uncaria guianensis.; Medicinal plants; Uncaria tomentosa.
Ano: 2011
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/912085

Embriogênese somática e regeneração de plantas em açaizeiro.
Provedor de dados: 14
Autores: LEDO, A. da S.; LAMEIRA, O. A.; MENEZES, I. C. de.
O objetivo do presente trabalho foi estudar as diferentes respostas morfogenéticas de embriões zigóticos de açaizeiro (Euterpe oleracea Mart.) submetidos a várias condições de cultura in vitro. Os experimentos foram conduzidos em laboratório com material vegetal coletado de plantas de açaí na Embrapa Amazônia Oriental, Belém, PA, Brasil. Verificou-se a expressão de um modelo de embriogênese somática, direto, repetitivo a assincronizado em embriões zigóticos maduros cultivados em meio primário MS suplementado com 339.36 ? M de 2,4-diclorofenoxiacético (2,4-D) e transferidos para meio secundário MS na presença de 0,537 ? M de ácido 1-naftalenoacético (ANA) e 12,30 ? M de 2-isopenteniladenina (2iP). A conversão de embriões somáticos em plântulas foi alcançada...
Tipo: Boletim de Pesquisa e Desenvolvimento (INFOTECA-E)
Palavras-chave: Açaí de touceira; Embrapa Amazônia Oriental; Belém (PA); Pará; Eastern Amazon; Amazônia Oriental; Micropropagación.; Cultivo in vitro; Fitomejoramiento; Embriogénesis; Melhoramento genético vegetal; Açaí; Euterpe oleracea; Cultura in vitro; Embriogênese.; Micropropagação; Embryogenesis.; Micropropagation; Plant breeding; In vitro culture.
Ano: 2002
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/496030

Produção de mudas de teca por micropropagação.
Provedor de dados: 14
Autores: RAPOSO, A.; FERMINO JUNIOR, P. C. P.; TEIXEIRA, R. B.; PEREIRA, J. E. S.
A propagação in vitro de teca tem sido realizada por meio da cultura de tecidos vegetais de diferentes genótipos. Nesse contexto, o uso da micropropagação a partir de plantações de teca no Brasil pode ser uma importante ferramenta, pois permite a produção de grandes quantidades de novas plantas em um curto espaço de tempo, a partir de um único propágulo vegetativo retirado de uma planta matriz. Assim, o objetivo deste trabalho é descrever protocolos de micropropagação da espécie Tectona grandis a partir de genótipos estabelecidos na Amazônia Sul-Ocidental, no Estado do Acre.
Tipo: Circular Técnica (INFOTECA-E)
Palavras-chave: Teak; Producción de plántulas.; Cultivo in vitro; Madera tropical; Micropropagación; Tectona grandis; Muda; Produção.; Cultura in vitro; Essência florestal; Teca; Micropropagação; Tropical wood; Seedling production.; In vitro culture; Micropropagation.
Ano: 2010
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/872550

Protocolo para a propagação in vitro de bananeira cultivar prata zulu.
Provedor de dados: 14
Autores: QUISEN, R. C.; MARI, A. de O.; LOPES, C. de O.
Este trabalho tem por objetivo relatar, de forma simplificada, a partir de resultados de pesquisas realizadas no Laboratório de Biotecnologia da Embrapa Amazônia Ocidental, Manaus, as melhores condições de produção in vitro de mudas da banana cv. Prata Zulu, cultivar que tem sobressaído às demais pela alta resistência à sigatoka-negra e pelos bons resultados fitotécnicos apresentados.
Tipo: Comunicado Técnico (INFOTECA-E)
Palavras-chave: Mal de sigatoka; Brasil; Amazonas; Manaus; Species; Planting stock; Plant diseases.; Cultura In Vitro; Doença de Planta; Espécie; Muda; Propagação Vegetativa; Variedade.; Musa; In vitro culture; Varieties; Vegetative propagation.
Ano: 2001
URL: http://www.infoteca.cnptia.embrapa.br/infoteca/handle/doc/671309

Etude d'une prolifération hémocytaire chez la moule Mytilus edulis
Provedor de dados: 5
Autores: Noel, Danièle.
Hemic proliferative disease in the bay mussel, Mytilus edulis, has been studied following mortalities in Washington State (USA). The disease has been reproduced in the labortory. The establishment of this experimental model led to study tumoral characteristics of the atypic hemocytes : ultrastrure, in vitro cultivation, transplantation. The infectious etiology of this tumor has been verified. It has been hypothesized to result from a viral infection since retrovirus-like particules have been observed following induction by bromodeoxyuridine. Specific monoclonal antibodies against atypic hemocytes have been obtained and characcterized
Tipo: Text
Palavras-chave: Mytilus; Mollusque; Hémocyte; Tumeur; Ulstrastructure; In vitro culture; Transplantation; Rétrovirus; Anticorps monoclonaux; Mytilus; Mollusca; Hemocyte; Tumor; Ultrastructure; In vitro cultivation; Transplantation; Retrovirus; Monoclonal antibodies.
Ano: 1988
URL: http://archimer.ifremer.fr/doc/00428/53930/55057.pdf

Embryogenic Callus induction on the scutellum and regeneration of plants as basis for genetic transformation of spring wheath (Triticum Aestivum L.) cultivars from Argentina
Provedor de dados: 46
Autores: Souza Canada,E. D; Beck,E.
An effective tissue culture is an indispensable basis for the production of transgenic plants. In this study, we investigated the induction of embryogenic callus production from the scutellum of spring wheat and subsequent regeneration of plants. Twenty-two Argentine spring wheat varieties from three breeders were screened to select promising genotypes for the optimization of the procedure. Experimental variables were the developmental stage of the immature embryos and the concentration of the growth regulator 2,4-dichlorophenoxyacetic acid (2,4-D). Two developmental stages, termed H and W, respectively (according to He et al., 1986), were selected, and their performance in culture media containing high and low concentrations of 2,4-D was examined....
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Immature embryos; Embryogenic scutellar callus; Callus proliferation.
Ano: 2013
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1852-62332013000300006

Experimental taxonomy exposes ontogenetic variability and elucidates the taxonomic value of claw configuration in Milnesium Doyère, 1840 (Tardigrada: Eutardigrada: Apochela)
Provedor de dados: 16
Autores: Witold Morek, W.; Gąsiorek, P.; Stec, D.; Blagden, B.; Michalczyk, Ł.
In this paper we describe a new apochelan species, Milnesium variefidum sp. nov. from Scotland and provide novel morphological and molecular data for Milnesium berladnicorum Ciobanu et al., 2014. The new species differs from the most similar M. berladnicorum by the presence of developmental dimorphism in claw configuration, absent or weakly developed cuticular bars under claws I-III, a different arrangement of cuticular pseudoplates, and by differences in the sequences of three nuclear DNA fragments: 18S rRNA (p-distance: 0.6%), 28S rRNA (2.0%), ITS-2 (9.3%), and on mitochondrial gene COI (12.4%). Although ontogenetic claw configuration change was suspected to occur in some Milnesium species, we are the first to document it through the combined use of...
Tipo: Article / Letter to the editor
Palavras-chave: Barcoding; Diagnostic key; Development tracking; In vitro culture; M. berladnicorum; M. variefidum sp. nov.
Ano: 2016
URL: http://www.repository.naturalis.nl/record/607686

Isolation, cultivation and molecular characterization of a new Trypanosoma equiperdum strain in Mongolia
Provedor de dados: 19
Autores: Suganuma, Keisuke; Narantsatsral, Sandagdorj; Battur, Banzragch; Yamasaki, Shino; Otgonsuren, Davaajav; Musinguzi, Simon Peter; Davaasuren, Batdorj; Battsetseg, Badgar; Inoue, Noboru.
Background: Trypanosoma equiperdum causes dourine via sexual transmission in Equidae. T. equiperdum is classified under the subgenus Trypanozoon along with the T. brucei sspp. and T. evansi; however, the species classification of Trypanozoon remains a controversial topic due to the limited number of T. equiperdum reference strains. In addition, it is possible that some were misclassified T. evansi strains. Thus, there is a strong need for a new T. equiperdum strain directly isolated from the genital mucosa of a horse with a clinically-and parasitologically-confirmed dourine infection. Methods: Trypanosomes isolated from the urethral tract of a stallion with suspected dourine, were directly cultivated using soft agarose media at 37 degrees C in 5 % CO2. For...
Palavras-chave: Dourine; In vitro culture; Maxicircle DNA; Mongolia; Soft agarose media; Trypanosoma equiperdum.
Ano: 2016
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/4442

In vitro inhibitory effect of fosmidomycin on the asexual growth of Babesia bovis and Babesia bigemina
Provedor de dados: 19
Autores: Sivakumar, T; Aboulaila, M. R. A.; Khukhuu, A; Iseki, H; Alhassan, A; Yokoyama, Naoaki; Igarashi, Ikuo; 横山, 直明; 五十嵐, 郁男.
The in vitro inhibitory effect of fosmidomycin was evaluated against the asexual growth of bovine Babesia parasites (Babesia bovis and Babesia bigemina). In the cultures with these parasites, severe reduction of parasite growth was observed by the addition of 1.25 μg/ml of the final drug concentration. Under these conditions, B. bigemina was completely cleared until the third day of cultivation. On the other hand, B. bovis was completely eliminated on the fourth day when the concentration was 6.25 μg/ml in the culture. These parasites failed to grow again when a drug-free medium was substituted for the subsequent cultivation. The IC50 values of fosmidomycin against B. bovis were determined as 0.88 and 0.63 μg/ml in the in vitro cultures with...
Palavras-chave: Fosmidomycin; Babesia bovis; Babesia bigemina; Asexual growth; In vitro culture.
Ano: 2008
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2235

Comparative Evaluation of Mehods for the Diagnosis of Bovine Tritrichomonas foetus Infection
Provedor de dados: 19
Autores: Felleisen, Richard S. J.; Schimid-Lambelet, Natacha; Walubengo, J.
Palavras-chave: Diagnosis; In vitro culture; Polymerase chain reaction (PCR).
Ano: 1997
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/266

Continuous in vitro Cultivation of Babesia ovata
Provedor de dados: 19
Autores: Igarashi, Ikuo; Avarzed, Avgaandorjiin; Tanaka, Tetsuya; Inoue, Noboru; Ito, Masanari; Omata, Yoshitaka; Saito, Atsushi; Suzuki, Naoyoshi.
Palavras-chave: Babesia ovata; In vitro culture; Low oxygen atmosphere; Cryopreservation.
Ano: 1994
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/212

In vitro conservation of apical meristem-tip of Melia azedarach L. (Meliaceae) under slow-growth conditions: (with 1 table &amp;#38; 2 figures)
Provedor de dados: 85
Autores: Scocchi,AM; Mroginski,LA.
Studies on in vitro storage of Melia azedarach -"paradise tree"- under slow-growth conditions were carried out to develop an efficient protocol for the conservation of the genetic diversity of the crop. The response to different growth retardation treatments was examined using clone E. In vitro cultures of apical meristem-tips (0.2-0.3 mm in length) could be effectively maintained for 12 months at 4ºC on ¼ MS basal medium supplemented with 0.5 µM BA. Shoot proliferation was observed on 67 % of the recovered apical meristem-tips, after 12 months at 4ºC. Rooting of the regenerated shoots was achieved by reculturing them on MS + 17.5 µM IBA, during 4 days and subsequently transferred to MS medium lacking growth-regulators for 30 days. Complete plants were...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Conservation; Germplasm; In vitro culture; Melia azedarach; Meristem.
Ano: 2004
URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S1851-56572004000100017

Laboratory Protocols 2008: Maize Nutrition Quality and Plant Tissue Analysis Laboratory
Provedor de dados: 31
Autores: Gallicia,   L.; Nurit,   E.; Rosales,   A.; Palacios-Rojas,   N.
Tipo: Report
Palavras-chave: Zea mays; Maize; Wheats; Triticum; Transgenic plants; Genetic engineering; Gene transfer; Genetic transformation; DNA hybridization; Phenotypes; Selection; Tissue culture; In vitro culture; Biolistics; Research projects; Methods; Molecular cloning; PCR; Crop Production/Industries; F02; F30.
Ano: 2009
URL: http://purl.umn.edu/56177

Estudo de nutrição mineral in vitro relacionado à adaptação de Sinningia allagophylla (Martius) Wiehler (Gesneriaceae) às condições de cerrado
Provedor de dados: 88
Autores: NOVAES GOMES,MÁRCIA A.; SHEPHERD,SIMONE L. KIRSZENZAFT.
O estudo in vitro foi realizado a partir de sementes de S. allagophylla, colhidas de plantas crescidas na Reserva Biológica de Moji-Guaçu. Foram testadas duas interfaces da adaptação desta espécie às condições do cerrado: efeito do pH e das concentrações de nutrientes, utilizando o meio básico de Murashige &amp;amp; Skoog (MS) e o de Gamborg et al. (B5). Modificações do meio MS foram feitas em relação ao pH, com um gradiente de valores iniciais, indo do 4,2 ao 5,8 (intervalos de 0,2), e em relação aos nutrientes KNO3, KH2PO4 e MgSO4.7H2O, com concentrações progressivamente menores destes. Quanto ao meio B5 foi testada a composição nutricional nas concentrações totais e reduzidas à metade (B5 50%). Os resultados mostraram que as adaptações desta espécie do...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Acid pH; Cerrado; Gesneriaceae.
Ano: 2000
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84042000000200005

Dry matter partitioning differences between shoots and roots in two contrasting genotypes of orchids and their relationship with endogenous levels of auxins, cytokinins and abscisic acid
Provedor de dados: 93
Autores: PERES,LÁZARO E. P.; MAJEROWICZ,NIDIA; KERBAUY,GILBERTO B.
Epiphytic orchids have been considered an interesting model for plant development and may have unusual features related to the hormonal control of shoot and root growth. In the present experiment we have studied two genotypes of Catasetum fimbriatum Lindl. (Orchidaceae) with an inverse pattern of shoot and root dry matter accumulation. Whereas the CFC1 genotype directs 60% of dry matter towards the shoot system, this same amount is directed towards the root system in the CFC4 genotype. Differences in the endogenous content of cytokinins, IAA and ABA, as well as differences in IAA conjugation and oxidation in shoots and roots were observed in the two genotypes. These differences were correlated with their dry matter partitioning patterns. Thus, the genotype...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Epiphytes; Development; In vitro culture; Orchids; Plant hormones.
Ano: 2001
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-31312001000200007

Optimization of a protocol for the micropropagation of pineapple
Provedor de dados: 94
Autores: ALMEIDA,WELITON ANTONIO BASTOS DE; SANTANA,GESSIONEI SILVA; RODRIGUEZ,ADRIANA PINHEIRO MARTINELI; COSTA,MARIA ANGÉLICA PEREIRA DE CARVALHO.
The present work aimed at maximizing the number of plantlets obtained by the micropropagation of pineapple (Ananas comosus (L.) Merrill) cv. Pérola. Changes in benzylaminopurine (BAP) concentration, type of medium (liquid or solidified) and the type of explant in the proliferation phase were evaluated. Slips were used as the explant source, which consisted of axillary buds obtained after careful excision of the leaves. A Sterilization was done in the hood with ethanol (70%), for three minutes, followed by calcium hypochlorite (2%), for fifteen minutes, and three washes in sterile water. The explants were introduced in MS medium supplemented with 2mg L-1 BAP and maintained in a growth room at a 16h photoperiod (40 mmol.m-2.s-1), 27 ± 2ºC. After eight weeks,...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus; Tissue culture; In vitro culture; In vitro clonal propagation.
Ano: 2002
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452002000200005

Illustrated guide to the classification of banana seeds and embryos
Provedor de dados: 94
Autores: Silva,Manassés dos Santos; Rodrigues,Taise Conceição; Aud,Fabiana Ferraz; Santos-Serejo,Janay Almeida dos; Amorim,Edson Perito.
Abstract  Considering there is no illustrated scale of the principle anomalies observed for banana seeds, the objective of this guide is to cover in detail, for the first time, the categories to classify the most common types of anomalies observed in banana embryos and seeds, as well as in vitro embryo rescue and culture, taking into account the experience acquired by the banana breeding program at Embrapa in Brazil. Four classes to classify the seeds were proposed and with regards to classifying the embryos, eight classes were established.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Germination; Genetic improvement; Musa spp; Embryo rescue.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452019000210001

Residual effect of growth regulators in etiolation and regeneration of in vitro pineapple plants
Provedor de dados: 94
Autores: Souza,Fernanda Vidigal Duarte; Canto,Ana Maria Mascarenhas Eloy; Souza,Antônio da Silva; Costa,Maria Angélica Pereira de Carvalho.
This work aimed to evaluate the influence of naphthaleneacetic acid (NAA) and gibberellic acid (GA3) plant regulators in in vitro etiolation and subsequent regeneration of the PE x SC-60 pineapple hybrid. Nodal segments of in vitro plants with approximately 5-7 cm height were incubated in basic MS culture medium supplemented with 0.0; 0.5 and 1.0 mg L-1 of naphthaleneacetic acid (NAA) in combination with gibberellic acid (GA3) in concentrations of 0.0; 0.5 and 1.0 mg L-1, and maintained at 27 ºC under dark condition. Evaluations were carried out at 90 and 180 days after incubation period. The best results for length of etiolated stems were obtained with 1.0 mg L-1 of NAA. In the experiment followed by the regeneration, stems with 3 cm from the etiolation...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Ananas comosus var. comosus; In vitro culture; Plant regulators; Nodal segments; Germplasm conservation; Micropropagation.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-29452010000200035

Effects of green tea polyphenols, insulin-like growth factor I and glucose on developmental competence of bovine oocytes
Provedor de dados: 96
Autores: Wang,Zhengguang; Fu,Chunquan; Yu,Songdong.
The present study examined the effects of green tea polyphenols (GTP), insulin-like growth factor-I (IGF-I) and glucose on oocyte in vitro maturation, subsequent embryo development and blastocyst quality in bovine. Cumulus-oocyte complexes (COC) were aspirated from the ovaries and cultured in synthetic oviduct fluid supplemented with MEM amino acids (SOFaa) media supplemented with one of the following supplements: GTP (0, 10, 15 and 20 µM), IGF-I (0, 50, 100 and 150 ng/mL) or glucose (0, 1.5, 5.6 and 20 mM) for 24 h. The results showed that oocytes cultured in media supplemented with 15 µM GTP, 100 ng/mL IGF-I and 5.6 mM glucose, in separate experiments, have higher cleavage and blastocyst rates compared with oocytes cultured in media without or with other...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Blastocyst quality; Embryo development; In vitro culture; In vitro maturation.
Ano: 2012
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982012001200006

The effects of ovalbumin as a protein source during the in vitro production of bovine embryos
Provedor de dados: 96
Autores: Tetzner,Tatiane Almeida Drummond; Saraiva,Naiara Zoccal; Perecin,Felipe; Niciura,Simone Cristina Méo; Ferreira,Christina Ramires; Oliveira,Clara Slade; Garcia,Joaquim Mansano.
Embryo quality is influenced by the culture conditions that affect in vitro maturation (IVM), fertilization (IVF) and culture (IVC) rates. The present study investigated the feasibility of producing bovine embryos after the replacement of fetal calf serum (FCS) and bovine serum albumin (BSA) by ovalbumin (OVA). The IVM and IVC medium were supplemented with 10% FCS, 4 mg/mL BSA, or 4 mg/mL OVA. The IVF medium was supplemented with 6 mg/mL BSA or OVA. For IVM, supplementation with FCS, BSA, and OVA did not affect nuclear maturation or cortical granule migration. Higher rates of formation of two pronuclei were obtained when FCS was employed for IVM (79.97%), regardless of the supplement used for IVF, and when BSA was used for IVF (59.4%), regardless of the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine embryo; In vitro culture; Ovalbumin; Protein source.
Ano: 2011
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-35982011001000010

In vitro germination and viability of pollen grain of coconut accessions1
Provedor de dados: 124
Autores: Moura,Catrine Regina Feitosa; Machado,Caroline de Araújo; Lédo,Ana da Silva.
Storage as a means of maintaining the pollen viability is important for the preservation of the genetic variability, facilitates the exchange of germplasm and greatly contributes to the generation of variability obtained from artificial crosses, increasing the efficiency of breeding programs. The objective of this study was to select different culture media for the in vitro germination of pollen grain of dwarf and tall coconut accessions, as well as to determine the viability of pollen grain at room temperature conditions. For this purpose, Brazil Green Dwarf (BGD) and Brazilian Tall (BRA) pollen grains derived from the Coconut Active Germplasm Bank of Embrapa Coastal Tablelands Sergipe were used. To evaluate the effect of different culture media on the in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; In vitro culture; Culture media.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902015000200421

Callus induction and bioactive phenolic compounds production from Byrsonima verbascifolia (L.) DC. (Malpighiaceae)
Provedor de dados: 124
Autores: Castro,Ana Hortência Fonsêca; Braga,Karina de Queiroz; Sousa,Fernanda Marinez de; Coimbra,Mairon César; Chagas,Rafael César Russo.
ABSTRACT This study developed a methodology for callus induction in leaf segments of B. verbascifolia and evaluated the bioactive phenolic compounds production. Leaf explants were cultured in MS medium with 30 g L-1 sucrose, solidified with 7 g L- 1 agar supplemented with 2,4-D (0; 4.52; 9.05; 18.10 µM) and BAP (0; 4.44; 8.88; 17.75 µM ) in the presence and absence of light. Forty-five days after inoculation we assessed the percentage of callus induction, color, consistency, fresh and dry matter, total phenols, flavonoids, tannins contents, and chromatographic profile by HPLC-DAD method. Callus induction occurred only in medium with growth regulators. Maximal induction (100%) was found in medium containing 2,4-D combined with BAP in the presence and...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cerrado; Medicinal plant; In vitro culture; Secondary metabolites.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1806-66902016000100143

Plant regeneration from cotyledonary explants of Eucalyptus camaldulensis
Provedor de dados: 63
Autores: Dibax,Roberson; Eisfeld,Cristiane de Loyola; Cuquel,Francine Lorena; Koehler,Henrique; Quoirin,Marguerite.
Breeding methods based on genetic transformation techniques need to be implemented for Eucalyptus camaldulensis to shorten the long breeding cycles and avoid manipulation of adult trees; that requires the development of plant regeneration protocols enabling development of plants from transformed tissues. The present work aimed to optimise the regeneration process already established for the species. Cotyledonary leaves of E. camaldulensis were cultured in MS medium supplemented with naphthaleneacetic acid (NAA) and 6-benzylaminopurine (BAP) combinations. The most efficient treatment for bud indirect regeneration (2.7 µmol L-1 NAA and 4.44 µmol L-1 BAP) was used for further experiments. When explants were kept in the dark during the first 30 days, the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Forest species; Culture media; Organogenesis.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162005000400016

Plant regeneration from proroplasts of alfalfa (Medicago sativa) via somatic embryogenesis
Provedor de dados: 63
Autores: Monteiro,Mariza; Appezzato-da-Glória,Beatriz; Valarini,Maria José; Oliveira,Carlos Alberto de; Vieira,Maria Lucia Carneiro.
Alfalfa is one of the most frequently studied species from the production of tissue culture-derived embryos point of view. In this study, five alfalfa cultivars were analyzed with reference to their ability to regenerate plants from protoplast cultures via somatic embryogenesis. Plant regeneration from leaf-derived protoplasts isolated from the cultivar Rangelander was achieved using a protocol defined for alfalfa cell suspension-derived embryogenesis. Because of its high efficiency, this procedure is recommended for protoplast electroporation-mediated genetic transformation of alfalfa.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Plant biotechnology; In vitro culture; Morphogenesis; Forage legume.
Ano: 2003
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162003000400012

Micropropagation and callogenesis of Curcuma zedoaria Roscoe
Provedor de dados: 63
Autores: Miachir,Jeanette Inamine; Romani,Vera Lúcia Moretti; Amaral,Antônio Francisco de Campos; Mello,Marcia Ometto; Crocomo,Otto Jesu; Melo,Murilo.
Curcuma zedoaria Roscoe (zedoary) is a medicinal properties-bearing Zingiberaceae from which rhizomes are commercially exploited. The objective of this work was to establish an in vitro protocol for micropropagation and callogenesis of Curcuma zedoaria Roscoe as alternative to improve plant production, turning economically feasible the exploitation of its secondary metabolites which present medicinal properties. Micropropagation by using shoot apexes produced by rhizome and from in vitro plants were carried out on Murashige &amp;amp; Skoog medium supplemented with 2.0 mg L-1 benzyl amino purine and 30 g L-1 sucrose. Plantlets were satisfactorily acclimated to greenhouse conditions by using plastic cover for at least 10 days. Treatment with endomycorrhiza...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Zingiberaceae; In vitro culture; Plant production; Cells suspension; Callus.
Ano: 2004
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162004000400012

RESPONSE TO in vitro SALT STRESS IN SUGARCANE IS CONDITIONED BY CONCENTRATION AND CONDITION OF EXPOSURE TO NaCI
Provedor de dados: 37
Autores: CAVALCANTE GRANJA,Manuela Maria; LACERDA E MEDEIROS,Maria Jaislanny; MEDEIROS DE ANDRADE SILVA,Marina; CAMARA,Terezinha; WILLADINO,Lilia; ULISSES,Claudia.
ABSTRACT Salinity is one of the major environmental stress factors that affect crop productivity, as well as interfering with plant growth and development, resulting in reduced production quality. Given this, we highlight the importance of research in response to plants subjected to salt stress in order to assess the physiological and biochemical behavior of genotypes, with the objective of selecting the more tolerant. One way to ensure the uniformity of the response of the plants is through in vitro cultivation, which allows control of the cultivation conditions. Therefore, the objective of this work was to evaluate the response of two commercial varieties of sugar cane exposed to saline stress in different conditions (gradual and abrupt). Two varieties...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Antioxidant enzymes; In vitro culture; Oxidative stress; Salinity.
Ano: 2018
URL: http://www.scielo.org.co/scielo.php?script=sci_arttext&pid=S0120-548X2018000100030

Asymbiotic culture of Cattleya intermedia Graham (Orchidaceae): the influence of macronutrient salts and sucrose concentrations on survival and development of plantlets
Provedor de dados: 39
Autores: Sasamori,Márcio Hisayuki; Endres Júnior,Delio; Droste,Annette.
ABSTRACTCattleya intermediais an Atlantic Forest species endemic to Brazil that is classed as vulnerable on the list of threatened species. In this study, C. intermedia plantlets were micropropagated in an asymbiotic culture and the influence of different concentrations of sucrose (15, 30, 45 and 60 g L-1, plus a zero sucrose medium) and macronutrient salts (complete Murashige and Skoog (MS) medium and half MS medium (with half-strength macronutrients)) on survival and development of the plantlets was evaluated. In all treatments 100% plantlet survival was achieved. The integrated analysis of height of aerial part, number of leaves per plantlet, fresh mass, number of roots per plantlet and length of the longest root showed that the plantlets exhibited...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Atlantic Forest; Carbohydrate; Conservation; In vitro culture; Orchids; Propagation.
Ano: 2015
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-33062015000300292

In vitro culture of Gigaspora decipiens and Glomus clarum in transformed roots of carrot: the influence of temperature and pH - doi: 10.4025/actasciagron.v35i3.16581
Provedor de dados: 1
Autores: Costa, Francilina Araújo; Universidade Católica Dom Bosco; Haddad, Lydice Sant’Anna Meira; Universidade Federal do Recôncavo da Bahia; Kasuya, Maria Catarina Megumi; Universidade Federal de Viçosa; Otoni, Wagner Campos; Universidade Federal de Viçosa; Costa, Maurício Dutra; Universidade Federal de Viçosa; Borges, Arnaldo Chaer; Universidade Federal de Viçosa.
Monoxenic cultures of arbuscular mycorrhizal fungi (AMF) in transformed roots have been used to better understand the symbiosis with these fungi, but few species have been successfully established in vitro. The object was to establish monoxenic cultures of Gigaspora decipiens and Glomus clarum and to verify the effects of temperature and pH on spore formation. Cultures were established from superficially disinfested spores that were germinated on an agar and water. After germination, the spores were transferred to Petri dishes containing transformed carrot roots. After 4-6 days formed newly formed spores and structures typical. The effects of temperature (22, 25, 28 or 32°C) and pH (4.0, 4.5, 5.5 or 6.5) on the production of spores were assessed over three...
Tipo: Pesquisa em laboratório
Palavras-chave: 5.01.01.04-8 arbuscular mycorrhizal fungi; Monoxenic culture; In vitro culture; Monoxenic inoculum; Transformed roots Microbiologia Agrícola.
Ano: 2013
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/16581

Leaf anatomy of orchids micropropagated with different silicon concentrations - doi: 10.4025/actasciagron.v34i4.15062
Provedor de dados: 1
Autores: Soares, Joyce Dória Rodrigues; Universidade Federal de Lavras; Pasqual, Moacir; Universidade Federal de Lavras; Araujo, Aparecida Gomes de; Universidade Federal de Lavras; Castro, Evaristo Mauro de; Universidade Federal de Lavras; Pereira, Fabricio José; Universidade Federal de Lavras; Braga, Francyane Tavares; Universidade Federal de Lavras.
Research on anatomical modifications under in vitro culture is essential to the definition and understanding of the development of micropropagated plants. Likewise, such research is essential to improve the steps of the acclimatization process. Accordingly, the objective of the present study is to verify the differences in the leaves anatomical traits of micropropagated orchids under calcium silicate concentrations. Seedlings of in vitro-germinated seeds, measuring 0.5 cm in length, were inoculated in 250 cm3 pots with 60 mL MS culture medium and different silicon concentrations (0, 0.5, 1.0 and 2.0 mg L-1) in different culture environments (natural environment, in a greenhouse and an artificial environment in a growth chamber) and in all statistical...
Palavras-chave: Microscopy; Orchidaceae; In vitro culture; Calcium silicate microscopy; Orchidaceae; In vitro culture; Calcium silicate.
Ano: 2012
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/15062

Germination and gametophyte development of Cyathea corcovadensis (Raddi) Domin (Cyatheaceae) from spores stored at low temperatures
Provedor de dados: 3
Autores: Marcon, Catiuscia; Silveira, Tatieli; Droste, Annette.
The tree fern Cyathea corcovadensis (Raddi) Domin is an endangered species in the state of Rio Grande do Sul, Brazil. It currently occurs only in the northern segment of the coastal region. Spore storage would help in conservation programs since it maintains genetic variability and provides material for in vitro cultures. Current study evaluates the effect of low temperatures combined to different spore storage times on the germination and initial gametophyte development of C. corcovadensis. Spores were divided into two groups: spores of the first group were sowed immediately in Meyer culture medium with nystatin, at pH 4.0, while spores of the second group were stored at 7, -20 and -196°C during 60, 120, 180, and 365 days and then sowed in the same...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.00.00.00-6 conservation; In vitro culture; Tree fern; Cryopreservation; Abiotic factor Ciências Biológicas.
Ano: 2014
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/24318

Dynamics in global DNA methylation and endogenous polyamine levels during protocorm-like bodies induction of Cattleya tigrina A. Richard
Provedor de dados: 3
Autores: Almeida, Vivian; Fraga, Hugo Pacheco de Freitas; Navarro, Bruno Bachiega; Guerra, Miguel Pedro; Pescador, Rosete.
Cattleya tigrina is endemic to the Atlantic forest biome and classified as vulnerable in the Red Book of Brazilian Flora. In vitro techniques comprise valuable tools for the conservation of endangered plant species. The aim of the present study was to evaluate the morphological features, global DNA methylation levels and free polyamines during protocorm-like bodies (PLBs) induction of C. tigrina. Along with that, an efficient protocol for in vitro propagation of this species is proposed. The first evidences of PLBs induction in C. tigrina occurred at seven days in culture, starting from the basal portion of the leaf abaxial surface. A hypomethylation marked the beginning of cell differentiation, followed by an increased global DNA methylation at 35 days in...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: 2.03.00.00-0; 2.03.03.00-9; 2.03.06.00-8 orchids; Micropropagation; In vitro culture; Epigenetics; PLBs. Botânica.
Ano: 2017
URL: http://periodicos.uem.br/ojs/index.php/ActaSciBiolSci/article/view/36656

Ultra-low temperature conservation of Brazilian Pine embryogenic cultures
Provedor de dados: 42
Autores: DEMARCHI,GRASIELA; STEFENON,VALDIR M.; STEINER,NEUSA; VIEIRA,FELIPE N.; VESCO,LIRIO L. DAL; GUERRA,MIGUEL P.
This study aimed to establish a cryopreservation protocol for embryogenic cultures of A. angustifolia, enabling the ex situ conservation of the species. Embryogenic cultures were established from immature seeds and treated with variations of the cryoprotectant solutions SuDG, SoD and PVS2 prior to immersion in liquid nitrogen. Cell viability was evaluated after 30, 60 and 90 days of re-growth. The highest re-growth without morphological alterations and with normal biochemical composition was obtained with the PVS2 solution with 40 min immersion in ethanol (-20 °C). This procedure opens new horizons for the ex situ conservation of the species genetic.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cryopreservation; Ex situ conservation; In vitro culture; Plant germplasm conservation.
Ano: 2014
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37652014000402057

Effect of somatotropin on survival and diameter of bovine preantral follicles
Provedor de dados: 45
Autores: Silva,T.F.; Costa,S.L.; Costa,E.P.; Guimarães,J.D.; Queiroz-Castro,V.L.D.
ABSTRACT The aim of this study was to evaluate the effect of Recombinant bovine somatotropin (rbST) on survival and diameter of bovine preantral ovarian follicles (PAOF) cultured in vitro. Ovaries were collected from adult cows and fragments of ovarian cortex were immediately fixed (non-cultured control) or cultured in vitro in α-MEM+ alone or containing 10, 50, 100 or 1,000ng/mL rbST. The fragments were processed for Classical Histology and Transmission Electron Microscopy. After one and seven days of culture, the percentage of normal follicles in the non-cultured control was superior (P&lt; 0.05) to the follicles cultured in α-MEM+ alone or with different rbST concentrations. The oocyte and follicular mean diameter did not increase during the culture for...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine; In vitro culture; Somatotropin; Preantral follicles.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352019000501445

Optimization of a protocol for direct organogenesis of red clover (Trifolium pratense L.) meristems for breeding purposes
Provedor de dados: 48
Autores: CARRILLO,JUAN C; OJEDA,VERÓNICA A; CAMPOS-DE QUIROZ,HUGO A; ORTEGA,FERNANDO M.
A series of experiments were carried out in order to optimize a protocol for the direct organogenesis of Chilean red clover germplasm. A range of cultivars were used to analyze the effect of explant source (crown or stem meristems of vegetative plants), culture media and plant growth regulators. Our findings showed that stem meristems were easier to obtain, presented lower levels of contamination and a better development than crown meristems. The L2 medium showed better results than B5 and MS media for the cultivars and experimental lines studied. L2 medium supplemented with 0.003 mg/l of 4-amino-3,5,6-trichloropicolinic acid and 1.0 mg/l of 6-benzylaminopurine gave consistently better results and will be applied in our breeding program to propagate,...
Tipo: Journal article
Palavras-chave: In vitro culture; Meristem culture; Red clover; Trifolium pratense L.
Ano: 2004
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602004000100005

Micropropagation of Endangered and Decorative Species Dianthus pinifolius Sibth. et Sm.
Provedor de dados: 52
Autores: Markovic,Marija; Grbic,Mihailo; Djukic,Matilda.
The aim of this study was to establish an efficient protocol for the in vitro propagation of the endangered, endemic and decorative species Dianthus pinifolius Sibth. et Sm. The effects of different concentrations of 6-benzylaminopurine (BAP) and naphtalenacetic acid (NAA), and different explant types (single-node cuttings, terminal buds and shoot cuttings) on shoot multiplication were examined on Murashige and Skoog (MS) and half-strength MS media. The best results were obtained for shoot cuttings on the MS medium supplemented with 0.5 mg/L BAP and 0.1 mg/L NAA, achieving a maximum rate of regeneration (100%) and a total of 15.4 newly-developed shoots per explant. The highest rooting rate (96.7%) was obtained on MS medium containing 1 mg/L NAA, while the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Pineleaf pink; In vitro culture; BAP; NAA; Multiplication.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100403

Generalized lineal models for the analysis of binary data from propagation experiments of Brazilian orchids
Provedor de dados: 52
Autores: Mora,Freddy; Gonçalves,Letícia de Menezes; Scapim,Carlos Alberto; Martins,Elias Nunes; Machado,Maria de Fátima Pires da Silva.
This study aimed at applying the generalized linear models (GLM) for the analysis of a germination experiment of Cattleya bicolor in which the response variable was binary. The purpose of this experiment was to assess the effects of the storage temperatures and culture mediums on the seed viability. The analyses of variance was also carried out either with or without the data transformation. All the statistical approaches indicated the importance of the storage temperature on the seed viability. But, the culture media and interaction effects were significant only by the GLM. Based on the GLM, the seeds stored at 10°C increased viability, in which the coconut medium achieved the best performance. The results emphasized the importance of adopting the GLM to...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cattleya bicolor; Deviance; In vitro culture; Logit model; Maximum-likelihood.
Ano: 2008
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132008000500013

In vitro culture of Vriesea gigantea and Vriesea philippocoburgii: two vulnerable bromeliads native to Southern Brazil
Provedor de dados: 52
Autores: Droste,Annette; Silva,Anelise Machado da; Matos,Adriana Vieira; Almeida,Júlia Winck de.
Micropropagation studies were carried out using the seeds for establishing an in vitro culture of Vriesea gigantea and Vriesea philippocoburgii. Germination rate of V. gigantea was higher than of V. philippocoburgii. Plantlets of V. philippocoburgii gave rise to many adventitious shoots when cultivated in Knudson basal medium. In contrast, for V. gigantea, a higher salts-concentration was needed, so that the number of shoots was increased by Murashige and Skoog medium. Addition of activated charcoal and naphthaleneacetic acid in regeneration medium allowed the growth of shoots and the formation of roots, confirming the success of in vitro culture. The differences in expression of the genotypes reinforce the need of more research in order to set up the...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Conservation; Propagation; Bromeliaceae; Vriesea.
Ano: 2005
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132005000600006

Efficient In Vitro Somatic Embryogenesis and Plant Regeneration from Mature and Immature Embryos of Wheat ( Triticum aestivum L.)
Provedor de dados: 52
Autores: Ahmadpour,Roghayeh; Zare,Nasser; Asghari-Zakarta,Rasool; Sheikhzadeh,Parisa.
ABSTRACT An efficient regeneration system is a pre-requisite for the application of genetic transformation and functional genomics study of important plants. In this study, the effect of different factors (plant growth regulators, casein hydrolysate, aspartic acid and ascorbic acid) on in vitro embryogenesis and regeneration of Arta, Bahar and Zagros cultivars from mature and immature explants were investigated. Immature and mature embryos were dissected from disinfected seeds 20-25 days after pollination and imbedded mature seeds, respectively, and cultured on MS (Murashige and Skoog) medium supplemented with different compounds. The results showed that immature embryos expose high capacity of embryogenesis and regeneration in comparison with mature...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Plant growth regulators; Somatic embryogenesis; Wheat.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132016000100206

Effect of supplementation of green tea polyphenols on the developmental competence of bovine oocytes in vitro
Provedor de dados: 56
Autores: Wang,Z.G.; Yu,S.D.; Xu,Z.R.
The objective of the present study was to examine the effect of green tea polyphenols (GTPs) supplementation during in vitro maturation, in vitro fertilization, and in vitro culture on the developmental competence of bovine oocytes. Cumulus-oocyte complexes aspirated from the ovaries were matured in vitro (38.5ºC for 24 h) and fertilized (38.5ºC for 15-18 h) and embryos were cultured (38.5ºC for 192 h) in a defined conditioned medium with or without GTPs supplementation. The GTPs used in the present study contained 99% catechin derivatives, with the major components being 50% (-)-epigallocatechin gallate, 22% (-)-epicatechin gallate, 18% (-)-epigallocatechin, and 10% (-)-epicatechin. Four replicate trials were done for each type of experiment. GTPs...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bovine oocytes; In vitro culture; Green tea polyphenols; Embryo development.
Ano: 2007
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2007000800008

In vitro establishment and propagation of a Brazilian strain ofAnaplasma marginale with appendage in IDE8 (Ixodes scapularis) cells
Provedor de dados: 58
Autores: Bastos,Camila V.; Passos,Lygia M. F.; Vasconcelos,Maria Mercês C.; Ribeiro,Múcio F. B.
A Brazilian isolate of Anaplasma marginale with appendage was successfully established and maintained in vitro in a tick cell line (IDE8). Infection was confirmed by optical and transmission electron microscopy. In addition, primers MSP1aNF2 and MSP1aNR2 amplified products from DNA extracted from infected IDE8 cells. Comparisons with partial sequences of the msp1α gene and the complete genome of A. marginale confirmed that the sequences of amplified fragments were from the A. marginale genome. This is the first establishment of a Brazilian A. marginale isolate in tick cells, representing a new system for biological and molecular studies and also a new source of material for diagnosis and development of vaccines.
Tipo: Info:eu-repo/semantics/article
Palavras-chave: IDE8 cells; Anaplasma marginale; In vitro culture.
Ano: 2009
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000200034

Agrobacterium rhizogenes-mediated transformation of Hypericum sinaicum L. for the development of hairy roots containing hypericin
Provedor de dados: 60
Autores: Khlifa,Heba Desouky; Klimek-Chodacka,Magdalena; Baranski,Rafal; Combik,Michal; Taha,Hussein Sayed.
Hypericum sinaicum L. is an endangered Egyptian medicinal plant of high importance due to the presence of naphthodianthrones (hypericins), which have photodynamic properties and pharmaceutical potential. We sought to assess H. sinaicum ability to develop hairy roots that could be cultured in contained conditions in vitro and used as a source for hypericin production. We used four A. rhizogenes strains differing in their plasmids and chromosomal backgrounds to inoculate excised H. sinaicum root, stem and leaf explants to induce hairy root development. Additionally, inoculum was applied to shoots held in Rockwool cubes supporting their stand after removal of the root system. All explant types were susceptible to A. rhizogenes although stem explants responded...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Medicinal plant; Naphthodianthrones; Plant genetic engineering.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502020000100590

Management of Auxin-Cytokinin Interactions to Improve Micropropagation Protocol of Henequen (Agave fourcroydes Lem.)
Provedor de dados: 117
Autores: Garriga Caraballo,Miguel; González Oramas,Gerardo; Alemán García,Silvia; Abreu Cruz,Enildo; Quiroz Bravo,Karla; D.S. Caligari,Peter; García-González,Rolando.
Henequen (Agave fourcroydes Lem.) is a high value plant species both for agricultural and ecological uses. The species is very well known worldwide by the quality of its fiber that is widely used for industrial purposes. This species is propagated at a large scale by asexual methods but current propagation technologies are not satisfying the grower’s demands. A. fourcroydes has been cultivated n vitro but it has shown some recalcitrant behaviour during the multiplication and rooting stages. The combination of 6-benzylamynopurine (BAP) (0.75 mg L-1) and indolebutyric acid (IBA) (1.0 mg L-1) instead of 2,4-dichlorophenoxyacetic acid (2,4-D) significantly improved explant survival and shooting during the establishment of n vitro young shoots. Combining...
Tipo: Journal article
Palavras-chave: Propagation; Thidiazuron; 6-benzylamynopurine; In vitro culture.
Ano: 2010
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-58392010000400003

Effect of the inoculum size on the axenic in vitro production of Steinernema carpocapsae
Provedor de dados: 118
Autores: Suárez-Sánchez,Juan; Alatorre-Rosas,Raquel; Poggi-Varaldo,Héctor M; Barrera-Cortés,Josefina.
The effect of inoculum size on the axenic in vitro production of Steinernema carpocapsae, using a transparent medium with hemoglobin and cholesterol, was studied. Axenic cultivation was performed with fertile eggs ofS. carpocapsae (hatching of eggs: 89 ± 4%), which were obtained by lyses of female nematodes and subsequent incubation. Two important aspects of this work include the use of a completely transparent medium enriched with hemoglobin and cholesterol and the high multiplication factor of the population (MFIP) of 87, which is considered high for in vitro axenic cultures of nematodes. In vivo methods have the advantage of high MFIP, but they also have the disadvantage of the high number and complexity of the operations involved for the purposes of...
Tipo: Journal article
Palavras-chave: Axenic culture; Hemoglobin; In vitro culture; Steinernema carpocapsae; Transparent medium.
Ano: 2012
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202012000100011

In vitro culture of Luma chequen from vegetative buds
Provedor de dados: 118
Autores: Mancilla,Héctor; Quiroz,Karla; Arencibia,Ariel; Carrasco,Basilio; García-Gonzales,Rolando.
H. Mancilla, K. Quiroz, A. Arencibia, B. Carrasco, and R. García-Gonzales. 2013. In vitro culture of Luma chequen from vegetative buds. Cien. Inv. Agr. 40(3): 609-615. Luma chequen, a small tree or large shrub belonging to the Myrtaceae family, is endemic to South America and has medicinal, nutritional and ornamental potential. However, its native habitat is deteriorating gradually, and it is suffering from the effects of fragmentation that is being caused by the conversion of forest land to agricultural land and the natural expansion of monocultural plantations of exotic species, such as Pinus radiata. The purpose of this work is to develop an effective procedure for establishing in vitro cultures of the native Chilean species L. chequen. Aseptic nodal...
Tipo: Journal article
Palavras-chave: Aseptic nodal segments; In vitro culture; Myrtaceae family; Organogenesis.
Ano: 2013
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202013000300013

Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp.
Provedor de dados: 118
Autores: Bastías,Adriana; Almada,Rubén; Rojas,Pamela; Donoso,José Manuel; Hinrichsen,Patricio; Sagredo,Boris.
A. Bastías, R. Almada, P. Rojas, J.M. Donoso, P. Hinrichsen, and B. Sagredo. 2016. Aging gene pathway of microRNAs 156/157 and 172 is altered in juvenile and adult plants from in vitro propagated Prunus sp. Cien. Inv. Agr. 43(3):429-441. In vitro culture is a very popular technique to mass propagate valuable plant genotypes, including Prunus sp. cultivars. Plants that undergo tissue culture processes often change their morphology and behavior due to the "rejuvenation" caused by the plant growth regulators included in the medium. To evaluate the effects of rejuvenation by tissue culture in Prunus sp., the expression patterns of the aging gene pathway described in plant models, which include the highly conserved microRNA (miRNA or miR) 156/157 and 172...
Tipo: Journal article
Palavras-chave: Development; In vitro culture; Hase change; Rejuvenation.
Ano: 2016
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202016000300009

Vernonia condensata Baker: an alternative for large-scale seedling production
Provedor de dados: 65
Autores: Almeida,Lilia Vieira da Silva; Oliveira,Vania Jesus dos Santos de; Jacobi,Claudia Cecilia Blaszkowski de; Almeida,Weliton Antonio Bastos de; Carvalho,Mariane de Jesus da Silva de.
ABSTRACT: The increasing use of Vernonia condensata Baker highlights the importance of developing strategies to reduce the impact of exploitation on nature reserves. The aim of this study was to establish a micropropagation protocol to produce homogenous plants with high phytosanitary quality. Apical, nodal, and internodal segments of plants grown in the field were used for in vitro growth. The segments were disinfected in sodium hypochlorite solution (1.0 and 2.0%) for 15 and 30 minutes and then transferred to Petri dishes containing MS culture medium for 30 days. A completely randomized factorial experiment (3 x 2 x 2) with five replicates was designed. After this period, a completely randomized in vitro multiplication experiment was carried out with six...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cytokinin; In vitro culture; Environmental sustainability.
Ano: 2020
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782020000300201

Cattleya walkeriana growth in different micropropagation systems
Provedor de dados: 65
Autores: Moreira,André Luís; Silva,Adriano Bortolotti da; Santos,Aline; Reis,Caroline Oliveira dos; Landgraf,Paulo Roberto Correa.
The aim of the present research was to verify the in vitro growth of orchids in different systems of micropropagation, being cultivated in a bioreactor, with natural ventilation and conventional systems. Cattleya walkeriana plants were obtained from the germination of seeds in culture medium. After 8 months, seedlings with 1 cm of length were placed in a culture vessel according to the treatments, which counted with two micropropagation systems (conventional and natural ventilation) in three media of culture (liquid, solid with 5 or 6g L-1 of agar). Two additional treatments in bioreactor of temporary and continuous immersion were performed. The design was entirely randomized (ERD), consisting of a 2x3 factorial with two additional treatments, totaling 8...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Bioreactor; Temporary immersion; Liquid medium; Natural ventilation; In vitro culture.
Ano: 2013
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782013001000012

Carbohydrates on the growth of Cattleya schilleriana Reichb.f. seedlings (Orchidaceae)
Provedor de dados: 65
Autores: Galdiano Júnior,Renato Fernandes; Mantovani,Cibele; Lemos,Eliana Gertrudes de Macedo.
ABSTRACT: The aim of the present study was to evaluate the effects of carbohydrate supplementation on the propagation of the orchid Cattleya schilleriana. The 120-d-old seedlings were subcultured in fructose-, glucose-, or sucrose-supplemented (0, 15, 30, and 45g L-1) ½ MS culture medium (half-strength macronutrient concentrations), using a completely random design with four repetitions per treatment. After 120d of treatment, root number and length, leaf number and length, and fresh weight were evaluated, and seedling survival was evaluated after 75d of acclimatization in a greenhouse. The in vitro growth data were submitted to regression analysis, whereas the percentage survival data were analyzed using ANOVA and Tukey’s test. Both in vitro growth and ex...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: In vitro culture; Native orchid; Glucose; Sucrose; Acclimatization.
Ano: 2018
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000700203

Micropropagation of the new apple rootstock ‘G. 814’
Provedor de dados: 65
Autores: Meneguzzi,Aline; Gonçalves,Mayra Juline; Camargo,Samila Silva; Grimaldi,Fernanda; Weber,Gabriela Candido; Rufato,Leo.
ABSTRACT: International breeding programs launched new genetic material of apple rootstocks that in addition to precocity and great yield are resistant to major diseases and soil pests encountered in the largest apple producing regions in Brazil. Given this, there is a necessity for vegetative propagation of these materials for study and possible replacement of existing rootstocks. The objective was to adapt a micropropagation protocol for new apple rootstock ‘G. 814’. In the multiplication phase were evaluated BAP concentrations: 0; 0.5; 1; 2 and 4mg L-1 and in the rooting phase were evaluated IBA concentrations: 0; 0.25; 0.50; 1; 1.5 and 2.5mg L-1. These new results demonstrated that this new rootstock selection can be propagated with this tissue culture...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Malus sp.; In vitro culture; Plant growth regulators.
Ano: 2017
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000600401

Anatomical and histochemical aspects of zigotic embryo and leaves in 'Coqueiro Anão'
Provedor de dados: 65
Autores: Santana,Marlucia Cruz de; Souza,Margarete Magalhães; Pereira,Telma Nair Santana; Teixeira,Sílvio Lopes.
The paper provides information about histochemical staining reactions in leaves and embryos of 'Coqueiro Anão' (Cocos nucifera). It was compared in vitro coconut and autotrophic palm leaves. Reactions for insoluble polysaccharides and acidic compounds, protein, extractable lipids, lignin and other classes of compounds were tested using histochemical tests. None sample gave positive reaction for lignin and phenolic compounds. All the samples gave positive reaction for protein, starch and insoluble polysaccharides while acidic compounds were positive only in in vitro leaves. Both in vitro and autotrophic leaves gave positive reaction for lipids showing presence of cuticle even in in vitro leaves. Only autotrophic palm leaves showed idioblasts containing...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Cocos nucifera; In vitro culture; Polysaccharides; Protein; Lipids.
Ano: 2010
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782010000400019

Towards the micropropagation of Euphorbia cyathophora Murray: a wild plant species with medicinal and ornamental potential
Provedor de dados: 65
Autores: Rodríguez-Elizalde,María de los Ángeles; Ferrera-Cerrato,Ronald; Hernández,Mateo Vargas; León,María Teresa Beryl Colinas y; González,Alejandro Manzo; Alarcón,Alejandro.
ABSTRACT:  Euphorbia cyathophora Murray is a plant species with medicinal and ornamental potential but whose in vitro propagation is unknown. Two experiments were performed to know 1) the effect of light (WL) at 25±2 °C and 20 μmol m-2 s-1 luminous intensity, or darkness at 20±2 °C, for the first 20 days and subsequent placement in light (DKL) as previously described, and 2) the best combination of plant growth regulators: benzyl amino purine (BAP), kynetin (KIN), 2-isopentenyl adenine (2iP) and thidiazuron (TDZ) in combination with naphthaleneacetic acid (NAA) and the effect of both light conditions. The DKL treatment did not promote the growth of leaves, cotyledons, or plantlet height; in contrast, WL produced a more developed root system with early...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Euphorbiaceae; In vitro culture; Phytohormones; Light conditions.
Ano: 2019
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782019000900402

Optimization of jenipapo in vitro seed germination process
Provedor de dados: 64
Autores: Souza,Rafaela Ribeiro de; Paiva,Patrícia Duarte de Oliveira; Silva,Raphael Reis da; Reis,Michele Valquíria dos; Nery,Fernanda Carlota; Paiva,Renato.
ABSTRACT The in vitro seed germination is an effective alternative for quickly obtaining explants with sanitary quality. However, jenipapo seeds present slow and uneven germination. Therefore, internal and external factors to seed which directly interfere in the process, they must be identified, in order to adapt better techniques to obtain seedlings. In this sense, this work aimed to optimize the in vitro germination of Genipa americana L. seeds by evaluating different factors (light quality, GA3 treatment, pre-soaking in distilled water, growing media and stratification in the dark). It was found that the seed germination of G. americana was indifferent to light, however, the best results were obtained under conditions of continuous darkness; There was...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genipa americana L; In vitro culture; Ligth quality; Rubiaceae; Stratification in the dark.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000600658

In vitro propagation and acclimatization of genipapo accessions
Provedor de dados: 64
Autores: Sa,Francielen Paola de; Ledo,Ana da Silva; Amorim,Julie Anne Espindola; Silva,Ana Veruska Cruz da; Pasqual,Moacir.
ABSTRACT Genipapo is a species with an intermediate seed storage behavior, and its germination is slow and asynchronous; therefore the tissue culture is an alternative strategy for the propagation in large-scale. This study aimed to evaluate three genipapo accessions at different micropropagation stages (adventitious shoot induction, in vitro rooting and acclimatization). Genipapo nodal segments from the Sabinópolis (AS), Cruz das Almas (CZA) and Núcleo Bandeirante (NB) accessions were used and inoculated in culture media with 0 and 1.0 mg L-1 6-benzylaminopurine (BAP). Different indole-3-butyric acid (IBA) concentrations were tested for in vitro rooting. Subsequently, the effect of two substrates on the seedling development of three genipapo accessions...
Tipo: Info:eu-repo/semantics/article
Palavras-chave: Genipa americana; In vitro culture; Micropropagation.
Ano: 2016
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-70542016000200155